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The map label for this gene is hemB [H]

Identifier: 154248215

GI number: 154248215

Start: 4762551

End: 4763633

Strand: Reverse

Name: hemB [H]

Synonym: Xaut_4295

Alternate gene names: 154248215

Gene position: 4763633-4762551 (Counterclockwise)

Preceding gene: 154248219

Following gene: 154248214

Centisome position: 89.73

GC content: 68.33

Gene sequence:

>1083_bases
TTGAACAACGAGCGCATCATGGCCTTCACCACGCCCCGCATCCGTCCCGTTTCCGAAGCCGAGCTGGCCCGGACCCAGGG
TCTCGACCTCCAAGGCCTCGACCTGGTCCACCGCCCCCGGCGCAACCGCAAGACCGACTGGGCGCGGCGGCTGGTGCGTG
AGAACACCCTCACCGTGGACGACCTGATCTGGCCGATCTTCGTGATGAACGGCACCGCCACCCGCGCCCCCATCCCCTCC
ATGCCGGGGGTGGAGCGCCTGACCGTGGACGAGGCGGTCCGCGCGGCCGAGATGGCCGCCAAGCTGAAGATCCCCGCGAT
CGCCATCTTCCCCTATGTGGACCCGAGCCTGCGCGATCCCCTGGGCAGCGAGGCGCTGAACAGCAACAACCTGGTTTGCC
AGACCCTGCGCGCCATCAAGGCGGAGATCCCCGAGATCGGGCTGATCACCGACGTGGCGCTGGACCCTTTCACCAGCCAC
GGCCATGACGGCCTGCTGGAGGACGGGCGCATCCTCAATGACGAGACCGTGGAAATCCTGGTGCGGCAGTCGGTGGTGCA
GGCGCAGGCGGGGGCGGACGTGATCGCACCCTCCGACATGATGGACGGCCGCATCGGCGCCATCCGCGCCGGGCTGGACG
CGGCGGGCTTCACCGATGTGCAGATCATGTCCTACGCTGCCAAGTATGCGTCCGCCTTCTACGGCCCGTTCCGCGACGCC
ATCGGCACCGCCAAGACCCTTTCCGGCGACAAGCGCACCTACCAGATGGACCCCGGCAACGGCCTGGAAGCCATCCGCGA
GGCCGCCCTCGACGTGGAGGAAGGCGCCGACATGCTCATGGTGAAGCCGGGCCTGCCCTATCTCGACATCGTCTACCGCC
TCAAGGAGACCTTCGGCCTGCCCACCTTCGCCTATCAGGTGTCCGGCGAGTACGCGATGATCGAGGGCGCCATCCGCAAC
GGCTGGGTGGACGGTGACCGCATCGTCACCGAGAGCCTGCTCGCCTTCAAGCGCGCCGGCGCCGACGGCATCCTGACCTA
TTTCGCGCCGCGCGTTGCCGAGCGGCTGGCCGCGCAGGGCTAG

Upstream 100 bases:

>100_bases
CACCGTGCGCGGCTTCACATCCCTGCCCGGCATTCCCGCCCCTCGCCGGTCAGTGCCCGGGGGGGTATAAGGATCCCCGC
GCGCCCGCAGACGGGCGCCC

Downstream 100 bases:

>100_bases
AGCGCTTCACGCTCCAGCTTTTCGAGCTTGCGCATTTTCGTCACGCGGACCGGAGTCCCCCTCGCTTGAAAATGCTCAAA
GCGCCCGCGCCGGGAGAGAC

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MNNERIMAFTTPRIRPVSEAELARTQGLDLQGLDLVHRPRRNRKTDWARRLVRENTLTVDDLIWPIFVMNGTATRAPIPS
MPGVERLTVDEAVRAAEMAAKLKIPAIAIFPYVDPSLRDPLGSEALNSNNLVCQTLRAIKAEIPEIGLITDVALDPFTSH
GHDGLLEDGRILNDETVEILVRQSVVQAQAGADVIAPSDMMDGRIGAIRAGLDAAGFTDVQIMSYAAKYASAFYGPFRDA
IGTAKTLSGDKRTYQMDPGNGLEAIREAALDVEEGADMLMVKPGLPYLDIVYRLKETFGLPTFAYQVSGEYAMIEGAIRN
GWVDGDRIVTESLLAFKRAGADGILTYFAPRVAERLAAQG

Sequences:

>Translated_360_residues
MNNERIMAFTTPRIRPVSEAELARTQGLDLQGLDLVHRPRRNRKTDWARRLVRENTLTVDDLIWPIFVMNGTATRAPIPS
MPGVERLTVDEAVRAAEMAAKLKIPAIAIFPYVDPSLRDPLGSEALNSNNLVCQTLRAIKAEIPEIGLITDVALDPFTSH
GHDGLLEDGRILNDETVEILVRQSVVQAQAGADVIAPSDMMDGRIGAIRAGLDAAGFTDVQIMSYAAKYASAFYGPFRDA
IGTAKTLSGDKRTYQMDPGNGLEAIREAALDVEEGADMLMVKPGLPYLDIVYRLKETFGLPTFAYQVSGEYAMIEGAIRN
GWVDGDRIVTESLLAFKRAGADGILTYFAPRVAERLAAQG
>Mature_360_residues
MNNERIMAFTTPRIRPVSEAELARTQGLDLQGLDLVHRPRRNRKTDWARRLVRENTLTVDDLIWPIFVMNGTATRAPIPS
MPGVERLTVDEAVRAAEMAAKLKIPAIAIFPYVDPSLRDPLGSEALNSNNLVCQTLRAIKAEIPEIGLITDVALDPFTSH
GHDGLLEDGRILNDETVEILVRQSVVQAQAGADVIAPSDMMDGRIGAIRAGLDAAGFTDVQIMSYAAKYASAFYGPFRDA
IGTAKTLSGDKRTYQMDPGNGLEAIREAALDVEEGADMLMVKPGLPYLDIVYRLKETFGLPTFAYQVSGEYAMIEGAIRN
GWVDGDRIVTESLLAFKRAGADGILTYFAPRVAERLAAQG

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen. Required for nodule development [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=308, Percent_Identity=42.5324675324675, Blast_Score=219, Evalue=3e-57,
Organism=Escherichia coli, GI87081728, Length=322, Percent_Identity=47.8260869565217, Blast_Score=269, Evalue=2e-73,
Organism=Saccharomyces cerevisiae, GI6321398, Length=314, Percent_Identity=39.4904458598726, Blast_Score=189, Evalue=8e-49,
Organism=Drosophila melanogaster, GI21358291, Length=291, Percent_Identity=39.1752577319588, Blast_Score=221, Evalue=8e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 39318; Mature: 39318

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNERIMAFTTPRIRPVSEAELARTQGLDLQGLDLVHRPRRNRKTDWARRLVRENTLTVD
CCCCEEEEEECCCCCCCCHHHHHHHCCCCCCCCHHHHCCCCCCCHHHHHHHHHCCCCCHH
DLIWPIFVMNGTATRAPIPSMPGVERLTVDEAVRAAEMAAKLKIPAIAIFPYVDPSLRDP
HHHHHHHEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCHHCCC
LGSEALNSNNLVCQTLRAIKAEIPEIGLITDVALDPFTSHGHDGLLEDGRILNDETVEIL
CCCHHCCCCCHHHHHHHHHHHCCCCCCEEEHHHCCCCCCCCCCCCCCCCEEECCHHHHHH
VRQSVVQAQAGADVIAPSDMMDGRIGAIRAGLDAAGFTDVQIMSYAAKYASAFYGPFRDA
HHHHHHHHHCCCCCCCCCHHCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IGTAKTLSGDKRTYQMDPGNGLEAIREAALDVEEGADMLMVKPGLPYLDIVYRLKETFGL
HCCHHHCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCC
PTFAYQVSGEYAMIEGAIRNGWVDGDRIVTESLLAFKRAGADGILTYFAPRVAERLAAQG
CEEEEEECCCEEEEEHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNNERIMAFTTPRIRPVSEAELARTQGLDLQGLDLVHRPRRNRKTDWARRLVRENTLTVD
CCCCEEEEEECCCCCCCCHHHHHHHCCCCCCCCHHHHCCCCCCCHHHHHHHHHCCCCCHH
DLIWPIFVMNGTATRAPIPSMPGVERLTVDEAVRAAEMAAKLKIPAIAIFPYVDPSLRDP
HHHHHHHEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCHHCCC
LGSEALNSNNLVCQTLRAIKAEIPEIGLITDVALDPFTSHGHDGLLEDGRILNDETVEIL
CCCHHCCCCCHHHHHHHHHHHCCCCCCEEEHHHCCCCCCCCCCCCCCCCEEECCHHHHHH
VRQSVVQAQAGADVIAPSDMMDGRIGAIRAGLDAAGFTDVQIMSYAAKYASAFYGPFRDA
HHHHHHHHHCCCCCCCCCHHCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IGTAKTLSGDKRTYQMDPGNGLEAIREAALDVEEGADMLMVKPGLPYLDIVYRLKETFGL
HCCHHHCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCC
PTFAYQVSGEYAMIEGAIRNGWVDGDRIVTESLLAFKRAGADGILTYFAPRVAERLAAQG
CEEEEEECCCEEEEEHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8226669; 12597275 [H]