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The map label for this gene is ycdT [H]

Identifier: 154248337

GI number: 154248337

Start: 4897984

End: 4899189

Strand: Reverse

Name: ycdT [H]

Synonym: Xaut_4417

Alternate gene names: 154248337

Gene position: 4899189-4897984 (Counterclockwise)

Preceding gene: 154248338

Following gene: 154248334

Centisome position: 92.28

GC content: 67.66

Gene sequence:

>1206_bases
GTGTACGGACGACGCCGCATGCACGCCGCCGCGTTTACACTGTTCATGAACGTTGCCGTCGCGGGGCTCTTTGCGGCGAG
CTACGCGACAATCGCCTTAATGCATCCGGCGCAGCGGGCACCGATCTGGTTCGCGGTCTGCTATGGGCTGGGCATGGTGA
CGCCGCTGGCGCAGTTCGGTCTCGCCTACACCGCCTGGACCACCTTTTTCGGCGCCGTCATCTTTCTCAGCCTCGCCTTC
GCGCTTCTGCTGCTGGTGCCGGCGCTTGCGGTCTTCTACGGCAGCCGCAAGCCATGGCCGGCGGTGGGGGCAATCGCGGC
GCTCACCCTGCTCCTGCCGGTGATCCGCGCGGGCCTGCCCTACCATCCCCTGACCTACCAGTCGATGTATCAGGCTCCGT
TCACGCTCGCGGTCGCCGCCTGCGCCTGGGTGGTGCTGCGCGACAGTCCTCGCCACCGCAGCGATTTCGTGCTTGCCGGG
ATGTTGGTGGTATTGGCCGCGCACTTCCCGGTGAAGGCCTATCTGCTGGTACAGATGGGCACGTCGCGCACGGCGAAATA
TATCGACACCACCTATGCCCTCGTCTCGCAGGTCAGCTCTGGCATCCTGTTCCTGGCCACCGGGCTGATCCTGCTGCTGA
AGTCGGTGCAGGCCGTGGTCCGTGAAAGCCAAATTGCTGCGGAGACCGACGTGTTGTCCGGCCTGCTGAACCGGCGCGGC
TTCGACAGCAGGGCCGAGCGCCTCATCGCCCAGGCCAAGGGGCGGCCGGTGGCGCTCCTCATCCTCGACCTGGACCACTT
CAAGAGTGTCAACGACAGGTTCGGCCACAGCACCGGAGATGCCGCCATCCGGGCCTTCGCAGTGCTGCTTTCGCGCACTG
TGCCCCCCTCTGCTCTGATTGCCCGCCTCGGCGGCGAGGAGTTCGCCGTGCTCCTCGATCGCACCGGGCTGGAGGCCGCC
CGGCTCCAGGCGGAGGCGATCCGACTCGCCACGTTCCACCATCAAGAAGTGGGCCTGCCGAAGCTGACGGTGAGCATCGG
CGTCGCGGCCCCTGCCTCGTCCCACCTCCTGCCGGAGGCCATGGAGCGGGCGGACGCCGCGCTCTACGAAGCCAAGCGCG
CCGGCCGGAACCGGGTCTGCTGCGCGGCGGAAAAGACGGCGCCGGCGGTTCCCCTGGCCGTAGTGGCGCAGCGCAACGCG
ACCTGA

Upstream 100 bases:

>100_bases
GCCAAGTCGGTGACGGTGGAATAGGCTGAATGGTGCATCGCTCCGCGGCGCGCCCGCCGCTGACGTCAAAGTCGCATCGT
GCTTATGGTGTGCGCTCACC

Downstream 100 bases:

>100_bases
CGCCTTTCCGCAATCACCGGTTCGGCCGGGTGGCGATAAATACCGCGAGGGCGATGAACACCACGCCCAGCACCGCCAGC
ACCGGCACCGGCGCGCCGAA

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MYGRRRMHAAAFTLFMNVAVAGLFAASYATIALMHPAQRAPIWFAVCYGLGMVTPLAQFGLAYTAWTTFFGAVIFLSLAF
ALLLLVPALAVFYGSRKPWPAVGAIAALTLLLPVIRAGLPYHPLTYQSMYQAPFTLAVAACAWVVLRDSPRHRSDFVLAG
MLVVLAAHFPVKAYLLVQMGTSRTAKYIDTTYALVSQVSSGILFLATGLILLLKSVQAVVRESQIAAETDVLSGLLNRRG
FDSRAERLIAQAKGRPVALLILDLDHFKSVNDRFGHSTGDAAIRAFAVLLSRTVPPSALIARLGGEEFAVLLDRTGLEAA
RLQAEAIRLATFHHQEVGLPKLTVSIGVAAPASSHLLPEAMERADAALYEAKRAGRNRVCCAAEKTAPAVPLAVVAQRNA
T

Sequences:

>Translated_401_residues
MYGRRRMHAAAFTLFMNVAVAGLFAASYATIALMHPAQRAPIWFAVCYGLGMVTPLAQFGLAYTAWTTFFGAVIFLSLAF
ALLLLVPALAVFYGSRKPWPAVGAIAALTLLLPVIRAGLPYHPLTYQSMYQAPFTLAVAACAWVVLRDSPRHRSDFVLAG
MLVVLAAHFPVKAYLLVQMGTSRTAKYIDTTYALVSQVSSGILFLATGLILLLKSVQAVVRESQIAAETDVLSGLLNRRG
FDSRAERLIAQAKGRPVALLILDLDHFKSVNDRFGHSTGDAAIRAFAVLLSRTVPPSALIARLGGEEFAVLLDRTGLEAA
RLQAEAIRLATFHHQEVGLPKLTVSIGVAAPASSHLLPEAMERADAALYEAKRAGRNRVCCAAEKTAPAVPLAVVAQRNA
T
>Mature_401_residues
MYGRRRMHAAAFTLFMNVAVAGLFAASYATIALMHPAQRAPIWFAVCYGLGMVTPLAQFGLAYTAWTTFFGAVIFLSLAF
ALLLLVPALAVFYGSRKPWPAVGAIAALTLLLPVIRAGLPYHPLTYQSMYQAPFTLAVAACAWVVLRDSPRHRSDFVLAG
MLVVLAAHFPVKAYLLVQMGTSRTAKYIDTTYALVSQVSSGILFLATGLILLLKSVQAVVRESQIAAETDVLSGLLNRRG
FDSRAERLIAQAKGRPVALLILDLDHFKSVNDRFGHSTGDAAIRAFAVLLSRTVPPSALIARLGGEEFAVLLDRTGLEAA
RLQAEAIRLATFHHQEVGLPKLTVSIGVAAPASSHLLPEAMERADAALYEAKRAGRNRVCCAAEKTAPAVPLAVVAQRNA
T

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=211, Percent_Identity=35.0710900473934, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI87081881, Length=165, Percent_Identity=43.030303030303, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI1786584, Length=169, Percent_Identity=39.6449704142012, Blast_Score=105, Evalue=7e-24,
Organism=Escherichia coli, GI1787802, Length=173, Percent_Identity=34.1040462427746, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI145693134, Length=183, Percent_Identity=34.9726775956284, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1788381, Length=191, Percent_Identity=32.9842931937173, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1787816, Length=166, Percent_Identity=34.3373493975904, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI87082007, Length=165, Percent_Identity=36.969696969697, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI1788085, Length=173, Percent_Identity=28.9017341040462, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1787541, Length=165, Percent_Identity=33.9393939393939, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI87081974, Length=213, Percent_Identity=30.5164319248826, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1787056, Length=151, Percent_Identity=31.1258278145695, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI87081977, Length=193, Percent_Identity=30.5699481865285, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1788956, Length=153, Percent_Identity=32.6797385620915, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 43066; Mature: 43066

Theoretical pI: Translated: 10.24; Mature: 10.24

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHCCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]