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The map label for this gene is tpiA

Identifier: 154248300

GI number: 154248300

Start: 4856997

End: 4857761

Strand: Direct

Name: tpiA

Synonym: Xaut_4380

Alternate gene names: 154248300

Gene position: 4856997-4857761 (Clockwise)

Preceding gene: 154248290

Following gene: 154248302

Centisome position: 91.49

GC content: 69.67

Gene sequence:

>765_bases
ATGCCCGCTTCCCGCCGCGTCCTGATCGCCGGCAACTGGAAAATGAACGGGCTGAAGAGCTCCCTCGCCGAGATCGAGGC
CCTGGCCGCGGGCTATGATGGCGACCTCAAGGCCAAGCTGGACCTGCTGGTCTGCCCGCCCGCAACGCTCCTGATCTCCG
CCGCCGAGCGCCTCGCCGGCTCCGGGGTGCAGCTGGGCGGCCAGAATTGCCATCCGGCCCCCGCGGGCGCCCACACCGGC
GGCATTTCCGCCGAGATGCTGAAGGATGCCGGCGTCACGTCGGTCATCGTCGGCCATTCCGAGCGTCGCACCAACCTGTC
GGAGAGCGATGCGGTGGTGATGGCCAAGGTGAAGGCGGCGTGGTGCTTCGGGTTGCTGCCCATCGTCTGCGTCGGCGAGA
CCGCCGAGGAGCGCGATGCCGGCGAGGCCGTGGGGGTGGTGACGCGCCAGGTGCACCAGTCGCTGCCGGAAGGCTGCACC
GCATCCAACCTGGTCATCGCCTACGAGCCGGTCTGGGCCATCGGCACCGGCCGCACGCCCACCCCTGAGGACGTGGCCAA
CATCCATGGCACCATCCGCTCCGTGCTCCGCTCACAGTTCGGCGCCGAGGCTGACGGTATTCGCATCCTCTACGGCGGCT
CGGTGAAGCCAGACAATGCGGCGACCCTGATGGCCGTTGCCGATGTGGACGGCGCACTGGTGGGAGGCGCCAGCCTGAAG
GCAGCGGATTTCCTCGCCATCGCCCGGGCCACCCCGGCGCGGTGA

Upstream 100 bases:

>100_bases
GAGGCCATCCTGCTCCCGCAACGGCGCATCTTGATGCCTTTTGGCCGGGAAAGGGCTCTGATAGAGCGAACCCCGATCAA
CCTTAAAGGAGAGCCGAACC

Downstream 100 bases:

>100_bases
GAAGGCGGCGCGGCGGAGCACGAAGTCTCCCCCGCGCCGCATCAAAGGCCACGGTCGATTGGAAGGCCGCTCGCGACGGC
GTTCGCGGAACCCGGATATC

Product: Triose-phosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MPASRRVLIAGNWKMNGLKSSLAEIEALAAGYDGDLKAKLDLLVCPPATLLISAAERLAGSGVQLGGQNCHPAPAGAHTG
GISAEMLKDAGVTSVIVGHSERRTNLSESDAVVMAKVKAAWCFGLLPIVCVGETAEERDAGEAVGVVTRQVHQSLPEGCT
ASNLVIAYEPVWAIGTGRTPTPEDVANIHGTIRSVLRSQFGAEADGIRILYGGSVKPDNAATLMAVADVDGALVGGASLK
AADFLAIARATPAR

Sequences:

>Translated_254_residues
MPASRRVLIAGNWKMNGLKSSLAEIEALAAGYDGDLKAKLDLLVCPPATLLISAAERLAGSGVQLGGQNCHPAPAGAHTG
GISAEMLKDAGVTSVIVGHSERRTNLSESDAVVMAKVKAAWCFGLLPIVCVGETAEERDAGEAVGVVTRQVHQSLPEGCT
ASNLVIAYEPVWAIGTGRTPTPEDVANIHGTIRSVLRSQFGAEADGIRILYGGSVKPDNAATLMAVADVDGALVGGASLK
AADFLAIARATPAR
>Mature_253_residues
PASRRVLIAGNWKMNGLKSSLAEIEALAAGYDGDLKAKLDLLVCPPATLLISAAERLAGSGVQLGGQNCHPAPAGAHTGG
ISAEMLKDAGVTSVIVGHSERRTNLSESDAVVMAKVKAAWCFGLLPIVCVGETAEERDAGEAVGVVTRQVHQSLPEGCTA
SNLVIAYEPVWAIGTGRTPTPEDVANIHGTIRSVLRSQFGAEADGIRILYGGSVKPDNAATLMAVADVDGALVGGASLKA
ADFLAIARATPAR

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI4507645, Length=253, Percent_Identity=42.6877470355731, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI226529917, Length=253, Percent_Identity=42.6877470355731, Blast_Score=172, Evalue=2e-43,
Organism=Escherichia coli, GI1790353, Length=253, Percent_Identity=42.2924901185771, Blast_Score=172, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17536593, Length=249, Percent_Identity=45.7831325301205, Blast_Score=197, Evalue=5e-51,
Organism=Saccharomyces cerevisiae, GI6320255, Length=239, Percent_Identity=44.7698744769874, Blast_Score=194, Evalue=1e-50,
Organism=Drosophila melanogaster, GI28572008, Length=248, Percent_Identity=43.5483870967742, Blast_Score=177, Evalue=6e-45,
Organism=Drosophila melanogaster, GI28572006, Length=248, Percent_Identity=43.5483870967742, Blast_Score=177, Evalue=6e-45,
Organism=Drosophila melanogaster, GI28572004, Length=248, Percent_Identity=43.5483870967742, Blast_Score=177, Evalue=7e-45,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_XANP2 (A7INK7)

Other databases:

- EMBL:   CP000781
- RefSeq:   YP_001419258.1
- ProteinModelPortal:   A7INK7
- SMR:   A7INK7
- STRING:   A7INK7
- GeneID:   5422965
- GenomeReviews:   CP000781_GR
- KEGG:   xau:Xaut_4380
- eggNOG:   COG0149
- HOGENOM:   HBG708281
- OMA:   DIRSVQT
- BioCyc:   XAUT78245:XAUT_4380-MONOMER
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 26029; Mature: 25898

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 99-99 ACT_SITE 169-169 BINDING 12-12 BINDING 14-14

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPASRRVLIAGNWKMNGLKSSLAEIEALAAGYDGDLKAKLDLLVCPPATLLISAAERLAG
CCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCHHHHHHHHHHHCC
SGVQLGGQNCHPAPAGAHTGGISAEMLKDAGVTSVIVGHSERRTNLSESDAVVMAKVKAA
CCEEECCCCCCCCCCCCCCCCCHHHHHHHCCCEEEEECCCHHCCCCCCCCCEEEEHHHHH
WCFGLLPIVCVGETAEERDAGEAVGVVTRQVHQSLPEGCTASNLVIAYEPVWAIGTGRTP
HHHHHHHHEEECCCHHHHCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCEEEECCCCCC
TPEDVANIHGTIRSVLRSQFGAEADGIRILYGGSVKPDNAATLMAVADVDGALVGGASLK
CHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEEEEEECCCCEEECCCCCC
AADFLAIARATPAR
HHHHHHHHCCCCCC
>Mature Secondary Structure 
PASRRVLIAGNWKMNGLKSSLAEIEALAAGYDGDLKAKLDLLVCPPATLLISAAERLAG
CCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCHHHHHHHHHHHCC
SGVQLGGQNCHPAPAGAHTGGISAEMLKDAGVTSVIVGHSERRTNLSESDAVVMAKVKAA
CCEEECCCCCCCCCCCCCCCCCHHHHHHHCCCEEEEECCCHHCCCCCCCCCEEEEHHHHH
WCFGLLPIVCVGETAEERDAGEAVGVVTRQVHQSLPEGCTASNLVIAYEPVWAIGTGRTP
HHHHHHHHEEECCCHHHHCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCEEEECCCCCC
TPEDVANIHGTIRSVLRSQFGAEADGIRILYGGSVKPDNAATLMAVADVDGALVGGASLK
CHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEEEEEECCCCEEECCCCCC
AADFLAIARATPAR
HHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA