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The map label for this gene is dnaG [H]

Identifier: 154686782

GI number: 154686782

Start: 2471054

End: 2472865

Strand: Reverse

Name: dnaG [H]

Synonym: RBAM_023520

Alternate gene names: 154686782

Gene position: 2472865-2471054 (Counterclockwise)

Preceding gene: 154686783

Following gene: 154686781

Centisome position: 63.11

GC content: 48.34

Gene sequence:

>1812_bases
ATGGGAAACCGCATACCAGATGAAGTAGTTAATCAGGTGCAGCAGTCAGCAGACATTGTTGAAGTCATAGGTGATTATGT
CCAATTGAAAAAGCAAGGCCGCAACTACTTTGGGCTCTGTCCGTTTCACGGGGAAAACACGCCCTCGTTTTCCGTCTCCA
GCGATAAGCAGATCTTTCATTGCTTCGGATGCGGTGCGGGCGGGAACGTTTTCTCTTTCTTACGGCAGATGGAAGGTTAT
TCATTTACGGAAGCTGTTTCTCACCTTGCGGATAAATATCAGATTGATTTCCCTGATGATATAACGATTCACTCTGCATC
CAGGCCGGAATCTTCCGGAGAGCAGAAGATGGCGGAAGCCCACGAGCTTCTGAAGAAATTTTACCATCATTTGCTAATAA
ATACAAAGGAAGGCCAGGAAGCATTAGATTACCTTTTATCAAGGGGTTTTACGAAAGAGCTCATAGATGAGTTTGAAATC
GGCTACGCGCTGGATTCCTGGGATTTCATGACGAAATTCTTGAAAAAGCGCGGTTTTGAGGAAGAACTGATGGAAAAAGC
GGGTCTGCTGATCAGAAGGGAAGACGGCAGCGGGTATTTTGACCGTTTCAGAAACAGAATTATGTTTCCCATTCATGATC
ACCACGGAGCCGTGGCGGCTTTTTCAGGAAGGGCGCTCGGAAGCCAGCAGCCGAAATATATGAACAGCCCGGAAACGCCG
CTGTTTCATAAATCTAAGCTGCTGTATAACTTTCATAAGGCGCGCCTGCCGATCAGGAAAGAGGAGAGAGCCGTTCTGTT
TGAAGGCTTCGCTGACGTCATATCCGCCGTCAGCTCGGGCGTAAAAGAAAGTATCGCCACAATGGGGACGTCGCTCACCG
AAGAACATGTGAAAATCCTCAGGAGAAATGTTCAGGAGGTCATTCTCTGCTACGATTCGGATAAAGCCGGGTACGAAGCT
ACGCTGAAAGCGGCCGGCCTGCTCCAGAAAGCCGATTGTAAGGTAAGAGTAAGCATGCTGCCTGACGGACTTGATCCTGA
TGATTATATCAGAATCCGGGGCGGAGAAAAATTCCGGACCGATATCATTGATGCAAGCGTTACGGTCATGGCTTTTAAAA
TGCAGTATTTCCGCAAAGGGAAAAACTTATCTGACGAAGGAGACCGGCTCGCTTACATGAAAGAAGTGCTGAAAGAAATC
AGCGCTCTTTCCGGGTCGCTTGAGCAGGAAATGTATGTGAAGCAGCTCGCCTCAGAATTTTCTCTTTCGGTAGAATCTTT
GACGGAACAGCTTTCCATCTACGCCAGACAAAACGCCCCCGGCGACAAAAACGCCGAACCGCAGACAAGGCGGGCCCGGG
TGACGACAAAAGCGAAACGGCGCCGTCTCCGTCCCGCCTATGAGAACGCGGAAAGACTTCTGCTCGCTCATATGCTGAGA
GACCGAAACGTCATAAAGAAAGTGATTGAGCGTGTCGGCTTTGAATTTAACATTGACGGACATCGTGCGCTGGCAGCATA
CCTGTACGCTGTTTATGAAGAGGGCGCTGACATGACGCCGCAGCATCTTATGGCGAGAATGCAGGATGAGGAAATCAGCC
AGCTGCTTTCAGATATAGGGATGCTCCAAGTAAACGAGGAGCTCTCAGAGGCCGAGTTATCCGATTACGTAAAAAAAGTG
TTGGATCACCGAAAATGGTCAATGATAAAGGAAAAAGAAGCAGAAAGAGCGGATGCGGAGAGGCAGAAAGACTTCGTGAA
AGCTGCGTCTCTGGCTCAAGAAATTGTTGCATTGAACCGATCATTAAAGTAA

Upstream 100 bases:

>100_bases
AGATCGGAATCGATTTGTTACCCTGCTTAAAAAAATCCTGTCGAATGATGAAGGGATTTCAAATCAAAACATCGAATAAT
GTACGACGGAGTGTTATAAG

Downstream 100 bases:

>100_bases
CACCAAAAAGAGGCTTGCGTTCTGATTGGCAATGATTCCTTGCGGAGGAGCAAATAGATCGCTTAACCTCATCATGAATT
GTCATTTATTATCCGCAGCC

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 603; Mature: 602

Protein sequence:

>603_residues
MGNRIPDEVVNQVQQSADIVEVIGDYVQLKKQGRNYFGLCPFHGENTPSFSVSSDKQIFHCFGCGAGGNVFSFLRQMEGY
SFTEAVSHLADKYQIDFPDDITIHSASRPESSGEQKMAEAHELLKKFYHHLLINTKEGQEALDYLLSRGFTKELIDEFEI
GYALDSWDFMTKFLKKRGFEEELMEKAGLLIRREDGSGYFDRFRNRIMFPIHDHHGAVAAFSGRALGSQQPKYMNSPETP
LFHKSKLLYNFHKARLPIRKEERAVLFEGFADVISAVSSGVKESIATMGTSLTEEHVKILRRNVQEVILCYDSDKAGYEA
TLKAAGLLQKADCKVRVSMLPDGLDPDDYIRIRGGEKFRTDIIDASVTVMAFKMQYFRKGKNLSDEGDRLAYMKEVLKEI
SALSGSLEQEMYVKQLASEFSLSVESLTEQLSIYARQNAPGDKNAEPQTRRARVTTKAKRRRLRPAYENAERLLLAHMLR
DRNVIKKVIERVGFEFNIDGHRALAAYLYAVYEEGADMTPQHLMARMQDEEISQLLSDIGMLQVNEELSEAELSDYVKKV
LDHRKWSMIKEKEAERADAERQKDFVKAASLAQEIVALNRSLK

Sequences:

>Translated_603_residues
MGNRIPDEVVNQVQQSADIVEVIGDYVQLKKQGRNYFGLCPFHGENTPSFSVSSDKQIFHCFGCGAGGNVFSFLRQMEGY
SFTEAVSHLADKYQIDFPDDITIHSASRPESSGEQKMAEAHELLKKFYHHLLINTKEGQEALDYLLSRGFTKELIDEFEI
GYALDSWDFMTKFLKKRGFEEELMEKAGLLIRREDGSGYFDRFRNRIMFPIHDHHGAVAAFSGRALGSQQPKYMNSPETP
LFHKSKLLYNFHKARLPIRKEERAVLFEGFADVISAVSSGVKESIATMGTSLTEEHVKILRRNVQEVILCYDSDKAGYEA
TLKAAGLLQKADCKVRVSMLPDGLDPDDYIRIRGGEKFRTDIIDASVTVMAFKMQYFRKGKNLSDEGDRLAYMKEVLKEI
SALSGSLEQEMYVKQLASEFSLSVESLTEQLSIYARQNAPGDKNAEPQTRRARVTTKAKRRRLRPAYENAERLLLAHMLR
DRNVIKKVIERVGFEFNIDGHRALAAYLYAVYEEGADMTPQHLMARMQDEEISQLLSDIGMLQVNEELSEAELSDYVKKV
LDHRKWSMIKEKEAERADAERQKDFVKAASLAQEIVALNRSLK
>Mature_602_residues
GNRIPDEVVNQVQQSADIVEVIGDYVQLKKQGRNYFGLCPFHGENTPSFSVSSDKQIFHCFGCGAGGNVFSFLRQMEGYS
FTEAVSHLADKYQIDFPDDITIHSASRPESSGEQKMAEAHELLKKFYHHLLINTKEGQEALDYLLSRGFTKELIDEFEIG
YALDSWDFMTKFLKKRGFEEELMEKAGLLIRREDGSGYFDRFRNRIMFPIHDHHGAVAAFSGRALGSQQPKYMNSPETPL
FHKSKLLYNFHKARLPIRKEERAVLFEGFADVISAVSSGVKESIATMGTSLTEEHVKILRRNVQEVILCYDSDKAGYEAT
LKAAGLLQKADCKVRVSMLPDGLDPDDYIRIRGGEKFRTDIIDASVTVMAFKMQYFRKGKNLSDEGDRLAYMKEVLKEIS
ALSGSLEQEMYVKQLASEFSLSVESLTEQLSIYARQNAPGDKNAEPQTRRARVTTKAKRRRLRPAYENAERLLLAHMLRD
RNVIKKVIERVGFEFNIDGHRALAAYLYAVYEEGADMTPQHLMARMQDEEISQLLSDIGMLQVNEELSEAELSDYVKKVL
DHRKWSMIKEKEAERADAERQKDFVKAASLAQEIVALNRSLK

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=455, Percent_Identity=34.7252747252747, Blast_Score=271, Evalue=7e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007693
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 68707; Mature: 68576

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNRIPDEVVNQVQQSADIVEVIGDYVQLKKQGRNYFGLCPFHGENTPSFSVSSDKQIFH
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCEEE
CFGCGAGGNVFSFLRQMEGYSFTEAVSHLADKYQIDFPDDITIHSASRPESSGEQKMAEA
EECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCHHHHHHHH
HELLKKFYHHLLINTKEGQEALDYLLSRGFTKELIDEFEIGYALDSWDFMTKFLKKRGFE
HHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCEECCHHHHHHHHHHCCCH
EELMEKAGLLIRREDGSGYFDRFRNRIMFPIHDHHGAVAAFSGRALGSQQPKYMNSPETP
HHHHHHCCCEEEECCCCCHHHHHCCCEEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCC
LFHKSKLLYNFHKARLPIRKEERAVLFEGFADVISAVSSGVKESIATMGTSLTEEHVKIL
CHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
RRNVQEVILCYDSDKAGYEATLKAAGLLQKADCKVRVSMLPDGLDPDDYIRIRGGEKFRT
HHHHHHEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEEEECCCCHHHH
DIIDASVTVMAFKMQYFRKGKNLSDEGDRLAYMKEVLKEISALSGSLEQEMYVKQLASEF
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
SLSVESLTEQLSIYARQNAPGDKNAEPQTRRARVTTKAKRRRLRPAYENAERLLLAHMLR
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
DRNVIKKVIERVGFEFNIDGHRALAAYLYAVYEEGADMTPQHLMARMQDEEISQLLSDIG
HHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHCC
MLQVNEELSEAELSDYVKKVLDHRKWSMIKEKEAERADAERQKDFVKAASLAQEIVALNR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SLK
CCC
>Mature Secondary Structure 
GNRIPDEVVNQVQQSADIVEVIGDYVQLKKQGRNYFGLCPFHGENTPSFSVSSDKQIFH
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCEEE
CFGCGAGGNVFSFLRQMEGYSFTEAVSHLADKYQIDFPDDITIHSASRPESSGEQKMAEA
EECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCHHHHHHHH
HELLKKFYHHLLINTKEGQEALDYLLSRGFTKELIDEFEIGYALDSWDFMTKFLKKRGFE
HHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCEECCHHHHHHHHHHCCCH
EELMEKAGLLIRREDGSGYFDRFRNRIMFPIHDHHGAVAAFSGRALGSQQPKYMNSPETP
HHHHHHCCCEEEECCCCCHHHHHCCCEEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCC
LFHKSKLLYNFHKARLPIRKEERAVLFEGFADVISAVSSGVKESIATMGTSLTEEHVKIL
CHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
RRNVQEVILCYDSDKAGYEATLKAAGLLQKADCKVRVSMLPDGLDPDDYIRIRGGEKFRT
HHHHHHEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEEEECCCCHHHH
DIIDASVTVMAFKMQYFRKGKNLSDEGDRLAYMKEVLKEISALSGSLEQEMYVKQLASEF
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
SLSVESLTEQLSIYARQNAPGDKNAEPQTRRARVTTKAKRRRLRPAYENAERLLLAHMLR
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
DRNVIKKVIERVGFEFNIDGHRALAAYLYAVYEEGADMTPQHLMARMQDEEISQLLSDIG
HHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHCC
MLQVNEELSEAELSDYVKKVLDHRKWSMIKEKEAERADAERQKDFVKAASLAQEIVALNR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SLK
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3919021; 3086839; 8969508; 9384377 [H]