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The map label for this gene is yhfS [H]

Identifier: 154685487

GI number: 154685487

Start: 1033592

End: 1034683

Strand: Reverse

Name: yhfS [H]

Synonym: RBAM_010530

Alternate gene names: 154685487

Gene position: 1034683-1033592 (Counterclockwise)

Preceding gene: 154685488

Following gene: 154685486

Centisome position: 26.4

GC content: 56.87

Gene sequence:

>1092_bases
ATGGAAGCTGTAATTACGGATGCGAAACGGACAATTTTCGCCCGGGAAAACGGCCTGTTAAAAGACTGTCTCCCAGAAGA
TTTGGCAGCTCCGCTGATCCGCCATTTGAGCCGGAACATGCCCGAAGACATTGATGAAGTCCTGATCGGAAACGCGACGG
GCCGCGGCGGGAATCTCGCCCGGCTGTCAGCCTTGGCCGCCGGACTTCCTTTGAGCGTGTCCGGTACGACCATCGACAGG
CAGTGCGGCTCCGGACTGGACGCCGTGAGGGTCGCGTGCCACTTTGTCAAAGCCGGCGCGGGGAGAACGTACATCGCGGG
AGGAGCGGAGAGCAGCAGCCGTTCCCCTTTCTCATCCCGGGCGCGCTTTTCTCCTGAACACATCGGCGATCCTGATATGG
GCATAGCAGCAGAGTATACGGCACAGGCTTACGGCGTAACGAGAAAGATGCAGGACGAATATGCCCTTCTCAGCTATGAA
CGGAGCATCAAAGCGCACGAAGACGGCTTGTACCGCCCGGAAATTCTTCCCTGTCACGGATTCGCCGCTGACGAAAACAT
GCTGAAAAAGCGGAACATGGAGCCTCTCATCTCCCGTGCCAAGCCCGTCTTTCAGTCCGGAGGCTCTGTCACCGCCGCCA
ACAGCTGCGGAATTTCTGACGGGGCGGCGGCAGTTTGTGTGATGGAAGAACAAAAAGCGCGGTCTCTCGGTTTAAAGCCG
GGATTGCGTTTTATCGGAAGCGCGGTAAGCGGCGTCCATCCGCATTATCCGTCAGCAGCTCCGGTTTATGCCGTCAAAAA
GCTTTTGAAGGAACGGGACCTGTCACCGGATGATATTGATATATACGAAATCAACGAGGCGTTTGCCGTCAAAATATGCG
TTTTTTCACAGCAGCTTCGAGTGCCGTATTCTAAAATAAATGTCAGGGGCGGCGCTCTGGCCGTCGGGCATCCGTACGGG
GCTTCCGGGGCGGCGCTTGTCACCCGGCTTTTTTATGAAGCAAAAAGACGCCCGGATGCCCGCTACGCAGCAGCAGCGAT
CGGCAGCGGAGGCGGAGTCGGACTGGCGCTTCTCTTTGAAGTGTTAACGTAA

Upstream 100 bases:

>100_bases
ATAAAATCCCGAAAAAATGGCTGTTTTCAGAAATACCGGAAACAGCCGGCGGAAAGATCGCCCGTGCCGCCGTACAAAAG
CAGGCAAAGGACATGATGGA

Downstream 100 bases:

>100_bases
CGGCAGTATAATAGATGATATCGATAAAAAATGATCGGAGTGAAGACTTTATGACTGCCGTTTGTTTAGTAAGACATGGA
GAGACTGATTGGAATGCCCA

Product: acetyl-CoA acetyltransferase

Products: CoA; acetoacetyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MEAVITDAKRTIFARENGLLKDCLPEDLAAPLIRHLSRNMPEDIDEVLIGNATGRGGNLARLSALAAGLPLSVSGTTIDR
QCGSGLDAVRVACHFVKAGAGRTYIAGGAESSSRSPFSSRARFSPEHIGDPDMGIAAEYTAQAYGVTRKMQDEYALLSYE
RSIKAHEDGLYRPEILPCHGFAADENMLKKRNMEPLISRAKPVFQSGGSVTAANSCGISDGAAAVCVMEEQKARSLGLKP
GLRFIGSAVSGVHPHYPSAAPVYAVKKLLKERDLSPDDIDIYEINEAFAVKICVFSQQLRVPYSKINVRGGALAVGHPYG
ASGAALVTRLFYEAKRRPDARYAAAAIGSGGGVGLALLFEVLT

Sequences:

>Translated_363_residues
MEAVITDAKRTIFARENGLLKDCLPEDLAAPLIRHLSRNMPEDIDEVLIGNATGRGGNLARLSALAAGLPLSVSGTTIDR
QCGSGLDAVRVACHFVKAGAGRTYIAGGAESSSRSPFSSRARFSPEHIGDPDMGIAAEYTAQAYGVTRKMQDEYALLSYE
RSIKAHEDGLYRPEILPCHGFAADENMLKKRNMEPLISRAKPVFQSGGSVTAANSCGISDGAAAVCVMEEQKARSLGLKP
GLRFIGSAVSGVHPHYPSAAPVYAVKKLLKERDLSPDDIDIYEINEAFAVKICVFSQQLRVPYSKINVRGGALAVGHPYG
ASGAALVTRLFYEAKRRPDARYAAAAIGSGGGVGLALLFEVLT
>Mature_363_residues
MEAVITDAKRTIFARENGLLKDCLPEDLAAPLIRHLSRNMPEDIDEVLIGNATGRGGNLARLSALAAGLPLSVSGTTIDR
QCGSGLDAVRVACHFVKAGAGRTYIAGGAESSSRSPFSSRARFSPEHIGDPDMGIAAEYTAQAYGVTRKMQDEYALLSYE
RSIKAHEDGLYRPEILPCHGFAADENMLKKRNMEPLISRAKPVFQSGGSVTAANSCGISDGAAAVCVMEEQKARSLGLKP
GLRFIGSAVSGVHPHYPSAAPVYAVKKLLKERDLSPDDIDIYEINEAFAVKICVFSQQLRVPYSKINVRGGALAVGHPYG
ASGAALVTRLFYEAKRRPDARYAAAAIGSGGGVGLALLFEVLT

Specific function: May be involved in fatty acid metabolism [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI148539872, Length=389, Percent_Identity=33.9331619537275, Blast_Score=193, Evalue=3e-49,
Organism=Homo sapiens, GI4501853, Length=386, Percent_Identity=33.419689119171, Blast_Score=188, Evalue=5e-48,
Organism=Homo sapiens, GI167614485, Length=389, Percent_Identity=32.6478149100257, Blast_Score=188, Evalue=9e-48,
Organism=Homo sapiens, GI4557237, Length=347, Percent_Identity=34.0057636887608, Blast_Score=171, Evalue=8e-43,
Organism=Homo sapiens, GI4504327, Length=422, Percent_Identity=29.1469194312796, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI194353979, Length=367, Percent_Identity=28.3378746594005, Blast_Score=129, Evalue=4e-30,
Organism=Escherichia coli, GI1787663, Length=399, Percent_Identity=38.3458646616541, Blast_Score=212, Evalue=3e-56,
Organism=Escherichia coli, GI1788554, Length=393, Percent_Identity=36.3867684478371, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI87082165, Length=344, Percent_Identity=37.2093023255814, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI48994986, Length=385, Percent_Identity=35.5844155844156, Blast_Score=192, Evalue=4e-50,
Organism=Escherichia coli, GI1788683, Length=414, Percent_Identity=27.536231884058, Blast_Score=125, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI133906874, Length=390, Percent_Identity=36.6666666666667, Blast_Score=202, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI25147385, Length=389, Percent_Identity=33.1619537275064, Blast_Score=172, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17535921, Length=348, Percent_Identity=33.9080459770115, Blast_Score=168, Evalue=5e-42,
Organism=Caenorhabditis elegans, GI17551802, Length=421, Percent_Identity=27.790973871734, Blast_Score=134, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI17535917, Length=381, Percent_Identity=27.8215223097113, Blast_Score=109, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6325229, Length=391, Percent_Identity=31.9693094629156, Blast_Score=162, Evalue=6e-41,
Organism=Saccharomyces cerevisiae, GI6322031, Length=387, Percent_Identity=32.0413436692506, Blast_Score=156, Evalue=4e-39,
Organism=Drosophila melanogaster, GI24655093, Length=392, Percent_Identity=35.4591836734694, Blast_Score=216, Evalue=1e-56,
Organism=Drosophila melanogaster, GI17648125, Length=391, Percent_Identity=31.9693094629156, Blast_Score=175, Evalue=4e-44,
Organism=Drosophila melanogaster, GI24640423, Length=353, Percent_Identity=32.8611898016997, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI17137578, Length=425, Percent_Identity=29.4117647058824, Blast_Score=130, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: 2.3.1.9

Molecular weight: Translated: 38595; Mature: 38595

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: PS00737 THIOLASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEAVITDAKRTIFARENGLLKDCLPEDLAAPLIRHLSRNMPEDIDEVLIGNATGRGGNLA
CCCCHHHHHHHEEECCCCCHHHCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCHH
RLSALAAGLPLSVSGTTIDRQCGSGLDAVRVACHFVKAGAGRTYIAGGAESSSRSPFSSR
HHHHHHCCCCEEECCCEEHHHHCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHH
ARFSPEHIGDPDMGIAAEYTAQAYGVTRKMQDEYALLSYERSIKAHEDGLYRPEILPCHG
CCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCC
FAADENMLKKRNMEPLISRAKPVFQSGGSVTAANSCGISDGAAAVCVMEEQKARSLGLKP
CCCCHHHHHHCCCHHHHHHCCHHHHCCCCEEECCCCCCCCCCEEEEEECHHHHHHCCCCC
GLRFIGSAVSGVHPHYPSAAPVYAVKKLLKERDLSPDDIDIYEINEAFAVKICVFSQQLR
CHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCEEEEEEEEHHHHC
VPYSKINVRGGALAVGHPYGASGAALVTRLFYEAKRRPDARYAAAAIGSGGGVGLALLFE
CCHHHEEECCCEEEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHH
VLT
HHC
>Mature Secondary Structure
MEAVITDAKRTIFARENGLLKDCLPEDLAAPLIRHLSRNMPEDIDEVLIGNATGRGGNLA
CCCCHHHHHHHEEECCCCCHHHCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCHH
RLSALAAGLPLSVSGTTIDRQCGSGLDAVRVACHFVKAGAGRTYIAGGAESSSRSPFSSR
HHHHHHCCCCEEECCCEEHHHHCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHH
ARFSPEHIGDPDMGIAAEYTAQAYGVTRKMQDEYALLSYERSIKAHEDGLYRPEILPCHG
CCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCC
FAADENMLKKRNMEPLISRAKPVFQSGGSVTAANSCGISDGAAAVCVMEEQKARSLGLKP
CCCCHHHHHHCCCHHHHHHCCHHHHCCCCEEECCCCCCCCCCEEEEEECHHHHHHCCCCC
GLRFIGSAVSGVHPHYPSAAPVYAVKKLLKERDLSPDDIDIYEINEAFAVKICVFSQQLR
CHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCEEEEEEEEHHHHC
VPYSKINVRGGALAVGHPYGASGAALVTRLFYEAKRRPDARYAAAAIGSGGGVGLALLFE
CCHHHEEECCCEEEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHH
VLT
HHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: acetyl-CoA

Specific reaction: 2 acetyl-CoA = CoA + acetoacetyl-CoA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]