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The map label for this gene is ribE [H]

Identifier: 154686572

GI number: 154686572

Start: 2234305

End: 2234952

Strand: Reverse

Name: ribE [H]

Synonym: RBAM_021410

Alternate gene names: 154686572

Gene position: 2234952-2234305 (Counterclockwise)

Preceding gene: 154686573

Following gene: 154686571

Centisome position: 57.03

GC content: 45.37

Gene sequence:

>648_bases
ATGTTTACAGGAATCGTTGAAGAAACAGGCACGATCCAAGCAATCAAAAAGACAGGCCTTTCCATGGCATTAACCATCGC
CGCTTCAAAAGTGATAAGTGATGTGAAACTTGGCGACAGCATTGCGGTAAACGGCATCTGCCTGACCGTAACCGGATTTT
CTGACAATCAATTTACGGTTGATGTCATGCCGGAAACAGTGAAAGCCACTTCGCTGAACGGCTTGTCAAAAGGCAGTAAA
GTAAACCTGGAAAGAGCGATGTCCGCCAACGGACGGTTCGGCGGCCACTTTGTATCAGGGCATGTGGACGGAACGGCGGA
AATTACGCGGATCGAGAAAAAAAGCAACGCGGTCTATTACGACTTAAAACTTTCGCCGGAATTGACAAAAACGCTCGTAT
TGAAAGGTTCAATTACGGTAGACGGCGTGAGCCTGACGATTTTCGGTTTATCAGACGAAAGCGTAACGGTATCCATCATT
CCGCATACCATCTCAGAAACGATTTTTCAGACGAAAACAGTCGGATCGACCGTAAATATTGAGTGTGACATGATCGGGAA
ATACTTATACCGCTTTTTGCACAAAACAGAACAAACGAAAAGCAATCAAACTATCACGGAAGCCTTTTTAAGCGAGAACG
GCTTTTAG

Upstream 100 bases:

>100_bases
TCAATGAAAGATGTCCCCCATTTACAATTCACACAAATCACCCAAATCGGGCCGGATATAAAACTGACGGCCATACCTAA
AGATGGAAAGGATGGTGACG

Downstream 100 bases:

>100_bases
AAAGGAAGATCGATATGTTTCATCCGATAGAAGAGGCGTTAGAGGCGCTGAAAAAAGGTGAAGTCATCATTGTTGTCGAT
GATGAAGACAGAGAAAACGA

Product: riboflavin synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MFTGIVEETGTIQAIKKTGLSMALTIAASKVISDVKLGDSIAVNGICLTVTGFSDNQFTVDVMPETVKATSLNGLSKGSK
VNLERAMSANGRFGGHFVSGHVDGTAEITRIEKKSNAVYYDLKLSPELTKTLVLKGSITVDGVSLTIFGLSDESVTVSII
PHTISETIFQTKTVGSTVNIECDMIGKYLYRFLHKTEQTKSNQTITEAFLSENGF

Sequences:

>Translated_215_residues
MFTGIVEETGTIQAIKKTGLSMALTIAASKVISDVKLGDSIAVNGICLTVTGFSDNQFTVDVMPETVKATSLNGLSKGSK
VNLERAMSANGRFGGHFVSGHVDGTAEITRIEKKSNAVYYDLKLSPELTKTLVLKGSITVDGVSLTIFGLSDESVTVSII
PHTISETIFQTKTVGSTVNIECDMIGKYLYRFLHKTEQTKSNQTITEAFLSENGF
>Mature_215_residues
MFTGIVEETGTIQAIKKTGLSMALTIAASKVISDVKLGDSIAVNGICLTVTGFSDNQFTVDVMPETVKATSLNGLSKGSK
VNLERAMSANGRFGGHFVSGHVDGTAEITRIEKKSNAVYYDLKLSPELTKTLVLKGSITVDGVSLTIFGLSDESVTVSII
PHTISETIFQTKTVGSTVNIECDMIGKYLYRFLHKTEQTKSNQTITEAFLSENGF

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The alpha subunit catalyze

COG id: COG0307

COG function: function code H; Riboflavin synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lumazine-binding repeats [H]

Homologues:

Organism=Escherichia coli, GI1787952, Length=186, Percent_Identity=35.4838709677419, Blast_Score=113, Evalue=8e-27,
Organism=Saccharomyces cerevisiae, GI6319733, Length=203, Percent_Identity=40.8866995073892, Blast_Score=142, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001783
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00677 Lum_binding [H]

EC number: =2.5.1.9 [H]

Molecular weight: Translated: 23070; Mature: 23070

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00693 LUM_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTGIVEETGTIQAIKKTGLSMALTIAASKVISDVKLGDSIAVNGICLTVTGFSDNQFTV
CCCCCCCCCCCHHHHHHCCCEEEEEHHHHHHHHHHCCCCCEEECCEEEEEEECCCCEEEE
DVMPETVKATSLNGLSKGSKVNLERAMSANGRFGGHFVSGHVDGTAEITRIEKKSNAVYY
EECCCEEEEECCCCCCCCCEECHHHHHCCCCCCCCEEEEEECCCCEEEEEEECCCCEEEE
DLKLSPELTKTLVLKGSITVDGVSLTIFGLSDESVTVSIIPHTISETIFQTKTVGSTVNI
EEEECCCCEEEEEEECCEEECCEEEEEEEECCCCEEEEEECCHHHHHHHHHHCCCCEEEE
ECDMIGKYLYRFLHKTEQTKSNQTITEAFLSENGF
EHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MFTGIVEETGTIQAIKKTGLSMALTIAASKVISDVKLGDSIAVNGICLTVTGFSDNQFTV
CCCCCCCCCCCHHHHHHCCCEEEEEHHHHHHHHHHCCCCCEEECCEEEEEEECCCCEEEE
DVMPETVKATSLNGLSKGSKVNLERAMSANGRFGGHFVSGHVDGTAEITRIEKKSNAVYY
EECCCEEEEECCCCCCCCCEECHHHHHCCCCCCCCEEEEEECCCCEEEEEEECCCCEEEE
DLKLSPELTKTLVLKGSITVDGVSLTIFGLSDESVTVSIIPHTISETIFQTKTVGSTVNI
EEEECCCCEEEEEEECCEEECCEEEEEEEECCCCEEEEEECCHHHHHHHHHHCCCCEEEE
ECDMIGKYLYRFLHKTEQTKSNQTITEAFLSENGF
EHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9297088 [H]