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The map label for this gene is yitF [H]

Identifier: 154247298

GI number: 154247298

Start: 3756585

End: 3757754

Strand: Reverse

Name: yitF [H]

Synonym: Xaut_3370

Alternate gene names: 154247298

Gene position: 3757754-3756585 (Counterclockwise)

Preceding gene: 154247299

Following gene: 154247297

Centisome position: 70.78

GC content: 68.12

Gene sequence:

>1170_bases
GTGAGCACTGAAAAAGGTGAACAGGCGGCGCCCATGAAGATCGCGGCGGTCCACACCCACCTTCTCGACCATCGGCTCGA
CGTGCCGTTCGAGAGCGCATCCATGCGCTTCGACCGTCGCACCCATGTGCTGGTCGAGATCGTGTGCGACGATGGCACCA
CGGGCTGGGGCGAGTGCCTCGGCCCGGCGCGGCCCAATGCGGCGGTGGTGGCGGCCTATGCCAACTGGCTCATCGGCGCC
GATCCGCTGGAAACCGAGAAGATCTGGGCGACCCTCTACAACGCCCTGCGCGATCAGGGCCAGCGCGGCCTCACCGTCAC
TGCGCTGTCCGGCATCGATATCGCGCTGTGGGACATCAAGGGCAAGCATTTCGGCGTGTCGGTGGCCACGCTCCTGGGCG
GCCGCTTCCGCGAGAGCGTGCGCGCCTACGCCACCGGCAGCTTCGTGCGGGACGGGGTCGACCGCGTCGCCGACAATGCC
ACCGAGATGGCAACCCATGCCGCCGCCGGCTTCCACGCCGCGAAGATCAAGATCGGCTTCGGCGTGGACGCCGACCTGAA
GGTGCTCAAGGCCGTGCGCGAGGCCGTCGGCCCGGACATGCGCCTGATGGTGGATGCCAACCACGGCTATGACGCGTTGG
AAGCCATACAGTTCGGCAATCGGGCGGTTGAGTATGGCGTCGACTGGTTCGAGGAGCCGGTGGTTCCCGAGCAGCTTGCC
GCCTACCGGGCGGTGAAGGCCGGCCAGCCCATCCCCGTGGCCGGTGGCGAAACCTGGCACACCCGCTGGGGCATGCGCGA
GCCCATCGAGACCCGGGCCGTGGACATCATCCAGCCGGACCTGTGCGGTGTGGGCGGCTTCACCGAGGCCAAGCGCGTGG
CCGACCTTGCCGCGCTCCACGGGGTGCGTGTGGTGCCGCACGTGTGGGGCACGGCGGTGCAGATCGCGGCCGCCCTGCAA
TTCATGGCCGCCATGGTGCCGAACCCGGTGCGCACCCGCCCCATCGAGCCCATTCTCGAATTTGACCGCACCCACAATCC
GTTCCGCCAGGCGGTGGTGAAGACGCCGCTGGAGCATGTGAAGGGCGTCGTCGCCATTCCGGATGGACCTGGCCTCGGCA
TCGAGATCAACCGCGACGCGCTCGCCGAATTTCGTCCGAAGGAGGCCTGA

Upstream 100 bases:

>100_bases
GGCTGCAGGGATTCGCCGCCGGCCCAGTGCGTGCGCCCCTCGACGACCTCACCATTGAGGAGGAAGCCATGCTCGACGCC
CTTATCGGCACCTGGAAGCG

Downstream 100 bases:

>100_bases
CATGCTGGAGCGCAAGCTGTCGGGCCGCCCGCCCGTTCCCCCCTTCCCGCCGGGTGCGGTGGACACGCAGATGCACATGT
ATCTGCCGGGCTTCCCCGCG

Product: mandelate racemase/muconate lactonizing protein

Products: Glyceraldehyde 3-phosphate; Pyruvate [C]

Alternate protein names: NA

Number of amino acids: Translated: 389; Mature: 388

Protein sequence:

>389_residues
MSTEKGEQAAPMKIAAVHTHLLDHRLDVPFESASMRFDRRTHVLVEIVCDDGTTGWGECLGPARPNAAVVAAYANWLIGA
DPLETEKIWATLYNALRDQGQRGLTVTALSGIDIALWDIKGKHFGVSVATLLGGRFRESVRAYATGSFVRDGVDRVADNA
TEMATHAAAGFHAAKIKIGFGVDADLKVLKAVREAVGPDMRLMVDANHGYDALEAIQFGNRAVEYGVDWFEEPVVPEQLA
AYRAVKAGQPIPVAGGETWHTRWGMREPIETRAVDIIQPDLCGVGGFTEAKRVADLAALHGVRVVPHVWGTAVQIAAALQ
FMAAMVPNPVRTRPIEPILEFDRTHNPFRQAVVKTPLEHVKGVVAIPDGPGLGIEINRDALAEFRPKEA

Sequences:

