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The map label for this gene is purU

Identifier: 154248622

GI number: 154248622

Start: 5216969

End: 5217838

Strand: Direct

Name: purU

Synonym: Xaut_4704

Alternate gene names: 154248622

Gene position: 5216969-5217838 (Clockwise)

Preceding gene: 154248621

Following gene: 154248623

Centisome position: 98.27

GC content: 64.37

Gene sequence:

>870_bases
ATGACCACGCCGAGCGATCATTTCATCCTCACCTTCTCCTGCCCGAACAAGCCGGGCATCGTGGCGGGGGTGTCCACCTT
CCTGTTCGAGAAGGGCTGCAACATCCTCGAGGCTCAGCAGTTCGACGACACTGAAACCAACCGCTTCTTCATGCGCGTGG
TGTTCAACGTGGTGGCGAGCGAGGCGAGCCTGTCCGAGATCCGCGAGGGCTTCACCGCCGTGGCCGAGGGCTTCGCGCTG
AACTGGACGCTGCGGCCCAAGGCTGAGAAGCGCCGGGTGCTGCTGCTGGCCTCCAAGTTCGACCATTGCCTCGCCGACCT
GCTCTACCGCTGGCGCATCGGCGAGATCCCCATGGATATCACCGGCATCATCTCCAACCATCCGCGCGAGACCTATGCCC
ATCTCGATTTCGATGGCATCCCCTTCCATCACCTGCCGGTGACCAAGGCCACCAAGCTGGAGCAGGAAACGAAGATCTGG
GAGATCTTCCAGTCGTCCGGCTCGGAGCTGGCGGTGCTCGCGCGCTACATGCAGGTGCTGTCCGACGGGCTCACTGCGAA
GCTCTCGGGCAAGTGCATCAACATCCACCACTCGTTCCTGCCCGGCTTCAAGGGCGCCAAGCCCTATCACCAGGCCCACG
CGCGCGGCGTGAAGCTGATGGGCGCCACCTCGCACTACGTGACCTCGGATCTCGACGAGGGGCCGATCATCGAACAGGAC
GTGGAGCGCATCTCCCACCAGGATTCCCCCGAAGACCTGGTGCGCAAGGGCCGCGACATCGAGCGCCGCGTGCTCGCCCG
CGCCATCTCCTGGCACCTGCAGGACCGGGTGCTGCTGAACGGTTCGCGCACCGTGGTGTTCCGCGACTGA

Upstream 100 bases:

>100_bases
GACCGCAGCGCGGCGCGCGCGACATCCCTTCGCCCCTCACCTTTCCTCACGCGACCGCCCACGCCCCGGCGCGCGGCGCG
CCAGCCAAAGACGCATCCCA

Downstream 100 bases:

>100_bases
CCGCCTCCAAAAGGCCCAAAGGCGGCCGGTAGGGAAACCACCGGCCGCCCGGGGGCGTTGAACAGGGGAGATCCACCACC
AGGGAGAACCCCATGCCCCG

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MTTPSDHFILTFSCPNKPGIVAGVSTFLFEKGCNILEAQQFDDTETNRFFMRVVFNVVASEASLSEIREGFTAVAEGFAL
NWTLRPKAEKRRVLLLASKFDHCLADLLYRWRIGEIPMDITGIISNHPRETYAHLDFDGIPFHHLPVTKATKLEQETKIW
EIFQSSGSELAVLARYMQVLSDGLTAKLSGKCINIHHSFLPGFKGAKPYHQAHARGVKLMGATSHYVTSDLDEGPIIEQD
VERISHQDSPEDLVRKGRDIERRVLARAISWHLQDRVLLNGSRTVVFRD

Sequences:

>Translated_289_residues
MTTPSDHFILTFSCPNKPGIVAGVSTFLFEKGCNILEAQQFDDTETNRFFMRVVFNVVASEASLSEIREGFTAVAEGFAL
NWTLRPKAEKRRVLLLASKFDHCLADLLYRWRIGEIPMDITGIISNHPRETYAHLDFDGIPFHHLPVTKATKLEQETKIW
EIFQSSGSELAVLARYMQVLSDGLTAKLSGKCINIHHSFLPGFKGAKPYHQAHARGVKLMGATSHYVTSDLDEGPIIEQD
VERISHQDSPEDLVRKGRDIERRVLARAISWHLQDRVLLNGSRTVVFRD
>Mature_288_residues
TTPSDHFILTFSCPNKPGIVAGVSTFLFEKGCNILEAQQFDDTETNRFFMRVVFNVVASEASLSEIREGFTAVAEGFALN
WTLRPKAEKRRVLLLASKFDHCLADLLYRWRIGEIPMDITGIISNHPRETYAHLDFDGIPFHHLPVTKATKLEQETKIWE
IFQSSGSELAVLARYMQVLSDGLTAKLSGKCINIHHSFLPGFKGAKPYHQAHARGVKLMGATSHYVTSDLDEGPIIEQDV
ERISHQDSPEDLVRKGRDIERRVLARAISWHLQDRVLLNGSRTVVFRD

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=188, Percent_Identity=30.3191489361702, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI209869995, Length=188, Percent_Identity=30.3191489361702, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI209869993, Length=188, Percent_Identity=30.3191489361702, Blast_Score=72, Evalue=5e-13,
Organism=Escherichia coli, GI1787483, Length=279, Percent_Identity=39.0681003584229, Blast_Score=207, Evalue=8e-55,
Organism=Escherichia coli, GI1788846, Length=112, Percent_Identity=31.25, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17567511, Length=171, Percent_Identity=30.4093567251462, Blast_Score=79, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 32674; Mature: 32543

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPSDHFILTFSCPNKPGIVAGVSTFLFEKGCNILEAQQFDDTETNRFFMRVVFNVVAS
CCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHC
EASLSEIREGFTAVAEGFALNWTLRPKAEKRRVLLLASKFDHCLADLLYRWRIGEIPMDI
CHHHHHHHHHHHHHHCCEEEEEEECCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCH
TGIISNHPRETYAHLDFDGIPFHHLPVTKATKLEQETKIWEIFQSSGSELAVLARYMQVL
HHHHCCCCCHHEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
SDGLTAKLSGKCINIHHSFLPGFKGAKPYHQAHARGVKLMGATSHYVTSDLDEGPIIEQD
HCCCEEEECCCEEEEEHHHCCCCCCCCCHHHHHHCCEEEEECCCHHHHCCCCCCCCCHHH
VERISHQDSPEDLVRKGRDIERRVLARAISWHLQDRVLLNGSRTVVFRD
HHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHCCEEEECCCEEEEEEC
>Mature Secondary Structure 
TTPSDHFILTFSCPNKPGIVAGVSTFLFEKGCNILEAQQFDDTETNRFFMRVVFNVVAS
CCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHC
EASLSEIREGFTAVAEGFALNWTLRPKAEKRRVLLLASKFDHCLADLLYRWRIGEIPMDI
CHHHHHHHHHHHHHHCCEEEEEEECCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCH
TGIISNHPRETYAHLDFDGIPFHHLPVTKATKLEQETKIWEIFQSSGSELAVLARYMQVL
HHHHCCCCCHHEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
SDGLTAKLSGKCINIHHSFLPGFKGAKPYHQAHARGVKLMGATSHYVTSDLDEGPIIEQD
HCCCEEEECCCEEEEEHHHCCCCCCCCCHHHHHHCCEEEEECCCHHHHCCCCCCCCCHHH
VERISHQDSPEDLVRKGRDIERRVLARAISWHLQDRVLLNGSRTVVFRD
HHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHCCEEEECCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7543100 [H]