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The map label for this gene is menB [C]

Identifier: 154247278

GI number: 154247278

Start: 3729080

End: 3729901

Strand: Direct

Name: menB [C]

Synonym: Xaut_3350

Alternate gene names: 154247278

Gene position: 3729080-3729901 (Clockwise)

Preceding gene: 154247277

Following gene: 154247279

Centisome position: 70.24

GC content: 69.1

Gene sequence:

>822_bases
ATGACCTATTCCAAGATCCTCTACGAGACGCTCCATGGCGTCGCCCGCATCACCCTCAACCGGCCCGAGCGCACCAATGC
CCTCGACCAGGAGATGCTGGGCGAGATCAATGCCGCCATGGACGCGGCCGAGGCCGATGCGGGCGTGAAGGCGGTGATCG
TGCGTGGAGCCGGCAACGCCTTCTCCTCCGGCTTCGACCTCAAGGCGCAGATGGAGGCGCGCCCGGCGGGCGTGGATGCC
TGGCGCCCGCTGCTGCGCAAGGATTTCGACACGGTGATGCGGTTCTGGCATTGCCCCAAGCCCACCATCGCCGCGGTGCG
CGGCCCGTGCCTCGCCGGTGCCTGTGAGCTGGCCCTCGCCTGCGACATGACCATCGCCACCGAGGATGCCTTCTTCGGCG
AGCCGGAACTGAAGTTCGGCGCCGGCATCGTGGTGATGCTGCTGCCCTGGATCGTCGGTCCCAAGATCGCCAAGGAGATC
ATCCTCCTGGGCGAGGACCGGGTGCCGGCCCGGCGCGCCGCCGAGATCGGCATGGTCAACCGCGTCGTCGATGGCGACGG
GCTGGACGCCGAGGCCCTGCGCATCGCCCGGCACATCGGGGCCATCGATCCGGGTCTGGTGAAGGAGACCAAGCGCGCGC
TCAACCGGGCACTGGAGACGCAGGGTATGCTGCAGGCGCTGGAAAGCGCGCTGGAGATCGATCTTGCCATCGAGGGGGCC
GGCTCGCCCGACAAGATTGCCTTCTTCGAGGTCGCCCGGCGCGACGGGTTGAGGGCGGCTATCGCCTGGCGCGACGCCCG
CTTCCCCGCCCGCGACGCCTGA

Upstream 100 bases:

>100_bases
GCCCCTTCCTCGGCGTCTTCGCCATGCTCCGCCTGAGGCTGCGGCCCGAGGCTGCGGCCATGGCGGGCGGCCGCCGCTGA
CGTTTCCCGACGAGGCACCC

Downstream 100 bases:

>100_bases
GGGGACCACGATGCCCTCGCAGACCCGGCCCGGCGCGCTCTTCGACATCCTGTCAACGGCATCAGGCGAAGTCGAGGACG
ACAGCACCGCCTCGGGCACG

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MTYSKILYETLHGVARITLNRPERTNALDQEMLGEINAAMDAAEADAGVKAVIVRGAGNAFSSGFDLKAQMEARPAGVDA
WRPLLRKDFDTVMRFWHCPKPTIAAVRGPCLAGACELALACDMTIATEDAFFGEPELKFGAGIVVMLLPWIVGPKIAKEI
ILLGEDRVPARRAAEIGMVNRVVDGDGLDAEALRIARHIGAIDPGLVKETKRALNRALETQGMLQALESALEIDLAIEGA
GSPDKIAFFEVARRDGLRAAIAWRDARFPARDA

Sequences:

>Translated_273_residues
MTYSKILYETLHGVARITLNRPERTNALDQEMLGEINAAMDAAEADAGVKAVIVRGAGNAFSSGFDLKAQMEARPAGVDA
WRPLLRKDFDTVMRFWHCPKPTIAAVRGPCLAGACELALACDMTIATEDAFFGEPELKFGAGIVVMLLPWIVGPKIAKEI
ILLGEDRVPARRAAEIGMVNRVVDGDGLDAEALRIARHIGAIDPGLVKETKRALNRALETQGMLQALESALEIDLAIEGA
GSPDKIAFFEVARRDGLRAAIAWRDARFPARDA
>Mature_272_residues
TYSKILYETLHGVARITLNRPERTNALDQEMLGEINAAMDAAEADAGVKAVIVRGAGNAFSSGFDLKAQMEARPAGVDAW
RPLLRKDFDTVMRFWHCPKPTIAAVRGPCLAGACELALACDMTIATEDAFFGEPELKFGAGIVVMLLPWIVGPKIAKEII
LLGEDRVPARRAAEIGMVNRVVDGDGLDAEALRIARHIGAIDPGLVKETKRALNRALETQGMLQALESALEIDLAIEGAG
SPDKIAFFEVARRDGLRAAIAWRDARFPARDA

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=261, Percent_Identity=31.8007662835249, Blast_Score=105, Evalue=4e-23,
Organism=Homo sapiens, GI70995211, Length=225, Percent_Identity=30.6666666666667, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI31542718, Length=262, Percent_Identity=27.0992366412214, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI260274832, Length=220, Percent_Identity=28.6363636363636, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI45643119, Length=220, Percent_Identity=28.6363636363636, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI260275230, Length=220, Percent_Identity=28.6363636363636, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI68989263, Length=217, Percent_Identity=30.8755760368664, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI221136756, Length=246, Percent_Identity=26.4227642276423, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI221136753, Length=246, Percent_Identity=26.4227642276423, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI221307494, Length=246, Percent_Identity=26.4227642276423, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI20127408, Length=208, Percent_Identity=28.3653846153846, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI4502327, Length=274, Percent_Identity=29.9270072992701, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1788597, Length=259, Percent_Identity=32.046332046332, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787659, Length=221, Percent_Identity=32.1266968325792, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI221142681, Length=253, Percent_Identity=32.4110671936759, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1787660, Length=277, Percent_Identity=31.4079422382672, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1790281, Length=181, Percent_Identity=32.5966850828729, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI87082183, Length=227, Percent_Identity=26.8722466960352, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1788682, Length=204, Percent_Identity=28.4313725490196, Blast_Score=64, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI25145438, Length=220, Percent_Identity=37.2727272727273, Blast_Score=117, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI17554946, Length=217, Percent_Identity=31.7972350230415, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17534483, Length=218, Percent_Identity=31.651376146789, Blast_Score=91, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17540714, Length=228, Percent_Identity=33.3333333333333, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17558304, Length=187, Percent_Identity=32.620320855615, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17536985, Length=222, Percent_Identity=26.5765765765766, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17535521, Length=222, Percent_Identity=26.5765765765766, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI25144276, Length=204, Percent_Identity=30.3921568627451, Blast_Score=70, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17508951, Length=204, Percent_Identity=30.3921568627451, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17508953, Length=204, Percent_Identity=30.3921568627451, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17560910, Length=193, Percent_Identity=26.9430051813472, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17540306, Length=196, Percent_Identity=28.5714285714286, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI20129971, Length=263, Percent_Identity=30.7984790874525, Blast_Score=106, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24653477, Length=263, Percent_Identity=30.7984790874525, Blast_Score=106, Evalue=1e-23,
Organism=Drosophila melanogaster, GI19920382, Length=221, Percent_Identity=30.316742081448, Blast_Score=95, Evalue=4e-20,
Organism=Drosophila melanogaster, GI21357171, Length=233, Percent_Identity=27.8969957081545, Blast_Score=84, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24653139, Length=231, Percent_Identity=30.7359307359307, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24654903, Length=192, Percent_Identity=27.0833333333333, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24650670, Length=181, Percent_Identity=31.4917127071823, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI19922422, Length=201, Percent_Identity=26.865671641791, Blast_Score=68, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 29503; Mature: 29372

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYSKILYETLHGVARITLNRPERTNALDQEMLGEINAAMDAAEADAGVKAVIVRGAGNA
CCHHHHHHHHHHHHHEEEECCCHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCC
FSSGFDLKAQMEARPAGVDAWRPLLRKDFDTVMRFWHCPKPTIAAVRGPCLAGACELALA
CCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHH
CDMTIATEDAFFGEPELKFGAGIVVMLLPWIVGPKIAKEIILLGEDRVPARRAAEIGMVN
CCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHEECCCCCCHHHHHHHHHHH
RVVDGDGLDAEALRIARHIGAIDPGLVKETKRALNRALETQGMLQALESALEIDLAIEGA
HEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECC
GSPDKIAFFEVARRDGLRAAIAWRDARFPARDA
CCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCC
>Mature Secondary Structure 
TYSKILYETLHGVARITLNRPERTNALDQEMLGEINAAMDAAEADAGVKAVIVRGAGNA
CHHHHHHHHHHHHHEEEECCCHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCC
FSSGFDLKAQMEARPAGVDAWRPLLRKDFDTVMRFWHCPKPTIAAVRGPCLAGACELALA
CCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHH
CDMTIATEDAFFGEPELKFGAGIVVMLLPWIVGPKIAKEIILLGEDRVPARRAAEIGMVN
CCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHEECCCCCCHHHHHHHHHHH
RVVDGDGLDAEALRIARHIGAIDPGLVKETKRALNRALETQGMLQALESALEIDLAIEGA
HEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECC
GSPDKIAFFEVARRDGLRAAIAWRDARFPARDA
CCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA