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The map label for this gene is gloA [H]

Identifier: 154248092

GI number: 154248092

Start: 4620056

End: 4620478

Strand: Reverse

Name: gloA [H]

Synonym: Xaut_4172

Alternate gene names: 154248092

Gene position: 4620478-4620056 (Counterclockwise)

Preceding gene: 154248096

Following gene: 154248091

Centisome position: 87.03

GC content: 66.19

Gene sequence:

>423_bases
ATGCGCTTTCTGCACACCATGGTCCGCGTGACCGACATCGACCAGTCGCTGGATTTCTATTGCAACAAGCTGGGGCTGAA
GGAGGTCCGCCGCAAGGAGGTGCCGCAGGGACGGTACACCCTCGTCTTCCTCGCCGCCCCCGGCCAGGCCGATGTGGCCG
AGATCGAGCTGACCTACAACTGGGATCCCGAGCCCTATGGCGAGGGCCGCAATTTCGGCCATCTCGCCTTCGAGGTGGAG
GACATCTACGCCACCTGCGAGCGCCTGATGGCGGGCGGCGTCACCATCAACCGGCCGCCGCGCGACGGCTACATGGCCTT
CGTCCGCTCGCCCGACAACGTGTCGGTGGAGCTGCTGCAGAAAGGCGGCCCCAAAGCTCCGGCCGAGCCCTGGGCCTCCA
TGGCCAACACCGGCAAGTGGTGA

Upstream 100 bases:

>100_bases
CGGCGCCCTCCACTTGCCCAAACGGCGCCGGCTACGCCGGCCGGCGCGGACCCTATCCCCATCCGGAGCGCCGCGCTCCC
TCTGAACGACGAGAACGCCA

Downstream 100 bases:

>100_bases
GCTGAAGGGCGGAACCGAGCTCCGGCGCCGCTTGGCGGACAGGCCGCCCTTCTGTTTGGGCAGCCTCCTTTCGGCAAGCT
TGCACATGGCCAGAGGAGGC

Product: glyoxalase/bleomycin resistance protein/dioxygenase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 140; Mature: 140

Protein sequence:

>140_residues
MRFLHTMVRVTDIDQSLDFYCNKLGLKEVRRKEVPQGRYTLVFLAAPGQADVAEIELTYNWDPEPYGEGRNFGHLAFEVE
DIYATCERLMAGGVTINRPPRDGYMAFVRSPDNVSVELLQKGGPKAPAEPWASMANTGKW

Sequences:

>Translated_140_residues
MRFLHTMVRVTDIDQSLDFYCNKLGLKEVRRKEVPQGRYTLVFLAAPGQADVAEIELTYNWDPEPYGEGRNFGHLAFEVE
DIYATCERLMAGGVTINRPPRDGYMAFVRSPDNVSVELLQKGGPKAPAEPWASMANTGKW
>Mature_140_residues
MRFLHTMVRVTDIDQSLDFYCNKLGLKEVRRKEVPQGRYTLVFLAAPGQADVAEIELTYNWDPEPYGEGRNFGHLAFEVE
DIYATCERLMAGGVTINRPPRDGYMAFVRSPDNVSVELLQKGGPKAPAEPWASMANTGKW

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=143, Percent_Identity=32.8671328671329, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI1787940, Length=126, Percent_Identity=38.8888888888889, Blast_Score=99, Evalue=9e-23,
Organism=Saccharomyces cerevisiae, GI6323639, Length=144, Percent_Identity=31.9444444444444, Blast_Score=71, Evalue=5e-14,
Organism=Drosophila melanogaster, GI20129743, Length=144, Percent_Identity=34.0277777777778, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 15797; Mature: 15797

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00934 GLYOXALASE_I_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFLHTMVRVTDIDQSLDFYCNKLGLKEVRRKEVPQGRYTLVFLAAPGQADVAEIELTYN
CCHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHCCCCCCEEEEEEECCCCCCEEEEEEEEC
WDPEPYGEGRNFGHLAFEVEDIYATCERLMAGGVTINRPPRDGYMAFVRSPDNVSVELLQ
CCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHCCEEECCCCCCCEEEEEECCCCCEEEEEE
KGGPKAPAEPWASMANTGKW
CCCCCCCCCHHHHHHCCCCC
>Mature Secondary Structure
MRFLHTMVRVTDIDQSLDFYCNKLGLKEVRRKEVPQGRYTLVFLAAPGQADVAEIELTYN
CCHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHCCCCCCEEEEEEECCCCCCEEEEEEEEC
WDPEPYGEGRNFGHLAFEVEDIYATCERLMAGGVTINRPPRDGYMAFVRSPDNVSVELLQ
CCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHCCEEECCCCCCCEEEEEECCCCCEEEEEE
KGGPKAPAEPWASMANTGKW
CCCCCCCCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]