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The map label for this gene is lcfB [H]

Identifier: 154247279

GI number: 154247279

Start: 3729912

End: 3731330

Strand: Direct

Name: lcfB [H]

Synonym: Xaut_3351

Alternate gene names: 154247279

Gene position: 3729912-3731330 (Clockwise)

Preceding gene: 154247278

Following gene: 154247280

Centisome position: 70.26

GC content: 72.02

Gene sequence:

>1419_bases
ATGCCCTCGCAGACCCGGCCCGGCGCGCTCTTCGACATCCTGTCAACGGCATCAGGCGAAGTCGAGGACGACAGCACCGC
CTCGGGCACGGCGGCCCTGGCATCGGCCGCCGCCGCCCTTGCCGCGCGCCTGGGGGCCGCCGGGATCCGGCCCGACGAGC
CGGTGGTGGTGAGCGTCGCCAACCGCGCGGCGGACCTTGCCGCGCTGTTCGGCGTCTGGCAGGCGGGCGGCGTGGTGGTT
CCCCTCCACGCCGCCGCCGCTGAGACGACGCGCGCGGCGATGCTGGACCAGGTCCGGCCCCGTTTCCTCCTGTCGGATGG
CGAGCTGGTCACCCTGGCCGATGTTCCGCCGCCGGAGCGGCCGCTGCTGCGGGGCGCGGCGCTGGTGGTGTTCACATCCG
GCTCTACCGGCAAGCCGAAGGGCGTCGTGCTCGGCCACGATCGCCTGGCGGCGAAGCTCGACGCGCTGCAGGAGCTCCTG
CGGCTGACGCCGCTGGACACGGTCGTCCTGCCGCTGCAGCTCATTTTCATCTTCGGCCTGTGGGTGGCGCTGCTGTCGCT
CCGGGCGGGAGCGCGGCTGGCGCTGGTGGGCAAGTTCTCGCCTGCCACGCTGCGCCGGCGGCTCCAGGGCGCGACGGTGC
TGGCCACGGTACCCTCCATGCTGCGGACCATGTTCGGCGAGGGAGAGCTGGAGGCGCCGACGCTGCGCGCCATTCTCACC
GGTGGCGAGGCGCTCGGCACGCACTTGTCCGGCCAGATCTCCCACCAGTTGCCCAGTGCCGGCGTCTTCGACCTCTATGG
CCTGACCGAGACGGGATCCTGCGACTTCTGCCTCCTGCCACGCGATGCCGCGCCCGGCCGCGGCTCCATCGGCGTGCCGA
CCCGGGGCGTCACCTACCGCCTTGCCATCGATGACGCTGCCCCCGTCACGGCACCGGCGGACGCACCACAGGGCGAGCTG
CAGGTTCGCTCGCCGTTCGGCATGCTGGGGTATCTGGACAATCCGGACCTGACCGAGGCCGCGTTCGCCGATGGCTATTT
CCGCACCGGCGACGTGGCGCGCCAACGCCCTGACGGCTTCGTTGAGATCGTCGGCCGCATCAAGGAGATCATCTCGCGCG
GTGGCAACAAGATCGCTCCGGCCGAGATCGAGCTGATCCTGTCGGCGCACCCCGCCGTGGCCGAGGTGCTCTGTGCGGGC
GTCCCGGACCCGCGGCTCGGCGAGGCCCTCCATGTCGCCGTGGTCCTCAAGCCGGGGCATGCCGCGTCCGCCGACGAGCT
TCGCAACTGGTGCAGGGAGCGGACCGAGCGCTTCAAGGTGCCTGACGCGATCCACATGGTCGATGGTCTTCCGACCGGCC
CCACCGGGAAGGCCCTGCGCGCGGGCGTTCGGGAGCTGGCCCAGGCCCTGAAGCCGTAG

Upstream 100 bases:

>100_bases
AAGATTGCCTTCTTCGAGGTCGCCCGGCGCGACGGGTTGAGGGCGGCTATCGCCTGGCGCGACGCCCGCTTCCCCGCCCG
CGACGCCTGAGGGGACCACG

Downstream 100 bases:

>100_bases
TGGCGTTGGGCTCGGCACAGGGACCTGCCTGCCGGACACGTGCCCTTCTCTCCGGCGGCGTTACGAGCCGGGCGCGGGTC
CAGGGGGCCGGTGCGATCAC

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MPSQTRPGALFDILSTASGEVEDDSTASGTAALASAAAALAARLGAAGIRPDEPVVVSVANRAADLAALFGVWQAGGVVV
PLHAAAAETTRAAMLDQVRPRFLLSDGELVTLADVPPPERPLLRGAALVVFTSGSTGKPKGVVLGHDRLAAKLDALQELL
RLTPLDTVVLPLQLIFIFGLWVALLSLRAGARLALVGKFSPATLRRRLQGATVLATVPSMLRTMFGEGELEAPTLRAILT
GGEALGTHLSGQISHQLPSAGVFDLYGLTETGSCDFCLLPRDAAPGRGSIGVPTRGVTYRLAIDDAAPVTAPADAPQGEL
QVRSPFGMLGYLDNPDLTEAAFADGYFRTGDVARQRPDGFVEIVGRIKEIISRGGNKIAPAEIELILSAHPAVAEVLCAG
VPDPRLGEALHVAVVLKPGHAASADELRNWCRERTERFKVPDAIHMVDGLPTGPTGKALRAGVRELAQALKP

Sequences:

>Translated_472_residues
MPSQTRPGALFDILSTASGEVEDDSTASGTAALASAAAALAARLGAAGIRPDEPVVVSVANRAADLAALFGVWQAGGVVV
PLHAAAAETTRAAMLDQVRPRFLLSDGELVTLADVPPPERPLLRGAALVVFTSGSTGKPKGVVLGHDRLAAKLDALQELL
RLTPLDTVVLPLQLIFIFGLWVALLSLRAGARLALVGKFSPATLRRRLQGATVLATVPSMLRTMFGEGELEAPTLRAILT
GGEALGTHLSGQISHQLPSAGVFDLYGLTETGSCDFCLLPRDAAPGRGSIGVPTRGVTYRLAIDDAAPVTAPADAPQGEL
QVRSPFGMLGYLDNPDLTEAAFADGYFRTGDVARQRPDGFVEIVGRIKEIISRGGNKIAPAEIELILSAHPAVAEVLCAG
VPDPRLGEALHVAVVLKPGHAASADELRNWCRERTERFKVPDAIHMVDGLPTGPTGKALRAGVRELAQALKP
>Mature_471_residues
PSQTRPGALFDILSTASGEVEDDSTASGTAALASAAAALAARLGAAGIRPDEPVVVSVANRAADLAALFGVWQAGGVVVP
LHAAAAETTRAAMLDQVRPRFLLSDGELVTLADVPPPERPLLRGAALVVFTSGSTGKPKGVVLGHDRLAAKLDALQELLR
LTPLDTVVLPLQLIFIFGLWVALLSLRAGARLALVGKFSPATLRRRLQGATVLATVPSMLRTMFGEGELEAPTLRAILTG
GEALGTHLSGQISHQLPSAGVFDLYGLTETGSCDFCLLPRDAAPGRGSIGVPTRGVTYRLAIDDAAPVTAPADAPQGELQ
VRSPFGMLGYLDNPDLTEAAFADGYFRTGDVARQRPDGFVEIVGRIKEIISRGGNKIAPAEIELILSAHPAVAEVLCAGV
PDPRLGEALHVAVVLKPGHAASADELRNWCRERTERFKVPDAIHMVDGLPTGPTGKALRAGVRELAQALKP

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI187761345, Length=473, Percent_Identity=27.4841437632135, Blast_Score=146, Evalue=5e-35,
Organism=Homo sapiens, GI187761343, Length=473, Percent_Identity=27.4841437632135, Blast_Score=146, Evalue=5e-35,
Organism=Homo sapiens, GI156151445, Length=353, Percent_Identity=31.4447592067989, Blast_Score=145, Evalue=6e-35,
Organism=Homo sapiens, GI38505220, Length=354, Percent_Identity=27.683615819209, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI157311624, Length=359, Percent_Identity=27.8551532033426, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI157311622, Length=359, Percent_Identity=27.8551532033426, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI58082049, Length=361, Percent_Identity=28.2548476454294, Blast_Score=109, Evalue=7e-24,
Organism=Homo sapiens, GI42544132, Length=353, Percent_Identity=24.6458923512748, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI115511026, Length=434, Percent_Identity=24.4239631336406, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI122937307, Length=266, Percent_Identity=29.6992481203008, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI8923896, Length=365, Percent_Identity=27.1232876712329, Blast_Score=87, Evalue=5e-17,
Organism=Homo sapiens, GI253970489, Length=367, Percent_Identity=28.3378746594005, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI28416953, Length=372, Percent_Identity=26.0752688172043, Blast_Score=79, Evalue=9e-15,
Organism=Homo sapiens, GI13236579, Length=371, Percent_Identity=23.7196765498652, Blast_Score=66, Evalue=8e-11,
Organism=Escherichia coli, GI145693145, Length=353, Percent_Identity=30.5949008498584, Blast_Score=131, Evalue=8e-32,
Organism=Escherichia coli, GI1788107, Length=351, Percent_Identity=30.1994301994302, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI1786801, Length=400, Percent_Identity=29.25, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI221142682, Length=472, Percent_Identity=25.8474576271186, Blast_Score=112, Evalue=7e-26,
Organism=Escherichia coli, GI1790505, Length=367, Percent_Identity=27.7929155313352, Blast_Score=111, Evalue=8e-26,
Organism=Escherichia coli, GI1788595, Length=453, Percent_Identity=27.1523178807947, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1786810, Length=250, Percent_Identity=30.8, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1789201, Length=367, Percent_Identity=28.3378746594005, Blast_Score=87, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17557194, Length=360, Percent_Identity=29.1666666666667, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17538037, Length=391, Percent_Identity=28.9002557544757, Blast_Score=138, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI17558820, Length=364, Percent_Identity=27.1978021978022, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI32563687, Length=446, Percent_Identity=23.9910313901345, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17531443, Length=514, Percent_Identity=24.5136186770428, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17559526, Length=395, Percent_Identity=25.3164556962025, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17560140, Length=351, Percent_Identity=28.2051282051282, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71994690, Length=355, Percent_Identity=27.6056338028169, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71994694, Length=355, Percent_Identity=27.6056338028169, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71985884, Length=351, Percent_Identity=27.0655270655271, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71994703, Length=355, Percent_Identity=27.6056338028169, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17560308, Length=359, Percent_Identity=26.7409470752089, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI71996755, Length=325, Percent_Identity=27.6923076923077, Blast_Score=89, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI71982997, Length=398, Percent_Identity=27.1356783919598, Blast_Score=89, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI71983001, Length=398, Percent_Identity=27.1356783919598, Blast_Score=89, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6319699, Length=371, Percent_Identity=30.7277628032345, Blast_Score=162, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6323182, Length=376, Percent_Identity=26.5957446808511, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6323903, Length=424, Percent_Identity=27.122641509434, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6319264, Length=375, Percent_Identity=25.6, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI18859661, Length=374, Percent_Identity=32.0855614973262, Blast_Score=161, Evalue=8e-40,
Organism=Drosophila melanogaster, GI24581924, Length=369, Percent_Identity=30.0813008130081, Blast_Score=135, Evalue=8e-32,
Organism=Drosophila melanogaster, GI21355181, Length=378, Percent_Identity=27.2486772486773, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24667959, Length=373, Percent_Identity=28.1501340482574, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24667955, Length=373, Percent_Identity=28.1501340482574, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI62472339, Length=373, Percent_Identity=28.1501340482574, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21356947, Length=407, Percent_Identity=23.5872235872236, Blast_Score=89, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24653035, Length=369, Percent_Identity=24.6612466124661, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24648253, Length=357, Percent_Identity=26.0504201680672, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24648255, Length=346, Percent_Identity=26.3005780346821, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI281365686, Length=428, Percent_Identity=21.9626168224299, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI20130357, Length=361, Percent_Identity=24.6537396121884, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI21356441, Length=482, Percent_Identity=23.4439834024896, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI161076582, Length=375, Percent_Identity=24.2666666666667, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21358303, Length=351, Percent_Identity=23.6467236467236, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24648257, Length=512, Percent_Identity=24.0234375, Blast_Score=77, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 49247; Mature: 49116

