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The map label for this gene is glnT [H]

Identifier: 154248616

GI number: 154248616

Start: 5209234

End: 5210595

Strand: Direct

Name: glnT [H]

Synonym: Xaut_4698

Alternate gene names: 154248616

Gene position: 5209234-5210595 (Clockwise)

Preceding gene: 154248615

Following gene: 154248618

Centisome position: 98.12

GC content: 64.32

Gene sequence:

>1362_bases
ATGACCAGCAGAACCGAATTCGCACGGGAGGCGGAAGGGCGCGCCTCCGTCTCCGCCAAGGTGAGCCTCGACCTTGCCAA
GATCGCCAAGGAACGCGGCATCAAGTATTTCCTCATCTCCTACGTGGACCTGTTCGGCGGCCTGCGGGCCAAGCTCGTGC
CCGCCTCGGCCATCGGCCCCATGCAGAAGGAAGGCGCCGGATTTGCCGGCTTCGCCACCTGGCTCGACATGTCTCCGGCC
GACCCGGACCTGTTCGCCGTGCCTGACCCCACCAGCCTCATCCAGCTGCCGTGGAAAGAGGAAATCGGCTGGCTCGCCGC
CGACCTCTACATGGGCGGCGAGGAAGTGGCGCAGGGCCCGCGCACCGTTCTGAAGCGCCAGCTCGCCGCCGCCGCAGCGC
TGGGCTACGAGGTGAAGACCGGCGTGGAGTGCGAGTATTTCCTGATCACGCCAGACGGCAAGGGCATCTCCGATACGTCG
GATACTGCGGAAAAGCCCTGCTACGACCAGTCCGCCCTCATGCGTCGCTACGATGTCATCAAGGACATCTGCGATGCCAT
GATCAAGCTCGGCTGGGGCGCCTACCAGAACGACCACGAGGACGCCAACGGCCAGTTCGAGATGAACTGGAACTATGACA
ATGCGTTGCTCACCGCCGACCGCCACGTCTTCTTCAAGTACATGGTGAAGTCGATCGCGGAACAGCATGGCCTGCGCGCC
ACCTTCATGCCCAAGCCCTTCATCAACCTCACCGGCTCGGGCTGCCATGCCCATGTCTCGCTGTGGTCGAAGGGCAAGAA
TGCCTTCTCCGATCCCAAGGGCGAGCTGGGCGTCTCGCAGCTCGGCTACCATTTCATCGGCGGGCTGATCCACAATGCGG
ACGCGCTGTGCGCCATCACCAATCCCACGGTGAATTCCTACAAGCGCATCAACGCTCCGCGCACCATCTCCGGGGCCACC
TGGGCGCCGAACACGGTGACCTACACCGGCAACAACCGCACCCACATGATCCGCATTCCGGACGCCGGCCGGTTCGAATT
CCGCCTCGCCGACGGCGCGGCGAACCCCTACCTGCTCCAGGCGGGCCTGACGGCGGCGGGCCTCGACGGCATCGAGAACC
AGCGCGATCCGGGCAAGCGCCTCGACATCAACATGTACACGGACGGGCACAAGGTGAAGGGCGCCAAGCGCCTGCCCCTG
AACATGCTCGACGCCCTGCGCGCGCTGGAAAAGTCTCGCGTGCTGGGCGAGCGGCTGGGCCAGCCCTTCGTCACCGGCTA
TCTCAAGCTGAAGAACGAGGAATGGAACGCATATTCCCGCCACCTCACCCAGTGGGAGCGGGACAACACGCTGGATATCT
GA

Upstream 100 bases:

>100_bases
TACTGAGCCGCGTTTTCCTGACTTCGGCCGCGCCGCTCGACCGGCGCGGCCGCCATAAAAATCCTGAACATCCAAGACCT
TCCTGCCAGGGATCGAACCC

Downstream 100 bases:

>100_bases
GCTGCGGAATAGCTCCCGCTCGCGACGGGCCCCGGAGGCAGCTCCGGGGCCTTTGTCGTTGGCACGGCGGGTCCCGCAGA
ACCGTGCTTATTCGGTGGTC

Product: glutamine synthetase catalytic subunit

Products: NA

Alternate protein names: Glutamate--ammonia ligase; Glutamine synthetase III; GSIII [H]

Number of amino acids: Translated: 453; Mature: 452

Protein sequence:

>453_residues
MTSRTEFAREAEGRASVSAKVSLDLAKIAKERGIKYFLISYVDLFGGLRAKLVPASAIGPMQKEGAGFAGFATWLDMSPA
DPDLFAVPDPTSLIQLPWKEEIGWLAADLYMGGEEVAQGPRTVLKRQLAAAAALGYEVKTGVECEYFLITPDGKGISDTS
DTAEKPCYDQSALMRRYDVIKDICDAMIKLGWGAYQNDHEDANGQFEMNWNYDNALLTADRHVFFKYMVKSIAEQHGLRA
TFMPKPFINLTGSGCHAHVSLWSKGKNAFSDPKGELGVSQLGYHFIGGLIHNADALCAITNPTVNSYKRINAPRTISGAT
WAPNTVTYTGNNRTHMIRIPDAGRFEFRLADGAANPYLLQAGLTAAGLDGIENQRDPGKRLDINMYTDGHKVKGAKRLPL
NMLDALRALEKSRVLGERLGQPFVTGYLKLKNEEWNAYSRHLTQWERDNTLDI

Sequences:

>Translated_453_residues
MTSRTEFAREAEGRASVSAKVSLDLAKIAKERGIKYFLISYVDLFGGLRAKLVPASAIGPMQKEGAGFAGFATWLDMSPA
DPDLFAVPDPTSLIQLPWKEEIGWLAADLYMGGEEVAQGPRTVLKRQLAAAAALGYEVKTGVECEYFLITPDGKGISDTS
DTAEKPCYDQSALMRRYDVIKDICDAMIKLGWGAYQNDHEDANGQFEMNWNYDNALLTADRHVFFKYMVKSIAEQHGLRA
TFMPKPFINLTGSGCHAHVSLWSKGKNAFSDPKGELGVSQLGYHFIGGLIHNADALCAITNPTVNSYKRINAPRTISGAT
WAPNTVTYTGNNRTHMIRIPDAGRFEFRLADGAANPYLLQAGLTAAGLDGIENQRDPGKRLDINMYTDGHKVKGAKRLPL
NMLDALRALEKSRVLGERLGQPFVTGYLKLKNEEWNAYSRHLTQWERDNTLDI
>Mature_452_residues
TSRTEFAREAEGRASVSAKVSLDLAKIAKERGIKYFLISYVDLFGGLRAKLVPASAIGPMQKEGAGFAGFATWLDMSPAD
PDLFAVPDPTSLIQLPWKEEIGWLAADLYMGGEEVAQGPRTVLKRQLAAAAALGYEVKTGVECEYFLITPDGKGISDTSD
TAEKPCYDQSALMRRYDVIKDICDAMIKLGWGAYQNDHEDANGQFEMNWNYDNALLTADRHVFFKYMVKSIAEQHGLRAT
FMPKPFINLTGSGCHAHVSLWSKGKNAFSDPKGELGVSQLGYHFIGGLIHNADALCAITNPTVNSYKRINAPRTISGATW
APNTVTYTGNNRTHMIRIPDAGRFEFRLADGAANPYLLQAGLTAAGLDGIENQRDPGKRLDINMYTDGHKVKGAKRLPLN
MLDALRALEKSRVLGERLGQPFVTGYLKLKNEEWNAYSRHLTQWERDNTLDI

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family. Type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=428, Percent_Identity=23.8317757009346, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI1790301, Length=270, Percent_Identity=37.037037037037, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI87081870, Length=449, Percent_Identity=29.3986636971047, Blast_Score=133, Evalue=2e-32,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR017536 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 50049; Mature: 49918

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSRTEFAREAEGRASVSAKVSLDLAKIAKERGIKYFLISYVDLFGGLRAKLVPASAIGP
CCCHHHHHHHCCCCCCEEEEEEHHHHHHHHHCCCEEEHHHHHHHHCCCCEEECCHHHCCC
MQKEGAGFAGFATWLDMSPADPDLFAVPDPTSLIQLPWKEEIGWLAADLYMGGEEVAQGP
CHHCCCCCCHHHHHCCCCCCCCCEEECCCCCCEEECCCHHHHCCEEHHHHCCCHHHHCCH
RTVLKRQLAAAAALGYEVKTGVECEYFLITPDGKGISDTSDTAEKPCYDQSALMRRYDVI
HHHHHHHHHHHHHHCCEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
KDICDAMIKLGWGAYQNDHEDANGQFEMNWNYDNALLTADRHVFFKYMVKSIAEQHGLRA
HHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCEEEECHHHHHHHHHHHHHHHHHCCEE
TFMPKPFINLTGSGCHAHVSLWSKGKNAFSDPKGELGVSQLGYHFIGGLIHNADALCAIT
EECCCCCEEECCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEC
NPTVNSYKRINAPRTISGATWAPNTVTYTGNNRTHMIRIPDAGRFEFRLADGAANPYLLQ
CCCCHHHHHCCCCCCCCCCCCCCCCEEECCCCEEEEEECCCCCCEEEEEECCCCCCEEEE
AGLTAAGLDGIENQRDPGKRLDINMYTDGHKVKGAKRLPLNMLDALRALEKSRVLGERLG
CCCCHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
QPFVTGYLKLKNEEWNAYSRHLTQWERDNTLDI
CCCEEEEEEEECCCCCHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TSRTEFAREAEGRASVSAKVSLDLAKIAKERGIKYFLISYVDLFGGLRAKLVPASAIGP
CCHHHHHHHCCCCCCEEEEEEHHHHHHHHHCCCEEEHHHHHHHHCCCCEEECCHHHCCC
MQKEGAGFAGFATWLDMSPADPDLFAVPDPTSLIQLPWKEEIGWLAADLYMGGEEVAQGP
CHHCCCCCCHHHHHCCCCCCCCCEEECCCCCCEEECCCHHHHCCEEHHHHCCCHHHHCCH
RTVLKRQLAAAAALGYEVKTGVECEYFLITPDGKGISDTSDTAEKPCYDQSALMRRYDVI
HHHHHHHHHHHHHHCCEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
KDICDAMIKLGWGAYQNDHEDANGQFEMNWNYDNALLTADRHVFFKYMVKSIAEQHGLRA
HHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCEEEECHHHHHHHHHHHHHHHHHCCEE
TFMPKPFINLTGSGCHAHVSLWSKGKNAFSDPKGELGVSQLGYHFIGGLIHNADALCAIT
EECCCCCEEECCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEC
NPTVNSYKRINAPRTISGATWAPNTVTYTGNNRTHMIRIPDAGRFEFRLADGAANPYLLQ
CCCCHHHHHCCCCCCCCCCCCCCCCEEECCCCEEEEEECCCCCCEEEEEECCCCCCEEEE
AGLTAAGLDGIENQRDPGKRLDINMYTDGHKVKGAKRLPLNMLDALRALEKSRVLGERLG
CCCCHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
QPFVTGYLKLKNEEWNAYSRHLTQWERDNTLDI
CCCEEEEEEEECCCCCHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1356885 [H]