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The map label for this gene is glyS [H]

Identifier: 154247257

GI number: 154247257

Start: 3702154

End: 3704355

Strand: Reverse

Name: glyS [H]

Synonym: Xaut_3329

Alternate gene names: 154247257

Gene position: 3704355-3702154 (Counterclockwise)

Preceding gene: 154247258

Following gene: 154247250

Centisome position: 69.78

GC content: 67.94

Gene sequence:

>2202_bases
ATGCCCGACCTTCTCATTGAACTCTTCTCCGAGGAAATCCCCGCCCGCATGCAGGCCCAGGCGGCCGACGGGCTGAAGAA
GCTCGTCACCGACGCGCTGGTGGAGCGGGGCCTGCTCTATGAGGGCGCGAAGGCCTTCGTGACCCCGCGCCGGCTGGCGC
TGGCGGTGCACGGCCTGCCCGGCCGGCAGGCGGACCAGCGCGAGGAGAAGAAGGGTCCGCGCGTGGGCGCGCCGGACGGC
GCCATCGCCGGCTTCCTGAAATCGGCGGGGCTTTCGTCCCTCGATCAGGCCACCGTGCAGAGCGACCCCAAGAAGGGCGA
CTTCTATGTCGCCGTGGTGGAAAAGCCCGGCCGCCCCACCCCCGAGGTGATCGCCGAGATCATCCCCGCCATCATCCGCA
GCTTCCCCTGGCCGAAATCCATGCGCTGGGGCTCGGGAAGCCTGCGCTGGGTGCGGCCGCTGCATTCCATCGTCGCCACC
TTCGGGGCGGAAGGGGACGAGCCGGAAGTGGTGCGCTTCGAGGTGGACGGCATCGTCTCCTCCAACATCACCTACGGCCA
CCGCTTCCTTGCGCCCGCACCCATCACGGTGAAGCGGCTGGACGACTGGATGGCCAAGCTCGAGGCCGCCAAAGTGGTGG
TCGACCGCGACCGCCGCAAGGACATCATCCTTGCCGATGCCAAGAACGAGGCGTTCGCCAACAGGCTTGAGCTGGTGGAG
GACGAGGGTCTGCTGGACGAGGTGGCGGGGCTGGTGGAATGGCCCGTGGTGCTCATGGGCGCGTTCGAGGAGCGTTTCCT
CGATATCCCGGATGAAGTCATCCGCGCCACCATCCGCGCCAACCAGAAGTGCTTCGTGCTGAAGGACCCGGCCACCGGGC
GCCTCGCCAACCGCTTCCTGCTGATCTCCAATCTGGTGGCGAGCGACGGCGGCGCGGCCATCGTCGCCGGCAACGAGCGC
GTCATCCGCGCCCGCCTGTCGGACGCGAAATTCTTCTGGGATACGGACCGGGTGAACGGGCTGGAAAGCCGGATGCCCCG
GTTCCAGTCCATCACCTTCCACGAGAAGCTGGGCTCGCAGGCCGAGCGCATCGAGCGCATCCGCCGCCTCGCGGTGGAGA
TCGCGCCGAAGGTGGGCGCGCCCGCCGCGCTGGCCGAGGACGCGGCGCTGTTCTGCAAGGCGGACCTGCTCACCGAGGTG
GTGGGCGAGTTCCCCGAGCTTCAGGGCCTCATGGGCCACTATTATGCGCAGGGCGAGGGGCTGGCTCCGGAAGTCGCCGC
CGCCTGCGAGGAGCATTACAAGCCGCAAGGCCCCGGCGACCGTGTGCCCACCGCGCCGGTCTCCGTCGCCGTGGCGCTGG
CAGACAAGATCGACACTTTGGTCGGCTTCTGGGCGATTGATGAGAAGCCCACCGGGAGCAAGGACCCCTACGCGCTGCGC
CGGGCAGCGCTTGGGGTGATCCGGATCGTGCTGGAGAATGGGCTGCGGTTGCGGTTGGCGGATGTGTTCACAGGGCACTT
CAAGCTAGCGGACCAAGACATCATCCGCACCGCCAACGAGGCTCGCATCAAGCGCGTAGAGGACGCGCTCGGAGATCAGG
GCGGCGCGGTCGTGGGCAATGATGTCGCGATGTGGGTCAGCAGAAAGTTTGGCGACAGGACTGCCGACTCCCGCAGCATC
GACCTCCTCTCCTTCTTTGCCGATCGCCTGAAAGTCCATCTGCGCGAGCATGGCGCGCGCCATGACCTCGTGGATGCGGT
CTTTGCACTGGAGGGGCAGGACGACCTGTTCCTGCTGGTGGAGCGCATGACGGCGCTGGCCCGCTTCCTCGACACCGAGG
ACGGCAGGACACTGCTCGCCGGCTATCGCCGCGCGGTGAACATCCTGCGCATCGAGGAGAAGAAGGACGGCCGCGCCTTC
GCCGGCCCCACCGATGCCACCCTGCTGAAGGAGCCGGCCGAAGAGCGCCTCGCCGATGCCATCGAGCAGGTGAAGGGCCG
CGTCGCCGCCGCCGTGGCGGCGGAGGACTTCGAGACCGCCATGACCGCGCTGGCGGACCTGCGCGGTCCCGTGGATGCCT
TCTTCGACGCGGTCACGGTGAATGATCAAGACCCCGCCCTGCGCGAGAACCGCCTGAAGCTGCTGGATGAAATCCGCGCC
GCCACCCGCGGCATCGCCGATTTCGACCGCATCGGCGGCTGA

Upstream 100 bases:

>100_bases
TGGCTCAAGACGGCGGCCGGCGGGGCGGCCTGAGCGCCCCTCCCCCGCTTCCGGCCCCCTTCATCGCCTTGCGCGACCCA
GATTGATCAAGACTGATCCC

Downstream 100 bases:

>100_bases
CGCTCCGCCCCCGCGCCCCGGCCCGAGCCGCTCAACAAAAAGCCCCGGAGCAGCGCTCCGGGGCTTTTCTCATCAGCGAT
GACAGACGGCTCAGCCGCGG

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 733; Mature: 732

Protein sequence:

>733_residues
MPDLLIELFSEEIPARMQAQAADGLKKLVTDALVERGLLYEGAKAFVTPRRLALAVHGLPGRQADQREEKKGPRVGAPDG
AIAGFLKSAGLSSLDQATVQSDPKKGDFYVAVVEKPGRPTPEVIAEIIPAIIRSFPWPKSMRWGSGSLRWVRPLHSIVAT
FGAEGDEPEVVRFEVDGIVSSNITYGHRFLAPAPITVKRLDDWMAKLEAAKVVVDRDRRKDIILADAKNEAFANRLELVE
DEGLLDEVAGLVEWPVVLMGAFEERFLDIPDEVIRATIRANQKCFVLKDPATGRLANRFLLISNLVASDGGAAIVAGNER
VIRARLSDAKFFWDTDRVNGLESRMPRFQSITFHEKLGSQAERIERIRRLAVEIAPKVGAPAALAEDAALFCKADLLTEV
VGEFPELQGLMGHYYAQGEGLAPEVAAACEEHYKPQGPGDRVPTAPVSVAVALADKIDTLVGFWAIDEKPTGSKDPYALR
RAALGVIRIVLENGLRLRLADVFTGHFKLADQDIIRTANEARIKRVEDALGDQGGAVVGNDVAMWVSRKFGDRTADSRSI
DLLSFFADRLKVHLREHGARHDLVDAVFALEGQDDLFLLVERMTALARFLDTEDGRTLLAGYRRAVNILRIEEKKDGRAF
AGPTDATLLKEPAEERLADAIEQVKGRVAAAVAAEDFETAMTALADLRGPVDAFFDAVTVNDQDPALRENRLKLLDEIRA
ATRGIADFDRIGG

Sequences:

>Translated_733_residues
MPDLLIELFSEEIPARMQAQAADGLKKLVTDALVERGLLYEGAKAFVTPRRLALAVHGLPGRQADQREEKKGPRVGAPDG
AIAGFLKSAGLSSLDQATVQSDPKKGDFYVAVVEKPGRPTPEVIAEIIPAIIRSFPWPKSMRWGSGSLRWVRPLHSIVAT
FGAEGDEPEVVRFEVDGIVSSNITYGHRFLAPAPITVKRLDDWMAKLEAAKVVVDRDRRKDIILADAKNEAFANRLELVE
DEGLLDEVAGLVEWPVVLMGAFEERFLDIPDEVIRATIRANQKCFVLKDPATGRLANRFLLISNLVASDGGAAIVAGNER
VIRARLSDAKFFWDTDRVNGLESRMPRFQSITFHEKLGSQAERIERIRRLAVEIAPKVGAPAALAEDAALFCKADLLTEV
VGEFPELQGLMGHYYAQGEGLAPEVAAACEEHYKPQGPGDRVPTAPVSVAVALADKIDTLVGFWAIDEKPTGSKDPYALR
RAALGVIRIVLENGLRLRLADVFTGHFKLADQDIIRTANEARIKRVEDALGDQGGAVVGNDVAMWVSRKFGDRTADSRSI
DLLSFFADRLKVHLREHGARHDLVDAVFALEGQDDLFLLVERMTALARFLDTEDGRTLLAGYRRAVNILRIEEKKDGRAF
AGPTDATLLKEPAEERLADAIEQVKGRVAAAVAAEDFETAMTALADLRGPVDAFFDAVTVNDQDPALRENRLKLLDEIRA
ATRGIADFDRIGG
>Mature_732_residues
PDLLIELFSEEIPARMQAQAADGLKKLVTDALVERGLLYEGAKAFVTPRRLALAVHGLPGRQADQREEKKGPRVGAPDGA
IAGFLKSAGLSSLDQATVQSDPKKGDFYVAVVEKPGRPTPEVIAEIIPAIIRSFPWPKSMRWGSGSLRWVRPLHSIVATF
GAEGDEPEVVRFEVDGIVSSNITYGHRFLAPAPITVKRLDDWMAKLEAAKVVVDRDRRKDIILADAKNEAFANRLELVED
EGLLDEVAGLVEWPVVLMGAFEERFLDIPDEVIRATIRANQKCFVLKDPATGRLANRFLLISNLVASDGGAAIVAGNERV
IRARLSDAKFFWDTDRVNGLESRMPRFQSITFHEKLGSQAERIERIRRLAVEIAPKVGAPAALAEDAALFCKADLLTEVV
GEFPELQGLMGHYYAQGEGLAPEVAAACEEHYKPQGPGDRVPTAPVSVAVALADKIDTLVGFWAIDEKPTGSKDPYALRR
AALGVIRIVLENGLRLRLADVFTGHFKLADQDIIRTANEARIKRVEDALGDQGGAVVGNDVAMWVSRKFGDRTADSRSID
LLSFFADRLKVHLREHGARHDLVDAVFALEGQDDLFLLVERMTALARFLDTEDGRTLLAGYRRAVNILRIEEKKDGRAFA
GPTDATLLKEPAEERLADAIEQVKGRVAAAVAAEDFETAMTALADLRGPVDAFFDAVTVNDQDPALRENRLKLLDEIRAA
TRGIADFDRIGG

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=734, Percent_Identity=33.6512261580381, Blast_Score=369, Evalue=1e-103,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 80299; Mature: 80167

