Click here to switch to the map view.

The map label for this gene is aprX [H]

Identifier: 154686140

GI number: 154686140

Start: 1774914

End: 1776242

Strand: Reverse

Name: aprX [H]

Synonym: RBAM_017070

Alternate gene names: 154686140

Gene position: 1776242-1774914 (Counterclockwise)

Preceding gene: 154686143

Following gene: 154686139

Centisome position: 45.33

GC content: 48.31

Gene sequence:

>1329_bases
ATGTTTGGTTACTCAATGGTACAAATGGTGCGGTCAAATGCTCATAAGCTGGACTGGCCGCTCAGAGAAAACGTATTACA
GTTATATAAACCTTTCAAATGGACACCTTGCTTTTTGCACAAGTTTTTCGAAAAAAAGGTGAAAAACAGAAAGAAAATGT
CAGTCATCATTGAATTTGAAGAGGGCTGCCATGAATCCGGTTTTCACAGCACAGGACAGGTCTTATCAAAAGAAAAACGC
TGCACCATAAAAAAGCAGTTTCAAACAATCAATTGCTGCAGCGCCGAAGTCACTCCGTCCGCCCTTCACATGCTTCTTTC
GCAATGCCGGGATATCCGTAAAATCTATTTAAACCGTGAAGTAAAAGCGCTCCTTGATACGGCAGCTGAATCGTCTCATG
CAAAAGAAGTGACCCGTAACGGCACCGTATTAACAGGAAAAGGCGTAACCGTCGCCGTTATTGATACCGGAATCTATCAG
CATCCCGATTTAGAAGGCAGAGTTATCGGATTTGCGGACTTTGTCAACCAAAAGACAGAACCGTATGATGACAACGGACA
CGGCACCCATTGCGCCGGTGATATCGCAAGCAGCGGCGCTTCATCTTCCGGTAAATATCAGGGGCCGGCGCCGGAAGCCG
ATCTGATCGGAGTCAAGGTATTAAACAAATCAGGATCAGGAACCTTGGCTGATATCATTGAAGGTGTGGAATGGTGCATA
CAATATAATAAAGAACACACAAAGAATCCGATCAGAATCATCAGCATGTCACTGGGCGGAGATGCGCTCAGATATGATAA
AGAAACGGACGACCCGCTCGTCAAGGCCGTTGAAAAGGCATGGAATGAAGGAATCGTCGTCTGCGTCGCCGCGGGCAACT
CCGGTCCGGAAGCTCAAACGATTTCAAGCCCCGGTGTCAGCGAGAAGGTCATCACAGTAGGCGCCTATGATGACAATGAT
ACGGCCGGCAATGAAGACGATACGGTCGCTTCTTTCTCAAGCCGCGGCCCGACGGTGTACGGAAAAGAGAAGCCTGACAT
TCTTGCGCCCGGGGTCGATATCGTATCTCTCCGTTCTCCGCGTTCATATCTTGACAAATTACAGAAATCAAACCGTGTAG
GCTCCTTGTATTTCAGTCTGTCGGGCACGTCCATGGCCACTCCGATCTGTGCGGGCATTGCCGCCTTAATCCTTCAGCAG
AACCCGCAGCTTTCGCCTGACGAAGTGAAAACACTGATCAAACAAAGTCCGGATCAATGGACAAATGAAGATCCGAACAT
TTACGGAGCGGGCGCAGTAAACGCTGAAAACGCCGTGCCGAAAGAGTAA

Upstream 100 bases:

>100_bases
CGCAAGTAACCAGCGCTCCTGAAGGACAAACAAACTGACCGAACATACGGCCTGTTCATATGGTATAGAGAGAATCGCCA
GAATGGGAGGAAAAGTATAT

Downstream 100 bases:

>100_bases
CCTTTTTATCATGAGAACGGGGTCGTCTGCACCGGCAGATGACTCCGTTTTTCAGGTGAACAGATGAAACATTTATAATG
AAGGAACTCGGAACGCTTCA

Product: AprX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MFGYSMVQMVRSNAHKLDWPLRENVLQLYKPFKWTPCFLHKFFEKKVKNRKKMSVIIEFEEGCHESGFHSTGQVLSKEKR
CTIKKQFQTINCCSAEVTPSALHMLLSQCRDIRKIYLNREVKALLDTAAESSHAKEVTRNGTVLTGKGVTVAVIDTGIYQ
HPDLEGRVIGFADFVNQKTEPYDDNGHGTHCAGDIASSGASSSGKYQGPAPEADLIGVKVLNKSGSGTLADIIEGVEWCI
QYNKEHTKNPIRIISMSLGGDALRYDKETDDPLVKAVEKAWNEGIVVCVAAGNSGPEAQTISSPGVSEKVITVGAYDDND
TAGNEDDTVASFSSRGPTVYGKEKPDILAPGVDIVSLRSPRSYLDKLQKSNRVGSLYFSLSGTSMATPICAGIAALILQQ
NPQLSPDEVKTLIKQSPDQWTNEDPNIYGAGAVNAENAVPKE

Sequences:

>Translated_442_residues
MFGYSMVQMVRSNAHKLDWPLRENVLQLYKPFKWTPCFLHKFFEKKVKNRKKMSVIIEFEEGCHESGFHSTGQVLSKEKR
CTIKKQFQTINCCSAEVTPSALHMLLSQCRDIRKIYLNREVKALLDTAAESSHAKEVTRNGTVLTGKGVTVAVIDTGIYQ
HPDLEGRVIGFADFVNQKTEPYDDNGHGTHCAGDIASSGASSSGKYQGPAPEADLIGVKVLNKSGSGTLADIIEGVEWCI
QYNKEHTKNPIRIISMSLGGDALRYDKETDDPLVKAVEKAWNEGIVVCVAAGNSGPEAQTISSPGVSEKVITVGAYDDND
TAGNEDDTVASFSSRGPTVYGKEKPDILAPGVDIVSLRSPRSYLDKLQKSNRVGSLYFSLSGTSMATPICAGIAALILQQ
NPQLSPDEVKTLIKQSPDQWTNEDPNIYGAGAVNAENAVPKE
>Mature_442_residues
MFGYSMVQMVRSNAHKLDWPLRENVLQLYKPFKWTPCFLHKFFEKKVKNRKKMSVIIEFEEGCHESGFHSTGQVLSKEKR
CTIKKQFQTINCCSAEVTPSALHMLLSQCRDIRKIYLNREVKALLDTAAESSHAKEVTRNGTVLTGKGVTVAVIDTGIYQ
HPDLEGRVIGFADFVNQKTEPYDDNGHGTHCAGDIASSGASSSGKYQGPAPEADLIGVKVLNKSGSGTLADIIEGVEWCI
QYNKEHTKNPIRIISMSLGGDALRYDKETDDPLVKAVEKAWNEGIVVCVAAGNSGPEAQTISSPGVSEKVITVGAYDDND
TAGNEDDTVASFSSRGPTVYGKEKPDILAPGVDIVSLRSPRSYLDKLQKSNRVGSLYFSLSGTSMATPICAGIAALILQQ
NPQLSPDEVKTLIKQSPDQWTNEDPNIYGAGAVNAENAVPKE

Specific function: Displays serine protease activity. Seems to have a broad substrate specificity [H]

COG id: COG1404

COG function: function code O; Subtilisin-like serine proteases

Gene ontology:

Cell location: Cytoplasm (Probable). Note=Is leaked to the culture medium during the late stationary phase owing to cell lysis [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S8 family [H]

Homologues:

Organism=Homo sapiens, GI4506775, Length=288, Percent_Identity=29.1666666666667, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI31317307, Length=305, Percent_Identity=29.8360655737705, Blast_Score=70, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6319893, Length=273, Percent_Identity=37.3626373626374, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6324576, Length=263, Percent_Identity=36.8821292775665, Blast_Score=117, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6320775, Length=263, Percent_Identity=35.7414448669202, Blast_Score=115, Evalue=1e-26,
Organism=Drosophila melanogaster, GI45550681, Length=314, Percent_Identity=31.2101910828025, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24653270, Length=269, Percent_Identity=30.4832713754647, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI17137360, Length=269, Percent_Identity=30.1115241635688, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000209
- InterPro:   IPR022398
- InterPro:   IPR015500 [H]

Pfam domain/function: PF00082 Peptidase_S8 [H]

EC number: NA

Molecular weight: Translated: 48147; Mature: 48147

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00136 SUBTILASE_ASP ; PS00137 SUBTILASE_HIS ; PS00138 SUBTILASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGYSMVQMVRSNAHKLDWPLRENVLQLYKPFKWTPCFLHKFFEKKVKNRKKMSVIIEFE
CCCHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEC
EGCHESGFHSTGQVLSKEKRCTIKKQFQTINCCSAEVTPSALHMLLSQCRDIRKIYLNRE
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHCHH
VKALLDTAAESSHAKEVTRNGTVLTGKGVTVAVIDTGIYQHPDLEGRVIGFADFVNQKTE
HHHHHHHHHHHHHHHHHHCCCCEEECCCCEEEEEECCCCCCCCCCCEEEEHHHHHCCCCC
PYDDNGHGTHCAGDIASSGASSSGKYQGPAPEADLIGVKVLNKSGSGTLADIIEGVEWCI
CCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHH
QYNKEHTKNPIRIISMSLGGDALRYDKETDDPLVKAVEKAWNEGIVVCVAAGNSGPEAQT
HHCHHHCCCCEEEEEEECCCCCEECCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCCC
ISSPGVSEKVITVGAYDDNDTAGNEDDTVASFSSRGPTVYGKEKPDILAPGVDIVSLRSP
CCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHCCCCEEECCCCCCEECCCCCEEEECCC
RSYLDKLQKSNRVGSLYFSLSGTSMATPICAGIAALILQQNPQLSPDEVKTLIKQSPDQW
HHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCC
TNEDPNIYGAGAVNAENAVPKE
CCCCCCEEECCCCCCCCCCCCC
>Mature Secondary Structure
MFGYSMVQMVRSNAHKLDWPLRENVLQLYKPFKWTPCFLHKFFEKKVKNRKKMSVIIEFE
CCCHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEC
EGCHESGFHSTGQVLSKEKRCTIKKQFQTINCCSAEVTPSALHMLLSQCRDIRKIYLNRE
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHCHH
VKALLDTAAESSHAKEVTRNGTVLTGKGVTVAVIDTGIYQHPDLEGRVIGFADFVNQKTE
HHHHHHHHHHHHHHHHHHCCCCEEECCCCEEEEEECCCCCCCCCCCEEEEHHHHHCCCCC
PYDDNGHGTHCAGDIASSGASSSGKYQGPAPEADLIGVKVLNKSGSGTLADIIEGVEWCI
CCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHH
QYNKEHTKNPIRIISMSLGGDALRYDKETDDPLVKAVEKAWNEGIVVCVAAGNSGPEAQT
HHCHHHCCCCEEEEEEECCCCCEECCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCCC
ISSPGVSEKVITVGAYDDNDTAGNEDDTVASFSSRGPTVYGKEKPDILAPGVDIVSLRSP
CCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHCCCCEEECCCCCCEECCCCCEEEECCC
RSYLDKLQKSNRVGSLYFSLSGTSMATPICAGIAALILQQNPQLSPDEVKTLIKQSPDQW
HHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCC
TNEDPNIYGAGAVNAENAVPKE
CCCCCCEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]