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The map label for this gene is dfnA [H]

Identifier: 154686639

GI number: 154686639

Start: 2344008

End: 2346266

Strand: Reverse

Name: dfnA [H]

Synonym: RBAM_022080

Alternate gene names: 154686639

Gene position: 2346266-2344008 (Counterclockwise)

Preceding gene: 154686641

Following gene: 154686638

Centisome position: 59.88

GC content: 46.83

Gene sequence:

>2259_bases
ATGCTTACGTTTGTGTTCCCGGGGCAGGGTTCTCAATTTAAAGGAATGGGAGCCGGACTGTTTGATGAATTTCAAGATCT
GACACGTCAGGCTGATGACATATTAGGCTACTCTATAGAAGAATTGTGTCTGGAGGATCCGAATCATCAGCTGGGGAAAA
CGCAATTTACCCAGCCGGCGTTATATACGGTGAGTGCACTCAGTTATTTAAAGAAAATGAAAGAGTCCGGCAGAGAGCCT
GATTACGCCGCAGGGCACAGTTTAGGAGAATATAATGCGTTATTTGCGGCCGGCTGCTTTGATTTTGAGACCGGTTTGCA
GCTTGTGAAAAAAAGAGGGGAGCTCATGAGCAAGGCAGCTCCCGGAGGCATGGCGGCGGTTTTGGGGTTTACCGCTGAAC
AAGTAAAAGAGGTTCTATCCGATTATCATCTGACCGGCATTGATATCGCTAACCACAATTCACCTTCGCAAATTGTGATC
GCCGGAACGAAACAAGACATTGAAAAAGCGGGCCCCGTCTTTGAAAAAGCCGGCGTGAGAATGTACCTTCCTTTAAATGT
GAGCGGCGCCTTCCATTCCCGCTATATGAAAGATGCTGAAAAAGAGTTTGCAGATTATTTAGAAGAAACGGCGTTTTTGC
CTCTTCGTTTTCCGGTGATCTCAAATCTTCATGCCGCACCTTATAAGAATGATGAAATCAAAACAAATCTGACTTTGCAA
ATGACCAATCAGGTAAAGTGGACGGACACGATACGCCGTCTGATGGGACTTGAAAATAATGAAATTGCAGAAGTCGGGCC
CGGGGAAGTGCTGACAAAACTGACGCGTCAGATTAAAAAAGATGCCGTCCCATTACCTATGCCGAAAGAAGAGTCTGATA
CTGCTGATGTTAAAGCATCGGCAGCTGATCCGCAAAAAACGGCGGGAATGAGATTAGGGAACGAAGATTTTAAAAAAGAT
TATAACATCCAATATGCTTACATGACGGGCTCCATGTACAGGGGAATTGCTTCGGAACAGATGGTCATTAAAGCGGCAAA
AGCGGGAATGCTGGGCTTTTTCGGAACGGGCGGATTGAGTATCGAACGGATCGGACAGGCCATCGGAACCATCCGGTCTG
CACTGCGGCAAGGGGAAACATTCGGAATGAATCTTCTTCATCATATGATGTCACCTGACAAGGAAGTCAGAATGATAGAT
TTATATTTGAAAAACGGCATTCATCTTATTGAAGCATCGGCATTTATGGGCATAACCCCCGCTCTTGTTATATACAGGGC
AAAGGGATTAAGCAGGAATCATGACGGGTCGGTGTCAGTTCAAAACAAAATCATCGCGAAAGTATCCCGGCCTGAAGTCG
CTGAGGCCTTTCTGAATCCGGCTCCGGCTCATGTGCTGGAAAGACTTGTGTCTGACAATCGGCTGACAGCCGGGGAAGCG
GCGCTTGCAAAAGAAATTCCGATGGCTGATGACATTTGCGTTGAAGCGGATTCAGGAGGGCATACCGACCAAGGGATTCC
GTATACATTAATGCCTGCCATGATCAGACTCAGAGACCGCATGATGGAGAAGCACGGTTATGCGAAAAAAGTAAGAATCG
GCGCAGCGGGCGGCATCGGCACTCCGGAAGCGGCTGCTGCCGCGTTTCTGCTCGGCGCCGAATTTATCGGAACCGGTTCT
ATTAATCAATGCACGGTCGAAGCCGGGACAAGTGATTCAGTGAAAGATCTGCTTCAGGAAGCGAACGTTCAGGATACGTC
ATACGCTCCCGCGGGAGATATGTTTGAAGCGGGAGCAAGGGTGCAGGTGCTGAAAAAAGGCCTGTTTTTCCCGGCAAGAG
CCAATAAACTCTTTGACTTGTACAGACAATACAATTCTTTAGATGAAATAGATGAAAAAACGAAAACATTAATTGAAGAA
AAGTATTTCCAGCGCAGTTTTGAAGAAGTGTATGAGCAGTTGAAACGTGATAAATCTCCTGAGCAGATCGCCAAAGCCGA
ACAGAATCCGAAACACAAAATGGCGATGGTATTTAAATGGTATTTTTCTCATACGACAAGGCTCGCTCTGGAAGGAAAAA
GCGAAAGTAAAATAGATTACCAAATTCATTGCGGCCCTGCGCTCGGCGCGTTTAATCAATGGGTGAAAGGAACCCCGCTT
GAAAATTGGAGAAACAGGCATGTCGATCTGATCGGCAAACAGCTCATGGAAGAAACAGCCGGCCTTCTTGCGCAAAGGCT
GGTATCGATTACAGGCTGA

