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The map label for this gene is sseA [H]

Identifier: 154248597

GI number: 154248597

Start: 5182575

End: 5183429

Strand: Direct

Name: sseA [H]

Synonym: Xaut_4678

Alternate gene names: 154248597

Gene position: 5182575-5183429 (Clockwise)

Preceding gene: 154248596

Following gene: 154248600

Centisome position: 97.62

GC content: 69.94

Gene sequence:

>855_bases
ATGAGCGAGACAGTCGACACGCCAGCGAGCCCCTTCGTCTCCACCGAATGGCTCGCGGCCAATCTGGGCGCTCCCGACCT
TGTGGTGGTGGACGCCTCCTGGCACATGCCCAATTCCGGCCGCAGCGGCGCCAAGGAATATCTGGAGCGGCACATTCCCG
GCGCGGTGCATTTCGACATCGACACCATTGCCGACCACTCCACCCCCCTGCCCCACATGCTGCCCGACGCGCACGCCTTC
GCCGTTGCCGTGGGCGCGCTGGGCATCGGCGACGGGCTGCGCATCGTGGTCTACGATTCGGTGGGCCTGTTCTCGGCGCC
CCGGGTGTGGTGGACCTTCCGGGCCTTCGGCGCGCGTGACGTGAAGATCCTCGACGGCGGCCTGCCCAAATGGCTCGCGG
AGGAGCGCCCGGTGGAAGACGGGCCGGTGCGCCGTCTGCCCGCCACCTTCAATCCGCGCTTCGACCATTCCCTCGTCGCC
GATATCGAGCGCGTGGACCAGGCCCTGAAGAGCGGCAGCGCACAGGTGCTCGACGCCCGCCAGGACACCCGCTTCCGCGG
CGAGGCGCCCGAGCCGCGCGAGGGCCTGCCTTCCGGCCACATGCCCGGCAGCTTCAACCTGCCCCACGGCCGCCTCGTGA
AGGACGGGCGGCTGGTGTCGCCGGAGGAGATCCGCACGGTGGTCGAAGCCTCCGGGCTCGACCTGTCCCGTCCAGTCATC
ACCTCGTGCGGATCCGGCGTATCGGCGGCCATCCTGTGGGTGGCGCTTGAGCTGATCGGCAAGACGCCGCTCGCCCTCTA
TGACGGCTCGTGGACCGAGTGGGGCGCCAGCGGCAAGGAGATCGCGGTCGGCTGA

Upstream 100 bases:

>100_bases
CGGCGCGCCTTGCGCCGGCCGCTTGACGCGCGTGCCCATCCGCTCAATGAGGGGCCGCGCGCCCTTGCCGGTGCCTGAAC
GGAGTCGAAAGAGACAGCCC

Downstream 100 bases:

>100_bases
CGGGCCGGTCTTCGCACCTCAAGTCTATCACGCTCCTGCCGATCAGATCGCGATGCGATCTGATCGGACTGCGTCAGGCC
ACGCTGCTGGCGGCGGCCCG

Product: rhodanese domain-containing protein

Products: NA

Alternate protein names: MST; Rhodanese-like protein [H]

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MSETVDTPASPFVSTEWLAANLGAPDLVVVDASWHMPNSGRSGAKEYLERHIPGAVHFDIDTIADHSTPLPHMLPDAHAF
AVAVGALGIGDGLRIVVYDSVGLFSAPRVWWTFRAFGARDVKILDGGLPKWLAEERPVEDGPVRRLPATFNPRFDHSLVA
DIERVDQALKSGSAQVLDARQDTRFRGEAPEPREGLPSGHMPGSFNLPHGRLVKDGRLVSPEEIRTVVEASGLDLSRPVI
TSCGSGVSAAILWVALELIGKTPLALYDGSWTEWGASGKEIAVG

Sequences:

>Translated_284_residues
MSETVDTPASPFVSTEWLAANLGAPDLVVVDASWHMPNSGRSGAKEYLERHIPGAVHFDIDTIADHSTPLPHMLPDAHAF
AVAVGALGIGDGLRIVVYDSVGLFSAPRVWWTFRAFGARDVKILDGGLPKWLAEERPVEDGPVRRLPATFNPRFDHSLVA
DIERVDQALKSGSAQVLDARQDTRFRGEAPEPREGLPSGHMPGSFNLPHGRLVKDGRLVSPEEIRTVVEASGLDLSRPVI
TSCGSGVSAAILWVALELIGKTPLALYDGSWTEWGASGKEIAVG
>Mature_283_residues
SETVDTPASPFVSTEWLAANLGAPDLVVVDASWHMPNSGRSGAKEYLERHIPGAVHFDIDTIADHSTPLPHMLPDAHAFA
VAVGALGIGDGLRIVVYDSVGLFSAPRVWWTFRAFGARDVKILDGGLPKWLAEERPVEDGPVRRLPATFNPRFDHSLVAD
IERVDQALKSGSAQVLDARQDTRFRGEAPEPREGLPSGHMPGSFNLPHGRLVKDGRLVSPEEIRTVVEASGLDLSRPVIT
SCGSGVSAAILWVALELIGKTPLALYDGSWTEWGASGKEIAVG

Specific function: Transfers a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. Its participation in detoxification of cyanide may be small. May be involved in the enhancement of serine sensitivity [H]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI194473668, Length=285, Percent_Identity=44.2105263157895, Blast_Score=241, Evalue=4e-64,
Organism=Homo sapiens, GI61835204, Length=270, Percent_Identity=44.8148148148148, Blast_Score=241, Evalue=6e-64,
Organism=Homo sapiens, GI194473681, Length=270, Percent_Identity=44.8148148148148, Blast_Score=241, Evalue=6e-64,
Organism=Homo sapiens, GI17402865, Length=271, Percent_Identity=45.3874538745387, Blast_Score=224, Evalue=8e-59,
Organism=Escherichia coli, GI87082121, Length=266, Percent_Identity=42.8571428571429, Blast_Score=229, Evalue=1e-61,
Organism=Escherichia coli, GI87081967, Length=282, Percent_Identity=26.241134751773, Blast_Score=71, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17561888, Length=301, Percent_Identity=26.578073089701, Blast_Score=113, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71997283, Length=300, Percent_Identity=25.6666666666667, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17543836, Length=290, Percent_Identity=25.5172413793103, Blast_Score=84, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17543838, Length=290, Percent_Identity=25.5172413793103, Blast_Score=84, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17559150, Length=245, Percent_Identity=26.530612244898, Blast_Score=81, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI115534702, Length=196, Percent_Identity=25.5102040816327, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6324825, Length=281, Percent_Identity=36.6548042704626, Blast_Score=163, Evalue=2e-41,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.2 [H]

Molecular weight: Translated: 30520; Mature: 30389

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS00380 RHODANESE_1 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETVDTPASPFVSTEWLAANLGAPDLVVVDASWHMPNSGRSGAKEYLERHIPGAVHFDI
CCCCCCCCCCCCCCCHHHEECCCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCEEEEEH
DTIADHSTPLPHMLPDAHAFAVAVGALGIGDGLRIVVYDSVGLFSAPRVWWTFRAFGARD
HHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCEEEEECCCCCC
VKILDGGLPKWLAEERPVEDGPVRRLPATFNPRFDHSLVADIERVDQALKSGSAQVLDAR
EEEECCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECC
QDTRFRGEAPEPREGLPSGHMPGSFNLPHGRLVKDGRLVSPEEIRTVVEASGLDLSRPVI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHCCCCCCCCHH
TSCGSGVSAAILWVALELIGKTPLALYDGSWTEWGASGKEIAVG
HHCCCCHHHHHHHHHHHHHCCCCEEEECCCCHHCCCCCCEEECC
>Mature Secondary Structure 
SETVDTPASPFVSTEWLAANLGAPDLVVVDASWHMPNSGRSGAKEYLERHIPGAVHFDI
CCCCCCCCCCCCCCHHHEECCCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCEEEEEH
DTIADHSTPLPHMLPDAHAFAVAVGALGIGDGLRIVVYDSVGLFSAPRVWWTFRAFGARD
HHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCEEEEECCCCCC
VKILDGGLPKWLAEERPVEDGPVRRLPATFNPRFDHSLVADIERVDQALKSGSAQVLDAR
EEEECCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECC
QDTRFRGEAPEPREGLPSGHMPGSFNLPHGRLVKDGRLVSPEEIRTVVEASGLDLSRPVI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHCCCCCCCCHH
TSCGSGVSAAILWVALELIGKTPLALYDGSWTEWGASGKEIAVG
HHCCCCHHHHHHHHHHHHHCCCCEEEECCCCHHCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]