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The map label for this gene is metG [H]

Identifier: 154248247

GI number: 154248247

Start: 4799650

End: 4801302

Strand: Direct

Name: metG [H]

Synonym: Xaut_4327

Alternate gene names: 154248247

Gene position: 4799650-4801302 (Clockwise)

Preceding gene: 154248246

Following gene: 154248248

Centisome position: 90.41

GC content: 67.09

Gene sequence:

>1653_bases
GTGTCTTTGCCGATCTCGCCGAAGCCGTGCGCTGAGAAGCCGCCGCGACGCGCGGGGCCGGAGCCCTTTTCCGAGCAAGC
CTGTTTCGAGCGCCCGATGTCCGCGAAGCCCCCCTTCTACATCACCACCGCCATCGCCTATCCCAACGGCGTGCCCCACA
TGGGCCACGCCTATGAGGCGATCGCGACCGACTGCATCGCGCGCTTCAAGCGGCTTGACGGCTATGACGTGCGGTTCCTC
ACGGGCACGGACGAGCACGGCATCAAGATGCTCCAGACCGCCCAGAAGGCCGGCGTCACCCCGCGTGAGCTGCTGGCGCG
CAACGTGCCGCGCTTCGAGGCCATGGTGGAGGCCTTCAACCTCTCCATCGACGACTTCATCCGCACCACCCAGGAGCGGC
ACCACGCCTCCAGCCAGGCCATCTGGGCCAAGATGGCCGCCAACGGCGACATCTACAAATCCACCTATGCCGGCTGGTAT
TCGGTGCGCGACGAGGCCTATTACGCCGAGGACGAGACCCAGCTGAACGAGCAGGGCATCCGCCTCGGTCCCCAGGGCAC
GCCGGTGGAATGGGTGGAGGAGGAAAGCTACTTCTTCCGCCTCTCCGCCTATCAGGACCGGCTTCTGGCTTACTACGCGG
AAAACCCCGGCTTCATCATGCCGGAGGCCCGGCGCAACGAGGTGGTGAGCTTCGTCTCCGGCGGCCTCACCGACCTCTCC
ATCTCCCGCACCACCTTCGACTGGGGCATCCCGGTGCCCGGCGACGACAAGCACATCATGTATGTGTGGGTGGACGCGCT
GACCAACTACATCACCGCGCTCGGCTATCCGGACACGGAGGCGGAGCTGTTCAAGCGGTTCTGGCCGGCGAACCTGCACG
TGATCGGCAAGGACATCATCCGCTTCCACGCGGTCTACTGGCCGGCCTTCCTGTGGTCGGCGGGGCTGGAGGCGCCGAAG
CGCGTGTTCGGCCACGGCTTCCTGTTCAACCGCGGGGAGAAGATGTCCAAGTCGGTGGGCAATGTGATCGACCCGTTCGA
TCTCGCCGCCACCTACGGCGTGGACGCCATCCGCTACTTCTTCCTGCGCGAGGTGTCGTTCGGGCAGGACGGCTCCTACA
GCCACGAGGCCATTGTGGCGCGCATGAATGCGGACCTTGCCAACGATCTCGGCAACCTGGCCCAGCGCTCCCTGTCCATG
ATCGCCAAGAATCTCGATGGCGTGGTGCCGCAGCCCGGCCCGCTGTCGCCGGAGGACGAGGCCATCCTCGCCCTCGCCGA
CGGCATGCTGGAGAAGAGCCGTGAGGCCATGGAGGGCCAGGCCATCCACCACTATCTCGCCGCGGTATGGAATGTGGTGG
CCGAGGCCAATCGCTATTTCGCCGCCCAGGCTCCGTGGGCCCTGCGCAAGACCGATCCCGCCCGCATGGCCACCGTGCTG
TGGGTGACCGCCGAGCTGATCCGCGAGGTGGGCATCCTGGTGCAGCCGGTGATGCCCGAAAGCGCTGCCAGGCTGCTCGA
CCTCGTGGTGGTGCCGGCGGACGAGCGCGGCTTCGACCGGCTGGGCGCCGGCCACCGGCTGCCGGCCGGCCGCGTTCTGC
CCGCGCCAGTGGGCGTGTTCCCGCGCTATGTGGAGCCGGAAACGGCCTCGTGA

Upstream 100 bases:

>100_bases
CGCCATCGCCTTGTCCGCCTCGGCGAGGTATGGGAGAAGGTGCGGCGCAGCGCGCGCGAGGCGGATGTCTTCAATCTCGA
TCGCAAGGCGCTGGTGTTCC

Downstream 100 bases:

>100_bases
GCGCAAAACCGCGCGACGGGATGGATGGCGGACCCTAAAGTGTCCTATACGGGGCTCTGGTCATGCCGATGCGATCGGGA
TCCATTTCGCTCATGCGCTC

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 550; Mature: 549

Protein sequence:

