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The map label for this gene is glcA [H]

Identifier: 154248270

GI number: 154248270

Start: 4819697

End: 4821382

Strand: Reverse

Name: glcA [H]

Synonym: Xaut_4350

Alternate gene names: 154248270

Gene position: 4821382-4819697 (Counterclockwise)

Preceding gene: 154248275

Following gene: 154248268

Centisome position: 90.82

GC content: 66.19

Gene sequence:

>1686_bases
ATGATCTGGTCTCAAGTCTATGACCCCATGGGCAGCATGTTGCTGTCCACCTTGCTCGCGGCCATACCGGTCGTGGTCCT
GCTCGGCGCCATCGGCATCTTCGAGATGCGCGCGCATCTGGCGGCGCTGCTTGGCCTCGCCAGTGCGCTCGTGGTGGCGG
TACTGGCCTTCGGCATGCCGGCGAAGATGGCGGGGATGTCCGCCCTTTACGGTGCCGCCTTCGGGCTCATGCCCATCGGC
TGGATCATCGTCAACGTCATCTTCCTGTACCAGCTCACCAGCGAGAAGGGGGAGTTCGACGTTCTCCAGCGTTCCATCCG
GGGCATCTCGGACGATCGGCGCCTGCAGCTCCTGTTCATCGCCTTCTCGTTCGGCGCCTTCTTCGAGGGCGCAGCCGGCT
TCGGCACCCCGGTGGCGGTCACCGCCGCCATGCTGATCGGCCTTGGCTTCTCGCCGCTCGCCGCGTCTGGCCTCTCGCTC
ATCGCCAATACCGCGCCGGTGGCTTATGGCGCCCTGGGCACGCCGGTGATCGCCCTCGCCGCCGTCACCGGGCTCGACCT
GCAGCAGCTCTCGGGCATGATCGGCCGGCAGTTGCCGTTCTTCTCCCTGATCGTGCCGTTCTGGCTGATCTGGGCCTTCG
TCGGCTTCCGCAAGATGCTTGAGGTGTGGCCGGCGCTGCTCGTGGCCGGCCTGTCGTTCGCGGTGCCGCAGTATCTCGTC
TCCAACTTCCACGGCCCGTGGCTGGTGGACGTGGTGGCCGCCATCTCCTCCATGGTCGCCCTGGCCCTGTTCCTGCGGGT
CTGGCAGCCCAAGCGCCCCATGACCGAAGCGCCCCGCGATTGGTCGTCCAAGGACATCGAGGACACCGGCCACATGCCCG
TCACCGAGGCTCAGAAGCCCGGCGCGGTGTTCAAGGCCTGGCTGCCCTGGATCATCCTTTCCGTGTTCGTGTTCATCTGG
GGCGTGCCCGAGGCCAAGGCCTTCTTCGACAGCATCTGGCTGGCCAAGTTCCCGGTGGAAGGCCTGCATCAGCTGGTGCA
GAAGGTTCCCCCGGTGGTGGCCGCGCCCCATACCGAGGCGGCGGTGTTCAATCTCAACCTGGTCTCAGCCACCGGCACCG
GCATCCTGCTCGCCGGCATCGTCTCCGGGCTCGTGCTCGGCTACAGCCCGGCGGGCCTCGTGAAGATGTACGGCAAGACG
CTGTACATGATCCGCTACTCGCTGCTCACCATCGCCTGCATGCTGGCGCTGGGCTTTGTCACCCGCTATTCGGGGCTCGA
CGCCACGCTGGGCCTTGCCTTCGCCCATACCGGCTGGTTCTACCCGCTGTTCGGCACCCTGCTCGGCTGGGTCGGCGTGG
CGGTGACCGGTTCGGACACGGCCTCCAACGTGCTGTTCGGCGGCCTGCAGAAGATCACCGCGCAGCAGCTTCACCTGAAC
CCGGTGCTGATGGCTGCCGCCAACTCCTCCGGCGGCGTGATGGGCAAGATGATCGACGCCCAGTCCATCGTCGTCGCCTC
CACTGCCACCAAGTGGTACGGGCATGAAGGCTCCATCCTGCGCTATGTGTTCTTCCACTCCATCGCGCTGGCGGTGCTGG
TCGGCTTGCTGATCATGGCCCAGGCCTATCTGCCGCCCTTCACAAGCATGGTTCCCAGCGTCATCGCGCCGGTGGTGGGC
CACTGA

Upstream 100 bases:

>100_bases
ACTCACATACCGATAACACCGCTCAGAGGCAGCAAAGAAGGCACGGACCGCATCGCGGGTCCGAACGCCCCCGCTTGTCC
TGAGGCTGGAGGGGCACCCG

Downstream 100 bases:

>100_bases
CCGACGCATATCCGCCGGCGCGGCCCTCGCGCCGGCGGATCGCCGTTCCCGGACGAAGGGAGGGGCTTCAAAGGCTTTTG
ATATCCACGCCGGTCCCCGC

Product: L-lactate transport

Products: lactate [Cytoplasm]; Proton [Cytoplasm]; glycolate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 561; Mature: 561

Protein sequence:

>561_residues
MIWSQVYDPMGSMLLSTLLAAIPVVVLLGAIGIFEMRAHLAALLGLASALVVAVLAFGMPAKMAGMSALYGAAFGLMPIG
WIIVNVIFLYQLTSEKGEFDVLQRSIRGISDDRRLQLLFIAFSFGAFFEGAAGFGTPVAVTAAMLIGLGFSPLAASGLSL
IANTAPVAYGALGTPVIALAAVTGLDLQQLSGMIGRQLPFFSLIVPFWLIWAFVGFRKMLEVWPALLVAGLSFAVPQYLV
SNFHGPWLVDVVAAISSMVALALFLRVWQPKRPMTEAPRDWSSKDIEDTGHMPVTEAQKPGAVFKAWLPWIILSVFVFIW
GVPEAKAFFDSIWLAKFPVEGLHQLVQKVPPVVAAPHTEAAVFNLNLVSATGTGILLAGIVSGLVLGYSPAGLVKMYGKT
LYMIRYSLLTIACMLALGFVTRYSGLDATLGLAFAHTGWFYPLFGTLLGWVGVAVTGSDTASNVLFGGLQKITAQQLHLN
PVLMAAANSSGGVMGKMIDAQSIVVASTATKWYGHEGSILRYVFFHSIALAVLVGLLIMAQAYLPPFTSMVPSVIAPVVG
H

Sequences:

>Translated_561_residues
MIWSQVYDPMGSMLLSTLLAAIPVVVLLGAIGIFEMRAHLAALLGLASALVVAVLAFGMPAKMAGMSALYGAAFGLMPIG
WIIVNVIFLYQLTSEKGEFDVLQRSIRGISDDRRLQLLFIAFSFGAFFEGAAGFGTPVAVTAAMLIGLGFSPLAASGLSL
IANTAPVAYGALGTPVIALAAVTGLDLQQLSGMIGRQLPFFSLIVPFWLIWAFVGFRKMLEVWPALLVAGLSFAVPQYLV
SNFHGPWLVDVVAAISSMVALALFLRVWQPKRPMTEAPRDWSSKDIEDTGHMPVTEAQKPGAVFKAWLPWIILSVFVFIW
GVPEAKAFFDSIWLAKFPVEGLHQLVQKVPPVVAAPHTEAAVFNLNLVSATGTGILLAGIVSGLVLGYSPAGLVKMYGKT
LYMIRYSLLTIACMLALGFVTRYSGLDATLGLAFAHTGWFYPLFGTLLGWVGVAVTGSDTASNVLFGGLQKITAQQLHLN
PVLMAAANSSGGVMGKMIDAQSIVVASTATKWYGHEGSILRYVFFHSIALAVLVGLLIMAQAYLPPFTSMVPSVIAPVVG
H
>Mature_561_residues
MIWSQVYDPMGSMLLSTLLAAIPVVVLLGAIGIFEMRAHLAALLGLASALVVAVLAFGMPAKMAGMSALYGAAFGLMPIG
WIIVNVIFLYQLTSEKGEFDVLQRSIRGISDDRRLQLLFIAFSFGAFFEGAAGFGTPVAVTAAMLIGLGFSPLAASGLSL
IANTAPVAYGALGTPVIALAAVTGLDLQQLSGMIGRQLPFFSLIVPFWLIWAFVGFRKMLEVWPALLVAGLSFAVPQYLV
SNFHGPWLVDVVAAISSMVALALFLRVWQPKRPMTEAPRDWSSKDIEDTGHMPVTEAQKPGAVFKAWLPWIILSVFVFIW
GVPEAKAFFDSIWLAKFPVEGLHQLVQKVPPVVAAPHTEAAVFNLNLVSATGTGILLAGIVSGLVLGYSPAGLVKMYGKT
LYMIRYSLLTIACMLALGFVTRYSGLDATLGLAFAHTGWFYPLFGTLLGWVGVAVTGSDTASNVLFGGLQKITAQQLHLN
PVLMAAANSSGGVMGKMIDAQSIVVASTATKWYGHEGSILRYVFFHSIALAVLVGLLIMAQAYLPPFTSMVPSVIAPVVG
H

Specific function: Transports glycolate across the membrane. Can also transport L-lactate and D-lactate. Seems to be driven by a proton motive force [H]

COG id: COG1620

COG function: function code C; L-lactate permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lactate permease family [H]

Homologues:

Organism=Escherichia coli, GI1789347, Length=559, Percent_Identity=45.7960644007156, Blast_Score=459, Evalue=1e-130,
Organism=Escherichia coli, GI1790031, Length=556, Percent_Identity=43.5251798561151, Blast_Score=429, Evalue=1e-121,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003804 [H]

Pfam domain/function: PF02652 Lactate_perm [H]

EC number: NA

Molecular weight: Translated: 59755; Mature: 59755

Theoretical pI: Translated: 9.07; Mature: 9.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure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HHHCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: lactate [Periplasm]; Proton [Periplasm]; glycolate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + lactate [Periplasm] = Proton [Cytoplasm] + lactate [Cytoplasm] Proton [Periplasm] + glycolate [Periplasm] = Proton [Cytoplasm] + glycolate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503; 11283302; 11785976 [H]