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The map label for this gene is holB [H]

Identifier: 154248246

GI number: 154248246

Start: 4798641

End: 4799684

Strand: Direct

Name: holB [H]

Synonym: Xaut_4326

Alternate gene names: 154248246

Gene position: 4798641-4799684 (Clockwise)

Preceding gene: 154248245

Following gene: 154248247

Centisome position: 90.39

GC content: 71.65

Gene sequence:

>1044_bases
ATGGCGCAGGCCGTGAGCGAGATCGTCTCCGGCGATGTCCTGCCCGGCGCGCCACACCCGCGCGACCAGGCGCGGCTGTT
CGGCCACGGGGTGGTGGAAGCCGACCTGATCGGCGATTTCCGCGCCGGCCGGTTGCCCCATGCCCTGCTCATCGGCGGGC
CGGAAGGAATCGGCAAGGCGACGCTGGCCTATCGTCTCGCCCGCATGGTGCTGTCCGGCGGACCTGAGGCCGGCAGCCAT
TCGCTGGATCTGCCCGTCACCCATCCCGTGTTCCGGCAGGTGGCGTCCCAGAGCCATCCGGACCTTTTGGTGCTGCACCG
GGTGCCGGAGGCGGGGGAGGAGAAGATCCCCACCGTCATTCCGGCCGAGATGGTGCGCCGCGTGCGTAGCTTCTTCGGCG
CCACGGCGGCGGCCGGGGGCTGGCGCGTCTGCATCGTGGACGCGGTGGACGAGCTCAATCCGTTCGGCGCCAACGCGCTG
CTGAAGACGCTGGAGGAGCCGCCGCCCAGGGCGCTGTTCCTGCTCATCTCCCACGCTCCGGGCCGGGTGCTGCCCACCAT
CCGCTCCCGCACCCGCCTGCTGCGCATGCGCCCGCTGGGCAGCGCCGACGTGCTCGCTGCCCTCGATCACCTCAAGGAGG
AGATGGCGGATCTCGACGCCGCCACCCTGCCATCCGCCGCCGAGGCCAGCGGCGGCAGCGTGCGCCGGGCCATCGTGCTG
GCGCGCGGGGAGGGGATGGAGGTGCGCTCCGCCACCCTGAAGGCGCTGGAGCAGTTGCCCCGGCCCCGTCCGGAAGACCT
TCACGCGCTGGGTGCCCGCCTCCAGGGTGACCGCTCCGACGGGCTCGACCTCTTCATCGAGGCGGTGAGCGACTATGTGG
CCGCCCGCGCGACGCAGGGTGACGAGCCGCGCCATCGCCTTGTCCGCCTCGGCGAGGTATGGGAGAAGGTGCGGCGCAGC
GCGCGCGAGGCGGATGTCTTCAATCTCGATCGCAAGGCGCTGGTGTTCCGTGTCTTTGCCGATCTCGCCGAAGCCGTGCG
CTGA

Upstream 100 bases:

>100_bases
TGCTGGTGGACGCGACGCCCGATCCGGACGCGGTCGCGGCGCAGATCGCCGGCATCGTCGCCGCCCGCCTCGGACTTCAC
GCCACGGCCGAGGCGTCCTG

Downstream 100 bases:

>100_bases
GAAGCCGCCGCGACGCGCGGGGCCGGAGCCCTTTTCCGAGCAAGCCTGTTTCGAGCGCCCGATGTCCGCGAAGCCCCCCT
TCTACATCACCACCGCCATC

Product: DNA-directed DNA polymerase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 347; Mature: 346

Protein sequence:

>347_residues
MAQAVSEIVSGDVLPGAPHPRDQARLFGHGVVEADLIGDFRAGRLPHALLIGGPEGIGKATLAYRLARMVLSGGPEAGSH
SLDLPVTHPVFRQVASQSHPDLLVLHRVPEAGEEKIPTVIPAEMVRRVRSFFGATAAAGGWRVCIVDAVDELNPFGANAL
LKTLEEPPPRALFLLISHAPGRVLPTIRSRTRLLRMRPLGSADVLAALDHLKEEMADLDAATLPSAAEASGGSVRRAIVL
ARGEGMEVRSATLKALEQLPRPRPEDLHALGARLQGDRSDGLDLFIEAVSDYVAARATQGDEPRHRLVRLGEVWEKVRRS
AREADVFNLDRKALVFRVFADLAEAVR

