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The map label for this gene is yhfT [H]

Identifier: 154685488

GI number: 154685488

Start: 1034671

End: 1036107

Strand: Reverse

Name: yhfT [H]

Synonym: RBAM_010540

Alternate gene names: 154685488

Gene position: 1036107-1034671 (Counterclockwise)

Preceding gene: 154685489

Following gene: 154685487

Centisome position: 26.44

GC content: 53.51

Gene sequence:

>1437_bases
ATGATTACATCATCATATTCAGATACAGCCGGACAAGCGCCGGAACAGATCGCAATTCAGACAGAAACAGAACAGATCAC
CTATCAAACTTGGAACAGGCTGGTTAACCAAACCGCAAACTGGCTTGGCAGCCTTTCAGGTAAGCCGGAAAACATTGCAA
TATTACTGCCGAACGGCATCCCATTTTTACAACTTTTCGCAGGGGCGGCGCATGCCGGCTGCACGGCGGTTCCGTTAGAT
CCCCGCTCGGCAGCGGCGGAACTCGCGGACCAGCTGCACATCAGCAAAACCGGCCTCGTCATCTGTGACCGCCGGCTGAG
CGGCAGACTGGACGGCGTCAAAACGCCTGTCATCCTGTGGGATGAATGCAGGCCTTTGATCGAGGCGGAAAAAACGGAAG
GCGCTTTTATCAAAAACGCCGGCCTGCCCTTTTATACCGGATTCACCTCGGGATCGACCGGTAAGCCGAAAGCCTTCACC
CGCTCTCACCGTTCGTGGCTGGAGAGCTTTTCTTGTACGAAACGTGAATTCGGCATTTCCCCTGATGACAAAGTGCTGGT
ACCGGGCACGCTTCGCTCGTCACACTTTTTATACGGCGCCGTCAGCACTTTGTTTATCGGCGGAACCGTCATCCTTCAAA
GAAAATTCAACCCGGCCGGAATGAAAGACCGGCTCAGACAAGCTGACGTTCTATATACCGTTCCGACAATGACGGACGCC
CTGCTCTCACTAGAGGCGTTTCCGGATACACCGCTCAAGGTGATTTCTTCCGGTGCAGACTGGCCCGCTTCGTCTAAACA
AGCGCTGTCAGAGCGCTATCCCCATCTGCGGTTGTACGATTTTTACGGAACGTCCGAGCTGAGTTTCGTGGCGTTCTTAT
CGCCTGAAGACAGCGCCCGAAAGCCTGACGCGGCGGGCCGCCCGTTTGAAAATGTTACAATCGAAATCCGCCGGCCCGGC
GGAGAACGCTGCATGCCGGGCGAAACGGGGAAGATTTTTGTGAACAGCCCGATGAAATTTTCCGGATACATCAACGGCGC
AAAACCTGATGATTCCGGCTGGATGACGGTTGATGACATGGGATATATCGATCGTGAAGGCTATCTTTATATCGAAGGAC
GTGAAAACGGGATGATCGTATCAGGCGGCCTGAATGTATTCCCAGAAGAGATAGAACGTGTGCTCAACCGCTGTCCGGGC
GTCAGAAAAGCGGCCGTCATCGGCATTCCTGATGATTATTGGGGCAGCATCGCCGTTGCGGTGATCGACGGACATGCAAA
TGTAAAGGAACTGAAGAAACAGTGCCGCGAGCAGCTGGCGGCATATAAAATCCCGAAAAAATGGCTGTTTTCAGAAATAC
CGGAAACAGCCGGCGGAAAGATCGCCCGTGCCGCCGTACAAAAGCAGGCAAAGGACATGATGGAATGGAAGCTGTAA

Upstream 100 bases:

>100_bases
TGCTTAAAGCGGCCGCAGCCTCATTCGCAGCCGTAAGAATCAGGAAAACTCTTCAAGCAAGCCGGCTTTTGCCGACGAAA
GGAAGCTGATTTTAGTCATC

Downstream 100 bases:

>100_bases
TTACGGATGCGAAACGGACAATTTTCGCCCGGGAAAACGGCCTGTTAAAAGACTGTCTCCCAGAAGATTTGGCAGCTCCG
CTGATCCGCCATTTGAGCCG

Product: acyl-CoA synthetase

Products: AMP; diphosphate; acyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MITSSYSDTAGQAPEQIAIQTETEQITYQTWNRLVNQTANWLGSLSGKPENIAILLPNGIPFLQLFAGAAHAGCTAVPLD
PRSAAAELADQLHISKTGLVICDRRLSGRLDGVKTPVILWDECRPLIEAEKTEGAFIKNAGLPFYTGFTSGSTGKPKAFT
RSHRSWLESFSCTKREFGISPDDKVLVPGTLRSSHFLYGAVSTLFIGGTVILQRKFNPAGMKDRLRQADVLYTVPTMTDA
LLSLEAFPDTPLKVISSGADWPASSKQALSERYPHLRLYDFYGTSELSFVAFLSPEDSARKPDAAGRPFENVTIEIRRPG
GERCMPGETGKIFVNSPMKFSGYINGAKPDDSGWMTVDDMGYIDREGYLYIEGRENGMIVSGGLNVFPEEIERVLNRCPG
VRKAAVIGIPDDYWGSIAVAVIDGHANVKELKKQCREQLAAYKIPKKWLFSEIPETAGGKIARAAVQKQAKDMMEWKL