>Translated_389_residues
MSTEKGEQAAPMKIAAVHTHLLDHRLDVPFESASMRFDRRTHVLVEIVCDDGTTGWGECLGPARPNAAVVAAYANWLIGA
DPLETEKIWATLYNALRDQGQRGLTVTALSGIDIALWDIKGKHFGVSVATLLGGRFRESVRAYATGSFVRDGVDRVADNA
TEMATHAAAGFHAAKIKIGFGVDADLKVLKAVREAVGPDMRLMVDANHGYDALEAIQFGNRAVEYGVDWFEEPVVPEQLA
AYRAVKAGQPIPVAGGETWHTRWGMREPIETRAVDIIQPDLCGVGGFTEAKRVADLAALHGVRVVPHVWGTAVQIAAALQ
FMAAMVPNPVRTRPIEPILEFDRTHNPFRQAVVKTPLEHVKGVVAIPDGPGLGIEINRDALAEFRPKEA
>Mature_388_residues
STEKGEQAAPMKIAAVHTHLLDHRLDVPFESASMRFDRRTHVLVEIVCDDGTTGWGECLGPARPNAAVVAAYANWLIGAD
PLETEKIWATLYNALRDQGQRGLTVTALSGIDIALWDIKGKHFGVSVATLLGGRFRESVRAYATGSFVRDGVDRVADNAT
EMATHAAAGFHAAKIKIGFGVDADLKVLKAVREAVGPDMRLMVDANHGYDALEAIQFGNRAVEYGVDWFEEPVVPEQLAA
YRAVKAGQPIPVAGGETWHTRWGMREPIETRAVDIIQPDLCGVGGFTEAKRVADLAALHGVRVVPHVWGTAVQIAAALQF
MAAMVPNPVRTRPIEPILEFDRTHNPFRQAVVKTPLEHVKGVVAIPDGPGLGIEINRDALAEFRPKEA

Specific function: D-galactonate degradation; first step. D-galactonate degradation; third step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI42544119, Length=234, Percent_Identity=24.3589743589744, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI186972148, Length=234, Percent_Identity=24.3589743589744, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI42544117, Length=218, Percent_Identity=22.0183486238532, Blast_Score=72, Evalue=7e-13,
Organism=Escherichia coli, GI48994953, Length=353, Percent_Identity=30.028328611898, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1787864, Length=393, Percent_Identity=28.7531806615776, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI226510960, Length=310, Percent_Identity=30.3225806451613, Blast_Score=113, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015654
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: 4.1.2.21; 4.2.1.6 [C]

Molecular weight: Translated: 42190; Mature: 42058

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS00908 MR_MLE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTEKGEQAAPMKIAAVHTHLLDHRLDVPFESASMRFDRRTHVLVEIVCDDGTTGWGECL
CCCCCCCCCCCEEHHHHHHHHHHHHCCCCCCHHHCCCCCCCEEEEEEEECCCCCCHHHHC
GPARPNAAVVAAYANWLIGADPLETEKIWATLYNALRDQGQRGLTVTALSGIDIALWDIK
CCCCCCCEEEEEEHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEEEEC
GKHFGVSVATLLGGRFRESVRAYATGSFVRDGVDRVADNATEMATHAAAGFHAAKIKIGF
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEEEEEC
GVDADLKVLKAVREAVGPDMRLMVDANHGYDALEAIQFGNRAVEYGVDWFEEPVVPEQLA
CCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCHHHHHCCHHHHCCCCHHHHH
AYRAVKAGQPIPVAGGETWHTRWGMREPIETRAVDIIQPDLCGVGGFTEAKRVADLAALH
HHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHC
GVRVVPHVWGTAVQIAAALQFMAAMVPNPVRTRPIEPILEFDRTHNPFRQAVVKTPLEHV
CCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCHHHHHHHHCCHHHC
KGVVAIPDGPGLGIEINRDALAEFRPKEA
CEEEECCCCCCCCEEECHHHHHCCCCCCC
>Mature Secondary Structure 
STEKGEQAAPMKIAAVHTHLLDHRLDVPFESASMRFDRRTHVLVEIVCDDGTTGWGECL
CCCCCCCCCCEEHHHHHHHHHHHHCCCCCCHHHCCCCCCCEEEEEEEECCCCCCHHHHC
GPARPNAAVVAAYANWLIGADPLETEKIWATLYNALRDQGQRGLTVTALSGIDIALWDIK
CCCCCCCEEEEEEHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEEEEC
GKHFGVSVATLLGGRFRESVRAYATGSFVRDGVDRVADNATEMATHAAAGFHAAKIKIGF
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEEEEEC
GVDADLKVLKAVREAVGPDMRLMVDANHGYDALEAIQFGNRAVEYGVDWFEEPVVPEQLA
CCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCHHHHHCCHHHHCCCCHHHHH
AYRAVKAGQPIPVAGGETWHTRWGMREPIETRAVDIIQPDLCGVGGFTEAKRVADLAALH
HHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHC
GVRVVPHVWGTAVQIAAALQFMAAMVPNPVRTRPIEPILEFDRTHNPFRQAVVKTPLEHV
CCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCHHHHHHHHCCHHHC
KGVVAIPDGPGLGIEINRDALAEFRPKEA
CEEEECCCCCCCCEEECHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-Dehydro-3-deoxy-D-galactonate 6-phosphate [C]

Specific reaction: 2-Dehydro-3-deoxy-D-galactonate 6-phosphate <==> Glyceraldehyde 3-phosphate + Pyruvate [C]

General reaction: elimination of H2O C-O bond; Cleavage [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353932; 9384377 [H]