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The map label for this gene is rluB [H]

Identifier: 154686561

GI number: 154686561

Start: 2226009

End: 2226743

Strand: Reverse

Name: rluB [H]

Synonym: RBAM_021300

Alternate gene names: 154686561

Gene position: 2226743-2226009 (Counterclockwise)

Preceding gene: 154686562

Following gene: 154686560

Centisome position: 56.83

GC content: 47.89

Gene sequence:

>735_bases
ATGGAAAGACTGCAAAAAGTTATTGCTCATGCCGGCGTGGCATCACGCAGAAAGGCGGAAGAGCTGATAAAAGAAGGAAA
AGTAAAGGTGAATGGAAAAGTAATAACAGAGCTCGGCGTAAAAGTGACCGGTTCTGATCAGATTGAAGTAAACGGATTAA
AGGTGGAGCGGGAAGAACCCGTGTACTTTCTGCTTTATAAACCGAGAGGAGTGATCTCAGCAGCTGATGACGACAAAGGG
CGGAAAGTGGTGACCGACTTTTTCAAAGAAGTGCCGCAGCGCATTTATCCGATCGGCAGGCTTGATTATGATACGAGCGG
ACTTTTATTGCTGACAAATGACGGCGAGTTTGCCAACAAACTCATGCATCCGAAATACGAAATTGATAAGACGTACGTCG
CCAAAGTGAAGGGGATTCCTTCCAAGGAACTTCTGAGAAAGCTTGAGCGCGGCATTCAGCTTGAAGAAGGCAAGACGGCG
CCGGCCAGAGCGAAATTGCTTTCCCTGGACAAAAGAAAGCAGACGAGCATCATTCAGCTGACGATTCATGAAGGCCGAAA
CAGACAGGTCCGCCGCATGTTCGAGGCGATCGGTCATGACGTGCTGAAACTAAAACGTGAAGAATACGCATTTTTAGATT
TAAGAGGCCTGCGCACAGGAGACGCAAGAGAATTGTCGCCGCATGAGGTGAAAAGGCTGCGGGCTTTAGCTGAACACGGT
AAAAACGCTTTCTAA

Upstream 100 bases:

>100_bases
CGCCTGAAATCAGGCGCTTTTTATATATCAAAATAACTCTCCTTGAAAAAGCTTGTTTGTTCAAGGGGAAAAAAGTAAGA
TTATACTTGAGGTGAACGAC

Downstream 100 bases:

>100_bases
TTTTCACATAACCTTCAAAAATTACAAAACGTGTTGATGATATAATTGAATGGATTTGCAGGCAGGGGAGGGGAGCAGTG
TACAATGAACAAAAAACGGC

Product: RluB

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MERLQKVIAHAGVASRRKAEELIKEGKVKVNGKVITELGVKVTGSDQIEVNGLKVEREEPVYFLLYKPRGVISAADDDKG
RKVVTDFFKEVPQRIYPIGRLDYDTSGLLLLTNDGEFANKLMHPKYEIDKTYVAKVKGIPSKELLRKLERGIQLEEGKTA
PARAKLLSLDKRKQTSIIQLTIHEGRNRQVRRMFEAIGHDVLKLKREEYAFLDLRGLRTGDARELSPHEVKRLRALAEHG
KNAF

Sequences:

>Translated_244_residues
MERLQKVIAHAGVASRRKAEELIKEGKVKVNGKVITELGVKVTGSDQIEVNGLKVEREEPVYFLLYKPRGVISAADDDKG
RKVVTDFFKEVPQRIYPIGRLDYDTSGLLLLTNDGEFANKLMHPKYEIDKTYVAKVKGIPSKELLRKLERGIQLEEGKTA
PARAKLLSLDKRKQTSIIQLTIHEGRNRQVRRMFEAIGHDVLKLKREEYAFLDLRGLRTGDARELSPHEVKRLRALAEHG
KNAF
>Mature_244_residues
MERLQKVIAHAGVASRRKAEELIKEGKVKVNGKVITELGVKVTGSDQIEVNGLKVEREEPVYFLLYKPRGVISAADDDKG
RKVVTDFFKEVPQRIYPIGRLDYDTSGLLLLTNDGEFANKLMHPKYEIDKTYVAKVKGIPSKELLRKLERGIQLEEGKTA
PARAKLLSLDKRKQTSIIQLTIHEGRNRQVRRMFEAIGHDVLKLKREEYAFLDLRGLRTGDARELSPHEVKRLRALAEHG
KNAF

Specific function: Responsible for synthesis of pseudouridine from uracil- 2633 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=246, Percent_Identity=41.0569105691057, Blast_Score=145, Evalue=2e-36,
Organism=Escherichia coli, GI1790453, Length=243, Percent_Identity=32.9218106995885, Blast_Score=124, Evalue=5e-30,
Organism=Escherichia coli, GI1788510, Length=235, Percent_Identity=30.2127659574468, Blast_Score=118, Evalue=3e-28,
Organism=Escherichia coli, GI87081838, Length=187, Percent_Identity=36.3636363636364, Blast_Score=108, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 27765; Mature: 27765

Theoretical pI: Translated: 10.37; Mature: 10.37

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERLQKVIAHAGVASRRKAEELIKEGKVKVNGKVITELGVKVTGSDQIEVNGLKVEREEP
CHHHHHHHHHCCHHHHHHHHHHHHCCCEEECCEEEEEECEEECCCCEEEEECEEEECCCC
VYFLLYKPRGVISAADDDKGRKVVTDFFKEVPQRIYPIGRLDYDTSGLLLLTNDGEFANK
EEEEEECCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHH
LMHPKYEIDKTYVAKVKGIPSKELLRKLERGIQLEEGKTAPARAKLLSLDKRKQTSIIQL
HCCCCHHCCHHHEEHHCCCCHHHHHHHHHHCCEECCCCCCHHHHHHHHHCCCCCCEEEEE
TIHEGRNRQVRRMFEAIGHDVLKLKREEYAFLDLRGLRTGDARELSPHEVKRLRALAEHG
EEECCCCHHHHHHHHHHCHHHHHHHHHHEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCC
KNAF
CCCC
>Mature Secondary Structure
MERLQKVIAHAGVASRRKAEELIKEGKVKVNGKVITELGVKVTGSDQIEVNGLKVEREEP
CHHHHHHHHHCCHHHHHHHHHHHHCCCEEECCEEEEEECEEECCCCEEEEECEEEECCCC
VYFLLYKPRGVISAADDDKGRKVVTDFFKEVPQRIYPIGRLDYDTSGLLLLTNDGEFANK
EEEEEECCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHH
LMHPKYEIDKTYVAKVKGIPSKELLRKLERGIQLEEGKTAPARAKLLSLDKRKQTSIIQL
HCCCCHHCCHHHEEHHCCCCHHHHHHHHHHCCEECCCCCCHHHHHHHHHCCCCCCEEEEE
TIHEGRNRQVRRMFEAIGHDVLKLKREEYAFLDLRGLRTGDARELSPHEVKRLRALAEHG
EEECCCCHHHHHHHHHHCHHHHHHHHHHEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCC
KNAF
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934829; 9384377; 9888802 [H]