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The map label for this gene is glyA [H]

Identifier: 154248196

GI number: 154248196

Start: 4742827

End: 4744182

Strand: Direct

Name: glyA [H]

Synonym: Xaut_4276

Alternate gene names: 154248196

Gene position: 4742827-4744182 (Clockwise)

Preceding gene: 154248195

Following gene: 154248197

Centisome position: 89.34

GC content: 66.89

Gene sequence:

>1356_bases
ATGCGTCCCGCCGGGGTGCCCCGACATTACCGGAGACCTGCCATGGCCTCGCAGGACGCGAACCGCTCCTCCATTTCGGA
GGAAATGAACCGCTTCTTCACTGCCCCTCTCGCCGAGAGCGATCCCGAGATCGCCGGCGCGGTGAAGGCGGAGCTGGGTC
GCCAGCGCGACGAGATCGAGCTGATCGCGTCCGAGAACATCGTCTCGCGCGCCGTGCTCGAGGCCCAGGGCTCCGTGCTC
ACCAACAAGTATGCCGAGGGCTATCCGGGCAAGCGCTACTACGGCGGCTGCCAGTTCGTGGACGTGGCGGAAAACCTCGC
CATCGAGCGCGCCAAGAAGCTGTTCGGCTGCGGCTTCGCCAACGTCCAGCCCAATTCCGGCAGCCAGGCCAACCAGGGCG
TGTTCTTCGCCCTGCTGCAGCCGGGCGATACCTTTCTGGGCCTCAACCTTGCCGCCGGCGGTCACCTGACCCACGGCTCG
CCGGTCAACATGTCCGGCAAGTGGTTCAAGCCCGTGCCCTACACGGTGCGCGAGGACGATCAGCGCATCGACTATGACGA
GGTGGCGCGGCTCGCCGACGAGCACAAGCCCAAGCTCATCGTGGCCGGCGGCTCGGCCTACCCGCGCGTCATCGATTTCC
CGAAGATGCGCGCCATCGCCGACAGCGTGGGCGCCAAGCTCATGGTGGACATGGCGCATTTTGCCGGTCTCGTCGCCGGT
GGCGCCCACCCGAGCCCGTTCCCCCACGCCCATGTGGTGACCACCACCACCCACAAGACCCTGCGTGGGCCGCGCGGCGG
CATGATCCTCACCAATGACGAGGACCTGGCCAAGAAGATCAATTCGGCCATCTTCCCCGGCATCCAGGGCGGGCCGCTGA
TGCATGTCATCGCTGCCAAGGCGGTGGCCTTCGGCGAGGCGCTGCGCCCCGAGTTCAAGCTCTATGCGAAGAACGTGGTG
GAGAATGCCAAGGCGCTGGCCGAGACCCTGAAGGGCCACGGCTTCAACATCGTCTCCGGTGGCACCGACACCCACCTGAT
GCTGGTGGACCTGCGCCCCAAGCGCCTCACCGGCAAGACCTCGGAAGGCGCGCTGGGCCGGGCGCACATCACCACCAACA
AGAACGGCATCCCGTTCGATCCCGAGAAGCCGTTCGTGACCTCCGGCATCCGCCTCGGCACGCCGGCCTGCACCACGCGC
GGCTTCGGGGTGGCCGAGTTCCAGCAGGTGGGCGACTTCATCGCCGAGGTGCTCGACGTGCTCTCCCAGAAGGGCGTGGA
CGAGGATTCGCTGGTGGAAGCCACGGTGCGTGAGAAGGTCTCGGGCCTGCTCGCCCGCTTCCCCATCTATCCCTGA

Upstream 100 bases:

>100_bases
GCCGGCGCAGGGCTGCGACGCTGCTGCCGCATGCCCGGGCTTTGGACTTCACCTCGACCCTGTGATAAGCGCAGGGTCCG
CTCCCGAGGGCCGCTTGAAC

Downstream 100 bases:

>100_bases
GGGCAAGTCCCCGGAGGGGCAAGTGCCAGCGCGTCTTGGTCTCGCGGACCGGTGTCCCCGTGCGAAGACGCCTGAGCGCC
GGAAAGGGTGAAGCATGCGC

Product: glycine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase [H]

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MRPAGVPRHYRRPAMASQDANRSSISEEMNRFFTAPLAESDPEIAGAVKAELGRQRDEIELIASENIVSRAVLEAQGSVL
TNKYAEGYPGKRYYGGCQFVDVAENLAIERAKKLFGCGFANVQPNSGSQANQGVFFALLQPGDTFLGLNLAAGGHLTHGS
PVNMSGKWFKPVPYTVREDDQRIDYDEVARLADEHKPKLIVAGGSAYPRVIDFPKMRAIADSVGAKLMVDMAHFAGLVAG
GAHPSPFPHAHVVTTTTHKTLRGPRGGMILTNDEDLAKKINSAIFPGIQGGPLMHVIAAKAVAFGEALRPEFKLYAKNVV
ENAKALAETLKGHGFNIVSGGTDTHLMLVDLRPKRLTGKTSEGALGRAHITTNKNGIPFDPEKPFVTSGIRLGTPACTTR
GFGVAEFQQVGDFIAEVLDVLSQKGVDEDSLVEATVREKVSGLLARFPIYP

Sequences:

>Translated_451_residues
MRPAGVPRHYRRPAMASQDANRSSISEEMNRFFTAPLAESDPEIAGAVKAELGRQRDEIELIASENIVSRAVLEAQGSVL
TNKYAEGYPGKRYYGGCQFVDVAENLAIERAKKLFGCGFANVQPNSGSQANQGVFFALLQPGDTFLGLNLAAGGHLTHGS
PVNMSGKWFKPVPYTVREDDQRIDYDEVARLADEHKPKLIVAGGSAYPRVIDFPKMRAIADSVGAKLMVDMAHFAGLVAG
GAHPSPFPHAHVVTTTTHKTLRGPRGGMILTNDEDLAKKINSAIFPGIQGGPLMHVIAAKAVAFGEALRPEFKLYAKNVV
ENAKALAETLKGHGFNIVSGGTDTHLMLVDLRPKRLTGKTSEGALGRAHITTNKNGIPFDPEKPFVTSGIRLGTPACTTR
GFGVAEFQQVGDFIAEVLDVLSQKGVDEDSLVEATVREKVSGLLARFPIYP
>Mature_451_residues
MRPAGVPRHYRRPAMASQDANRSSISEEMNRFFTAPLAESDPEIAGAVKAELGRQRDEIELIASENIVSRAVLEAQGSVL
TNKYAEGYPGKRYYGGCQFVDVAENLAIERAKKLFGCGFANVQPNSGSQANQGVFFALLQPGDTFLGLNLAAGGHLTHGS
PVNMSGKWFKPVPYTVREDDQRIDYDEVARLADEHKPKLIVAGGSAYPRVIDFPKMRAIADSVGAKLMVDMAHFAGLVAG
GAHPSPFPHAHVVTTTTHKTLRGPRGGMILTNDEDLAKKINSAIFPGIQGGPLMHVIAAKAVAFGEALRPEFKLYAKNVV
ENAKALAETLKGHGFNIVSGGTDTHLMLVDLRPKRLTGKTSEGALGRAHITTNKNGIPFDPEKPFVTSGIRLGTPACTTR
GFGVAEFQQVGDFIAEVLDVLSQKGVDEDSLVEATVREKVSGLLARFPIYP

Specific function: Interconversion of serine and glycine [H]

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family [H]

Homologues:

