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The map label for this gene is blt [H]

Identifier: 154685509

GI number: 154685509

Start: 1052259

End: 1053455

Strand: Reverse

Name: blt [H]

Synonym: RBAM_010750

Alternate gene names: 154685509

Gene position: 1053455-1052259 (Counterclockwise)

Preceding gene: 154685510

Following gene: 154685508

Centisome position: 26.88

GC content: 46.53

Gene sequence:

>1197_bases
ATGCAAAAACCGATCAGAGAACAAAAGGCTGTACTCATCATCTTATTAAGCAATATATTTATTGCGTTTTTAGGCATCGG
ATTGATCATTCCCGTTATGCCGCTGTTTATGAATGTGATGCACTTGACGGGCAGCACAATGGGATACCTTGTCGCTGCCT
TCGCCGTTGCGCAGTTAATTGCATCCCCCATTGCGGGGAGATGGGTTGACCGCTTCGGCAGAAAAATCATGATTCTCGCC
GGACTGTTTTTGTTCGCATTATCCGAACTGACCTTCGGATTGGGCACACATGTCTCCATTTTATATTTCGCAAGGGTGCT
GGGGGGCATCAGTGCCGCTTTTATCATGCCCGCTGTTACAGCGTATGTCGCAGATATTACGACGGTGCAGGAAAGATCGA
AGGCGATGGGCTATGTATCTGCCGCCATCAGCACAGGGTTTATCATCGGGCCCGGCATAGGAGGATTTATTGCCGATCAC
GGCGTTCGTATGCCGTTTTTCTTTGCGGCGGGAATTGCATTTATCGCAGTGATTTCCTCTGTCTTTATGCTGAAAGAGCC
GTTAACTAAAGAAGAACGCGCAAAGCAGATGGAAAGCGTCAAAGAATCAACTTTCTTAAAAGATTTGAAAAAGTCGATTC
ATCCAAACTACTTGATCGCCTTTATCATCGTTTTTGTTTTGGCATTCGGACTGTCGGCGTACGAAACGGTATTCAGCTTA
TTTACAAACCATAAATTCGGCTTCACGCCAAAGGATATCGCCATTATTATTACGTTCAGCTCCATTGTGGCGGTCCTCAT
TCAAGTGCTCGCGTTCGGAAGACTTGTCAACTTCCTCGGGGAGAAAAAAGTCATTCAGCTTTGTCTGATCATCGGCGCTG
TGCTGGCTTTCGTTTCTACGGTGATGTCAGGATTTTTGCCCGTGCTCGCTGTCACCTGCATCATTTTCCTTGCGTTTGAT
TTACTGCGCCCGGCACTGACTACTTATTTATCGAAAATCGCAGGAAATCAGCAGGGGTTTGTTGCCGGTATGAACTCAAC
ATACACGAGTTTGGGCACTATTTTCGGCCCGGCTCTGGGCGGTATTTTATTTGATATGAATATTCACTTCCCGTTTCTAT
TCGCAGGTGTGGTTTTATTTCTCGGTCTGGGATTGACCTTTGTGTGGAAAGGAAAAGCAGGCGAGGTTTCGGAGTAG

Upstream 100 bases:

>100_bases
TCAGCAAAGAATCGAATTGCAAAAAGAAGCCATCTCCGTGTTTATTCACGGGTCGATCAGCGCTGATAAACAGAATGACA
GCTGACAAAGGAGAAACAAC

Downstream 100 bases:

>100_bases
GGTGCACTCACCCATCTCCGGAGCTTTTTTTGCGTTTTCGATAAGAAAAACGAATGGATTATGAGAAATAATTCATTATT
TATTTATCTCAAAATCTATA

Product: major facilitator transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter 2 [H]

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MQKPIREQKAVLIILLSNIFIAFLGIGLIIPVMPLFMNVMHLTGSTMGYLVAAFAVAQLIASPIAGRWVDRFGRKIMILA
GLFLFALSELTFGLGTHVSILYFARVLGGISAAFIMPAVTAYVADITTVQERSKAMGYVSAAISTGFIIGPGIGGFIADH
GVRMPFFFAAGIAFIAVISSVFMLKEPLTKEERAKQMESVKESTFLKDLKKSIHPNYLIAFIIVFVLAFGLSAYETVFSL
FTNHKFGFTPKDIAIIITFSSIVAVLIQVLAFGRLVNFLGEKKVIQLCLIIGAVLAFVSTVMSGFLPVLAVTCIIFLAFD
LLRPALTTYLSKIAGNQQGFVAGMNSTYTSLGTIFGPALGGILFDMNIHFPFLFAGVVLFLGLGLTFVWKGKAGEVSE

Sequences:

>Translated_398_residues
MQKPIREQKAVLIILLSNIFIAFLGIGLIIPVMPLFMNVMHLTGSTMGYLVAAFAVAQLIASPIAGRWVDRFGRKIMILA
GLFLFALSELTFGLGTHVSILYFARVLGGISAAFIMPAVTAYVADITTVQERSKAMGYVSAAISTGFIIGPGIGGFIADH
GVRMPFFFAAGIAFIAVISSVFMLKEPLTKEERAKQMESVKESTFLKDLKKSIHPNYLIAFIIVFVLAFGLSAYETVFSL
FTNHKFGFTPKDIAIIITFSSIVAVLIQVLAFGRLVNFLGEKKVIQLCLIIGAVLAFVSTVMSGFLPVLAVTCIIFLAFD
LLRPALTTYLSKIAGNQQGFVAGMNSTYTSLGTIFGPALGGILFDMNIHFPFLFAGVVLFLGLGLTFVWKGKAGEVSE
>Mature_398_residues
MQKPIREQKAVLIILLSNIFIAFLGIGLIIPVMPLFMNVMHLTGSTMGYLVAAFAVAQLIASPIAGRWVDRFGRKIMILA
GLFLFALSELTFGLGTHVSILYFARVLGGISAAFIMPAVTAYVADITTVQERSKAMGYVSAAISTGFIIGPGIGGFIADH
GVRMPFFFAAGIAFIAVISSVFMLKEPLTKEERAKQMESVKESTFLKDLKKSIHPNYLIAFIIVFVLAFGLSAYETVFSL
FTNHKFGFTPKDIAIIITFSSIVAVLIQVLAFGRLVNFLGEKKVIQLCLIIGAVLAFVSTVMSGFLPVLAVTCIIFLAFD
LLRPALTTYLSKIAGNQQGFVAGMNSTYTSLGTIFGPALGGILFDMNIHFPFLFAGVVLFLGLGLTFVWKGKAGEVSE

Specific function: Energy-dependent efflux pump responsible for decreased drug accumulation in multi-drug-resistant cells. Probably uses a transmembrane proton gradient as the energy source. Causes the efflux of a variety of toxic substances, including such structurally div

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family [H]

Homologues:

Organism=Homo sapiens, GI217416366, Length=394, Percent_Identity=23.6040609137056, Blast_Score=83, Evalue=6e-16,
Organism=Homo sapiens, GI39753965, Length=319, Percent_Identity=26.6457680250784, Blast_Score=69, Evalue=7e-12,
Organism=Escherichia coli, GI1787291, Length=385, Percent_Identity=25.7142857142857, Blast_Score=79, Evalue=6e-16,
Organism=Drosophila melanogaster, GI17738085, Length=354, Percent_Identity=27.9661016949153, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI17137160, Length=232, Percent_Identity=25.4310344827586, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI221513297, Length=366, Percent_Identity=24.3169398907104, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21357569, Length=371, Percent_Identity=24.7978436657682, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI62471713, Length=270, Percent_Identity=29.6296296296296, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI62471717, Length=270, Percent_Identity=29.6296296296296, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI62471715, Length=270, Percent_Identity=29.6296296296296, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24656463, Length=371, Percent_Identity=24.7978436657682, Blast_Score=65, Evalue=7e-11,
Organism=Drosophila melanogaster, GI24656468, Length=371, Percent_Identity=24.7978436657682, Blast_Score=65, Evalue=7e-11,
Organism=Drosophila melanogaster, GI45550550, Length=297, Percent_Identity=26.5993265993266, Blast_Score=65, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734
- InterPro:   IPR005829
- InterPro:   IPR001958 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42993; Mature: 42993

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHEECCCCHHHHHHHHHHHHHCCHHEEECCCCCCCCC
>Mature Secondary Structure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HHHEECCCCHHHHHHHHHHHHHCCHHEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7608059; 8969508; 9384377 [H]