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDLLIELFSEEIPARMQAQAADGLKKLVTDALVERGLLYEGAKAFVTPRRLALAVHGLP
CCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHCCCHHHCCHHHHEEEEECCC
GRQADQREEKKGPRVGAPDGAIAGFLKSAGLSSLDQATVQSDPKKGDFYVAVVEKPGRPT
CCCCCHHHHHCCCCCCCCCHHHHHHHHHCCCCHHHHHHHCCCCCCCCEEEEEECCCCCCC
PEVIAEIIPAIIRSFPWPKSMRWGSGSLRWVRPLHSIVATFGAEGDEPEVVRFEVDGIVS
HHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCEEE
SNITYGHRFLAPAPITVKRLDDWMAKLEAAKVVVDRDRRKDIILADAKNEAFANRLELVE
CCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHC
DEGLLDEVAGLVEWPVVLMGAFEERFLDIPDEVIRATIRANQKCFVLKDPATGRLANRFL
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHH
LISNLVASDGGAAIVAGNERVIRARLSDAKFFWDTDRVNGLESRMPRFQSITFHEKLGSQ
HHHHHHHCCCCEEEEECCCEEEEEECCCCCEEECCHHCCCHHHHCCHHHCCHHHHHHCHH
AERIERIRRLAVEIAPKVGAPAALAEDAALFCKADLLTEVVGEFPELQGLMGHYYAQGEG
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCC
LAPEVAAACEEHYKPQGPGDRVPTAPVSVAVALADKIDTLVGFWAIDEKPTGSKDPYALR
CCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCCCCCHHHHH
RAALGVIRIVLENGLRLRLADVFTGHFKLADQDIIRTANEARIKRVEDALGDQGGAVVGN
HHHHHHHHHHHHCCCEEEEHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHCCCCCEEECC
DVAMWVSRKFGDRTADSRSIDLLSFFADRLKVHLREHGARHDLVDAVFALEGQDDLFLLV
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHH
ERMTALARFLDTEDGRTLLAGYRRAVNILRIEEKKDGRAFAGPTDATLLKEPAEERLADA
HHHHHHHHHHCCCCCHHHHHHHHHHHHHEEEECCCCCCEECCCCCHHHHHCCHHHHHHHH
IEQVKGRVAAAVAAEDFETAMTALADLRGPVDAFFDAVTVNDQDPALRENRLKLLDEIRA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCEEEECCCCCHHHHHHHHHHHHHHH
ATRGIADFDRIGG
HHCCCHHHHHCCC
>Mature Secondary Structure 
PDLLIELFSEEIPARMQAQAADGLKKLVTDALVERGLLYEGAKAFVTPRRLALAVHGLP
CHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHCCCHHHCCHHHHEEEEECCC
GRQADQREEKKGPRVGAPDGAIAGFLKSAGLSSLDQATVQSDPKKGDFYVAVVEKPGRPT
CCCCCHHHHHCCCCCCCCCHHHHHHHHHCCCCHHHHHHHCCCCCCCCEEEEEECCCCCCC
PEVIAEIIPAIIRSFPWPKSMRWGSGSLRWVRPLHSIVATFGAEGDEPEVVRFEVDGIVS
HHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCEEE
SNITYGHRFLAPAPITVKRLDDWMAKLEAAKVVVDRDRRKDIILADAKNEAFANRLELVE
CCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHC
DEGLLDEVAGLVEWPVVLMGAFEERFLDIPDEVIRATIRANQKCFVLKDPATGRLANRFL
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHH
LISNLVASDGGAAIVAGNERVIRARLSDAKFFWDTDRVNGLESRMPRFQSITFHEKLGSQ
HHHHHHHCCCCEEEEECCCEEEEEECCCCCEEECCHHCCCHHHHCCHHHCCHHHHHHCHH
AERIERIRRLAVEIAPKVGAPAALAEDAALFCKADLLTEVVGEFPELQGLMGHYYAQGEG
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCC
LAPEVAAACEEHYKPQGPGDRVPTAPVSVAVALADKIDTLVGFWAIDEKPTGSKDPYALR
CCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCCCCCHHHHH
RAALGVIRIVLENGLRLRLADVFTGHFKLADQDIIRTANEARIKRVEDALGDQGGAVVGN
HHHHHHHHHHHHCCCEEEEHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHCCCCCEEECC
DVAMWVSRKFGDRTADSRSIDLLSFFADRLKVHLREHGARHDLVDAVFALEGQDDLFLLV
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHH
ERMTALARFLDTEDGRTLLAGYRRAVNILRIEEKKDGRAFAGPTDATLLKEPAEERLADA
HHHHHHHHHHCCCCCHHHHHHHHHHHHHEEEECCCCCCEECCCCCHHHHHCCHHHHHHHH
IEQVKGRVAAAVAAEDFETAMTALADLRGPVDAFFDAVTVNDQDPALRENRLKLLDEIRA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCEEEECCCCCHHHHHHHHHHHHHHH
ATRGIADFDRIGG
HHCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]