>550_residues
MSLPISPKPCAEKPPRRAGPEPFSEQACFERPMSAKPPFYITTAIAYPNGVPHMGHAYEAIATDCIARFKRLDGYDVRFL
TGTDEHGIKMLQTAQKAGVTPRELLARNVPRFEAMVEAFNLSIDDFIRTTQERHHASSQAIWAKMAANGDIYKSTYAGWY
SVRDEAYYAEDETQLNEQGIRLGPQGTPVEWVEEESYFFRLSAYQDRLLAYYAENPGFIMPEARRNEVVSFVSGGLTDLS
ISRTTFDWGIPVPGDDKHIMYVWVDALTNYITALGYPDTEAELFKRFWPANLHVIGKDIIRFHAVYWPAFLWSAGLEAPK
RVFGHGFLFNRGEKMSKSVGNVIDPFDLAATYGVDAIRYFFLREVSFGQDGSYSHEAIVARMNADLANDLGNLAQRSLSM
IAKNLDGVVPQPGPLSPEDEAILALADGMLEKSREAMEGQAIHHYLAAVWNVVAEANRYFAAQAPWALRKTDPARMATVL
WVTAELIREVGILVQPVMPESAARLLDLVVVPADERGFDRLGAGHRLPAGRVLPAPVGVFPRYVEPETAS

Sequences:

>Translated_550_residues
MSLPISPKPCAEKPPRRAGPEPFSEQACFERPMSAKPPFYITTAIAYPNGVPHMGHAYEAIATDCIARFKRLDGYDVRFL
TGTDEHGIKMLQTAQKAGVTPRELLARNVPRFEAMVEAFNLSIDDFIRTTQERHHASSQAIWAKMAANGDIYKSTYAGWY
SVRDEAYYAEDETQLNEQGIRLGPQGTPVEWVEEESYFFRLSAYQDRLLAYYAENPGFIMPEARRNEVVSFVSGGLTDLS
ISRTTFDWGIPVPGDDKHIMYVWVDALTNYITALGYPDTEAELFKRFWPANLHVIGKDIIRFHAVYWPAFLWSAGLEAPK
RVFGHGFLFNRGEKMSKSVGNVIDPFDLAATYGVDAIRYFFLREVSFGQDGSYSHEAIVARMNADLANDLGNLAQRSLSM
IAKNLDGVVPQPGPLSPEDEAILALADGMLEKSREAMEGQAIHHYLAAVWNVVAEANRYFAAQAPWALRKTDPARMATVL
WVTAELIREVGILVQPVMPESAARLLDLVVVPADERGFDRLGAGHRLPAGRVLPAPVGVFPRYVEPETAS
>Mature_549_residues
SLPISPKPCAEKPPRRAGPEPFSEQACFERPMSAKPPFYITTAIAYPNGVPHMGHAYEAIATDCIARFKRLDGYDVRFLT
GTDEHGIKMLQTAQKAGVTPRELLARNVPRFEAMVEAFNLSIDDFIRTTQERHHASSQAIWAKMAANGDIYKSTYAGWYS
VRDEAYYAEDETQLNEQGIRLGPQGTPVEWVEEESYFFRLSAYQDRLLAYYAENPGFIMPEARRNEVVSFVSGGLTDLSI
SRTTFDWGIPVPGDDKHIMYVWVDALTNYITALGYPDTEAELFKRFWPANLHVIGKDIIRFHAVYWPAFLWSAGLEAPKR
VFGHGFLFNRGEKMSKSVGNVIDPFDLAATYGVDAIRYFFLREVSFGQDGSYSHEAIVARMNADLANDLGNLAQRSLSMI
AKNLDGVVPQPGPLSPEDEAILALADGMLEKSREAMEGQAIHHYLAAVWNVVAEANRYFAAQAPWALRKTDPARMATVLW
VTAELIREVGILVQPVMPESAARLLDLVVVPADERGFDRLGAGHRLPAGRVLPAPVGVFPRYVEPETAS

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2B subfamily [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=548, Percent_Identity=38.5036496350365, Blast_Score=352, Evalue=4e-97,
Organism=Homo sapiens, GI14043022, Length=528, Percent_Identity=24.8106060606061, Blast_Score=142, Evalue=6e-34,
Organism=Escherichia coli, GI1788432, Length=501, Percent_Identity=26.9461077844311, Blast_Score=193, Evalue=3e-50,
Organism=Caenorhabditis elegans, GI212645096, Length=527, Percent_Identity=36.2428842504744, Blast_Score=323, Evalue=2e-88,
Organism=Caenorhabditis elegans, GI71992781, Length=417, Percent_Identity=35.7314148681055, Blast_Score=248, Evalue=8e-66,
Organism=Caenorhabditis elegans, GI17540794, Length=528, Percent_Identity=26.3257575757576, Blast_Score=154, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6321610, Length=534, Percent_Identity=32.7715355805243, Blast_Score=277, Evalue=3e-75,
Organism=Saccharomyces cerevisiae, GI6321704, Length=540, Percent_Identity=24.8148148148148, Blast_Score=124, Evalue=4e-29,
Organism=Drosophila melanogaster, GI21358483, Length=503, Percent_Identity=37.7733598409543, Blast_Score=337, Evalue=1e-92,
Organism=Drosophila melanogaster, GI24655311, Length=563, Percent_Identity=25.3996447602131, Blast_Score=143, Evalue=3e-34,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 61343; Mature: 61212

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLPISPKPCAEKPPRRAGPEPFSEQACFERPMSAKPPFYITTAIAYPNGVPHMGHAYEA
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCEEEEEEEECCCCCCCCCHHHHH
IATDCIARFKRLDGYDVRFLTGTDEHGIKMLQTAQKAGVTPRELLARNVPRFEAMVEAFN
HHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHC
LSIDDFIRTTQERHHASSQAIWAKMAANGDIYKSTYAGWYSVRDEAYYAEDETQLNEQGI
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHCCCCEEECCCEEECCCHHHHCCCCC
RLGPQGTPVEWVEEESYFFRLSAYQDRLLAYYAENPGFIMPEARRNEVVSFVSGGLTDLS
EECCCCCCHHHEECCCEEEEEHHHHCCEEEEEECCCCEECCCHHHHHHHHHHHCCCCEEE
ISRTTFDWGIPVPGDDKHIMYVWVDALTNYITALGYPDTEAELFKRFWPANLHVIGKDII
EEEEEEECCCCCCCCCCEEEEEEHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEHHHHHH
RFHAVYWPAFLWSAGLEAPKRVFGHGFLFNRGEKMSKSVGNVIDPFDLAATYGVDAIRYF
HHHHHHHHHHHHHHCCCHHHHHHHCCCEECCCHHHHHHHCCCCCCHHHHHHHHHHHHHHH
FLREVSFGQDGSYSHEAIVARMNADLANDLGNLAQRSLSMIAKNLDGVVPQPGPLSPEDE
HHHHHCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
AILALADGMLEKSREAMEGQAIHHYLAAVWNVVAEANRYFAAQAPWALRKTDPARMATVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCHHHHHHHH
WVTAELIREVGILVQPVMPESAARLLDLVVVPADERGFDRLGAGHRLPAGRVLPAPVGVF
HHHHHHHHHHHHHCCCCCCHHHHHHHHEEEECCCCCCHHHHCCCCCCCCCCCCCCCCCCC
PRYVEPETAS
CCCCCCCCCC
>Mature Secondary Structure 
SLPISPKPCAEKPPRRAGPEPFSEQACFERPMSAKPPFYITTAIAYPNGVPHMGHAYEA
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCEEEEEEEECCCCCCCCCHHHHH
IATDCIARFKRLDGYDVRFLTGTDEHGIKMLQTAQKAGVTPRELLARNVPRFEAMVEAFN
HHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHC
LSIDDFIRTTQERHHASSQAIWAKMAANGDIYKSTYAGWYSVRDEAYYAEDETQLNEQGI
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHCCCCEEECCCEEECCCHHHHCCCCC
RLGPQGTPVEWVEEESYFFRLSAYQDRLLAYYAENPGFIMPEARRNEVVSFVSGGLTDLS
EECCCCCCHHHEECCCEEEEEHHHHCCEEEEEECCCCEECCCHHHHHHHHHHHCCCCEEE
ISRTTFDWGIPVPGDDKHIMYVWVDALTNYITALGYPDTEAELFKRFWPANLHVIGKDII
EEEEEEECCCCCCCCCCEEEEEEHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEHHHHHH
RFHAVYWPAFLWSAGLEAPKRVFGHGFLFNRGEKMSKSVGNVIDPFDLAATYGVDAIRYF
HHHHHHHHHHHHHHCCCHHHHHHHCCCEECCCHHHHHHHCCCCCCHHHHHHHHHHHHHHH
FLREVSFGQDGSYSHEAIVARMNADLANDLGNLAQRSLSMIAKNLDGVVPQPGPLSPEDE
HHHHHCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
AILALADGMLEKSREAMEGQAIHHYLAAVWNVVAEANRYFAAQAPWALRKTDPARMATVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCHHHHHHHH
WVTAELIREVGILVQPVMPESAARLLDLVVVPADERGFDRLGAGHRLPAGRVLPAPVGVF
HHHHHHHHHHHHHCCCCCCHHHHHHHHEEEECCCCCCHHHHCCCCCCCCCCCCCCCCCCC
PRYVEPETAS
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]