Upstream 100 bases:

>100_bases
CCGGTGTTTACACCAGTCAATATATGAAATAAGAGACGTTTTTGTATGGAGATCCACATGTGATATACAAAATTGGCGAT
GCGGAAAGGATGAGGATAAG

Downstream 100 bases:

>100_bases
GTATGACCAGCCATTACATTTTTTCTGCGGAGGACAGACCATGCTGATTGAAAATCAGGAATTAACGATGAGCCATGTCG
GGTTAGGGAATTTGAATGAG

Product: DfnA

Products: NA

Alternate protein names: Malonyl CoA-acyl carrier protein transacylase; MCT [H]

Number of amino acids: Translated: 752; Mature: 752

Protein sequence:

>752_residues
MLTFVFPGQGSQFKGMGAGLFDEFQDLTRQADDILGYSIEELCLEDPNHQLGKTQFTQPALYTVSALSYLKKMKESGREP
DYAAGHSLGEYNALFAAGCFDFETGLQLVKKRGELMSKAAPGGMAAVLGFTAEQVKEVLSDYHLTGIDIANHNSPSQIVI
AGTKQDIEKAGPVFEKAGVRMYLPLNVSGAFHSRYMKDAEKEFADYLEETAFLPLRFPVISNLHAAPYKNDEIKTNLTLQ
MTNQVKWTDTIRRLMGLENNEIAEVGPGEVLTKLTRQIKKDAVPLPMPKEESDTADVKASAADPQKTAGMRLGNEDFKKD
YNIQYAYMTGSMYRGIASEQMVIKAAKAGMLGFFGTGGLSIERIGQAIGTIRSALRQGETFGMNLLHHMMSPDKEVRMID
LYLKNGIHLIEASAFMGITPALVIYRAKGLSRNHDGSVSVQNKIIAKVSRPEVAEAFLNPAPAHVLERLVSDNRLTAGEA
ALAKEIPMADDICVEADSGGHTDQGIPYTLMPAMIRLRDRMMEKHGYAKKVRIGAAGGIGTPEAAAAAFLLGAEFIGTGS
INQCTVEAGTSDSVKDLLQEANVQDTSYAPAGDMFEAGARVQVLKKGLFFPARANKLFDLYRQYNSLDEIDEKTKTLIEE
KYFQRSFEEVYEQLKRDKSPEQIAKAEQNPKHKMAMVFKWYFSHTTRLALEGKSESKIDYQIHCGPALGAFNQWVKGTPL
ENWRNRHVDLIGKQLMEETAGLLAQRLVSITG

Sequences:

>Translated_752_residues
MLTFVFPGQGSQFKGMGAGLFDEFQDLTRQADDILGYSIEELCLEDPNHQLGKTQFTQPALYTVSALSYLKKMKESGREP
DYAAGHSLGEYNALFAAGCFDFETGLQLVKKRGELMSKAAPGGMAAVLGFTAEQVKEVLSDYHLTGIDIANHNSPSQIVI
AGTKQDIEKAGPVFEKAGVRMYLPLNVSGAFHSRYMKDAEKEFADYLEETAFLPLRFPVISNLHAAPYKNDEIKTNLTLQ
MTNQVKWTDTIRRLMGLENNEIAEVGPGEVLTKLTRQIKKDAVPLPMPKEESDTADVKASAADPQKTAGMRLGNEDFKKD
YNIQYAYMTGSMYRGIASEQMVIKAAKAGMLGFFGTGGLSIERIGQAIGTIRSALRQGETFGMNLLHHMMSPDKEVRMID
LYLKNGIHLIEASAFMGITPALVIYRAKGLSRNHDGSVSVQNKIIAKVSRPEVAEAFLNPAPAHVLERLVSDNRLTAGEA
ALAKEIPMADDICVEADSGGHTDQGIPYTLMPAMIRLRDRMMEKHGYAKKVRIGAAGGIGTPEAAAAAFLLGAEFIGTGS
INQCTVEAGTSDSVKDLLQEANVQDTSYAPAGDMFEAGARVQVLKKGLFFPARANKLFDLYRQYNSLDEIDEKTKTLIEE
KYFQRSFEEVYEQLKRDKSPEQIAKAEQNPKHKMAMVFKWYFSHTTRLALEGKSESKIDYQIHCGPALGAFNQWVKGTPL
ENWRNRHVDLIGKQLMEETAGLLAQRLVSITG
>Mature_752_residues
MLTFVFPGQGSQFKGMGAGLFDEFQDLTRQADDILGYSIEELCLEDPNHQLGKTQFTQPALYTVSALSYLKKMKESGREP
DYAAGHSLGEYNALFAAGCFDFETGLQLVKKRGELMSKAAPGGMAAVLGFTAEQVKEVLSDYHLTGIDIANHNSPSQIVI
AGTKQDIEKAGPVFEKAGVRMYLPLNVSGAFHSRYMKDAEKEFADYLEETAFLPLRFPVISNLHAAPYKNDEIKTNLTLQ
MTNQVKWTDTIRRLMGLENNEIAEVGPGEVLTKLTRQIKKDAVPLPMPKEESDTADVKASAADPQKTAGMRLGNEDFKKD
YNIQYAYMTGSMYRGIASEQMVIKAAKAGMLGFFGTGGLSIERIGQAIGTIRSALRQGETFGMNLLHHMMSPDKEVRMID
LYLKNGIHLIEASAFMGITPALVIYRAKGLSRNHDGSVSVQNKIIAKVSRPEVAEAFLNPAPAHVLERLVSDNRLTAGEA
ALAKEIPMADDICVEADSGGHTDQGIPYTLMPAMIRLRDRMMEKHGYAKKVRIGAAGGIGTPEAAAAAFLLGAEFIGTGS
INQCTVEAGTSDSVKDLLQEANVQDTSYAPAGDMFEAGARVQVLKKGLFFPARANKLFDLYRQYNSLDEIDEKTKTLIEE
KYFQRSFEEVYEQLKRDKSPEQIAKAEQNPKHKMAMVFKWYFSHTTRLALEGKSESKIDYQIHCGPALGAFNQWVKGTPL
ENWRNRHVDLIGKQLMEETAGLLAQRLVSITG

Specific function: Probably involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism. Probably has an acyl transferase activity and could also have a flavin mononucleotide-dependent oxidoreductas

COG id: COG2070

COG function: function code R; Dioxygenases related to 2-nitropropane dioxygenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the fabD family [H]

Homologues:

Organism=Homo sapiens, GI51243059, Length=305, Percent_Identity=33.1147540983607, Blast_Score=122, Evalue=1e-27,
Organism=Homo sapiens, GI41872631, Length=209, Percent_Identity=29.1866028708134, Blast_Score=70, Evalue=1e-11,
Organism=Escherichia coli, GI1787334, Length=285, Percent_Identity=37.5438596491228, Blast_Score=165, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI71984332, Length=293, Percent_Identity=27.6450511945392, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17550940, Length=290, Percent_Identity=26.8965517241379, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24665753, Length=305, Percent_Identity=30.1639344262295, Blast_Score=110, Evalue=3e-24,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004136
- InterPro:   IPR001227
- InterPro:   IPR014043
- InterPro:   IPR016035
- InterPro:   IPR013785
- InterPro:   IPR004410
- InterPro:   IPR016036
- InterPro:   IPR014179 [H]

Pfam domain/function: PF00698 Acyl_transf_1; PF03060 NPD [H]

EC number: =2.3.1.39 [H]

