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The map label for this gene is lmrB [H]

Identifier: 154684767

GI number: 154684767

Start: 268331

End: 269764

Strand: Reverse

Name: lmrB [H]

Synonym: RBAM_002980

Alternate gene names: 154684767

Gene position: 269764-268331 (Counterclockwise)

Preceding gene: 154684768

Following gene: 154684765

Centisome position: 6.88

GC content: 51.12

Gene sequence:

>1434_bases
TTGGAAAACGCTCAGCCTTCCAAACAATATAAAGTCATGCCGATCATGATTTCTTTACTGACGGCAGGGTTTATCGGCAT
GTTCAGTGAAACGGCGCTGAATATCGCCCTGACTGACCTGATGAAGGAGTTACATATCACTCCTGCGACCGTCCAGTGGC
TGACGACGGGCTATCTGCTCGTTCTCGGTATTCTTGTCCCGGTATCGGGTCTGCTTTTGCAATGGTTTACGACGAGACAG
CTGTTTATCGTTTCACTTGCTTTTTCCATTATCGGCACGCTGATTGCAGCGCTTGCGCCGAGCTTTCCGTTTCTTTTGGC
GGCGAGAATCGTGCAGGCTTTAGGAACCGGGCTGCTGCTGCCGCTGATGTTTAATACAATCCTCGTCATCTTCCCGCCGC
ATAAGCGCGGAGCGGCGATGGGGACGATCGGGCTTGTCATCATGTTCGCTCCTGCGATCGGTCCGACATTTTCAGGGCTT
GTGTTAGAGCATTTGAACTGGCATTGGATTTTCTGGATTTCCCTGCCGTTTCTTGTGTTGGCTCTTTTATTCGGCATCGC
CTACATGCAGAACGTGTCTGACATTACAAAACCGAAAATTGACGTGCTTTCCATTATTCTGTCTACGATCGGGTTCGGCG
GCATCGTATTCGGATTCAGCAATGCGGGCGAAGGATCAGACGGCTGGTCAAGCCCGACGGTTATCGGTTCGCTCACTGTC
GGTGCGATCGCGCTCATCTTATTTTCCATCAGGCAGCTGACAATGAAGCAGCCGATGATGAATCTGCGCGCTTTCCGCTA
TCCGATGTTTGTTCTAGGTGTCGTGATTGTATTTATCTGTATGATGGTTATCTTATCAACAATGCTCCTATTGCCTATGT
ATTTACAAAGCGGACTGATGCTGACGGCGTTTACGTCCGGGCTCATCCTGCTGCCGGGCGGTATTTTAAACGGCTTTATG
TCTCCGGTGACGGGGCGTCTGTTTGATAAATACGGGCCGAAGTGGCTTGTCATTCCCGGGTTTGTCATCACGGCAACTGC
GCTTTCGTTTTTCTCAAACATTAACGGCGCTTCAACGGCGCTTCTGATTGTCGCGCTTCACACATGCCTGATGATCGGCA
TTTCCATGATCATGATGCCGGCGCAAACGAACGGCTTAAACCAGCTGCCGCCGGAATTTTATCCGGACGGAACGGCCATT
ATGAATACGCTCCAGCAGATGGCCGGCGCAATCGGCACTGCGGTGGCGGTCAGCATCATGGCAGCCGGGCAAAAGAATTA
TATGAGCACCGTGAAAAACCCGAATGATCCTTCCGCATACTCTCACGCGCTGATTGCGGGCGTCCAGCACGCCTTTATCT
TCGCGATGATTGTCGCTGTTATCGGCTTGATCAGCGCCTTCTTTATGAAACGGGTCAAAGTGAATCACTCATAA

Upstream 100 bases:

>100_bases
AGACAAAATGCCGATCCTCCATATTTCTAAACAAATCCCGAACCTGCTGAAGACATAAAATACGAGAGACATGTACGTAT
AGAAAGAGAGGAGAATGATT

Downstream 100 bases:

>100_bases
GAAAACAGCAGGCTCCCCGTGAGTCTGCTGTTTTTTTATATACCGCGCGCTTCAGCCAGTTCATCAATCGCATACATCAG
ATCATCGGCCGTGTTATAGA

Product: LmrB

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MENAQPSKQYKVMPIMISLLTAGFIGMFSETALNIALTDLMKELHITPATVQWLTTGYLLVLGILVPVSGLLLQWFTTRQ
LFIVSLAFSIIGTLIAALAPSFPFLLAARIVQALGTGLLLPLMFNTILVIFPPHKRGAAMGTIGLVIMFAPAIGPTFSGL
VLEHLNWHWIFWISLPFLVLALLFGIAYMQNVSDITKPKIDVLSIILSTIGFGGIVFGFSNAGEGSDGWSSPTVIGSLTV
GAIALILFSIRQLTMKQPMMNLRAFRYPMFVLGVVIVFICMMVILSTMLLLPMYLQSGLMLTAFTSGLILLPGGILNGFM
SPVTGRLFDKYGPKWLVIPGFVITATALSFFSNINGASTALLIVALHTCLMIGISMIMMPAQTNGLNQLPPEFYPDGTAI
MNTLQQMAGAIGTAVAVSIMAAGQKNYMSTVKNPNDPSAYSHALIAGVQHAFIFAMIVAVIGLISAFFMKRVKVNHS

Sequences:

>Translated_477_residues
MENAQPSKQYKVMPIMISLLTAGFIGMFSETALNIALTDLMKELHITPATVQWLTTGYLLVLGILVPVSGLLLQWFTTRQ
LFIVSLAFSIIGTLIAALAPSFPFLLAARIVQALGTGLLLPLMFNTILVIFPPHKRGAAMGTIGLVIMFAPAIGPTFSGL
VLEHLNWHWIFWISLPFLVLALLFGIAYMQNVSDITKPKIDVLSIILSTIGFGGIVFGFSNAGEGSDGWSSPTVIGSLTV
GAIALILFSIRQLTMKQPMMNLRAFRYPMFVLGVVIVFICMMVILSTMLLLPMYLQSGLMLTAFTSGLILLPGGILNGFM
SPVTGRLFDKYGPKWLVIPGFVITATALSFFSNINGASTALLIVALHTCLMIGISMIMMPAQTNGLNQLPPEFYPDGTAI
MNTLQQMAGAIGTAVAVSIMAAGQKNYMSTVKNPNDPSAYSHALIAGVQHAFIFAMIVAVIGLISAFFMKRVKVNHS
>Mature_477_residues
MENAQPSKQYKVMPIMISLLTAGFIGMFSETALNIALTDLMKELHITPATVQWLTTGYLLVLGILVPVSGLLLQWFTTRQ
LFIVSLAFSIIGTLIAALAPSFPFLLAARIVQALGTGLLLPLMFNTILVIFPPHKRGAAMGTIGLVIMFAPAIGPTFSGL
VLEHLNWHWIFWISLPFLVLALLFGIAYMQNVSDITKPKIDVLSIILSTIGFGGIVFGFSNAGEGSDGWSSPTVIGSLTV
GAIALILFSIRQLTMKQPMMNLRAFRYPMFVLGVVIVFICMMVILSTMLLLPMYLQSGLMLTAFTSGLILLPGGILNGFM
SPVTGRLFDKYGPKWLVIPGFVITATALSFFSNINGASTALLIVALHTCLMIGISMIMMPAQTNGLNQLPPEFYPDGTAI
MNTLQQMAGAIGTAVAVSIMAAGQKNYMSTVKNPNDPSAYSHALIAGVQHAFIFAMIVAVIGLISAFFMKRVKVNHS

Specific function: Proton-dependent transporter. May mediate the efflux of lincomycin [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=455, Percent_Identity=24.8351648351648, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1788710, Length=428, Percent_Identity=24.7663551401869, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI87081983, Length=344, Percent_Identity=26.7441860465116, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI1790195, Length=402, Percent_Identity=23.8805970149254, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1788392, Length=400, Percent_Identity=26.25, Blast_Score=84, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6325455, Length=232, Percent_Identity=28.0172413793103, Blast_Score=84, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 51419; Mature: 51419

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
6.1 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
6.1 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENAQPSKQYKVMPIMISLLTAGFIGMFSETALNIALTDLMKELHITPATVQWLTTGYLL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
VLGILVPVSGLLLQWFTTRQLFIVSLAFSIIGTLIAALAPSFPFLLAARIVQALGTGLLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
PLMFNTILVIFPPHKRGAAMGTIGLVIMFAPAIGPTFSGLVLEHLNWHWIFWISLPFLVL
HHHHCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHH
ALLFGIAYMQNVSDITKPKIDVLSIILSTIGFGGIVFGFSNAGEGSDGWSSPTVIGSLTV
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHEEECCCCCCCCCCCCCCCHHHHHHH
GAIALILFSIRQLTMKQPMMNLRAFRYPMFVLGVVIVFICMMVILSTMLLLPMYLQSGLM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LTAFTSGLILLPGGILNGFMSPVTGRLFDKYGPKWLVIPGFVITATALSFFSNINGASTA
HHHHHCCHHEECCHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHCCCCHHHH
LLIVALHTCLMIGISMIMMPAQTNGLNQLPPEFYPDGTAIMNTLQQMAGAIGTAVAVSIM
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AAGQKNYMSTVKNPNDPSAYSHALIAGVQHAFIFAMIVAVIGLISAFFMKRVKVNHS
HCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MENAQPSKQYKVMPIMISLLTAGFIGMFSETALNIALTDLMKELHITPATVQWLTTGYLL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
VLGILVPVSGLLLQWFTTRQLFIVSLAFSIIGTLIAALAPSFPFLLAARIVQALGTGLLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
PLMFNTILVIFPPHKRGAAMGTIGLVIMFAPAIGPTFSGLVLEHLNWHWIFWISLPFLVL
HHHHCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHH
ALLFGIAYMQNVSDITKPKIDVLSIILSTIGFGGIVFGFSNAGEGSDGWSSPTVIGSLTV
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHEEECCCCCCCCCCCCCCCHHHHHHH
GAIALILFSIRQLTMKQPMMNLRAFRYPMFVLGVVIVFICMMVILSTMLLLPMYLQSGLM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LTAFTSGLILLPGGILNGFMSPVTGRLFDKYGPKWLVIPGFVITATALSFFSNINGASTA
HHHHHCCHHEECCHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHCCCCHHHH
LLIVALHTCLMIGISMIMMPAQTNGLNQLPPEFYPDGTAIMNTLQQMAGAIGTAVAVSIM
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AAGQKNYMSTVKNPNDPSAYSHALIAGVQHAFIFAMIVAVIGLISAFFMKRVKVNHS
HCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9274031; 9384377 [H]