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSQTRPGALFDILSTASGEVEDDSTASGTAALASAAAALAARLGAAGIRPDEPVVVSVA
CCCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEC
NRAADLAALFGVWQAGGVVVPLHAAAAETTRAAMLDQVRPRFLLSDGELVTLADVPPPER
HHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHCCCCEEECCCCEEEEECCCCCCC
PLLRGAALVVFTSGSTGKPKGVVLGHDRLAAKLDALQELLRLTPLDTVVLPLQLIFIFGL
HHHCCEEEEEEECCCCCCCCCEEEECHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
WVALLSLRAGARLALVGKFSPATLRRRLQGATVLATVPSMLRTMFGEGELEAPTLRAILT
HHHHHHHCCCCEEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHC
GGEALGTHLSGQISHQLPSAGVFDLYGLTETGSCDFCLLPRDAAPGRGSIGVPTRGVTYR
CCHHHHCCCCCHHHHCCCCCCCEEEECCCCCCCCCEEEECCCCCCCCCCCCCCCCCEEEE
LAIDDAAPVTAPADAPQGELQVRSPFGMLGYLDNPDLTEAAFADGYFRTGDVARQRPDGF
EEECCCCCCCCCCCCCCCCEEEECCCCEEEECCCCCCHHHHHHCCCCCCCCHHHCCCCHH
VEIVGRIKEIISRGGNKIAPAEIELILSAHPAVAEVLCAGVPDPRLGEALHVAVVLKPGH
HHHHHHHHHHHHCCCCEECCCEEEEEEECCHHHHHHHHCCCCCCCCCCEEEEEEEEECCC
AASADELRNWCRERTERFKVPDAIHMVDGLPTGPTGKALRAGVRELAQALKP
CCCHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PSQTRPGALFDILSTASGEVEDDSTASGTAALASAAAALAARLGAAGIRPDEPVVVSVA
CCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEC
NRAADLAALFGVWQAGGVVVPLHAAAAETTRAAMLDQVRPRFLLSDGELVTLADVPPPER
HHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHCCCCEEECCCCEEEEECCCCCCC
PLLRGAALVVFTSGSTGKPKGVVLGHDRLAAKLDALQELLRLTPLDTVVLPLQLIFIFGL
HHHCCEEEEEEECCCCCCCCCEEEECHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
WVALLSLRAGARLALVGKFSPATLRRRLQGATVLATVPSMLRTMFGEGELEAPTLRAILT
HHHHHHHCCCCEEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHC
GGEALGTHLSGQISHQLPSAGVFDLYGLTETGSCDFCLLPRDAAPGRGSIGVPTRGVTYR
CCHHHHCCCCCHHHHCCCCCCCEEEECCCCCCCCCEEEECCCCCCCCCCCCCCCCCEEEE
LAIDDAAPVTAPADAPQGELQVRSPFGMLGYLDNPDLTEAAFADGYFRTGDVARQRPDGF
EEECCCCCCCCCCCCCCCCEEEECCCCEEEECCCCCCHHHHHHCCCCCCCCHHHCCCCHH
VEIVGRIKEIISRGGNKIAPAEIELILSAHPAVAEVLCAGVPDPRLGEALHVAVVLKPGH
HHHHHHHHHHHHCCCCEECCCEEEEEEECCHHHHHHHHCCCCCCCCCCEEEEEEEEECCC
AASADELRNWCRERTERFKVPDAIHMVDGLPTGPTGKALRAGVRELAQALKP
CCCHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]