Sequences:

>Translated_478_residues
MITSSYSDTAGQAPEQIAIQTETEQITYQTWNRLVNQTANWLGSLSGKPENIAILLPNGIPFLQLFAGAAHAGCTAVPLD
PRSAAAELADQLHISKTGLVICDRRLSGRLDGVKTPVILWDECRPLIEAEKTEGAFIKNAGLPFYTGFTSGSTGKPKAFT
RSHRSWLESFSCTKREFGISPDDKVLVPGTLRSSHFLYGAVSTLFIGGTVILQRKFNPAGMKDRLRQADVLYTVPTMTDA
LLSLEAFPDTPLKVISSGADWPASSKQALSERYPHLRLYDFYGTSELSFVAFLSPEDSARKPDAAGRPFENVTIEIRRPG
GERCMPGETGKIFVNSPMKFSGYINGAKPDDSGWMTVDDMGYIDREGYLYIEGRENGMIVSGGLNVFPEEIERVLNRCPG
VRKAAVIGIPDDYWGSIAVAVIDGHANVKELKKQCREQLAAYKIPKKWLFSEIPETAGGKIARAAVQKQAKDMMEWKL
>Mature_478_residues
MITSSYSDTAGQAPEQIAIQTETEQITYQTWNRLVNQTANWLGSLSGKPENIAILLPNGIPFLQLFAGAAHAGCTAVPLD
PRSAAAELADQLHISKTGLVICDRRLSGRLDGVKTPVILWDECRPLIEAEKTEGAFIKNAGLPFYTGFTSGSTGKPKAFT
RSHRSWLESFSCTKREFGISPDDKVLVPGTLRSSHFLYGAVSTLFIGGTVILQRKFNPAGMKDRLRQADVLYTVPTMTDA
LLSLEAFPDTPLKVISSGADWPASSKQALSERYPHLRLYDFYGTSELSFVAFLSPEDSARKPDAAGRPFENVTIEIRRPG
GERCMPGETGKIFVNSPMKFSGYINGAKPDDSGWMTVDDMGYIDREGYLYIEGRENGMIVSGGLNVFPEEIERVLNRCPG
VRKAAVIGIPDDYWGSIAVAVIDGHANVKELKKQCREQLAAYKIPKKWLFSEIPETAGGKIARAAVQKQAKDMMEWKL

Specific function: May be involved in fatty acid metabolism [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI187761345, Length=509, Percent_Identity=24.3614931237721, Blast_Score=105, Evalue=9e-23,
Organism=Homo sapiens, GI187761343, Length=509, Percent_Identity=24.3614931237721, Blast_Score=105, Evalue=9e-23,
Organism=Homo sapiens, GI156151445, Length=359, Percent_Identity=27.5766016713092, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI38505220, Length=493, Percent_Identity=23.3265720081136, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI115511026, Length=504, Percent_Identity=23.8095238095238, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI42544132, Length=496, Percent_Identity=22.9838709677419, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI122937307, Length=501, Percent_Identity=22.1556886227545, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI157311624, Length=496, Percent_Identity=23.1854838709677, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI157311622, Length=496, Percent_Identity=23.1854838709677, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI58082049, Length=496, Percent_Identity=23.5887096774194, Blast_Score=80, Evalue=4e-15,
Organism=Escherichia coli, GI1788107, Length=538, Percent_Identity=23.4200743494424, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI145693145, Length=508, Percent_Identity=24.0157480314961, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1786810, Length=349, Percent_Identity=26.0744985673352, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI221142682, Length=490, Percent_Identity=24.6938775510204, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1788595, Length=330, Percent_Identity=25.1515151515152, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17558820, Length=449, Percent_Identity=26.9487750556793, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI32563687, Length=508, Percent_Identity=24.0157480314961, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17531443, Length=518, Percent_Identity=24.9034749034749, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17538037, Length=523, Percent_Identity=26.9598470363289, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17560308, Length=358, Percent_Identity=26.2569832402235, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17557194, Length=518, Percent_Identity=25.8687258687259, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI71994703, Length=353, Percent_Identity=24.3626062322946, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71994690, Length=353, Percent_Identity=24.3626062322946, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71994694, Length=353, Percent_Identity=24.3626062322946, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71996755, Length=484, Percent_Identity=24.1735537190083, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17559526, Length=351, Percent_Identity=27.0655270655271, Blast_Score=93, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI71985884, Length=369, Percent_Identity=24.6612466124661, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI25141359, Length=464, Percent_Identity=24.3534482758621, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71983001, Length=457, Percent_Identity=25.382932166302, Blast_Score=68, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71982997, Length=457, Percent_Identity=25.382932166302, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319699, Length=527, Percent_Identity=24.8576850094877, Blast_Score=118, Evalue=3e-27,
Organism=Drosophila melanogaster, GI18859661, Length=479, Percent_Identity=25.678496868476, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24656500, Length=478, Percent_Identity=26.1506276150628, Blast_Score=99, Evalue=9e-21,
Organism=Drosophila melanogaster, GI21356947, Length=346, Percent_Identity=24.5664739884393, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24581924, Length=365, Percent_Identity=25.4794520547945, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24648260, Length=345, Percent_Identity=26.0869565217391, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24648257, Length=446, Percent_Identity=25.1121076233184, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24648255, Length=336, Percent_Identity=23.5119047619048, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI19922652, Length=282, Percent_Identity=26.241134751773, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24648253, Length=336, Percent_Identity=24.702380952381, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI21358303, Length=355, Percent_Identity=25.3521126760563, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24653035, Length=464, Percent_Identity=23.0603448275862, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI281365686, Length=172, Percent_Identity=28.4883720930233, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI21356441, Length=287, Percent_Identity=22.9965156794425, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI161076582, Length=412, Percent_Identity=23.7864077669903, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI21355181, Length=460, Percent_Identity=23.695652173913, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI20130357, Length=343, Percent_Identity=24.198250728863, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24667959, Length=370, Percent_Identity=24.5945945945946, Blast_Score=66, Evalue=5e-11,
Organism=Drosophila melanogaster, GI62472339, Length=370, Percent_Identity=24.8648648648649, Blast_Score=66, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24667955, Length=370, Percent_Identity=24.8648648648649, Blast_Score=66, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3

