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The map label for this gene is rimO

Identifier: 154248395

GI number: 154248395

Start: 4968808

End: 4970157

Strand: Direct

Name: rimO

Synonym: Xaut_4475

Alternate gene names: 154248395

Gene position: 4968808-4970157 (Clockwise)

Preceding gene: 154248394

Following gene: 154248399

Centisome position: 93.59

GC content: 68.74

Gene sequence:

>1350_bases
ATGGCCGATACCGTCCTGAACTCCGCGCCATCCAAGGCTGCGCCCCGCATCTCCTTCGTCTCGCTGGGCTGCCCCAAGGC
ACTGGTGGATTCAGAACGCATCGTCACCCGGCTGCGCGCGGAAGGCTATGAGCTGACCAAGACCCACCAGGGCGCGGACC
TCGTGGTGGTGAACACCTGCGGCTTCCTCGACAGCGCCAAGGCGGAAAGCCTCAATGCCATCGGCGAGGCGCTGGCCGAG
AATGGCAAGGTGGTGGTCACCGGCTGCATGGGCGCCGAGCCCGAGCAGATCCGCGAGGTCCACCCGAGCGTCCTCGCCAT
CACCGGCCCGCAGCAATACGAGAGCGTGCTCGCCGCCGTGCACGAGGCGGTGCCGCCGGCCCACGATCCCTTCCTCGACC
TCGTGCCCGCCGAGGGCGTCAAGCTCACGCCCCGCCACTATGCCTATCTCAAGATTTCCGAGGGCTGCTCCAACCGCTGC
ACCTTCTGCATCATCCCCAAGCTGCGCGGCGACCTGGTCTCGCGCCCGGCGGGGGACGTGCTGCGGGAGGCCGAGCGGCT
GGTGAAGGCGGGCGTGAAGGAACTGCTCGTCATCTCGCAGGACACCTCCGCCTATGGCGTTGACCTGCGCTACGCCACCA
GCCCGTGGGGCGACAAGGAAGTCGCCGCCCGCTTCCTGGATCTGGCCGGCGCGCTGGGGGACCTCGGCGCATGGGTGCGC
CTGCACTACGTCTACCCCTACCCGCACGTGGATGCGGTGATGGAGTTGATGGCCGCCGGCAAGGTGCTGCCCTATCTCGA
CATCCCGTTCCAGCATGCCAGCCCCACCGTGCTGAAGCGCATGAAGCGCCCGGCCTCGCAGGAAAAGACGCTGGCCCGCA
TCCAGGCGTGGCGCGCGGCGGTGCCGGACCTCACGCTGCGCTCCACCTTCATCGTCGGCTTCCCCGGCGAGACCGAGGCG
GAGTTCGAGGAACTGCTCGGCTTCCTCGAAGAGGCGGAGATCGACCGCGCCGGCTGCTTCAAGTTCGAGCCGGTGCGCGG
GGCCGATGCCAATGCGCTGGAAAACCCCGTGCCCGACGCGGTGAAGGCAGAACGCTACGACCGCTTCATGCGCACCCAGC
AGAAGGTTTCCGCCCGGCGCCTGAAGCGCAAGGTGGGCACCCGCCAGAGCGTGATCATCGATACCGTGACGCCCGACGGC
GGCATCGGCCGCACCAAGGGCGACGCGCCGGAGATCGACGGCACGGTGAAGGTGTTCGCCCGCCGTCCGCTGCGCGTGGG
CGAGATCGCCACCGTGAAGATCGAGGCGGCCGGGCCTTACGACCTCTCCGGCACCGCCGTGGGGTTCTGA

Upstream 100 bases:

>100_bases
TTCGGCCACAAAGTCGCGGGATTGACGGAGCGCGGGCGCTCGGTTACGCGCCAGCCCTTCGCACAGCTCCCATCTTGCCC
AAGAGAAACCCGCCTGCCCC

Downstream 100 bases:

