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The map label for this gene is rhtB [H]

Identifier: 154248238

GI number: 154248238

Start: 4790539

End: 4791150

Strand: Direct

Name: rhtB [H]

Synonym: Xaut_4318

Alternate gene names: 154248238

Gene position: 4790539-4791150 (Clockwise)

Preceding gene: 154248237

Following gene: 154248240

Centisome position: 90.24

GC content: 70.75

Gene sequence:

>612_bases
ATGATATCTGCGGAAAGCCTTGCCCTGTTCGTGGTTGCCTGCGTCGCTCTTGCCGCGACGCCGGGGCCGAACGTAGCGCT
CATCGTCGGCACCAGCCTCAAGCACGGGCGGCGAGCCGGCATCCTCACCGCCACCGGCGTCAATGTGGGGCTCATCGCCC
AACTCGCGCTCGTGGCGGCGGGCCTCGCCTATGTGGTGGAAACCTTCTCCCGCAATTTCGACCTCGTGCGCTATGCGGGT
GCGGCCTATCTGGTCTGGCTCGCCGTGCTGCAATGGCGCGGGGCGGGAGCCGGTCGCGAGGCTCCCATGCCATCGGCCCG
CGCCGCCTTCGGGCGGGGGCTGGCGGTGGCCTTCGCCAATCCCAAGACCCTGCTGTTCCACGCGGCCTTCCTGCCCCAGT
TCATCACCGACAGGGCCGATCCCGTGCCGCAGATCGCGCTGCTGGCGGCGGTGTTCGCCGCGATCGCGCTGGTGGGCGAC
GTGCTGTGGGCCGTGGTGGCGGACAAGGCCCGCGCGGCGCTCGCCGGCCGCTTCACCCGCATCGCGGATCGGGTCTCAGC
CGGCATTCTCGTGGGCGGGGCCGCATTGCTGCTGGCGGCGGGACGGCGTTAA

Upstream 100 bases:

>100_bases
GACCGCCGCTCCGGCATCGTGCGATGCTGGAGCGTAGCTGAGCCCATCGTTGGTCAGCGCGCCAATGGCGATCGGGCTGC
CGCCGGAACGGGGGATGGCG

Downstream 100 bases:

>100_bases
AGCGCCGACCCCTTGCCTCACCGCGTATGCTGCTCGGCGAAGGCGGCGGCCGCGCCATAGAGGCCGGGCTGGGGATGGGT
AATGAGGCGGACCGGGATGT

Product: lysine exporter protein LysE/YggA

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 203; Mature: 203

Protein sequence:

>203_residues
MISAESLALFVVACVALAATPGPNVALIVGTSLKHGRRAGILTATGVNVGLIAQLALVAAGLAYVVETFSRNFDLVRYAG
AAYLVWLAVLQWRGAGAGREAPMPSARAAFGRGLAVAFANPKTLLFHAAFLPQFITDRADPVPQIALLAAVFAAIALVGD
VLWAVVADKARAALAGRFTRIADRVSAGILVGGAALLLAAGRR

Sequences:

>Translated_203_residues
MISAESLALFVVACVALAATPGPNVALIVGTSLKHGRRAGILTATGVNVGLIAQLALVAAGLAYVVETFSRNFDLVRYAG
AAYLVWLAVLQWRGAGAGREAPMPSARAAFGRGLAVAFANPKTLLFHAAFLPQFITDRADPVPQIALLAAVFAAIALVGD
VLWAVVADKARAALAGRFTRIADRVSAGILVGGAALLLAAGRR
>Mature_203_residues
MISAESLALFVVACVALAATPGPNVALIVGTSLKHGRRAGILTATGVNVGLIAQLALVAAGLAYVVETFSRNFDLVRYAG
AAYLVWLAVLQWRGAGAGREAPMPSARAAFGRGLAVAFANPKTLLFHAAFLPQFITDRADPVPQIALLAAVFAAIALVGD
VLWAVVADKARAALAGRFTRIADRVSAGILVGGAALLLAAGRR

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=166, Percent_Identity=32.5301204819277, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI1788099, Length=134, Percent_Identity=32.089552238806, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1786522, Length=195, Percent_Identity=32.8205128205128, Blast_Score=72, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 20811; Mature: 20811

Theoretical pI: Translated: 11.56; Mature: 11.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISAESLALFVVACVALAATPGPNVALIVGTSLKHGRRAGILTATGVNVGLIAQLALVAA
CCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCEEEECCCCHHHHHHHHHHHH
GLAYVVETFSRNFDLVRYAGAAYLVWLAVLQWRGAGAGREAPMPSARAAFGRGLAVAFAN
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCEEEEECC
PKTLLFHAAFLPQFITDRADPVPQIALLAAVFAAIALVGDVLWAVVADKARAALAGRFTR
CHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IADRVSAGILVGGAALLLAAGRR
HHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MISAESLALFVVACVALAATPGPNVALIVGTSLKHGRRAGILTATGVNVGLIAQLALVAA
CCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCEEEECCCCHHHHHHHHHHHH
GLAYVVETFSRNFDLVRYAGAAYLVWLAVLQWRGAGAGREAPMPSARAAFGRGLAVAFAN
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCEEEEECC
PKTLLFHAAFLPQFITDRADPVPQIALLAAVFAAIALVGDVLWAVVADKARAALAGRFTR
CHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IADRVSAGILVGGAALLLAAGRR
HHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]