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The map label for this gene is hipO [H]

Identifier: 154248287

GI number: 154248287

Start: 4842042

End: 4843208

Strand: Direct

Name: hipO [H]

Synonym: Xaut_4367

Alternate gene names: 154248287

Gene position: 4842042-4843208 (Clockwise)

Preceding gene: 154248284

Following gene: 154248290

Centisome position: 91.21

GC content: 70.69

Gene sequence:

>1167_bases
ATGCCCGTCCATAATCACATTGCCTCCGGCGCCGACGAGATCGCGGCCTGGCGCCAGGATTTCCATCGCCACCCGGAGCT
CATGTACGAGCTGCCCCGCACCAGCGCCGCAGTGGCCGACAAGCTGCGTGCCTTCGGCTGCGACGAGGTGATCACCGGGC
TCGGGCGCACCGGCGTGGTCGGCATCGTGCGCGGACGCGGGGACGGGCCCATGATCGGGCTGCGGGCCGACATGGACGCT
CTGCCCATCACTGAGCAGACGGGCGCCGCCTATGCCTCGCAGGCCCCCGGCGTCATGCATGCCTGCGGCCATGACGGCCA
CACCGCCATGCTGCTCGGAGCGGCGCGCCACCTCGCGGAAACCCGCGCCTTCTCCGGCACCGCCGTGCTCATCTTCCAGC
CGGCGGAGGAGGGCGGGGCCGGCGCCAAGGCCATGATCGACGACGGCCTGTTCGCTCGCTTCCCGGTGAGCGAGGTCTAT
GGCATGCACAATCTGCCGGGGCTGCCCGTGGGCCGCTTCGCCATGCGGCAGGGCGGTATCATGGCCTCGTCCGACCGCAT
CGAGATCCTGGTGGACGGCCGCGGCGCCCATGCCGCGCGGCCCAACCAGGGGGTGGACGTCGTGCTCACCGGTGCCGCCA
TCGTCACCGCCCTTCAGCATGTGGTGTCGCGCAATCTCGATCCGCTGGCCGCCGCGGTCATCTCCATTCCCATGTTCCAT
GCGGGGGAGGCGGACAATGTGCTGCCGCCCAGCGCGCGGCTCGCCGGTACCATGCGCGCCCTCGATCCCCATGTGCGCGA
GGTGCTGCGCGCCCGCATCGTGGCGGTGGCGGAAGGGGTGGCGGCGGCCCACGGCGCCACGGCGACGGTGAGCTTTTCCC
AGGGCTATCCCGTCACCCGCAACCACGCGGCGCAGACCGCGTTCGCGGCCGAGGTCGCAGCCGACGTGGCCGGCCTGTCG
GAGGTGGACGCCGCCGCCCCGCCGCTGATGGCGGCGGAGGATTTCGCCTTCATGCTGGAGGAAAAGCCCGGCGCCTACAT
CTTCATCGGCAACGGCCCGTCCGCCGACCTGCACCATCCGGAATACGACTTCGCGGATACCGCCATTCCGTTCGGTGCGT
CGTATTGGGTGCGGTTGGTGGAACGGGCTCTGCCGCTCTCGGTCTGA

Upstream 100 bases:

>100_bases
GGAAAGATGCGCGGGGTGGCGTGGGCTGTGCATATCCCAAGCCCATTGACCGGCAGGCGGCCCGTGCCCACCATGGCCCC
TCATTGCTCCTGGAATTGCC

Downstream 100 bases:

>100_bases
GCCGGTACCGGGCTTTCAGCCCGGAAACGTCAGCGGTTGCCGGGCCTTCAGCCCGGCTTGCGCAGGACGAAGGTGAGAAG
GCCTTCCGCCACCGTGCGCT

Product: amidohydrolase

Products: NA

Alternate protein names: Benzoylglycine amidohydrolase; Hippuricase [H]

Number of amino acids: Translated: 388; Mature: 387

Protein sequence:

>388_residues
MPVHNHIASGADEIAAWRQDFHRHPELMYELPRTSAAVADKLRAFGCDEVITGLGRTGVVGIVRGRGDGPMIGLRADMDA
LPITEQTGAAYASQAPGVMHACGHDGHTAMLLGAARHLAETRAFSGTAVLIFQPAEEGGAGAKAMIDDGLFARFPVSEVY
GMHNLPGLPVGRFAMRQGGIMASSDRIEILVDGRGAHAARPNQGVDVVLTGAAIVTALQHVVSRNLDPLAAAVISIPMFH
AGEADNVLPPSARLAGTMRALDPHVREVLRARIVAVAEGVAAAHGATATVSFSQGYPVTRNHAAQTAFAAEVAADVAGLS
EVDAAAPPLMAAEDFAFMLEEKPGAYIFIGNGPSADLHHPEYDFADTAIPFGASYWVRLVERALPLSV

Sequences:

>Translated_388_residues
MPVHNHIASGADEIAAWRQDFHRHPELMYELPRTSAAVADKLRAFGCDEVITGLGRTGVVGIVRGRGDGPMIGLRADMDA
LPITEQTGAAYASQAPGVMHACGHDGHTAMLLGAARHLAETRAFSGTAVLIFQPAEEGGAGAKAMIDDGLFARFPVSEVY
GMHNLPGLPVGRFAMRQGGIMASSDRIEILVDGRGAHAARPNQGVDVVLTGAAIVTALQHVVSRNLDPLAAAVISIPMFH
AGEADNVLPPSARLAGTMRALDPHVREVLRARIVAVAEGVAAAHGATATVSFSQGYPVTRNHAAQTAFAAEVAADVAGLS
EVDAAAPPLMAAEDFAFMLEEKPGAYIFIGNGPSADLHHPEYDFADTAIPFGASYWVRLVERALPLSV
>Mature_387_residues
PVHNHIASGADEIAAWRQDFHRHPELMYELPRTSAAVADKLRAFGCDEVITGLGRTGVVGIVRGRGDGPMIGLRADMDAL
PITEQTGAAYASQAPGVMHACGHDGHTAMLLGAARHLAETRAFSGTAVLIFQPAEEGGAGAKAMIDDGLFARFPVSEVYG
MHNLPGLPVGRFAMRQGGIMASSDRIEILVDGRGAHAARPNQGVDVVLTGAAIVTALQHVVSRNLDPLAAAVISIPMFHA
GEADNVLPPSARLAGTMRALDPHVREVLRARIVAVAEGVAAAHGATATVSFSQGYPVTRNHAAQTAFAAEVAADVAGLSE
VDAAAPPLMAAEDFAFMLEEKPGAYIFIGNGPSADLHHPEYDFADTAIPFGASYWVRLVERALPLSV

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=419, Percent_Identity=26.7303102625298, Blast_Score=105, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.32 [H]

Molecular weight: Translated: 40603; Mature: 40471

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVHNHIASGADEIAAWRQDFHRHPELMYELPRTSAAVADKLRAFGCDEVITGLGRTGVV
CCCCHHHHCCHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHCCHHHHHHCCCCCCEE
GIVRGRGDGPMIGLRADMDALPITEQTGAAYASQAPGVMHACGHDGHTAMLLGAARHLAE
EEEEECCCCCEEEEECCCCCCCCCHHCCCHHHHCCCCCEECCCCCCCHHHHHHHHHHHHH
TRAFSGTAVLIFQPAEEGGAGAKAMIDDGLFARFPVSEVYGMHNLPGLPVGRFAMRQGGI
HHCCCCCEEEEEECCCCCCCCCEEECCCCCEEECCHHHHCCCCCCCCCCHHHHHHHCCCE
MASSDRIEILVDGRGAHAARPNQGVDVVLTGAAIVTALQHVVSRNLDPLAAAVISIPMFH
EECCCCEEEEEECCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHCCCCHHHHHHHHCCCCC
AGEADNVLPPSARLAGTMRALDPHVREVLRARIVAVAEGVAAAHGATATVSFSQGYPVTR
CCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCC
NHAAQTAFAAEVAADVAGLSEVDAAAPPLMAAEDFAFMLEEKPGAYIFIGNGPSADLHHP
CCHHHHHHHHHHHHHHHCCHHHHCCCCCCCCHHHHHHHEECCCCEEEEECCCCCCCCCCC
EYDFADTAIPFGASYWVRLVERALPLSV
CCCCCCCCCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PVHNHIASGADEIAAWRQDFHRHPELMYELPRTSAAVADKLRAFGCDEVITGLGRTGVV
CCCHHHHCCHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHCCHHHHHHCCCCCCEE
GIVRGRGDGPMIGLRADMDALPITEQTGAAYASQAPGVMHACGHDGHTAMLLGAARHLAE
EEEEECCCCCEEEEECCCCCCCCCHHCCCHHHHCCCCCEECCCCCCCHHHHHHHHHHHHH
TRAFSGTAVLIFQPAEEGGAGAKAMIDDGLFARFPVSEVYGMHNLPGLPVGRFAMRQGGI
HHCCCCCEEEEEECCCCCCCCCEEECCCCCEEECCHHHHCCCCCCCCCCHHHHHHHCCCE
MASSDRIEILVDGRGAHAARPNQGVDVVLTGAAIVTALQHVVSRNLDPLAAAVISIPMFH
EECCCCEEEEEECCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHCCCCHHHHHHHHCCCCC
AGEADNVLPPSARLAGTMRALDPHVREVLRARIVAVAEGVAAAHGATATVSFSQGYPVTR
CCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCC
NHAAQTAFAAEVAADVAGLSEVDAAAPPLMAAEDFAFMLEEKPGAYIFIGNGPSADLHHP
CCHHHHHHHHHHHHHHHCCHHHHCCCCCCCCHHHHHHHEECCCCEEEEECCCCCCCCCCC
EYDFADTAIPFGASYWVRLVERALPLSV
CCCCCCCCCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7730270; 10688204 [H]