>100_bases
GGGGCGCGCCCCTCAGGGCGTGGCCGGCTCCAGCTCCGCCGCCACGCGCTCCAGAATATCCGCCGCGGCGGATATCTCCG
CCTCCGTCACCGTGAGCGGC

Product: ribosomal protein S12 methylthiotransferase

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MADTVLNSAPSKAAPRISFVSLGCPKALVDSERIVTRLRAEGYELTKTHQGADLVVVNTCGFLDSAKAESLNAIGEALAE
NGKVVVTGCMGAEPEQIREVHPSVLAITGPQQYESVLAAVHEAVPPAHDPFLDLVPAEGVKLTPRHYAYLKISEGCSNRC
TFCIIPKLRGDLVSRPAGDVLREAERLVKAGVKELLVISQDTSAYGVDLRYATSPWGDKEVAARFLDLAGALGDLGAWVR
LHYVYPYPHVDAVMELMAAGKVLPYLDIPFQHASPTVLKRMKRPASQEKTLARIQAWRAAVPDLTLRSTFIVGFPGETEA
EFEELLGFLEEAEIDRAGCFKFEPVRGADANALENPVPDAVKAERYDRFMRTQQKVSARRLKRKVGTRQSVIIDTVTPDG
GIGRTKGDAPEIDGTVKVFARRPLRVGEIATVKIEAAGPYDLSGTAVGF

Sequences:

>Translated_449_residues
MADTVLNSAPSKAAPRISFVSLGCPKALVDSERIVTRLRAEGYELTKTHQGADLVVVNTCGFLDSAKAESLNAIGEALAE
NGKVVVTGCMGAEPEQIREVHPSVLAITGPQQYESVLAAVHEAVPPAHDPFLDLVPAEGVKLTPRHYAYLKISEGCSNRC
TFCIIPKLRGDLVSRPAGDVLREAERLVKAGVKELLVISQDTSAYGVDLRYATSPWGDKEVAARFLDLAGALGDLGAWVR
LHYVYPYPHVDAVMELMAAGKVLPYLDIPFQHASPTVLKRMKRPASQEKTLARIQAWRAAVPDLTLRSTFIVGFPGETEA
EFEELLGFLEEAEIDRAGCFKFEPVRGADANALENPVPDAVKAERYDRFMRTQQKVSARRLKRKVGTRQSVIIDTVTPDG
GIGRTKGDAPEIDGTVKVFARRPLRVGEIATVKIEAAGPYDLSGTAVGF
>Mature_448_residues
ADTVLNSAPSKAAPRISFVSLGCPKALVDSERIVTRLRAEGYELTKTHQGADLVVVNTCGFLDSAKAESLNAIGEALAEN
GKVVVTGCMGAEPEQIREVHPSVLAITGPQQYESVLAAVHEAVPPAHDPFLDLVPAEGVKLTPRHYAYLKISEGCSNRCT
FCIIPKLRGDLVSRPAGDVLREAERLVKAGVKELLVISQDTSAYGVDLRYATSPWGDKEVAARFLDLAGALGDLGAWVRL
HYVYPYPHVDAVMELMAAGKVLPYLDIPFQHASPTVLKRMKRPASQEKTLARIQAWRAAVPDLTLRSTFIVGFPGETEAE
FEELLGFLEEAEIDRAGCFKFEPVRGADANALENPVPDAVKAERYDRFMRTQQKVSARRLKRKVGTRQSVIIDTVTPDGG
IGRTKGDAPEIDGTVKVFARRPLRVGEIATVKIEAAGPYDLSGTAVGF

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI93277076, Length=463, Percent_Identity=27.8617710583153, Blast_Score=152, Evalue=5e-37,
Organism=Homo sapiens, GI28872784, Length=474, Percent_Identity=24.4725738396624, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI28872782, Length=474, Percent_Identity=24.4725738396624, Blast_Score=133, Evalue=3e-31,
Organism=Escherichia coli, GI1787057, Length=434, Percent_Identity=64.2857142857143, Blast_Score=578, Evalue=1e-166,
Organism=Escherichia coli, GI1786882, Length=452, Percent_Identity=28.0973451327434, Blast_Score=155, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI17553146, Length=480, Percent_Identity=25, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71996771, Length=264, Percent_Identity=29.1666666666667, Blast_Score=98, Evalue=7e-21,
Organism=Drosophila melanogaster, GI21356207, Length=482, Percent_Identity=26.3485477178423, Blast_Score=143, Evalue=3e-34,
Organism=Drosophila melanogaster, GI19922432, Length=399, Percent_Identity=26.0651629072682, Blast_Score=110, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMO_XANP2 (A7INV0)

Other databases:

- EMBL:   CP000781
- RefSeq:   YP_001419353.1
- ProteinModelPortal:   A7INV0
- SMR:   A7INV0
- STRING:   A7INV0
- GeneID:   5421988
- GenomeReviews:   CP000781_GR
- KEGG:   xau:Xaut_4475
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   KADAPEI
- ProtClustDB:   PRK14862
- BioCyc:   XAUT78245:XAUT_4475-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01865
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01125
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 48699; Mature: 48568

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADTVLNSAPSKAAPRISFVSLGCPKALVDSERIVTRLRAEGYELTKTHQGADLVVVNTC
CCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEEECC
GFLDSAKAESLNAIGEALAENGKVVVTGCMGAEPEQIREVHPSVLAITGPQQYESVLAAV
CCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHCCCCEEEEECHHHHHHHHHHH
HEAVPPAHDPFLDLVPAEGVKLTPRHYAYLKISEGCSNRCTFCIIPKLRGDLVSRPAGDV
HHHCCCCCCCCCCCCCCCCEEECCCEEEEEEECCCCCCCCEEEEECCCCCHHHCCCHHHH
LREAERLVKAGVKELLVISQDTSAYGVDLRYATSPWGDKEVAARFLDLAGALGDLGAWVR
HHHHHHHHHHCHHHEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCEEE
LHYVYPYPHVDAVMELMAAGKVLPYLDIPFQHASPTVLKRMKRPASQEKTLARIQAWRAA
EEEECCCCCHHHHHHHHHCCCCCCEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHH
VPDLTLRSTFIVGFPGETEAEFEELLGFLEEAEIDRAGCFKFEPVRGADANALENPVPDA
CCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCHHCCCCCCH
VKAERYDRFMRTQQKVSARRLKRKVGTRQSVIIDTVTPDGGIGRTKGDAPEIDGTVKVFA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCEEEEE
RRPLRVGEIATVKIEAAGPYDLSGTAVGF
CCCCCCCCEEEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure 
ADTVLNSAPSKAAPRISFVSLGCPKALVDSERIVTRLRAEGYELTKTHQGADLVVVNTC
CCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEEECC
GFLDSAKAESLNAIGEALAENGKVVVTGCMGAEPEQIREVHPSVLAITGPQQYESVLAAV
CCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHCCCCEEEEECHHHHHHHHHHH
HEAVPPAHDPFLDLVPAEGVKLTPRHYAYLKISEGCSNRCTFCIIPKLRGDLVSRPAGDV
HHHCCCCCCCCCCCCCCCCEEECCCEEEEEEECCCCCCCCEEEEECCCCCHHHCCCHHHH
LREAERLVKAGVKELLVISQDTSAYGVDLRYATSPWGDKEVAARFLDLAGALGDLGAWVR
HHHHHHHHHHCHHHEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCEEE
LHYVYPYPHVDAVMELMAAGKVLPYLDIPFQHASPTVLKRMKRPASQEKTLARIQAWRAA
EEEECCCCCHHHHHHHHHCCCCCCEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHH
VPDLTLRSTFIVGFPGETEAEFEELLGFLEEAEIDRAGCFKFEPVRGADANALENPVPDA
CCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCHHCCCCCCH
VKAERYDRFMRTQQKVSARRLKRKVGTRQSVIIDTVTPDGGIGRTKGDAPEIDGTVKVFA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCEEEEE
RRPLRVGEIATVKIEAAGPYDLSGTAVGF
CCCCCCCCEEEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA