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The map label for this gene is gatA [H]

Identifier: 154248623

GI number: 154248623

Start: 5218349

End: 5220139

Strand: Direct

Name: gatA [H]

Synonym: Xaut_4705

Alternate gene names: 154248623

Gene position: 5218349-5220139 (Clockwise)

Preceding gene: 154248622

Following gene: 154248624

Centisome position: 98.29

GC content: 71.69

Gene sequence:

>1791_bases
ATGCAGACCCTCGCCAGCCTCCTTTCCGCCCACAGGTCGGGCACCCGCTCGGTGACCGACACGGTACGCGCCGTCTATGC
CCGCATCGCGGTGCAGGACGATCCGGCGATGTTCATCACCCTGCGTGCGGAAGCCGATGTGCTGGCGGAAGCCGCCGCCC
TGGACGCGAAGGGCGACACCGCCCTGCCCCTCTATGGCATCCCGGTGGCGGTGAAGGACAATATCGACGTGGCCGGCCTG
CCCACCACCTGCGCCTGCCCCGCCTTCGCCTATACGCCCGAGCACGACGCCACCGCTGTGGCGCGGCTGAAGGCGGCGGG
CGCGCTCATCATCGGCAAGACCAATCTGGACCAGTTCGCCACCGGCCTCGTGGGCGTGCGCTCGCCCTATGGCATCCCGC
GCAACGCCTTCAATCCGGTGCTGGTGCCCGGTGGCTCGTCCTCCGGCTCGGCGTCGGCGGTTTCGGCGGGCATCGTGCCG
GTGTCGCTCGGCACCGACACCGCCGGCTCCGGCCGGGTGCCGGCCATGCTGCAGAACATCGTGGGGCTGAAGCCCTCCCT
CGGCCTCGTGCCCAACACCGGCCTCGTGCCCGCCTGCAAGACGCTGGACTGCATCTCCATCTTCGCCCTTACGGTGGACG
ACGCCATGGCGGTGCTGGATGTCATCGCCGGCTTCGATCCGCAGGATGCCTGGTCCCGCGACCTGCCGGTCGGCCCGCTT
GCTGCCGCGCCACCGTCCTTCGTGCTGGGCGTGCTGCCGGAAGCGCAGCGCGAATTCTTCGGCGATGCCCACGCCGCCGC
CGCCTATGAAGCGGCCCTCGCCCGCTTCAAGGCCATCGGCGCGACGCTGAAGGAGATCGATTTTTCCCCCTTCGCCGAGA
CCGCCCGCCTGCTTTATGAAGGCCCCTGGGTGGCGGAGCGCTGGATCGTGGCGGAGGAGCTGCTCACCACCACGCCCGAG
GCGCTGCATCCCGTCACCCGCGCCATCACCGAGCCGGGCGGCAAGGCCAGCGCCGCCGACGCCTTCCGCGCCACCTACCG
GCTGAAGGAACTCAGGGCGAAGGTGGCCCCGGTGCTCGCCGCCATCGACGGCCTGCTGCTGCCCACCGCGCCCTCCGCCT
ACACGGTGGAGGAGGTGCTGGCCGATCCGGTGCGCCTCAATTCGCGCAATGGCACCTACACCAATTTCGTGAACCTCATG
GACCTGTGCGGCATCGCCGTGCCGGCCGCCATTGCGCCCACCGGCGTGCCCTATGGCGTCACCCTGCTGGCGCCGGCCGG
CAAGGACGCGCAGATGGCGGCGCTGGGCCGGGCCTTCGTGGGCGCCGGCGACCTGCCGCTGGGGGCGAGCCACGCCCCCG
CCCCGCCCCTCGCGCCCCTGCCGGTGGCGGCAGAGCCGGGCCGTCTCGACATCCTGGTTTTCGGCGCCCACCTCTCCGGC
ATGGCGCTGAACGGGGATCTGAAAGCCATGGGCGGGCGCTTCGTGCGCACCGCGACCACTGCCCCCACCTATCGCATGAA
GCGCCTGCCCGGTGCCGTGGCGCGCCCCGGCGTGGTGCGGGTGAAGGCGGACGGCGCGGTTCTGGAAGGCGAGATCTGGT
CGCTGCCTTTCGAGGGCGTCGGGAAGCTGCTCGCCACCATTCCCGCCCCCCTCGGGCTTGGGCAGGTGGAACTGGCGGAC
GGCACGCGCGTGGCCGGCTTCCTTGCCGAGGCGGCCGCCCTCGAGGGCGCTGAAGACATCACCGCGCACGGCGGCTTCCG
CGCCTTCATGGCGGCGGCGCAGACCGCCTGA

Upstream 100 bases:

>100_bases
ACTATATAATATTTAATATTAAATAGAAAATTGCATACACATGGCGCGTTTCCTGCTAGGCCTTGCCCGCCGCGCGGTGC
GGTCGAACCCGGGTTAGAGC

Downstream 100 bases:

>100_bases
GGACCTGCCCCAAGGCCAAGGCACGAACGCGTGAGCCTGCTGGACAGGGGGACTGATACACTGTATCAAGAGCGCCGTTG
AACCACTTTCCGGCAGGTTC

Product: allophanate hydrolase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 596; Mature: 596

Protein sequence:

>596_residues
MQTLASLLSAHRSGTRSVTDTVRAVYARIAVQDDPAMFITLRAEADVLAEAAALDAKGDTALPLYGIPVAVKDNIDVAGL
PTTCACPAFAYTPEHDATAVARLKAAGALIIGKTNLDQFATGLVGVRSPYGIPRNAFNPVLVPGGSSSGSASAVSAGIVP
VSLGTDTAGSGRVPAMLQNIVGLKPSLGLVPNTGLVPACKTLDCISIFALTVDDAMAVLDVIAGFDPQDAWSRDLPVGPL
AAAPPSFVLGVLPEAQREFFGDAHAAAAYEAALARFKAIGATLKEIDFSPFAETARLLYEGPWVAERWIVAEELLTTTPE
ALHPVTRAITEPGGKASAADAFRATYRLKELRAKVAPVLAAIDGLLLPTAPSAYTVEEVLADPVRLNSRNGTYTNFVNLM
DLCGIAVPAAIAPTGVPYGVTLLAPAGKDAQMAALGRAFVGAGDLPLGASHAPAPPLAPLPVAAEPGRLDILVFGAHLSG
MALNGDLKAMGGRFVRTATTAPTYRMKRLPGAVARPGVVRVKADGAVLEGEIWSLPFEGVGKLLATIPAPLGLGQVELAD
GTRVAGFLAEAAALEGAEDITAHGGFRAFMAAAQTA

Sequences:

>Translated_596_residues
MQTLASLLSAHRSGTRSVTDTVRAVYARIAVQDDPAMFITLRAEADVLAEAAALDAKGDTALPLYGIPVAVKDNIDVAGL
PTTCACPAFAYTPEHDATAVARLKAAGALIIGKTNLDQFATGLVGVRSPYGIPRNAFNPVLVPGGSSSGSASAVSAGIVP
VSLGTDTAGSGRVPAMLQNIVGLKPSLGLVPNTGLVPACKTLDCISIFALTVDDAMAVLDVIAGFDPQDAWSRDLPVGPL
AAAPPSFVLGVLPEAQREFFGDAHAAAAYEAALARFKAIGATLKEIDFSPFAETARLLYEGPWVAERWIVAEELLTTTPE
ALHPVTRAITEPGGKASAADAFRATYRLKELRAKVAPVLAAIDGLLLPTAPSAYTVEEVLADPVRLNSRNGTYTNFVNLM
DLCGIAVPAAIAPTGVPYGVTLLAPAGKDAQMAALGRAFVGAGDLPLGASHAPAPPLAPLPVAAEPGRLDILVFGAHLSG
MALNGDLKAMGGRFVRTATTAPTYRMKRLPGAVARPGVVRVKADGAVLEGEIWSLPFEGVGKLLATIPAPLGLGQVELAD
GTRVAGFLAEAAALEGAEDITAHGGFRAFMAAAQTA
>Mature_596_residues
MQTLASLLSAHRSGTRSVTDTVRAVYARIAVQDDPAMFITLRAEADVLAEAAALDAKGDTALPLYGIPVAVKDNIDVAGL
PTTCACPAFAYTPEHDATAVARLKAAGALIIGKTNLDQFATGLVGVRSPYGIPRNAFNPVLVPGGSSSGSASAVSAGIVP
VSLGTDTAGSGRVPAMLQNIVGLKPSLGLVPNTGLVPACKTLDCISIFALTVDDAMAVLDVIAGFDPQDAWSRDLPVGPL
AAAPPSFVLGVLPEAQREFFGDAHAAAAYEAALARFKAIGATLKEIDFSPFAETARLLYEGPWVAERWIVAEELLTTTPE
ALHPVTRAITEPGGKASAADAFRATYRLKELRAKVAPVLAAIDGLLLPTAPSAYTVEEVLADPVRLNSRNGTYTNFVNLM
DLCGIAVPAAIAPTGVPYGVTLLAPAGKDAQMAALGRAFVGAGDLPLGASHAPAPPLAPLPVAAEPGRLDILVFGAHLSG
MALNGDLKAMGGRFVRTATTAPTYRMKRLPGAVARPGVVRVKADGAVLEGEIWSLPFEGVGKLLATIPAPLGLGQVELAD
GTRVAGFLAEAAALEGAEDITAHGGFRAFMAAAQTA

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=506, Percent_Identity=28.2608695652174, Blast_Score=125, Evalue=9e-29,
Organism=Homo sapiens, GI195972892, Length=185, Percent_Identity=32.972972972973, Blast_Score=88, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17543272, Length=413, Percent_Identity=29.0556900726392, Blast_Score=133, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17537465, Length=198, Percent_Identity=29.2929292929293, Blast_Score=82, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17556264, Length=211, Percent_Identity=27.0142180094787, Blast_Score=71, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17538252, Length=180, Percent_Identity=26.1111111111111, Blast_Score=65, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6319685, Length=570, Percent_Identity=41.5789473684211, Blast_Score=390, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6323950, Length=215, Percent_Identity=34.8837209302326, Blast_Score=93, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24648113, Length=174, Percent_Identity=45.9770114942529, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24652985, Length=189, Percent_Identity=29.6296296296296, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI19922090, Length=189, Percent_Identity=29.6296296296296, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24652981, Length=189, Percent_Identity=29.6296296296296, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24652983, Length=189, Percent_Identity=29.6296296296296, Blast_Score=70, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 61087; Mature: 61087

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTLASLLSAHRSGTRSVTDTVRAVYARIAVQDDPAMFITLRAEADVLAEAAALDAKGDT
CHHHHHHHHHHCCCCCHHHHHHHHHHHHHEECCCCEEEEEEECCHHHHHHHHHCCCCCCC
ALPLYGIPVAVKDNIDVAGLPTTCACPAFAYTPEHDATAVARLKAAGALIIGKTNLDQFA
CCEEECCCEEEECCCCCCCCCCHHCCCCCEECCCCCHHHHHHHHHCCEEEEECCCHHHHH
TGLVGVRSPYGIPRNAFNPVLVPGGSSSGSASAVSAGIVPVSLGTDTAGSGRVPAMLQNI
HHHHCCCCCCCCCCCCCCCEEEECCCCCCCCHHHCCCEEEEECCCCCCCCCCHHHHHHHH
VGLKPSLGLVPNTGLVPACKTLDCISIFALTVDDAMAVLDVIAGFDPQDAWSRDLPVGPL
HCCCCCCCCCCCCCCCCCHHHHHHHHHEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
AAAPPSFVLGVLPEAQREFFGDAHAAAAYEAALARFKAIGATLKEIDFSPFAETARLLYE
CCCCHHHHEECCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHC
GPWVAERWIVAEELLTTTPEALHPVTRAITEPGGKASAADAFRATYRLKELRAKVAPVLA
CCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AIDGLLLPTAPSAYTVEEVLADPVRLNSRNGTYTNFVNLMDLCGIAVPAAIAPTGVPYGV
HHCCCCCCCCCCCEEHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCE
TLLAPAGKDAQMAALGRAFVGAGDLPLGASHAPAPPLAPLPVAAEPGRLDILVFGAHLSG
EEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCC
MALNGDLKAMGGRFVRTATTAPTYRMKRLPGAVARPGVVRVKADGAVLEGEIWSLPFEGV
EEECCCHHHCCCEEEEEECCCCCHHHHHCCCCCCCCCEEEEECCCEEEECCEEECCHHHH
GKLLATIPAPLGLGQVELADGTRVAGFLAEAAALEGAEDITAHGGFRAFMAAAQTA
HHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHCCC
>Mature Secondary Structure
MQTLASLLSAHRSGTRSVTDTVRAVYARIAVQDDPAMFITLRAEADVLAEAAALDAKGDT
CHHHHHHHHHHCCCCCHHHHHHHHHHHHHEECCCCEEEEEEECCHHHHHHHHHCCCCCCC
ALPLYGIPVAVKDNIDVAGLPTTCACPAFAYTPEHDATAVARLKAAGALIIGKTNLDQFA
CCEEECCCEEEECCCCCCCCCCHHCCCCCEECCCCCHHHHHHHHHCCEEEEECCCHHHHH
TGLVGVRSPYGIPRNAFNPVLVPGGSSSGSASAVSAGIVPVSLGTDTAGSGRVPAMLQNI
HHHHCCCCCCCCCCCCCCCEEEECCCCCCCCHHHCCCEEEEECCCCCCCCCCHHHHHHHH
VGLKPSLGLVPNTGLVPACKTLDCISIFALTVDDAMAVLDVIAGFDPQDAWSRDLPVGPL
HCCCCCCCCCCCCCCCCCHHHHHHHHHEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
AAAPPSFVLGVLPEAQREFFGDAHAAAAYEAALARFKAIGATLKEIDFSPFAETARLLYE
CCCCHHHHEECCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHC
GPWVAERWIVAEELLTTTPEALHPVTRAITEPGGKASAADAFRATYRLKELRAKVAPVLA
CCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AIDGLLLPTAPSAYTVEEVLADPVRLNSRNGTYTNFVNLMDLCGIAVPAAIAPTGVPYGV
HHCCCCCCCCCCCEEHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCE
TLLAPAGKDAQMAALGRAFVGAGDLPLGASHAPAPPLAPLPVAAEPGRLDILVFGAHLSG
EEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCC
MALNGDLKAMGGRFVRTATTAPTYRMKRLPGAVARPGVVRVKADGAVLEGEIWSLPFEGV
EEECCCHHHCCCEEEEEECCCCCHHHHHCCCCCCCCCEEEEECCCEEEECCEEECCHHHH
GKLLATIPAPLGLGQVELADGTRVAGFLAEAAALEGAEDITAHGGFRAFMAAAQTA
HHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12566566 [H]