Molecular weight: Translated: 82953; Mature: 82953

Theoretical pI: Translated: 6.48; Mature: 6.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTFVFPGQGSQFKGMGAGLFDEFQDLTRQADDILGYSIEELCLEDPNHQLGKTQFTQPA
CEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCH
LYTVSALSYLKKMKESGREPDYAAGHSLGEYNALFAAGCFDFETGLQLVKKRGELMSKAA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHCC
PGGMAAVLGFTAEQVKEVLSDYHLTGIDIANHNSPSQIVIAGTKQDIEKAGPVFEKAGVR
CCCHHHHHHCCHHHHHHHHHCCCCCEEEECCCCCCCEEEEECCHHHHHHHCCHHHHCCCE
MYLPLNVSGAFHSRYMKDAEKEFADYLEETAFLPLRFPVISNLHAAPYKNDEIKTNLTLQ
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECHHHHCCCCCCCCCCCEEEEEEEE
MTNQVKWTDTIRRLMGLENNEIAEVGPGEVLTKLTRQIKKDAVPLPMPKEESDTADVKAS
ECCCEEHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
AADPQKTAGMRLGNEDFKKDYNIQYAYMTGSMYRGIASEQMVIKAAKAGMLGFFGTGGLS
CCCCHHHHCCCCCCHHHCCCCCEEEEEECCHHHHCCCHHHHHHHHHHCCCEEEECCCCCC
IERIGQAIGTIRSALRQGETFGMNLLHHMMSPDKEVRMIDLYLKNGIHLIEASAFMGITP
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEEHHHCCCEEEEHHHHHCCCH
ALVIYRAKGLSRNHDGSVSVQNKIIAKVSRPEVAEAFLNPAPAHVLERLVSDNRLTAGEA
HHHHHHHCCCCCCCCCCEEHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHCCCCCCCCHH
ALAKEIPMADDICVEADSGGHTDQGIPYTLMPAMIRLRDRMMEKHGYAKKVRIGAAGGIG
HHHHCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHEEEEECCCCCC
TPEAAAAAFLLGAEFIGTGSINQCTVEAGTSDSVKDLLQEANVQDTSYAPAGDMFEAGAR
CCHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCHHHCCCH
VQVLKKGLFFPARANKLFDLYRQYNSLDEIDEKTKTLIEEKYFQRSFEEVYEQLKRDKSP
HHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
EQIAKAEQNPKHKMAMVFKWYFSHTTRLALEGKSESKIDYQIHCGPALGAFNQWVKGTPL
HHHHHHCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCEEEEEECCHHHHHHHHHHCCCCC
ENWRNRHVDLIGKQLMEETAGLLAQRLVSITG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLTFVFPGQGSQFKGMGAGLFDEFQDLTRQADDILGYSIEELCLEDPNHQLGKTQFTQPA
CEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCH
LYTVSALSYLKKMKESGREPDYAAGHSLGEYNALFAAGCFDFETGLQLVKKRGELMSKAA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHCC
PGGMAAVLGFTAEQVKEVLSDYHLTGIDIANHNSPSQIVIAGTKQDIEKAGPVFEKAGVR
CCCHHHHHHCCHHHHHHHHHCCCCCEEEECCCCCCCEEEEECCHHHHHHHCCHHHHCCCE
MYLPLNVSGAFHSRYMKDAEKEFADYLEETAFLPLRFPVISNLHAAPYKNDEIKTNLTLQ
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECHHHHCCCCCCCCCCCEEEEEEEE
MTNQVKWTDTIRRLMGLENNEIAEVGPGEVLTKLTRQIKKDAVPLPMPKEESDTADVKAS
ECCCEEHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
AADPQKTAGMRLGNEDFKKDYNIQYAYMTGSMYRGIASEQMVIKAAKAGMLGFFGTGGLS
CCCCHHHHCCCCCCHHHCCCCCEEEEEECCHHHHCCCHHHHHHHHHHCCCEEEECCCCCC
IERIGQAIGTIRSALRQGETFGMNLLHHMMSPDKEVRMIDLYLKNGIHLIEASAFMGITP
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEEHHHCCCEEEEHHHHHCCCH
ALVIYRAKGLSRNHDGSVSVQNKIIAKVSRPEVAEAFLNPAPAHVLERLVSDNRLTAGEA
HHHHHHHCCCCCCCCCCEEHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHCCCCCCCCHH
ALAKEIPMADDICVEADSGGHTDQGIPYTLMPAMIRLRDRMMEKHGYAKKVRIGAAGGIG
HHHHCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHEEEEECCCCCC
TPEAAAAAFLLGAEFIGTGSINQCTVEAGTSDSVKDLLQEANVQDTSYAPAGDMFEAGAR
CCHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCHHHCCCH
VQVLKKGLFFPARANKLFDLYRQYNSLDEIDEKTKTLIEEKYFQRSFEEVYEQLKRDKSP
HHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
EQIAKAEQNPKHKMAMVFKWYFSHTTRLALEGKSESKIDYQIHCGPALGAFNQWVKGTPL
HHHHHHCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCEEEEEECCHHHHHHHHHHCCCCC
ENWRNRHVDLIGKQLMEETAGLLAQRLVSITG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]