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The map label for this gene is dusA [H]

Identifier: 154248170

GI number: 154248170

Start: 4715089

End: 4716081

Strand: Reverse

Name: dusA [H]

Synonym: Xaut_4250

Alternate gene names: 154248170

Gene position: 4716081-4715089 (Counterclockwise)

Preceding gene: 154248174

Following gene: 154248169

Centisome position: 88.83

GC content: 69.28

Gene sequence:

>993_bases
ATGATGCAGTCACAGCCGTTTAGATTTTCAGTCGCCCCCATGATGGATTGGACCGACCGCCATTGCCGGGCGTTCCACCG
CACTCTTTCGCGCCGGGCGCGGCTCTATACGGAGATGGTCACGGCGCCGGCCATCCTCCATGGCGATCGCGCGCGCCTGC
TCGGGTTCTCGCCGGAGGAGCACCCGGTGGCGGTTCAGCTGGGCGGCAGCGATCCGGCGGAGCTGGCGCAGGCGGCGCGG
ATCTGCGCCGATTATGGCTATGACGAGATCAACCTCAACGTGGGTTGCCCCTCCGACCGGGTTCAGGGTGGCAATTTTGG
CGCCTGCCTCATGCGCGAGCCAGACCTCGTGGCCGATTGCGTCGCCGCCATGAAGGCGGTCGTGGCGATCCCCGTCACCG
TCAAGTGCCGCATCGGCGTGGACGACCAGGACCCGGAGGAAGCCCTCGATCGCCTCGCCGATGCCGTGGTGGCAGCCGGC
ACCGATGCGCTCATCGTCCATGCCCGCAAGGCGTGGCTGAAGGGCCTGTCGCCGCGCGAGAACCGCGATATCCCGCCTCT
CGACTATGACCGGGTTCGCCGGCTGAAGGCCCGCCTGCCCCACGTGCCCATCGCGGTGAATGGCGGCATCGCCAGCATCG
CCCAGGGACTGGAGGAGGCGCGCGGTCTCGACGGCATGATGCTGGGTCGCGCCGCCTATCAGGATCCCGAACTACTGCTC
GGCGTCGATCCGCTACTGTTCGGCACGCCTGCCCCCCATGCAGACGGCTTCGCCGCGGTGGAGGCCTTCGAGCCCTATGT
GGCGCACCATCTGGAGGCCGGCGGGCGGCTCTCCGACATCACCCGCCACATGCTGGGCCTGTTCACCGGCCGCAAGGGCG
CGCGCATCTTCCGCCGCCATCTCTCCACCGAGGCCATCCGCCCCGGCGCCGGGCTCGACGTGCTGCGCGCGGCCGTGGAT
GAGGTACGCCGGCGCCAGCCTCTGGCGGCCTGA

Upstream 100 bases:

>100_bases
CGCCGTATGGCGGAAGCGTCGGAAACCGGAGAATTGGGCGAGCCACGGCGCCGGAGCGAATGCAAATCGCGCGTCGCCGT
CCTGACAAGCCGTACAGAAA

Downstream 100 bases:

>100_bases
TCGCCCTTTCCTTCCGGTCTGATCTGCACGCCATGCGCGGCTTCCGAACGCGCGTATGCGGGCGCGATGGTGGACAGGGA
GGCTGATCCGCGCCACTGCT

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MMQSQPFRFSVAPMMDWTDRHCRAFHRTLSRRARLYTEMVTAPAILHGDRARLLGFSPEEHPVAVQLGGSDPAELAQAAR
ICADYGYDEINLNVGCPSDRVQGGNFGACLMREPDLVADCVAAMKAVVAIPVTVKCRIGVDDQDPEEALDRLADAVVAAG
TDALIVHARKAWLKGLSPRENRDIPPLDYDRVRRLKARLPHVPIAVNGGIASIAQGLEEARGLDGMMLGRAAYQDPELLL
GVDPLLFGTPAPHADGFAAVEAFEPYVAHHLEAGGRLSDITRHMLGLFTGRKGARIFRRHLSTEAIRPGAGLDVLRAAVD
EVRRRQPLAA

Sequences:

>Translated_330_residues
MMQSQPFRFSVAPMMDWTDRHCRAFHRTLSRRARLYTEMVTAPAILHGDRARLLGFSPEEHPVAVQLGGSDPAELAQAAR
ICADYGYDEINLNVGCPSDRVQGGNFGACLMREPDLVADCVAAMKAVVAIPVTVKCRIGVDDQDPEEALDRLADAVVAAG
TDALIVHARKAWLKGLSPRENRDIPPLDYDRVRRLKARLPHVPIAVNGGIASIAQGLEEARGLDGMMLGRAAYQDPELLL
GVDPLLFGTPAPHADGFAAVEAFEPYVAHHLEAGGRLSDITRHMLGLFTGRKGARIFRRHLSTEAIRPGAGLDVLRAAVD
EVRRRQPLAA
>Mature_330_residues
MMQSQPFRFSVAPMMDWTDRHCRAFHRTLSRRARLYTEMVTAPAILHGDRARLLGFSPEEHPVAVQLGGSDPAELAQAAR
ICADYGYDEINLNVGCPSDRVQGGNFGACLMREPDLVADCVAAMKAVVAIPVTVKCRIGVDDQDPEEALDRLADAVVAAG
TDALIVHARKAWLKGLSPRENRDIPPLDYDRVRRLKARLPHVPIAVNGGIASIAQGLEEARGLDGMMLGRAAYQDPELLL
GVDPLLFGTPAPHADGFAAVEAFEPYVAHHLEAGGRLSDITRHMLGLFTGRKGARIFRRHLSTEAIRPGAGLDVLRAAVD
EVRRRQPLAA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=316, Percent_Identity=28.4810126582279, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI40807366, Length=255, Percent_Identity=29.8039215686275, Blast_Score=79, Evalue=6e-15,
Organism=Escherichia coli, GI145693211, Length=316, Percent_Identity=57.5949367088608, Blast_Score=358, Evalue=1e-100,
Organism=Escherichia coli, GI1789660, Length=305, Percent_Identity=28.1967213114754, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1788462, Length=186, Percent_Identity=33.3333333333333, Blast_Score=84, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI25144369, Length=229, Percent_Identity=29.6943231441048, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17507177, Length=279, Percent_Identity=26.1648745519713, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323560, Length=234, Percent_Identity=28.2051282051282, Blast_Score=79, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24580595, Length=255, Percent_Identity=27.843137254902, Blast_Score=88, Evalue=8e-18,
Organism=Drosophila melanogaster, GI19920448, Length=255, Percent_Identity=27.843137254902, Blast_Score=88, Evalue=8e-18,
Organism=Drosophila melanogaster, GI19921524, Length=243, Percent_Identity=27.1604938271605, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 35910; Mature: 35910

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMQSQPFRFSVAPMMDWTDRHCRAFHRTLSRRARLYTEMVTAPAILHGDRARLLGFSPEE
CCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEECCCCCC
HPVAVQLGGSDPAELAQAARICADYGYDEINLNVGCPSDRVQGGNFGACLMREPDLVADC
CCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCEEEECCCHHHHHH
VAAMKAVVAIPVTVKCRIGVDDQDPEEALDRLADAVVAAGTDALIVHARKAWLKGLSPRE
HHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEHHHHHHHHCCCCCCC
NRDIPPLDYDRVRRLKARLPHVPIAVNGGIASIAQGLEEARGLDGMMLGRAAYQDPELLL
CCCCCCCCHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHCCCCCHHHHHHHCCCCCEEE
GVDPLLFGTPAPHADGFAAVEAFEPYVAHHLEAGGRLSDITRHMLGLFTGRKGARIFRRH
ECCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHH
LSTEAIRPGAGLDVLRAAVDEVRRRQPLAA
HHHHHCCCCCCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MMQSQPFRFSVAPMMDWTDRHCRAFHRTLSRRARLYTEMVTAPAILHGDRARLLGFSPEE
CCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEECCCCCC
HPVAVQLGGSDPAELAQAARICADYGYDEINLNVGCPSDRVQGGNFGACLMREPDLVADC
CCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCEEEECCCHHHHHH
VAAMKAVVAIPVTVKCRIGVDDQDPEEALDRLADAVVAAGTDALIVHARKAWLKGLSPRE
HHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEHHHHHHHHCCCCCCC
NRDIPPLDYDRVRRLKARLPHVPIAVNGGIASIAQGLEEARGLDGMMLGRAAYQDPELLL
CCCCCCCCHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHCCCCCHHHHHHHCCCCCEEE
GVDPLLFGTPAPHADGFAAVEAFEPYVAHHLEAGGRLSDITRHMLGLFTGRKGARIFRRH
ECCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHH
LSTEAIRPGAGLDVLRAAVDEVRRRQPLAA
HHHHHCCCCCCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]