Molecular weight: Translated: 52525; Mature: 52525

Theoretical pI: Translated: 7.43; Mature: 7.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITSSYSDTAGQAPEQIAIQTETEQITYQTWNRLVNQTANWLGSLSGKPENIAILLPNGI
CCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCC
PFLQLFAGAAHAGCTAVPLDPRSAAAELADQLHISKTGLVICDRRLSGRLDGVKTPVILW
HHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHEECCCCEEEECCHHCCCCCCCCCCEEEE
DECRPLIEAEKTEGAFIKNAGLPFYTGFTSGSTGKPKAFTRSHRSWLESFSCTKREFGIS
CCCCCHHHCCCCCCCEEECCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHCCCHHHCCCC
PDDKVLVPGTLRSSHFLYGAVSTLFIGGTVILQRKFNPAGMKDRLRQADVLYTVPTMTDA
CCCCEEECCCCCCCCCHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHCCEEEECCHHHHH
LLSLEAFPDTPLKVISSGADWPASSKQALSERYPHLRLYDFYGTSELSFVAFLSPEDSAR
HHHHHCCCCCHHHHHCCCCCCCCCHHHHHHHCCCCEEEEEEECCCCEEEEEEECCCCCCC
KPDAAGRPFENVTIEIRRPGGERCMPGETGKIFVNSPMKFSGYINGAKPDDSGWMTVDDM
CCCCCCCCCCCEEEEEECCCCCCCCCCCCCEEEECCCEEEEEEECCCCCCCCCCEEECCC
GYIDREGYLYIEGRENGMIVSGGLNVFPEEIERVLNRCPGVRKAAVIGIPDDYWGSIAVA
CCCCCCCEEEEEECCCCEEEECCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEE
VIDGHANVKELKKQCREQLAAYKIPKKWLFSEIPETAGGKIARAAVQKQAKDMMEWKL
EEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MITSSYSDTAGQAPEQIAIQTETEQITYQTWNRLVNQTANWLGSLSGKPENIAILLPNGI
CCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCC
PFLQLFAGAAHAGCTAVPLDPRSAAAELADQLHISKTGLVICDRRLSGRLDGVKTPVILW
HHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHEECCCCEEEECCHHCCCCCCCCCCEEEE
DECRPLIEAEKTEGAFIKNAGLPFYTGFTSGSTGKPKAFTRSHRSWLESFSCTKREFGIS
CCCCCHHHCCCCCCCEEECCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHCCCHHHCCCC
PDDKVLVPGTLRSSHFLYGAVSTLFIGGTVILQRKFNPAGMKDRLRQADVLYTVPTMTDA
CCCCEEECCCCCCCCCHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHCCEEEECCHHHHH
LLSLEAFPDTPLKVISSGADWPASSKQALSERYPHLRLYDFYGTSELSFVAFLSPEDSAR
HHHHHCCCCCHHHHHCCCCCCCCCHHHHHHHCCCCEEEEEEECCCCEEEEEEECCCCCCC
KPDAAGRPFENVTIEIRRPGGERCMPGETGKIFVNSPMKFSGYINGAKPDDSGWMTVDDM
CCCCCCCCCCCEEEEEECCCCCCCCCCCCCEEEECCCEEEEEEECCCCCCCCCCEEECCC
GYIDREGYLYIEGRENGMIVSGGLNVFPEEIERVLNRCPGVRKAAVIGIPDDYWGSIAVA
CCCCCCCEEEEEECCCCEEEECCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEE
VIDGHANVKELKKQCREQLAAYKIPKKWLFSEIPETAGGKIARAAVQKQAKDMMEWKL
EEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; long-chain carboxylic acid; CoA

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]