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The map label for this gene is pcpB [H]

Identifier: 154248240

GI number: 154248240

Start: 4792425

End: 4793993

Strand: Direct

Name: pcpB [H]

Synonym: Xaut_4320

Alternate gene names: 154248240

Gene position: 4792425-4793993 (Clockwise)

Preceding gene: 154248238

Following gene: 154248241

Centisome position: 90.27

GC content: 70.36

Gene sequence:

>1569_bases
GTGGATGCTGCTGTAACGGTCCTCGTGGTGGGCGCCGGCCCAACCGGGCTTCTGCTGGCTGCGGAACTCCAGCGGCGCGG
CGTGGATTGCCTCCTCATCGACGCCCATGAGAGCCCGCTGGACTGGGACCGCGCCACCGTCGTGCACCCCCGTTCGCTTG
AGATCTTCGATGCCCTCGGCATTGTCGAGCCGCTGCTGGCGGCCGGGGTCAAGCAGCGGCTGGCGCGCATTCATTCCGAT
GGCAGCGTGCTGGGGGATATCGACCTCGCGCTCTGCGGCAGCCGCTATGCCTTCAATCTCGGCGTATCGGAGGAGGTGAC
GGAGGCGGTCCTGACCGACTACCTGACCGGTTTGGGCGGCACGGTCACCCGCGCGACCCGCCTCGTCAGCCTGGAGGAGC
GCCCCGACGGCGTGCTGGCGACCATGGAGAGCGAGGGCGCAACCACACAGGTCCTCGCCCAATGGGTGGTGGGATGCGAT
GGCAACCGCAGCACGGTGCGGACGCTGGCGGGCATCGCGCGGGACGGGCACGACATCGCCGAGCCCTGGGCGGTGTTCGA
CACCACCGTGGGCGGCTGGACCCAATCCTATGAGGCCAATTACGCCTATCTCGACGCCATTCCGGTGATTCTGACCGCGC
TGCCGGACCAGCGCTGGCGGGTCTATCTGCGGCCGAGTTCCGATGCCTCCGACCTGACCGCCGATGCCGCGATCACCCTC
GGGCGCTACGTGCCCGGCCTTCGGTTCGAGGACGTGGCCAACCCGACGCGCTTCCATTGCCACACCACCGTGGCGCGCAG
CTTCCGGGTCGGGCGCCTGCTGCTGGCCGGGGATGCCGCCCACACCTGCAGCCCGGCCCAGGGCCACGGCATGAATACCG
GACTGCAGGATGCCTTCAACCTGTCGTGGAAGCTGGCGCTGGTGTGCCAGGGCGTCTGCCCCGACACCCTGCTCGATAGC
TATGACGCGGAACGCCGCCCGGTGGCCCAATTGGTGACGGCCTCGGGCGATGCTGCCGAGAGCCTCCAGAGCGTGAAGGA
TCCCGACGAGCGCCGCGCCCGCGATGCCGCCATCCGCGCCGTGTTCGCCGACCCCACCTCACGCCATCACGAGGCGGTCG
CCGAGGCGGAGCTGGACATCGACTATGGCGGCTCGCCCATCGTGATGGGTGACCGGCACACCGCGCTCGCGCCGGGCCAG
CGGCTTCCGGACCGGATCGCGGCCCGGGCCGCCGATGGCGGAGCCTGCATGCTCAATGATTTCGCCAATCGGCCGGGCCA
CACCGCCATCCTGATTGGTAGCCCTTCGACGCCGCAGGAGGAACTGGCGCAGGTCCACGACGCCATCCGCTCCCGCCCCG
GCCTTGCCGTGCTCGAAGCCAGCTTCGCGCTGACCGCCCGCCCGGACGGTGCCGACGGACACGGGCGCGTCGAGCCCGCC
GTGGCGGACCGGCTGGGGGTCGGCGCCATCACGCTCCTGGTCGTCCGGCCCGACGGCCATGTGGGCCTCCGGGCCGATGC
GAACCATGCGGATGCCCTCGAGACTTATGGCGCGCTGCTGGGCGTCTGA

Upstream 100 bases:

>100_bases
TCCACCGTAGCCGCCGGCCGGGCCTCCAGCCCACGGCCAAGCCCTTGCTCTTATGGCGCCGCCGGTCATGATGGCCAGGC
TTTTTCGGGGAGAATGCGCC

Downstream 100 bases:

>100_bases
GATCTGATGTCCGAAGGAGCGGCAATCGCGGCGGAGGGGCGGGGGGCACATGGGCACGTTCGAGGTTCTCGATCCCCGCT
TCGGCAGGCTGGTCATCGCC

Product: monooxygenase FAD-binding

Products: NA

Alternate protein names: Pentachlorophenol hydroxylase [H]

Number of amino acids: Translated: 522; Mature: 522

Protein sequence:

>522_residues
MDAAVTVLVVGAGPTGLLLAAELQRRGVDCLLIDAHESPLDWDRATVVHPRSLEIFDALGIVEPLLAAGVKQRLARIHSD
GSVLGDIDLALCGSRYAFNLGVSEEVTEAVLTDYLTGLGGTVTRATRLVSLEERPDGVLATMESEGATTQVLAQWVVGCD
GNRSTVRTLAGIARDGHDIAEPWAVFDTTVGGWTQSYEANYAYLDAIPVILTALPDQRWRVYLRPSSDASDLTADAAITL
GRYVPGLRFEDVANPTRFHCHTTVARSFRVGRLLLAGDAAHTCSPAQGHGMNTGLQDAFNLSWKLALVCQGVCPDTLLDS
YDAERRPVAQLVTASGDAAESLQSVKDPDERRARDAAIRAVFADPTSRHHEAVAEAELDIDYGGSPIVMGDRHTALAPGQ
RLPDRIAARAADGGACMLNDFANRPGHTAILIGSPSTPQEELAQVHDAIRSRPGLAVLEASFALTARPDGADGHGRVEPA
VADRLGVGAITLLVVRPDGHVGLRADANHADALETYGALLGV

Sequences:

>Translated_522_residues
MDAAVTVLVVGAGPTGLLLAAELQRRGVDCLLIDAHESPLDWDRATVVHPRSLEIFDALGIVEPLLAAGVKQRLARIHSD
GSVLGDIDLALCGSRYAFNLGVSEEVTEAVLTDYLTGLGGTVTRATRLVSLEERPDGVLATMESEGATTQVLAQWVVGCD
GNRSTVRTLAGIARDGHDIAEPWAVFDTTVGGWTQSYEANYAYLDAIPVILTALPDQRWRVYLRPSSDASDLTADAAITL
GRYVPGLRFEDVANPTRFHCHTTVARSFRVGRLLLAGDAAHTCSPAQGHGMNTGLQDAFNLSWKLALVCQGVCPDTLLDS
YDAERRPVAQLVTASGDAAESLQSVKDPDERRARDAAIRAVFADPTSRHHEAVAEAELDIDYGGSPIVMGDRHTALAPGQ
RLPDRIAARAADGGACMLNDFANRPGHTAILIGSPSTPQEELAQVHDAIRSRPGLAVLEASFALTARPDGADGHGRVEPA
VADRLGVGAITLLVVRPDGHVGLRADANHADALETYGALLGV
>Mature_522_residues
MDAAVTVLVVGAGPTGLLLAAELQRRGVDCLLIDAHESPLDWDRATVVHPRSLEIFDALGIVEPLLAAGVKQRLARIHSD
GSVLGDIDLALCGSRYAFNLGVSEEVTEAVLTDYLTGLGGTVTRATRLVSLEERPDGVLATMESEGATTQVLAQWVVGCD
GNRSTVRTLAGIARDGHDIAEPWAVFDTTVGGWTQSYEANYAYLDAIPVILTALPDQRWRVYLRPSSDASDLTADAAITL
GRYVPGLRFEDVANPTRFHCHTTVARSFRVGRLLLAGDAAHTCSPAQGHGMNTGLQDAFNLSWKLALVCQGVCPDTLLDS
YDAERRPVAQLVTASGDAAESLQSVKDPDERRARDAAIRAVFADPTSRHHEAVAEAELDIDYGGSPIVMGDRHTALAPGQ
RLPDRIAARAADGGACMLNDFANRPGHTAILIGSPSTPQEELAQVHDAIRSRPGLAVLEASFALTARPDGADGHGRVEPA
VADRLGVGAITLLVVRPDGHVGLRADANHADALETYGALLGV

Specific function: Dechlorination of pentachlorophenol to tetrachlorohydroquinone. Removes also hydrogen and nitro, amino, and cyano groups from benzene ring at the para position in relation to the hydroxyl of phenol [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=334, Percent_Identity=29.0419161676647, Blast_Score=131, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: =1.14.13.50 [H]

Molecular weight: Translated: 55286; Mature: 55286

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAAVTVLVVGAGPTGLLLAAELQRRGVDCLLIDAHESPLDWDRATVVHPRSLEIFDALG
CCCEEEEEEECCCCCHHHEEHHHHHCCCCEEEEECCCCCCCCCCEEEECCCCHHHHHHHH
IVEPLLAAGVKQRLARIHSDGSVLGDIDLALCGSRYAFNLGVSEEVTEAVLTDYLTGLGG
HHHHHHHHHHHHHHHHHCCCCCEEECCHHEEECCCEEEECCCHHHHHHHHHHHHHHCCCC
TVTRATRLVSLEERPDGVLATMESEGATTQVLAQWVVGCDGNRSTVRTLAGIARDGHDIA
HHHHHHHEEEECCCCCCEEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHH
EPWAVFDTTVGGWTQSYEANYAYLDAIPVILTALPDQRWRVYLRPSSDASDLTADAAITL
CCHHHEECCCCCCCCCCCCCEEEHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHH
GRYVPGLRFEDVANPTRFHCHTTVARSFRVGRLLLAGDAAHTCSPAQGHGMNTGLQDAFN
HHHCCCCCHHHCCCCCEEEEHHHHHHHHHHCEEEEECCCCCCCCCCCCCCCCCCCHHHHC
LSWKLALVCQGVCPDTLLDSYDAERRPVAQLVTASGDAAESLQSVKDPDERRARDAAIRA
CCEEEEEEECCCCCHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHEEE
VFADPTSRHHEAVAEAELDIDYGGSPIVMGDRHTALAPGQRLPDRIAARAADGGACMLND
EECCCCCHHHHHHHHHEEEECCCCCEEEECCCCCCCCCCCCCCHHHHHHCCCCCEEEEHH
FANRPGHTAILIGSPSTPQEELAQVHDAIRSRPGLAVLEASFALTARPDGADGHGRVEPA
HHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCCCCCCCCHH
VADRLGVGAITLLVVRPDGHVGLRADANHADALETYGALLGV
HHHHCCCCEEEEEEECCCCCEEEEECCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MDAAVTVLVVGAGPTGLLLAAELQRRGVDCLLIDAHESPLDWDRATVVHPRSLEIFDALG
CCCEEEEEEECCCCCHHHEEHHHHHCCCCEEEEECCCCCCCCCCEEEECCCCHHHHHHHH
IVEPLLAAGVKQRLARIHSDGSVLGDIDLALCGSRYAFNLGVSEEVTEAVLTDYLTGLGG
HHHHHHHHHHHHHHHHHCCCCCEEECCHHEEECCCEEEECCCHHHHHHHHHHHHHHCCCC
TVTRATRLVSLEERPDGVLATMESEGATTQVLAQWVVGCDGNRSTVRTLAGIARDGHDIA
HHHHHHHEEEECCCCCCEEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHH
EPWAVFDTTVGGWTQSYEANYAYLDAIPVILTALPDQRWRVYLRPSSDASDLTADAAITL
CCHHHEECCCCCCCCCCCCCEEEHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHH
GRYVPGLRFEDVANPTRFHCHTTVARSFRVGRLLLAGDAAHTCSPAQGHGMNTGLQDAFN
HHHCCCCCHHHCCCCCEEEEHHHHHHHHHHCEEEEECCCCCCCCCCCCCCCCCCCHHHHC
LSWKLALVCQGVCPDTLLDSYDAERRPVAQLVTASGDAAESLQSVKDPDERRARDAAIRA
CCEEEEEEECCCCCHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHEEE
VFADPTSRHHEAVAEAELDIDYGGSPIVMGDRHTALAPGQRLPDRIAARAADGGACMLND
EECCCCCHHHHHHHHHEEEECCCCCEEEECCCCCCCCCCCCCCHHHHHHCCCCCEEEEHH
FANRPGHTAILIGSPSTPQEELAQVHDAIRSRPGLAVLEASFALTARPDGADGHGRVEPA
HHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCCCCCCCCHH
VADRLGVGAITLLVVRPDGHVGLRADANHADALETYGALLGV
HHHHCCCCEEEEEEECCCCCEEEEECCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7678243; 12169590 [H]