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The map label for this gene is ribA

Identifier: 154686571

GI number: 154686571

Start: 2233094

End: 2234290

Strand: Reverse

Name: ribA

Synonym: RBAM_021400

Alternate gene names: 154686571

Gene position: 2234290-2233094 (Counterclockwise)

Preceding gene: 154686572

Following gene: 154686570

Centisome position: 57.02

GC content: 50.29

Gene sequence:

>1197_bases
ATGTTTCATCCGATAGAAGAGGCGTTAGAGGCGCTGAAAAAAGGTGAAGTCATCATTGTTGTCGATGATGAAGACAGAGA
AAACGAAGGAGATTTCGTAGCGTTGGCTGAGCATGCCACGCCGGAAGTGGTGAATTTTATGGCGACCCATGGTAGAGGCC
TGATCTGCACGCCGCTGTCTGAAGACATCGCCGGCCGGCTGGATCTTCACCCGATGGTCGATCATAATACGGACTCACAT
GAGACCGCGTTTACGGTCAGTATTGACCACAGGCTGACAAAAACGGGCATCAGCGCGCAGGAACGATCCTTTACCATTCA
GGCGCTGTTGAACGAAGAATCTGTGTCTGACGATTTTCAGCGTCCGGGCCACATTTTCCCCTTAATCGCAAAAAAAGGAG
GAGTCCTGAAGCGGGCCGGCCATACGGAAGCAGCCGTTGACCTTGCAAAAGCCTGCGGATCTCAGGGAGCGGGCGTCATT
TGTGAAATTATGAATGAAGACGGCACAATGGCCAGAGTGCCGGAGTTAGCCGAAATCGCCGAACGCCACCAGCTGAAAAT
GATTACGATAAAAGACTTAATAGAGTACCGTTACAACATTACGACACTTGTGAACAGAGAAGTTGATATTACGCTGCCGA
CTGACTTCGGCACGTTTAGAGTTTACGGATATACAAACGAGGTTGACGGAAAAGAACATCTCGCCTTTGTCATGGGGGAT
GTCCCGTTTAACAGCGAACCCGTTCTTGTCAGAGTGCACTCAGAATGCCTGACGGGCGATGTGTTTGCATCTCATCGCTG
TGATTGCGGACCTCAGCTTCATGCCGCGCTTGCGCAAATTGCTGAAGAAGGCCGCGGCGTTCTATTGTATTTGCGCCAGG
AAGGCAGAGGAATCGGTTTAATCAATAAGCTGAAGGCGTACCGATTGCAGGAACAAGGGTATGACACGGTTGAAGCGAAC
GAAGCGCTCGGCTTTCTGCCTGATCTGCGCAACTACGGCATCGGCGCCCAGATTCTCCGCGACTTAGGGGTTCAGCATAT
GAAACTTTTAACCAATAACCCCCGGAAGATCGCCGGCCTCGAAGGATACGGTCTCAGCATTTCAGAACGGGTGCCGCTTC
AAATGGAAGCGAGTGAGCATAACAAGCAATATTTACAGACCAAAATGAAAAAACTTGGACATCTGCTTCATTTCTAA

Upstream 100 bases:

>100_bases
ACTTATACCGCTTTTTGCACAAAACAGAACAAACGAAAAGCAATCAAACTATCACGGAAGCCTTTTTAAGCGAGAACGGC
TTTTAGAAAGGAAGATCGAT

Downstream 100 bases:

>100_bases
AAACAAATCTCACAAAAAAGGATGGATAACATATGAATATCATACAAGGAAACCTAGTAGGAACAGGACTTAAAATCGGA
ATCGTCGTCGGCAGATTTAA

Product: bifunctional 3,4-dihydroxy-2-butanone 4-phosphate synthase/GTP cyclohydrolase II protein

Products: NA

Alternate protein names: 3,4-dihydroxy-2-butanone 4-phosphate synthase; DHBP synthase; GTP cyclohydrolase-2; GTP cyclohydrolase II

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MFHPIEEALEALKKGEVIIVVDDEDRENEGDFVALAEHATPEVVNFMATHGRGLICTPLSEDIAGRLDLHPMVDHNTDSH
ETAFTVSIDHRLTKTGISAQERSFTIQALLNEESVSDDFQRPGHIFPLIAKKGGVLKRAGHTEAAVDLAKACGSQGAGVI
CEIMNEDGTMARVPELAEIAERHQLKMITIKDLIEYRYNITTLVNREVDITLPTDFGTFRVYGYTNEVDGKEHLAFVMGD
VPFNSEPVLVRVHSECLTGDVFASHRCDCGPQLHAALAQIAEEGRGVLLYLRQEGRGIGLINKLKAYRLQEQGYDTVEAN
EALGFLPDLRNYGIGAQILRDLGVQHMKLLTNNPRKIAGLEGYGLSISERVPLQMEASEHNKQYLQTKMKKLGHLLHF

Sequences:

>Translated_398_residues
MFHPIEEALEALKKGEVIIVVDDEDRENEGDFVALAEHATPEVVNFMATHGRGLICTPLSEDIAGRLDLHPMVDHNTDSH
ETAFTVSIDHRLTKTGISAQERSFTIQALLNEESVSDDFQRPGHIFPLIAKKGGVLKRAGHTEAAVDLAKACGSQGAGVI
CEIMNEDGTMARVPELAEIAERHQLKMITIKDLIEYRYNITTLVNREVDITLPTDFGTFRVYGYTNEVDGKEHLAFVMGD
VPFNSEPVLVRVHSECLTGDVFASHRCDCGPQLHAALAQIAEEGRGVLLYLRQEGRGIGLINKLKAYRLQEQGYDTVEAN
EALGFLPDLRNYGIGAQILRDLGVQHMKLLTNNPRKIAGLEGYGLSISERVPLQMEASEHNKQYLQTKMKKLGHLLHF
>Mature_398_residues
MFHPIEEALEALKKGEVIIVVDDEDRENEGDFVALAEHATPEVVNFMATHGRGLICTPLSEDIAGRLDLHPMVDHNTDSH
ETAFTVSIDHRLTKTGISAQERSFTIQALLNEESVSDDFQRPGHIFPLIAKKGGVLKRAGHTEAAVDLAKACGSQGAGVI
CEIMNEDGTMARVPELAEIAERHQLKMITIKDLIEYRYNITTLVNREVDITLPTDFGTFRVYGYTNEVDGKEHLAFVMGD
VPFNSEPVLVRVHSECLTGDVFASHRCDCGPQLHAALAQIAEEGRGVLLYLRQEGRGIGLINKLKAYRLQEQGYDTVEAN
EALGFLPDLRNYGIGAQILRDLGVQHMKLLTNNPRKIAGLEGYGLSISERVPLQMEASEHNKQYLQTKMKKLGHLLHF

Specific function: Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate

COG id: COG0807

COG function: function code H; GTP cyclohydrolase II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the GTP cyclohydrolase II family

Homologues:

Organism=Escherichia coli, GI1787533, Length=194, Percent_Identity=54.1237113402062, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI1789420, Length=197, Percent_Identity=46.7005076142132, Blast_Score=188, Evalue=7e-49,
Organism=Saccharomyces cerevisiae, GI6320695, Length=206, Percent_Identity=41.747572815534, Blast_Score=164, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6319438, Length=170, Percent_Identity=46.4705882352941, Blast_Score=127, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIBBA_BACA2 (A7Z675)

Other databases:

- EMBL:   CP000560
- RefSeq:   YP_001421732.1
- ProteinModelPortal:   A7Z675
- STRING:   A7Z675
- EnsemblBacteria:   EBBACT00000007095
- GeneID:   5462558
- GenomeReviews:   CP000560_GR
- KEGG:   bay:RBAM_021400
- eggNOG:   COG0108
- GeneTree:   EBGT00050000001077
- HOGENOM:   HBG735778
- OMA:   RCDCRMQ
- ProtClustDB:   PRK09311
- BioCyc:   BAMY326423:RBAM_021400-MONOMER
- HAMAP:   MF_01283
- InterPro:   IPR017945
- InterPro:   IPR000422
- InterPro:   IPR000926
- InterPro:   IPR016299
- Gene3D:   G3DSA:3.90.870.10
- PIRSF:   PIRSF001259
- TIGRFAMs:   TIGR00505
- TIGRFAMs:   TIGR00506

Pfam domain/function: PF00926 DHBP_synthase; PF00925 GTP_cyclohydro2; SSF55821 DHBP_synth_RibB-like_a/b_dom

EC number: =4.1.99.12; =3.5.4.25

Molecular weight: Translated: 44171; Mature: 44171

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: NA

Important sites: ACT_SITE 328-328 ACT_SITE 330-330 BINDING 31-31 BINDING 162-162 BINDING 272-272 BINDING 316-316 BINDING 351-351 BINDING 356-356

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFHPIEEALEALKKGEVIIVVDDEDRENEGDFVALAEHATPEVVNFMATHGRGLICTPLS
CCCCHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEECCCCHHHHHHHHHCCCEEEECCCC
EDIAGRLDLHPMVDHNTDSHETAFTVSIDHRLTKTGISAQERSFTIQALLNEESVSDDFQ
HHHCCCCCCCCCCCCCCCCCCEEEEEEECCCHHHCCCCCHHHCEEEEEECCCCCCCHHHC
RPGHIFPLIAKKGGVLKRAGHTEAAVDLAKACGSQGAGVICEIMNEDGTMARVPELAEIA
CCCCEEEEEECCCCCEEECCCHHHHHHHHHHHCCCCCCEEEEEECCCCCEECCCHHHHHH
ERHQLKMITIKDLIEYRYNITTLVNREVDITLPTDFGTFRVYGYTNEVDGKEHLAFVMGD
HHHCEEEEEHHHHHHHHCCEEEEECCEEEEEECCCCCCEEEEEECCCCCCCCEEEEEEEC
VPFNSEPVLVRVHSECLTGDVFASHRCDCGPQLHAALAQIAEEGRGVLLYLRQEGRGIGL
CCCCCCCEEEEEECCHHCCCHHHCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCH
INKLKAYRLQEQGYDTVEANEALGFLPDLRNYGIGAQILRDLGVQHMKLLTNNPRKIAGL
HHHHHHHHHHHCCCCCCCCCCHHCCCCCHHHCCCHHHHHHHHCHHHHHHHCCCCCEEECC
EGYGLSISERVPLQMEASEHNKQYLQTKMKKLGHLLHF
CCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFHPIEEALEALKKGEVIIVVDDEDRENEGDFVALAEHATPEVVNFMATHGRGLICTPLS
CCCCHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEECCCCHHHHHHHHHCCCEEEECCCC
EDIAGRLDLHPMVDHNTDSHETAFTVSIDHRLTKTGISAQERSFTIQALLNEESVSDDFQ
HHHCCCCCCCCCCCCCCCCCCEEEEEEECCCHHHCCCCCHHHCEEEEEECCCCCCCHHHC
RPGHIFPLIAKKGGVLKRAGHTEAAVDLAKACGSQGAGVICEIMNEDGTMARVPELAEIA
CCCCEEEEEECCCCCEEECCCHHHHHHHHHHHCCCCCCEEEEEECCCCCEECCCHHHHHH
ERHQLKMITIKDLIEYRYNITTLVNREVDITLPTDFGTFRVYGYTNEVDGKEHLAFVMGD
HHHCEEEEEHHHHHHHHCCEEEEECCEEEEEECCCCCCEEEEEECCCCCCCCEEEEEEEC
VPFNSEPVLVRVHSECLTGDVFASHRCDCGPQLHAALAQIAEEGRGVLLYLRQEGRGIGL
CCCCCCCEEEEEECCHHCCCHHHCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCH
INKLKAYRLQEQGYDTVEANEALGFLPDLRNYGIGAQILRDLGVQHMKLLTNNPRKIAGL
HHHHHHHHHHHCCCCCCCCCCHHCCCCCHHHCCCHHHHHHHHCHHHHHHHCCCCCEEECC
EGYGLSISERVPLQMEASEHNKQYLQTKMKKLGHLLHF
CCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA