Click here to switch to the map view.

The map label for this gene is catB1 [H]

Identifier: 154248278

GI number: 154248278

Start: 4831426

End: 4832601

Strand: Reverse

Name: catB1 [H]

Synonym: Xaut_4358

Alternate gene names: 154248278

Gene position: 4832601-4831426 (Counterclockwise)

Preceding gene: 154248280

Following gene: 154248277

Centisome position: 91.03

GC content: 71.09

Gene sequence:

>1176_bases
ATGCAGACCGGCGTCGCAGTGCGTATCGACCGTATCGAGACCTTTATCGTCGATATCCCAACCATACGCGCCCATCACCT
GTCCATGCACACCCTGCGCAGCCAGACGCTGGTGCTGGTGAAAGTGTCGTGCTCGGACGGCACGGTCGGCGTCGGCGAGG
GCACCACCATCGGCGGCCTGAGCTATGGCGAGGAGAGCCCGGAAGGCATCAAGCTCACCATCGACACCTATATCGCCCCC
CTGCTGCTCGGCGCCGACGCCACCCGGGTGGCCGCGCTGATGGCCAGCGTGAAGCGGGCCGTGAAGGGCAACCGCTTCGC
CCGCTGCGCGGTGGAGACCGCCCTTCTCGATGTCCACGGGCGCCGCCTCGGGGTGCCGGTGTCGGAGTTCTTCGGCGGTC
GCCAGCGCGACACGCTGGAGGTGGCCTGGACCCTCGCCAGCGGCGACACCGCGACGGACATCGAGGAGGCCGAGGCCATG
CTGGCGGCCCGCCGCCACCGCATCTTCAAGCTGAAGATCGGCAAGCGCGAGGTGGCAGCGGACGTGGCCCATGTGGCCGC
CATCAAGGCGGCCCTGGGCGACCGTGCCAGCGTGCGGGTGGACGTGAACATGGCCTGGAGCGAACATCAGGCCCGTCGCG
GCATCGCCGCGCTGGAGGCGGCCGGGGTCGATCTCATCGAGCAGCCGGTTAGCCGGGAGCAGCGCGGCGCCCTCGCCCGC
CTGACGCGCCGGTTCGACGCGGCCATCATGGCCGACGAAGCCCTTCAGGGCCCGGCCGAGGCCATGGATCTGGCGCGCGA
CCGTGCCGCCGGGGTGTTCGCGCTGAAGATCGCCCAGTCGGGCGGCCTGTTCGAGGCCCAGCGGGTGGCGGCCATCGCCG
ACGCGGCGGGCATCGAGCTTTATGGCGGCACCATGCTGGAAGGTGGCATCGGCACCGCGGCGTCGGCCCAGCTCTGCGCC
ACCCTGCCGACGCTCACCTGGGGCACCGAGCTGTTCGGCCCCCTGCTACTGGCCGAGGAGATCCTGGCGACGCCGCTCGC
CTATGCCGATTTCGCCCTGATCGTTCCGAGCACGCCGGGCCTCGGCGTGGAGCTGGACATGGACCGGGTGGACTTCTTCC
GCCGCGACCGCCCGGCCCCCCGCCTCAGCCCCGTTCGCCAGTCCGTGGGGGCCTGA

Upstream 100 bases:

>100_bases
ATATCCAGACCTATTAGGTATGCAAACCGACCGAGACTGTCTTTGACGCGCGCCCCGTCGGCGTGATTTTCCTTATGCCG
AACATAAGGACACTCCACGG

Downstream 100 bases:

>100_bases
GACCCCCTGCCCTTTCGACCGATGCCCCAGCGCACGGCGCATAAAAATGCCCCATAAGGAGAACGCCCATGGAAAAAGCC
GCCATCGATGCCCTCTTGAA

Product: muconate and chloromuconate cycloisomerase

Products: NA

Alternate protein names: Cis,cis-muconate lactonizing enzyme I 1; MLE 1; Muconate cycloisomerase I 1 [H]

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MQTGVAVRIDRIETFIVDIPTIRAHHLSMHTLRSQTLVLVKVSCSDGTVGVGEGTTIGGLSYGEESPEGIKLTIDTYIAP
LLLGADATRVAALMASVKRAVKGNRFARCAVETALLDVHGRRLGVPVSEFFGGRQRDTLEVAWTLASGDTATDIEEAEAM
LAARRHRIFKLKIGKREVAADVAHVAAIKAALGDRASVRVDVNMAWSEHQARRGIAALEAAGVDLIEQPVSREQRGALAR
LTRRFDAAIMADEALQGPAEAMDLARDRAAGVFALKIAQSGGLFEAQRVAAIADAAGIELYGGTMLEGGIGTAASAQLCA
TLPTLTWGTELFGPLLLAEEILATPLAYADFALIVPSTPGLGVELDMDRVDFFRRDRPAPRLSPVRQSVGA

Sequences:

>Translated_391_residues
MQTGVAVRIDRIETFIVDIPTIRAHHLSMHTLRSQTLVLVKVSCSDGTVGVGEGTTIGGLSYGEESPEGIKLTIDTYIAP
LLLGADATRVAALMASVKRAVKGNRFARCAVETALLDVHGRRLGVPVSEFFGGRQRDTLEVAWTLASGDTATDIEEAEAM
LAARRHRIFKLKIGKREVAADVAHVAAIKAALGDRASVRVDVNMAWSEHQARRGIAALEAAGVDLIEQPVSREQRGALAR
LTRRFDAAIMADEALQGPAEAMDLARDRAAGVFALKIAQSGGLFEAQRVAAIADAAGIELYGGTMLEGGIGTAASAQLCA
TLPTLTWGTELFGPLLLAEEILATPLAYADFALIVPSTPGLGVELDMDRVDFFRRDRPAPRLSPVRQSVGA
>Mature_391_residues
MQTGVAVRIDRIETFIVDIPTIRAHHLSMHTLRSQTLVLVKVSCSDGTVGVGEGTTIGGLSYGEESPEGIKLTIDTYIAP
LLLGADATRVAALMASVKRAVKGNRFARCAVETALLDVHGRRLGVPVSEFFGGRQRDTLEVAWTLASGDTATDIEEAEAM
LAARRHRIFKLKIGKREVAADVAHVAAIKAALGDRASVRVDVNMAWSEHQARRGIAALEAAGVDLIEQPVSREQRGALAR
LTRRFDAAIMADEALQGPAEAMDLARDRAAGVFALKIAQSGGLFEAQRVAAIADAAGIELYGGTMLEGGIGTAASAQLCA
TLPTLTWGTELFGPLLLAEEILATPLAYADFALIVPSTPGLGVELDMDRVDFFRRDRPAPRLSPVRQSVGA

Specific function: Catalyzes a syn cycloisomerization [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI48994953, Length=373, Percent_Identity=26.2734584450402, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013370
- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =5.5.1.1 [H]

Molecular weight: Translated: 41524; Mature: 41524

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTGVAVRIDRIETFIVDIPTIRAHHLSMHTLRSQTLVLVKVSCSDGTVGVGEGTTIGGL
CCCCEEEEEECEEEEEEECCCHHHHHHHHHHHCCCEEEEEEEECCCCEEECCCCCEECCC
SYGEESPEGIKLTIDTYIAPLLLGADATRVAALMASVKRAVKGNRFARCAVETALLDVHG
CCCCCCCCCEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCC
RRLGVPVSEFFGGRQRDTLEVAWTLASGDTATDIEEAEAMLAARRHRIFKLKIGKREVAA
CCCCCCHHHHCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHCEEEEEEECCHHHHH
DVAHVAAIKAALGDRASVRVDVNMAWSEHQARRGIAALEAAGVDLIEQPVSREQRGALAR
HHHHHHHHHHHHCCCCEEEEEEEECCHHHHHHCCHHHHHHCCHHHHHHHHCHHHHHHHHH
LTRRFDAAIMADEALQGPAEAMDLARDRAAGVFALKIAQSGGLFEAQRVAAIADAAGIEL
HHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHCCCEEE
YGGTMLEGGIGTAASAQLCATLPTLTWGTELFGPLLLAEEILATPLAYADFALIVPSTPG
ECCEEEECCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHCCEEEEEECCCC
LGVELDMDRVDFFRRDRPAPRLSPVRQSVGA
CCEEECHHHHHHHHCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MQTGVAVRIDRIETFIVDIPTIRAHHLSMHTLRSQTLVLVKVSCSDGTVGVGEGTTIGGL
CCCCEEEEEECEEEEEEECCCHHHHHHHHHHHCCCEEEEEEEECCCCEEECCCCCEECCC
SYGEESPEGIKLTIDTYIAPLLLGADATRVAALMASVKRAVKGNRFARCAVETALLDVHG
CCCCCCCCCEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCC
RRLGVPVSEFFGGRQRDTLEVAWTLASGDTATDIEEAEAMLAARRHRIFKLKIGKREVAA
CCCCCCHHHHCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHCEEEEEEECCHHHHH
DVAHVAAIKAALGDRASVRVDVNMAWSEHQARRGIAALEAAGVDLIEQPVSREQRGALAR
HHHHHHHHHHHHCCCCEEEEEEEECCHHHHHHCCHHHHHHCCHHHHHHHHCHHHHHHHHH
LTRRFDAAIMADEALQGPAEAMDLARDRAAGVFALKIAQSGGLFEAQRVAAIADAAGIEL
HHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHCCCEEE
YGGTMLEGGIGTAASAQLCATLPTLTWGTELFGPLLLAEEILATPLAYADFALIVPSTPG
ECCEEEECCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHCCEEEEEECCCC
LGVELDMDRVDFFRRDRPAPRLSPVRQSVGA
CCEEECHHHHHHHHCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9473520; 9260969 [H]