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The map label for this gene is hcaT [H]

Identifier: 154685735

GI number: 154685735

Start: 1263318

End: 1264502

Strand: Reverse

Name: hcaT [H]

Synonym: RBAM_013020

Alternate gene names: 154685735

Gene position: 1264502-1263318 (Counterclockwise)

Preceding gene: 154685737

Following gene: 154685734

Centisome position: 32.27

GC content: 45.32

Gene sequence:

>1185_bases
ATGAAAGCATATCGCTGGATGAGTCTGCATTTTTTTAGTTTTTTTATGACATGGGGTGTTTTTCTGCCCTATTGGACGGG
ATGGATGGTGCATATAAAAGGGGTTTCCGTATCCGAAGCCAGTTTCATTATGAGCCTGGGCTTGGCGGCAAGAGGGATTT
CCACATTATTATTCTTCCCTTATTTATCAGGAAAATTCAGCAGCAAAGCCTTATTAAACGGCGCCAGAATCGGGACGCTT
CTCGCACTTTTATGTTATATTCCCGCTGATTCTTTTCAAAGCCTGCTTCTTGTCACATTAGTGCTGCATGTGTTTTACCC
TGCTTTAATGCCTGCTTTAGACAGCGCTTGCGGCGTCCTTGTACAGCATAACGAATTAAAGGCCTATGGGACAAGCCGAC
AGTGGGGGTCCGCGGGTTTTGTTTCAGCCGGCGTCATCCTGTCCGTCTTTACAGGTATATTCGGAGATCAAGCGATTTTA
TGGGCACTGCTTTTGGGCACCCTGATATTCGCATGCCTCAGCTTTATGCATGCGCCAGCTGTTCTTTCAAAACGGCCGGA
AACAGACCGCGCAAGAAAAGAGGGCATGTTTGCCTTATTCCGCATCAAATATTTCGGTCTGGTGCTCGCAATCGTTATCT
TACTGCAGGCGGCACATGCATCTTACTACAATTACGGTTATCTCTTTTTGCAGGAAATCGATGCCCCCGCATATGCCATC
GGAATCATTATCAACATCGCCGTTATCGCGGAGATTATCTTCTTTGCAATCGCTGACCGATACTTTCATCAATTTACGGC
AGGCTCTTTGCTCGCCTTGGCGGCGCTTGGTGCTACCGTACGCTGGATACTCGTATTTGCATTTCCGCATGTCATCATTT
TCTGTATTGCGCAGACGCTGCATGCCTGTTCATTCGCGATGGGACACTATGCATTTATGAAATACTTAATTAAAAATATT
TCACCCGCGCAAATCCCGAAAGCCCAAGGCATATATTCAGCGCTTGCTCTCAGCTGGAGCACTGCGGTATTTACTATTTT
CGGAGGTTTTTTATATGAAATTGAACCCCGGTATGCCTTTATCGGAATGATTGTTTGTACGATCCCATCCATGCTACTCG
CCCTTTTATACAGAAAACTTGAAATGAAAAAGAGAGTTTTTGCAGGAAACGCACAAGATATATAG

Upstream 100 bases:

>100_bases
CGCCTTTATAAACTTCCATCGTCCACCCCAAAAATATGAGATATCTCATATTGTCTTTATGTCTTTACATGATAACATGC
CAATTAAAGGAGGAACGGGA

Downstream 100 bases:

>100_bases
CCATCGCTTTATAGACGATCACATGTCCCGCTGTCATTTTGACCGATTTGCGAATGCTGTGCGATGTATTTTCTCTATAT
TTAAAGTATTTCCTTCATAT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MKAYRWMSLHFFSFFMTWGVFLPYWTGWMVHIKGVSVSEASFIMSLGLAARGISTLLFFPYLSGKFSSKALLNGARIGTL
LALLCYIPADSFQSLLLVTLVLHVFYPALMPALDSACGVLVQHNELKAYGTSRQWGSAGFVSAGVILSVFTGIFGDQAIL
WALLLGTLIFACLSFMHAPAVLSKRPETDRARKEGMFALFRIKYFGLVLAIVILLQAAHASYYNYGYLFLQEIDAPAYAI
GIIINIAVIAEIIFFAIADRYFHQFTAGSLLALAALGATVRWILVFAFPHVIIFCIAQTLHACSFAMGHYAFMKYLIKNI
SPAQIPKAQGIYSALALSWSTAVFTIFGGFLYEIEPRYAFIGMIVCTIPSMLLALLYRKLEMKKRVFAGNAQDI

Sequences:

>Translated_394_residues
MKAYRWMSLHFFSFFMTWGVFLPYWTGWMVHIKGVSVSEASFIMSLGLAARGISTLLFFPYLSGKFSSKALLNGARIGTL
LALLCYIPADSFQSLLLVTLVLHVFYPALMPALDSACGVLVQHNELKAYGTSRQWGSAGFVSAGVILSVFTGIFGDQAIL
WALLLGTLIFACLSFMHAPAVLSKRPETDRARKEGMFALFRIKYFGLVLAIVILLQAAHASYYNYGYLFLQEIDAPAYAI
GIIINIAVIAEIIFFAIADRYFHQFTAGSLLALAALGATVRWILVFAFPHVIIFCIAQTLHACSFAMGHYAFMKYLIKNI
SPAQIPKAQGIYSALALSWSTAVFTIFGGFLYEIEPRYAFIGMIVCTIPSMLLALLYRKLEMKKRVFAGNAQDI
>Mature_394_residues
MKAYRWMSLHFFSFFMTWGVFLPYWTGWMVHIKGVSVSEASFIMSLGLAARGISTLLFFPYLSGKFSSKALLNGARIGTL
LALLCYIPADSFQSLLLVTLVLHVFYPALMPALDSACGVLVQHNELKAYGTSRQWGSAGFVSAGVILSVFTGIFGDQAIL
WALLLGTLIFACLSFMHAPAVLSKRPETDRARKEGMFALFRIKYFGLVLAIVILLQAAHASYYNYGYLFLQEIDAPAYAI
GIIINIAVIAEIIFFAIADRYFHQFTAGSLLALAALGATVRWILVFAFPHVIIFCIAQTLHACSFAMGHYAFMKYLIKNI
SPAQIPKAQGIYSALALSWSTAVFTIFGGFLYEIEPRYAFIGMIVCTIPSMLLALLYRKLEMKKRVFAGNAQDI

Specific function: Probable permease involved in the uptake of 3- phenylpropionic acid [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lacY/rafB permease family. HcaT subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788886, Length=378, Percent_Identity=29.1005291005291, Blast_Score=148, Evalue=6e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022814
- InterPro:   IPR016196
- InterPro:   IPR005276 [H]

Pfam domain/function: PF01306 LacY_symp [H]

EC number: NA

Molecular weight: Translated: 43687; Mature: 43687

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAYRWMSLHFFSFFMTWGVFLPYWTGWMVHIKGVSVSEASFIMSLGLAARGISTLLFFP
CCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
YLSGKFSSKALLNGARIGTLLALLCYIPADSFQSLLLVTLVLHVFYPALMPALDSACGVL
HHCCCCCCHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VQHNELKAYGTSRQWGSAGFVSAGVILSVFTGIFGDQAILWALLLGTLIFACLSFMHAPA
EECCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
VLSKRPETDRARKEGMFALFRIKYFGLVLAIVILLQAAHASYYNYGYLFLQEIDAPAYAI
HHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEECCCCHHHH
GIIINIAVIAEIIFFAIADRYFHQFTAGSLLALAALGATVRWILVFAFPHVIIFCIAQTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HACSFAMGHYAFMKYLIKNISPAQIPKAQGIYSALALSWSTAVFTIFGGFLYEIEPRYAF
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHH
IGMIVCTIPSMLLALLYRKLEMKKRVFAGNAQDI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKAYRWMSLHFFSFFMTWGVFLPYWTGWMVHIKGVSVSEASFIMSLGLAARGISTLLFFP
CCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
YLSGKFSSKALLNGARIGTLLALLCYIPADSFQSLLLVTLVLHVFYPALMPALDSACGVL
HHCCCCCCHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VQHNELKAYGTSRQWGSAGFVSAGVILSVFTGIFGDQAILWALLLGTLIFACLSFMHAPA
EECCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
VLSKRPETDRARKEGMFALFRIKYFGLVLAIVILLQAAHASYYNYGYLFLQEIDAPAYAI
HHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEECCCCHHHH
GIIINIAVIAEIIFFAIADRYFHQFTAGSLLALAALGATVRWILVFAFPHVIIFCIAQTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HACSFAMGHYAFMKYLIKNISPAQIPKAQGIYSALALSWSTAVFTIFGGFLYEIEPRYAF
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHH
IGMIVCTIPSMLLALLYRKLEMKKRVFAGNAQDI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9603882; 9205837; 9278503 [H]