Sequences:

>Translated_347_residues
MAQAVSEIVSGDVLPGAPHPRDQARLFGHGVVEADLIGDFRAGRLPHALLIGGPEGIGKATLAYRLARMVLSGGPEAGSH
SLDLPVTHPVFRQVASQSHPDLLVLHRVPEAGEEKIPTVIPAEMVRRVRSFFGATAAAGGWRVCIVDAVDELNPFGANAL
LKTLEEPPPRALFLLISHAPGRVLPTIRSRTRLLRMRPLGSADVLAALDHLKEEMADLDAATLPSAAEASGGSVRRAIVL
ARGEGMEVRSATLKALEQLPRPRPEDLHALGARLQGDRSDGLDLFIEAVSDYVAARATQGDEPRHRLVRLGEVWEKVRRS
AREADVFNLDRKALVFRVFADLAEAVR
>Mature_346_residues
AQAVSEIVSGDVLPGAPHPRDQARLFGHGVVEADLIGDFRAGRLPHALLIGGPEGIGKATLAYRLARMVLSGGPEAGSHS
LDLPVTHPVFRQVASQSHPDLLVLHRVPEAGEEKIPTVIPAEMVRRVRSFFGATAAAGGWRVCIVDAVDELNPFGANALL
KTLEEPPPRALFLLISHAPGRVLPTIRSRTRLLRMRPLGSADVLAALDHLKEEMADLDAATLPSAAEASGGSVRRAIVLA
RGEGMEVRSATLKALEQLPRPRPEDLHALGARLQGDRSDGLDLFIEAVSDYVAARATQGDEPRHRLVRLGEVWEKVRRSA
READVFNLDRKALVFRVFADLAEAVR

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787341, Length=222, Percent_Identity=33.7837837837838, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1786676, Length=243, Percent_Identity=29.6296296296296, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003959
- InterPro:   IPR004622 [H]

Pfam domain/function: PF00004 AAA [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 37439; Mature: 37307

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQAVSEIVSGDVLPGAPHPRDQARLFGHGVVEADLIGDFRAGRLPHALLIGGPEGIGKA
CHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCEEHHHHCCHHCCCCCCEEEEECCCCCHHH
TLAYRLARMVLSGGPEAGSHSLDLPVTHPVFRQVASQSHPDLLVLHRVPEAGEEKIPTVI
HHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCC
PAEMVRRVRSFFGATAAAGGWRVCIVDAVDELNPFGANALLKTLEEPPPRALFLLISHAP
CHHHHHHHHHHHCCCCCCCCCEEEEEECHHHCCCCCHHHHHHHHCCCCCCEEEEEEECCC
GRVLPTIRSRTRLLRMRPLGSADVLAALDHLKEEMADLDAATLPSAAEASGGSVRRAIVL
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCEEEEEEE
ARGEGMEVRSATLKALEQLPRPRPEDLHALGARLQGDRSDGLDLFIEAVSDYVAARATQG
ECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCC
DEPRHRLVRLGEVWEKVRRSAREADVFNLDRKALVFRVFADLAEAVR
CCHHHHHHHHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AQAVSEIVSGDVLPGAPHPRDQARLFGHGVVEADLIGDFRAGRLPHALLIGGPEGIGKA
HHHHHHHHCCCCCCCCCCCCHHHHHHHCCCEEHHHHCCHHCCCCCCEEEEECCCCCHHH
TLAYRLARMVLSGGPEAGSHSLDLPVTHPVFRQVASQSHPDLLVLHRVPEAGEEKIPTVI
HHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCC
PAEMVRRVRSFFGATAAAGGWRVCIVDAVDELNPFGANALLKTLEEPPPRALFLLISHAP
CHHHHHHHHHHHCCCCCCCCCEEEEEECHHHCCCCCHHHHHHHHCCCCCCEEEEEEECCC
GRVLPTIRSRTRLLRMRPLGSADVLAALDHLKEEMADLDAATLPSAAEASGGSVRRAIVL
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCEEEEEEE
ARGEGMEVRSATLKALEQLPRPRPEDLHALGARLQGDRSDGLDLFIEAVSDYVAARATQG
ECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCC
DEPRHRLVRLGEVWEKVRRSAREADVFNLDRKALVFRVFADLAEAVR
CCHHHHHHHHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550441; 10984043 [H]