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The map label for this gene is tyrS

Identifier: 154248394

GI number: 154248394

Start: 4967149

End: 4968399

Strand: Direct

Name: tyrS

Synonym: Xaut_4474

Alternate gene names: 154248394

Gene position: 4967149-4968399 (Clockwise)

Preceding gene: 154248391

Following gene: 154248395

Centisome position: 93.56

GC content: 66.67

Gene sequence:

>1251_bases
ATGACCACTCAGACCTTCCGCTCCGACTTCCTGCGCACGCTCCACGAGCGCGGCTACATCCACCAGTGCTCGGACCTCGA
GCGGCTCGACGCCAAGGCCGCGGAAGGGCCCATCACCGCCTATATCGGCTTCGACGCCACCGCCTCCAGCCTCCATGCCG
GGCACCTCCTGTCCATCATGATGCTGCGCACACTCCAGCGCACCGGGCACAACCCCATCGCGCTCATGGGCGGCGGCACC
ACCAAGATCGGCGACCCGTCCGGCAAGGACGAGGCCCGCAAGATGCTGACCGACCAGCAGATCGAGGACAACATCGCCTC
TATCCGCAAGGTGTTCGCGCGCTTCCTCGATTTCGGGGCGGGCGCGAAGATGGAGAACAACGCGTCCTGGCTCGACGAGC
TGAAATACATCCCGCTGCTGCGCGAGGTGGGGCCGCACTTCACCATCAACCGCATGCTCACCTTCGATTCGGTGAAGCTG
CGGCTGGAACGCGAGCAGCCGCTCACCTTCCTCGAATTCAACTACATGATCCTGCAGGCCTACGACTTCGTGGAGCTGAA
CGCCCGCCACGGCTGCATCCTGCAGATGGGCGGCTCGGACCAGTGGGGCAATATCGTCAACGGCATGGAGCTGGGCCGGC
GCATGCGCGGGGCCGACCTGTTCGCGCTGACGACGCCGCTGCTCACCACCTCCTCCGGCGCCAAGATGGGCAAGACCGCC
GGCGGCGCGGTGTGGCTCGATGCCGACCTGCTGTCGCCTTACGAATACTGGCAGTACTGGCGCAACGCCGGCGACGCCGA
CGTGGAGCGCTTCCTGAAGCTCTTCACCGAGCTGCCGCTGGACGAGATCGCCCGCCTCGCCGCGCTGGAAGGCCAGGAGA
TCAACCACGCCAAGGAAGTGCTGGCCACCGAGGCCACTGCGCTGCTGCATGGCCGCGAGGCCGCCGAGAAGGCGAAGGAG
ACCTCCCGCGCCACCTTCGCCGACGGCGCGCTGGCGGTGGACCTGCCCACGGTGGAAGTGCCGCGCGCGACGCTGGAGGC
TGGCCTTCCCGTCGCCAAAGCCTTCGTGGATGCCGGCCTCGTCGCCTCCACCAGCGAGGCGCGTCGCCAGATCAAGGGTG
GCGGCCTGAAGGTGAACGATGTGACTGTCACCGACGAGAAGGCTGTGATCGATCTCGGTGCGCTGACGCCGGAAGGCGTG
GTGAAGCTCTCGCTGGGCAAGAAGAAGCACGTGCTGCTGAAGCCGGTGTGA

Upstream 100 bases:

>100_bases
AGCGCGCCCTGTGCAATCTTGCGCGGGACCTGATACACCACCGCTCCTTTCCGGCGCGTGAGCGCCCGCCTCACGTCCGA
TCCAAAGACCGAACCCAGCG

Downstream 100 bases:

>100_bases
GGACACGCACCCCGGACGCATGCCCGGACGCAATCTCGTCCCCTCTCCCGCCCGAACTCGGCTGGTGCCGCGTTCGGTGC
CCAAGAGCCAAAGCCGGATG

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS

Number of amino acids: Translated: 416; Mature: 415

Protein sequence:

>416_residues
MTTQTFRSDFLRTLHERGYIHQCSDLERLDAKAAEGPITAYIGFDATASSLHAGHLLSIMMLRTLQRTGHNPIALMGGGT
TKIGDPSGKDEARKMLTDQQIEDNIASIRKVFARFLDFGAGAKMENNASWLDELKYIPLLREVGPHFTINRMLTFDSVKL
RLEREQPLTFLEFNYMILQAYDFVELNARHGCILQMGGSDQWGNIVNGMELGRRMRGADLFALTTPLLTTSSGAKMGKTA
GGAVWLDADLLSPYEYWQYWRNAGDADVERFLKLFTELPLDEIARLAALEGQEINHAKEVLATEATALLHGREAAEKAKE
TSRATFADGALAVDLPTVEVPRATLEAGLPVAKAFVDAGLVASTSEARRQIKGGGLKVNDVTVTDEKAVIDLGALTPEGV
VKLSLGKKKHVLLKPV

Sequences:

>Translated_416_residues
MTTQTFRSDFLRTLHERGYIHQCSDLERLDAKAAEGPITAYIGFDATASSLHAGHLLSIMMLRTLQRTGHNPIALMGGGT
TKIGDPSGKDEARKMLTDQQIEDNIASIRKVFARFLDFGAGAKMENNASWLDELKYIPLLREVGPHFTINRMLTFDSVKL
RLEREQPLTFLEFNYMILQAYDFVELNARHGCILQMGGSDQWGNIVNGMELGRRMRGADLFALTTPLLTTSSGAKMGKTA
GGAVWLDADLLSPYEYWQYWRNAGDADVERFLKLFTELPLDEIARLAALEGQEINHAKEVLATEATALLHGREAAEKAKE
TSRATFADGALAVDLPTVEVPRATLEAGLPVAKAFVDAGLVASTSEARRQIKGGGLKVNDVTVTDEKAVIDLGALTPEGV
VKLSLGKKKHVLLKPV
>Mature_415_residues
TTQTFRSDFLRTLHERGYIHQCSDLERLDAKAAEGPITAYIGFDATASSLHAGHLLSIMMLRTLQRTGHNPIALMGGGTT
KIGDPSGKDEARKMLTDQQIEDNIASIRKVFARFLDFGAGAKMENNASWLDELKYIPLLREVGPHFTINRMLTFDSVKLR
LEREQPLTFLEFNYMILQAYDFVELNARHGCILQMGGSDQWGNIVNGMELGRRMRGADLFALTTPLLTTSSGAKMGKTAG
GAVWLDADLLSPYEYWQYWRNAGDADVERFLKLFTELPLDEIARLAALEGQEINHAKEVLATEATALLHGREAAEKAKET
SRATFADGALAVDLPTVEVPRATLEAGLPVAKAFVDAGLVASTSEARRQIKGGGLKVNDVTVTDEKAVIDLGALTPEGVV
KLSLGKKKHVLLKPV

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Homo sapiens, GI94681057, Length=413, Percent_Identity=36.319612590799, Blast_Score=249, Evalue=2e-66,
Organism=Escherichia coli, GI1787925, Length=393, Percent_Identity=42.2391857506361, Blast_Score=321, Evalue=5e-89,
Organism=Caenorhabditis elegans, GI25145802, Length=325, Percent_Identity=34.4615384615385, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI25145805, Length=217, Percent_Identity=35.0230414746544, Blast_Score=119, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6325160, Length=445, Percent_Identity=32.5842696629214, Blast_Score=217, Evalue=3e-57,
Organism=Drosophila melanogaster, GI20130383, Length=435, Percent_Identity=35.8620689655172, Blast_Score=248, Evalue=5e-66,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYY_XANP2 (A7INU9)

Other databases:

- EMBL:   CP000781
- RefSeq:   YP_001419352.1
- ProteinModelPortal:   A7INU9
- SMR:   A7INU9
- STRING:   A7INU9
- GeneID:   5421987
- GenomeReviews:   CP000781_GR
- KEGG:   xau:Xaut_4474
- eggNOG:   COG0162
- HOGENOM:   HBG288125
- OMA:   TFYIGFD
- ProtClustDB:   PRK05912
- BioCyc:   XAUT78245:XAUT_4474-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_02006
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11766
- PRINTS:   PR01040
- SMART:   SM00363
- TIGRFAMs:   TIGR00234

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b

EC number: =6.1.1.1

Molecular weight: Translated: 45626; Mature: 45495

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50889 S4

Important sites: BINDING 41-41 BINDING 175-175 BINDING 179-179 BINDING 238-238

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQTFRSDFLRTLHERGYIHQCSDLERLDAKAAEGPITAYIGFDATASSLHAGHLLSIM
CCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHH
MLRTLQRTGHNPIALMGGGTTKIGDPSGKDEARKMLTDQQIEDNIASIRKVFARFLDFGA
HHHHHHHCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GAKMENNASWLDELKYIPLLREVGPHFTINRMLTFDSVKLRLEREQPLTFLEFNYMILQA
CCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEECCEEEEEECCCCCEEEECCEEEEEE
YDFVELNARHGCILQMGGSDQWGNIVNGMELGRRMRGADLFALTTPLLTTSSGAKMGKTA
ECEEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEECCCCEECCCCCCCCCCC
GGAVWLDADLLSPYEYWQYWRNAGDADVERFLKLFTELPLDEIARLAALEGQEINHAKEV
CCEEEEEHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHH
LATEATALLHGREAAEKAKETSRATFADGALAVDLPTVEVPRATLEAGLPVAKAFVDAGL
HHHHHHHHHHCHHHHHHHHHHCCCEECCCEEEEECCCCCCCHHHHHHCCHHHHHHHHCCC
VASTSEARRQIKGGGLKVNDVTVTDEKAVIDLGALTPEGVVKLSLGKKKHVLLKPV
CCCCHHHHHHHCCCCEEEEEEEEECCCEEEEECCCCCCCEEEEECCCCCCEEECCC
>Mature Secondary Structure 
TTQTFRSDFLRTLHERGYIHQCSDLERLDAKAAEGPITAYIGFDATASSLHAGHLLSIM
CCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHH
MLRTLQRTGHNPIALMGGGTTKIGDPSGKDEARKMLTDQQIEDNIASIRKVFARFLDFGA
HHHHHHHCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GAKMENNASWLDELKYIPLLREVGPHFTINRMLTFDSVKLRLEREQPLTFLEFNYMILQA
CCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEECCEEEEEECCCCCEEEECCEEEEEE
YDFVELNARHGCILQMGGSDQWGNIVNGMELGRRMRGADLFALTTPLLTTSSGAKMGKTA
ECEEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEECCCCEECCCCCCCCCCC
GGAVWLDADLLSPYEYWQYWRNAGDADVERFLKLFTELPLDEIARLAALEGQEINHAKEV
CCEEEEEHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHH
LATEATALLHGREAAEKAKETSRATFADGALAVDLPTVEVPRATLEAGLPVAKAFVDAGL
HHHHHHHHHHCHHHHHHHHHHCCCEECCCEEEEECCCCCCCHHHHHHCCHHHHHHHHCCC
VASTSEARRQIKGGGLKVNDVTVTDEKAVIDLGALTPEGVVKLSLGKKKHVLLKPV
CCCCHHHHHHHCCCCEEEEEEEEECCCEEEEECCCCCCCEEEEECCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA