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The map label for this gene is yfiU [H]

Identifier: 154685305

GI number: 154685305

Start: 845899

End: 847491

Strand: Reverse

Name: yfiU [H]

Synonym: RBAM_008510

Alternate gene names: 154685305

Gene position: 847491-845899 (Counterclockwise)

Preceding gene: 154685306

Following gene: 154685299

Centisome position: 21.63

GC content: 53.92

Gene sequence:

>1593_bases
ATGCCGGAGCCATTGCAAACAGAAAAAAACCAGAGCCGGGCCATCACTCTCTTTACGATCGGTGTGTTCATGGCGGCGCT
GGACAACGGAATTATTTCCGCTGCTCTGACGACGATTAACAGCTCTTTTGATGTTTCTCCTTCATGGGGATCGTGGGGAG
TGACCCTTTACACATTGGGACTCAGTGTCAGTGTGCCGATTGTCGGCAAGCTGTCGGACCGATACGGGCGGAAAAAGCTG
TTTATGATTGAAGTCGGGCTGTTCGGACTCGGCTCTTTGCTTGTCGCACTCAGTCAGAGCTTCCCCATGTTTTTGGCGGC
ACGGCTCATTCAGGCGCTTGGCGGCGGCGGAATTTTTATTATCGGGAGTTCGCATGTGCTGGCCGTGCTTCCGAAGGAGA
AACAAGGGAAAGCTCTCGGGCTTTTAGGGGCGATGAACGGTATGGCGGCCGTCCTCGGACCGAATATCGGGAGCTTTTTG
CTTGATGTAACCGGGTCGTGGCATTGGCTGTTCTTGATTAACCTGCCGATTGCCGCCGCTTTGGTTGTGTGCGGCGGTTT
GTTTATCGCTGAAACGAAAGAGCCTGAGGCGAAACGGCTTGACCTCGCCGGCACAATGCTGCTGTCTCTTGCGATCTTAT
CTTGTATGATCGGTATCACGAATCTCGATGGGGCGAAGCTGTCAGAAAGCATAATGGACGTAAAGGTATACGGGTATCTT
TTGGGAGCTGCACTTCTCTTTCTGGTGCTGCTTAAGGTCGAAAAGCGGGTTGAAAACCGAGGGGGCGATCCGATTCTCGC
CTACTCGCTGCTGCAAAATAAAGTGTTTCAATGGACGCTCGTCATCGGATTTTTATCGGGAGGGCTGCTCGCTGCGGTCA
TTTTTATTCCTTCTTATGCGGAGCAATATTTGCACGTATCGGCGGAGAAAGCGGGTTATTGGATGACGCCGCTCGCGTTG
GCGTCAGGCATCGGAGCGTGGCTCGGCGGGGCGATCACGGATAAAAAAGGTCCGGTCTTTTCCGCGGTTGTATCAGGCGC
CATCGCATTTGCAGGCTTCAGCCTGTTTCCGTTATGGGTGACGGATAAATGGGAATTTGTCACAGCGAGTGTCATTGCGG
GGATCGGTTTCGGATTTCTGCTTGGGGCTCCGCTGAATGTGCTTGTATCAGAAACGGCGGGCCAAAACAAAGGAACAGCG
CTCGGCACGCTGTCACTCGTAAGGCAGGTCGGGCTGACGCTCGCTCCGGCTTTATATGCCGGCTTCATTACAGCGGGCTT
TGACCGGATCGGAGATGACATCAAAAACCGTTTAGCGAAAAGCGGCATTCCCGCTGATCAGGCGCAAATGCCCGACATCG
GCGGCGGACTTTCTTCGTTGCAGGAGCAGGTAAGCCGAATCCCCATTCCCGCCGTCAAAAAAGCGGTTTCTGATGCAATT
GAAGCGGGCATTGCCAGCGGCTACCACCATCTTTATACGGCAGCCGCCGTCATTTCATTATGCACGATTGTGTCTGCGGC
GGTTTTGGCGTATTTCCGGAAGCCGGCATCCTCTAAGACAGATGCCAGCCCATCCGCCGGGAAAGCTCAGTAA

Upstream 100 bases:

>100_bases
AAGGCCTGCCGGTATCGGCGGCCTTTTTTTGTTTGACATTTAATTCACTTGAAGTGAATAATATATTCGAAGTGAATTAA
AAACGGGAGGATTGATCAGT

Downstream 100 bases:

>100_bases
TATGGGCGATGTGATGTTCAGAGTGCCAAACATATAGCCCGAGCGCTTGTTCCAGCGTAACCGTCTCTTTAGATACAGGA
TGGAAGTATGTCCGTTTGAA

Product: YfiU

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 530; Mature: 529

Protein sequence:

>530_residues
MPEPLQTEKNQSRAITLFTIGVFMAALDNGIISAALTTINSSFDVSPSWGSWGVTLYTLGLSVSVPIVGKLSDRYGRKKL
FMIEVGLFGLGSLLVALSQSFPMFLAARLIQALGGGGIFIIGSSHVLAVLPKEKQGKALGLLGAMNGMAAVLGPNIGSFL
LDVTGSWHWLFLINLPIAAALVVCGGLFIAETKEPEAKRLDLAGTMLLSLAILSCMIGITNLDGAKLSESIMDVKVYGYL
LGAALLFLVLLKVEKRVENRGGDPILAYSLLQNKVFQWTLVIGFLSGGLLAAVIFIPSYAEQYLHVSAEKAGYWMTPLAL
ASGIGAWLGGAITDKKGPVFSAVVSGAIAFAGFSLFPLWVTDKWEFVTASVIAGIGFGFLLGAPLNVLVSETAGQNKGTA
LGTLSLVRQVGLTLAPALYAGFITAGFDRIGDDIKNRLAKSGIPADQAQMPDIGGGLSSLQEQVSRIPIPAVKKAVSDAI
EAGIASGYHHLYTAAAVISLCTIVSAAVLAYFRKPASSKTDASPSAGKAQ

Sequences:

>Translated_530_residues
MPEPLQTEKNQSRAITLFTIGVFMAALDNGIISAALTTINSSFDVSPSWGSWGVTLYTLGLSVSVPIVGKLSDRYGRKKL
FMIEVGLFGLGSLLVALSQSFPMFLAARLIQALGGGGIFIIGSSHVLAVLPKEKQGKALGLLGAMNGMAAVLGPNIGSFL
LDVTGSWHWLFLINLPIAAALVVCGGLFIAETKEPEAKRLDLAGTMLLSLAILSCMIGITNLDGAKLSESIMDVKVYGYL
LGAALLFLVLLKVEKRVENRGGDPILAYSLLQNKVFQWTLVIGFLSGGLLAAVIFIPSYAEQYLHVSAEKAGYWMTPLAL
ASGIGAWLGGAITDKKGPVFSAVVSGAIAFAGFSLFPLWVTDKWEFVTASVIAGIGFGFLLGAPLNVLVSETAGQNKGTA
LGTLSLVRQVGLTLAPALYAGFITAGFDRIGDDIKNRLAKSGIPADQAQMPDIGGGLSSLQEQVSRIPIPAVKKAVSDAI
EAGIASGYHHLYTAAAVISLCTIVSAAVLAYFRKPASSKTDASPSAGKAQ
>Mature_529_residues
PEPLQTEKNQSRAITLFTIGVFMAALDNGIISAALTTINSSFDVSPSWGSWGVTLYTLGLSVSVPIVGKLSDRYGRKKLF
MIEVGLFGLGSLLVALSQSFPMFLAARLIQALGGGGIFIIGSSHVLAVLPKEKQGKALGLLGAMNGMAAVLGPNIGSFLL
DVTGSWHWLFLINLPIAAALVVCGGLFIAETKEPEAKRLDLAGTMLLSLAILSCMIGITNLDGAKLSESIMDVKVYGYLL
GAALLFLVLLKVEKRVENRGGDPILAYSLLQNKVFQWTLVIGFLSGGLLAAVIFIPSYAEQYLHVSAEKAGYWMTPLALA
SGIGAWLGGAITDKKGPVFSAVVSGAIAFAGFSLFPLWVTDKWEFVTASVIAGIGFGFLLGAPLNVLVSETAGQNKGTAL
GTLSLVRQVGLTLAPALYAGFITAGFDRIGDDIKNRLAKSGIPADQAQMPDIGGGLSSLQEQVSRIPIPAVKKAVSDAIE
AGIASGYHHLYTAAAVISLCTIVSAAVLAYFRKPASSKTDASPSAGKAQ

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=259, Percent_Identity=32.046332046332, Blast_Score=116, Evalue=4e-27,
Organism=Escherichia coli, GI87081983, Length=427, Percent_Identity=25.7611241217799, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI1788710, Length=311, Percent_Identity=27.9742765273312, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1788392, Length=208, Percent_Identity=31.7307692307692, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1789042, Length=302, Percent_Identity=27.1523178807947, Blast_Score=86, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6323735, Length=444, Percent_Identity=23.6486486486486, Blast_Score=77, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6322958, Length=376, Percent_Identity=25.7978723404255, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6325455, Length=152, Percent_Identity=33.5526315789474, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319770, Length=450, Percent_Identity=23.1111111111111, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6321663, Length=143, Percent_Identity=30.7692307692308, Blast_Score=64, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 55382; Mature: 55250

Theoretical pI: Translated: 9.15; Mature: 9.15

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEPLQTEKNQSRAITLFTIGVFMAALDNGIISAALTTINSSFDVSPSWGSWGVTLYTLG
CCCCCCCCCCCCCEEEEHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEC
LSVSVPIVGKLSDRYGRKKLFMIEVGLFGLGSLLVALSQSFPMFLAARLIQALGGGGIFI
CEEECCEEECCHHHCCCCEEEEEEECHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEE
IGSSHVLAVLPKEKQGKALGLLGAMNGMAAVLGPNIGSFLLDVTGSWHWLFLINLPIAAA
EECCCEEEEECCCCCCCEEEHHHHHCCHHHHHCCCHHHHHHCCCCCEEEEEEEHHHHHHH
LVVCGGLFIAETKEPEAKRLDLAGTMLLSLAILSCMIGITNLDGAKLSESIMDVKVYGYL
HHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
LGAALLFLVLLKVEKRVENRGGDPILAYSLLQNKVFQWTLVIGFLSGGLLAAVIFIPSYA
HHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EQYLHVSAEKAGYWMTPLALASGIGAWLGGAITDKKGPVFSAVVSGAIAFAGFSLFPLWV
HHHHHHHHHCCCCEECHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEE
TDKWEFVTASVIAGIGFGFLLGAPLNVLVSETAGQNKGTALGTLSLVRQVGLTLAPALYA
CCCHHHHHHHHHHHHHHHHHHCCCHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
GFITAGFDRIGDDIKNRLAKSGIPADQAQMPDIGGGLSSLQEQVSRIPIPAVKKAVSDAI
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHH
EAGIASGYHHLYTAAAVISLCTIVSAAVLAYFRKPASSKTDASPSAGKAQ
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PEPLQTEKNQSRAITLFTIGVFMAALDNGIISAALTTINSSFDVSPSWGSWGVTLYTLG
CCCCCCCCCCCCEEEEHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEC
LSVSVPIVGKLSDRYGRKKLFMIEVGLFGLGSLLVALSQSFPMFLAARLIQALGGGGIFI
CEEECCEEECCHHHCCCCEEEEEEECHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEE
IGSSHVLAVLPKEKQGKALGLLGAMNGMAAVLGPNIGSFLLDVTGSWHWLFLINLPIAAA
EECCCEEEEECCCCCCCEEEHHHHHCCHHHHHCCCHHHHHHCCCCCEEEEEEEHHHHHHH
LVVCGGLFIAETKEPEAKRLDLAGTMLLSLAILSCMIGITNLDGAKLSESIMDVKVYGYL
HHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
LGAALLFLVLLKVEKRVENRGGDPILAYSLLQNKVFQWTLVIGFLSGGLLAAVIFIPSYA
HHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EQYLHVSAEKAGYWMTPLALASGIGAWLGGAITDKKGPVFSAVVSGAIAFAGFSLFPLWV
HHHHHHHHHCCCCEECHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEE
TDKWEFVTASVIAGIGFGFLLGAPLNVLVSETAGQNKGTALGTLSLVRQVGLTLAPALYA
CCCHHHHHHHHHHHHHHHHHHCCCHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
GFITAGFDRIGDDIKNRLAKSGIPADQAQMPDIGGGLSSLQEQVSRIPIPAVKKAVSDAI
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHH
EAGIASGYHHLYTAAAVISLCTIVSAAVLAYFRKPASSKTDASPSAGKAQ
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8946165; 9384377 [H]