Click here to switch to the map view.

The map label for this gene is gabR [H]

Identifier: 154684883

GI number: 154684883

Start: 411252

End: 412661

Strand: Reverse

Name: gabR [H]

Synonym: RBAM_004140

Alternate gene names: 154684883

Gene position: 412661-411252 (Counterclockwise)

Preceding gene: 154684888

Following gene: 154684881

Centisome position: 10.53

GC content: 49.43

Gene sequence:

>1410_bases
ATGCTGACGATTTCCATTGATCATTCGGAAACGGCGGATTATATCTATCAGCAGATCTATCAGCACATAAAAGCGGCCGT
TTTGAAGCGCAGCCTGATGCCTCATACAAAGGTGCCGTCTAAACGGGAGCTTGCCGAGACACTCAAGGTCAGCGTGAATT
CAGTGAATTCAGCTTATCAGCAGCTGCTTTCAGAAGGTTATTTATATGCAGTCGAAAGAAAAGGTTTTTTCGTGGAGGAA
ATTGATACGGTTTATCATGAAGAAACGCCTTCGCTCCTATTGCCTGATAATTTGAAAGAGCGGGAGACGGATAAAAGCGG
CTGGATTTCGTTTTCTCATATGAGCGTGGATACGGCGCATTTCCCCCTGAAAAGCTGGTTCCGCTGTGAACATAAAGCAG
CCGCCCGTTCATTTCATACACTCGGAGAACTCGTCCACCCGCAAGGTTTATATGAAGTCCGTGAAACAATTTCGCGGCTT
ATTTCCCTGACAAGGGGCGTTACATGCCGCCCGGAACAAATCGTGCTCGGAGCGGGCACGCAATTGCTGGTGCAGCTGCT
GACGGAACTGCTGCCAAAAGACGCACTCTACGCCATGGAGAACCCGGGATATATGAGGATGTACCGGCTGCTGCAAAACC
TCGGGAAAAAGGTTGCGACGATCAGCCTTGATGAAAAAGGCATGTCCATGGACGAAATCGAAAAACGCCGGCCCTCTGTA
TTGATCACCACGCCTTCCCATCAATTCCCCTCAGGCGTGATCATGCCGGCTTCCCGGCGGATTCAGCTTTTAAACTGGGC
CGCCGAACAGAACGGCCGCTTCATCATTGAAGATGATTATGACAGTGAATTTAAATATGACGCCGATACAATACCGGCGC
TTCAAAGCCTTGACCGGTTTCAAAATGTCATCTACATGGGGACCTTTTCAAAATCCCTTTTCCCAGGTCTGCGCATCAGC
TATATGGTTCTGCCGCCCGGGCTTTTGCGGACGTTCAAAGAGAGAACGTATGACCTGCATACGTGCAGCACGCTTGCCCA
GCTCGCGCTGAAAGAATTTATCGAATCAGGTGAATATCAGAAACATATTAAAAAGATGAAGCAGCATTATAAGAACATAC
GGGAACGCCTCATTTCGGCTTTGGAAGAAACATTCGGAGAAAGAATCAGTGTAAAGGGCGCCAATGCGGGGCTTCATTTT
GTCACTGAGTTTGACTGCGCCCGTTCAGAAAAAGAGATATTGGCACGGGCGGATGCAGAACAGCTCGATCTGTTTGCGAT
GAGCCGGTTCCGTCTGAAAGCGGGAGGCGATTCAAACAAACCGCAGCTTATCCTGGGATTCGCCCGCCTGAAGGAGAGCG
ATATTCAAGAAGGCGTCTACAGGCTTTACCGGGCGGTTTTCGGCGCATAA

Upstream 100 bases:

>100_bases
ATCCCCCTGCATTGTTTTCTTATCATTCTGACTTCTTTTTGGTATGATGAAAAGTACCAAAATCAATTTATCCATGGTAT
CAGAAAGGGGCAGGGTCTGG

Downstream 100 bases:

>100_bases
AAAAACCCCCGGAATTCGGGGACTTATTTACCAGCGGTCCGGTTCTGTCTGCAAAATCGCCTCCAGCAGGCCGGGAAACG
CCGCTTGTAAATCATCTTTG

Product: GabR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MLTISIDHSETADYIYQQIYQHIKAAVLKRSLMPHTKVPSKRELAETLKVSVNSVNSAYQQLLSEGYLYAVERKGFFVEE
IDTVYHEETPSLLLPDNLKERETDKSGWISFSHMSVDTAHFPLKSWFRCEHKAAARSFHTLGELVHPQGLYEVRETISRL
ISLTRGVTCRPEQIVLGAGTQLLVQLLTELLPKDALYAMENPGYMRMYRLLQNLGKKVATISLDEKGMSMDEIEKRRPSV
LITTPSHQFPSGVIMPASRRIQLLNWAAEQNGRFIIEDDYDSEFKYDADTIPALQSLDRFQNVIYMGTFSKSLFPGLRIS
YMVLPPGLLRTFKERTYDLHTCSTLAQLALKEFIESGEYQKHIKKMKQHYKNIRERLISALEETFGERISVKGANAGLHF
VTEFDCARSEKEILARADAEQLDLFAMSRFRLKAGGDSNKPQLILGFARLKESDIQEGVYRLYRAVFGA

Sequences:

>Translated_469_residues
MLTISIDHSETADYIYQQIYQHIKAAVLKRSLMPHTKVPSKRELAETLKVSVNSVNSAYQQLLSEGYLYAVERKGFFVEE
IDTVYHEETPSLLLPDNLKERETDKSGWISFSHMSVDTAHFPLKSWFRCEHKAAARSFHTLGELVHPQGLYEVRETISRL
ISLTRGVTCRPEQIVLGAGTQLLVQLLTELLPKDALYAMENPGYMRMYRLLQNLGKKVATISLDEKGMSMDEIEKRRPSV
LITTPSHQFPSGVIMPASRRIQLLNWAAEQNGRFIIEDDYDSEFKYDADTIPALQSLDRFQNVIYMGTFSKSLFPGLRIS
YMVLPPGLLRTFKERTYDLHTCSTLAQLALKEFIESGEYQKHIKKMKQHYKNIRERLISALEETFGERISVKGANAGLHF
VTEFDCARSEKEILARADAEQLDLFAMSRFRLKAGGDSNKPQLILGFARLKESDIQEGVYRLYRAVFGA
>Mature_469_residues
MLTISIDHSETADYIYQQIYQHIKAAVLKRSLMPHTKVPSKRELAETLKVSVNSVNSAYQQLLSEGYLYAVERKGFFVEE
IDTVYHEETPSLLLPDNLKERETDKSGWISFSHMSVDTAHFPLKSWFRCEHKAAARSFHTLGELVHPQGLYEVRETISRL
ISLTRGVTCRPEQIVLGAGTQLLVQLLTELLPKDALYAMENPGYMRMYRLLQNLGKKVATISLDEKGMSMDEIEKRRPSV
LITTPSHQFPSGVIMPASRRIQLLNWAAEQNGRFIIEDDYDSEFKYDADTIPALQSLDRFQNVIYMGTFSKSLFPGLRIS
YMVLPPGLLRTFKERTYDLHTCSTLAQLALKEFIESGEYQKHIKKMKQHYKNIRERLISALEETFGERISVKGANAGLHF
VTEFDCARSEKEILARADAEQLDLFAMSRFRLKAGGDSNKPQLILGFARLKESDIQEGVYRLYRAVFGA

Specific function: Activates the transcription of the gabTD operon. Is also a repressor of its own expression, both in the presence and absence of GABA. Binds specifically to the DNA region overlapping the -35 region of the gabT promoter and the -10 and +1 regions of the ga

COG id: COG1167

COG function: function code KE; Transcriptional regulators containing a DNA-binding HTH domain and an aminotransferase domain (MocR family) and their eukaryotic orthologs

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790797, Length=440, Percent_Identity=25.2272727272727, Blast_Score=102, Evalue=8e-23,
Organism=Escherichia coli, GI1787710, Length=413, Percent_Identity=23.4866828087167, Blast_Score=98, Evalue=1e-21,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004839
- InterPro:   IPR000524
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00155 Aminotran_1_2; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 53652; Mature: 53652

Theoretical pI: Translated: 8.02; Mature: 8.02

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTISIDHSETADYIYQQIYQHIKAAVLKRSLMPHTKVPSKRELAETLKVSVNSVNSAYQ
CEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
QLLSEGYLYAVERKGFFVEEIDTVYHEETPSLLLPDNLKERETDKSGWISFSHMSVDTAH
HHHHCCCEEEEECCCCHHHHHHHHHHCCCCCEECCCCCHHHCCCCCCCEEEEECCCCCHH
FPLKSWFRCEHKAAARSFHTLGELVHPQGLYEVRETISRLISLTRGVTCRPEQIVLGAGT
CCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCH
QLLVQLLTELLPKDALYAMENPGYMRMYRLLQNLGKKVATISLDEKGMSMDEIEKRRPSV
HHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHCCCE
LITTPSHQFPSGVIMPASRRIQLLNWAAEQNGRFIIEDDYDSEFKYDADTIPALQSLDRF
EEECCCCCCCCCEECCCCCCEEHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHH
QNVIYMGTFSKSLFPGLRISYMVLPPGLLRTFKERTYDLHTCSTLAQLALKEFIESGEYQ
HHEEEEECCHHHHCCCCEEEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHH
KHIKKMKQHYKNIRERLISALEETFGERISVKGANAGLHFVTEFDCARSEKEILARADAE
HHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEEEEEHHHHCCHHHHHHHCCHH
QLDLFAMSRFRLKAGGDSNKPQLILGFARLKESDIQEGVYRLYRAVFGA
HHHHHHHHHHHEECCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLTISIDHSETADYIYQQIYQHIKAAVLKRSLMPHTKVPSKRELAETLKVSVNSVNSAYQ
CEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
QLLSEGYLYAVERKGFFVEEIDTVYHEETPSLLLPDNLKERETDKSGWISFSHMSVDTAH
HHHHCCCEEEEECCCCHHHHHHHHHHCCCCCEECCCCCHHHCCCCCCCEEEEECCCCCHH
FPLKSWFRCEHKAAARSFHTLGELVHPQGLYEVRETISRLISLTRGVTCRPEQIVLGAGT
CCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCH
QLLVQLLTELLPKDALYAMENPGYMRMYRLLQNLGKKVATISLDEKGMSMDEIEKRRPSV
HHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHCCCE
LITTPSHQFPSGVIMPASRRIQLLNWAAEQNGRFIIEDDYDSEFKYDADTIPALQSLDRF
EEECCCCCCCCCEECCCCCCEEHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHH
QNVIYMGTFSKSLFPGLRISYMVLPPGLLRTFKERTYDLHTCSTLAQLALKEFIESGEYQ
HHEEEEECCHHHHCCCCEEEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHH
KHIKKMKQHYKNIRERLISALEETFGERISVKGANAGLHFVTEFDCARSEKEILARADAE
HHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEEEEEHHHHCCHHHHHHHCCHH
QLDLFAMSRFRLKAGGDSNKPQLILGFARLKESDIQEGVYRLYRAVFGA
HHHHHHHHHHHEECCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377 [H]