Organism=Homo sapiens, GI19923315, Length=435, Percent_Identity=46.6666666666667, Blast_Score=353, Evalue=1e-97,
Organism=Homo sapiens, GI261862352, Length=433, Percent_Identity=46.6512702078522, Blast_Score=352, Evalue=3e-97,
Organism=Homo sapiens, GI261862350, Length=433, Percent_Identity=46.6512702078522, Blast_Score=352, Evalue=3e-97,
Organism=Homo sapiens, GI261862348, Length=433, Percent_Identity=46.6512702078522, Blast_Score=352, Evalue=3e-97,
Organism=Homo sapiens, GI22547186, Length=446, Percent_Identity=46.8609865470852, Blast_Score=344, Evalue=1e-94,
Organism=Homo sapiens, GI261862346, Length=435, Percent_Identity=46.2068965517241, Blast_Score=342, Evalue=6e-94,
Organism=Homo sapiens, GI22547189, Length=432, Percent_Identity=44.6759259259259, Blast_Score=309, Evalue=4e-84,
Organism=Escherichia coli, GI1788902, Length=428, Percent_Identity=58.8785046728972, Blast_Score=478, Evalue=1e-136,
Organism=Caenorhabditis elegans, GI25144732, Length=402, Percent_Identity=48.5074626865672, Blast_Score=352, Evalue=2e-97,
Organism=Caenorhabditis elegans, GI25144729, Length=402, Percent_Identity=48.5074626865672, Blast_Score=352, Evalue=2e-97,
Organism=Saccharomyces cerevisiae, GI6319739, Length=404, Percent_Identity=46.2871287128713, Blast_Score=355, Evalue=1e-98,
Organism=Saccharomyces cerevisiae, GI6323087, Length=465, Percent_Identity=41.7204301075269, Blast_Score=344, Evalue=1e-95,
Organism=Drosophila melanogaster, GI24640005, Length=432, Percent_Identity=45.8333333333333, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI221329721, Length=413, Percent_Identity=46.9733656174334, Blast_Score=360, Evalue=1e-99,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798 [H]

Pfam domain/function: PF00464 SHMT [H]

EC number: =2.1.2.1 [H]

Molecular weight: Translated: 48497; Mature: 48497

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPAGVPRHYRRPAMASQDANRSSISEEMNRFFTAPLAESDPEIAGAVKAELGRQRDEIE
CCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCEE
LIASENIVSRAVLEAQGSVLTNKYAEGYPGKRYYGGCQFVDVAENLAIERAKKLFGCGFA
EEECCHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHCCCCC
NVQPNSGSQANQGVFFALLQPGDTFLGLNLAAGGHLTHGSPVNMSGKWFKPVPYTVREDD
CCCCCCCCCCCCCEEEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCEECCCCCCCCCCC
QRIDYDEVARLADEHKPKLIVAGGSAYPRVIDFPKMRAIADSVGAKLMVDMAHFAGLVAG
CCCCHHHHHHHHHCCCCEEEEECCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHCC
GAHPSPFPHAHVVTTTTHKTLRGPRGGMILTNDEDLAKKINSAIFPGIQGGPLMHVIAAK
CCCCCCCCCCEEEEECCHHHHCCCCCCEEEECCHHHHHHHHHHHCCCCCCCHHHHHHHHH
AVAFGEALRPEFKLYAKNVVENAKALAETLKGHGFNIVSGGTDTHLMLVDLRPKRLTGKT
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEECCCCCCCCCC
SEGALGRAHITTNKNGIPFDPEKPFVTSGIRLGTPACTTRGFGVAEFQQVGDFIAEVLDV
CCCCCCEEEEEECCCCCCCCCCCCHHCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHH
LSQKGVDEDSLVEATVREKVSGLLARFPIYP
HHHCCCCHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRPAGVPRHYRRPAMASQDANRSSISEEMNRFFTAPLAESDPEIAGAVKAELGRQRDEIE
CCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCEE
LIASENIVSRAVLEAQGSVLTNKYAEGYPGKRYYGGCQFVDVAENLAIERAKKLFGCGFA
EEECCHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHCCCCC
NVQPNSGSQANQGVFFALLQPGDTFLGLNLAAGGHLTHGSPVNMSGKWFKPVPYTVREDD
CCCCCCCCCCCCCEEEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCEECCCCCCCCCCC
QRIDYDEVARLADEHKPKLIVAGGSAYPRVIDFPKMRAIADSVGAKLMVDMAHFAGLVAG
CCCCHHHHHHHHHCCCCEEEEECCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHCC
GAHPSPFPHAHVVTTTTHKTLRGPRGGMILTNDEDLAKKINSAIFPGIQGGPLMHVIAAK
CCCCCCCCCCEEEEECCHHHHCCCCCCEEEECCHHHHHHHHHHHCCCCCCCHHHHHHHHH
AVAFGEALRPEFKLYAKNVVENAKALAETLKGHGFNIVSGGTDTHLMLVDLRPKRLTGKT
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEECCCCCCCCCC
SEGALGRAHITTNKNGIPFDPEKPFVTSGIRLGTPACTTRGFGVAEFQQVGDFIAEVLDV
CCCCCCEEEEEECCCCCCCCCCCCHHCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHH
LSQKGVDEDSLVEATVREKVSGLLARFPIYP
HHHCCCCHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA