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The map label for this gene is cspA [C]

Identifier: 154247142

GI number: 154247142

Start: 3561830

End: 3562036

Strand: Direct

Name: cspA [C]

Synonym: Xaut_3214

Alternate gene names: 154247142

Gene position: 3561830-3562036 (Clockwise)

Preceding gene: 154247141

Following gene: 154247145

Centisome position: 67.09

GC content: 59.42

Gene sequence:

>207_bases
ATGGCCACAGGTACCGTCAAGTTTTTCAATTCCCAGAAGGGTTTCGGCTTCATCCAGCAGGATGAGGGTGGCCCCGACGT
CTTCGTGCACATCTCGGACGTGGAGCGCTCCGGCCTCTATGGCCTGAACGATGGCCAGAAGGTGTCCTTCGACATCGTCG
CGGACAACCGCGGTAAGAGCAAGGCGTCGAACCTCGCCGCTCTCTGA

Upstream 100 bases:

>100_bases
TCGTCTAGAACTACGCCCCGCTGCGCCGCGACGTTGGTCGTGCGCGGGGGTGACTGGCGCAGGCACAGCGATAAGCGACC
TGCGCGCACAGGAGGCCACC

Downstream 100 bases:

>100_bases
GCTGACGCTCTGAATTAAGAAACCCCGGTCACCGCAAGGTGGCCGGGGTTTTTCGCGTTTGGAGCCGGGCTCGATCGATC
GCCGTGCTCAGCGCAGCACG

Product: cold-shock DNA-binding domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 68; Mature: 67

Protein sequence:

>68_residues
MATGTVKFFNSQKGFGFIQQDEGGPDVFVHISDVERSGLYGLNDGQKVSFDIVADNRGKSKASNLAAL

Sequences:

>Translated_68_residues
MATGTVKFFNSQKGFGFIQQDEGGPDVFVHISDVERSGLYGLNDGQKVSFDIVADNRGKSKASNLAAL
>Mature_67_residues
ATGTVKFFNSQKGFGFIQQDEGGPDVFVHISDVERSGLYGLNDGQKVSFDIVADNRGKSKASNLAAL

Specific function: Binds To And Stimulates The Transcription Of The CCAAT- Containing, Cold-Shock-Inducible Promoters Of The H-Ns And Gyra Proteins. Binds Also To The Inverted Repeat 5'-ATTGG-3'. [C]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1789979, Length=66, Percent_Identity=50, Blast_Score=64, Evalue=1e-12,
Organism=Escherichia coli, GI1786841, Length=65, Percent_Identity=47.6923076923077, Blast_Score=62, Evalue=8e-12,
Organism=Escherichia coli, GI2367114, Length=66, Percent_Identity=46.969696969697, Blast_Score=61, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 7281; Mature: 7150

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATGTVKFFNSQKGFGFIQQDEGGPDVFVHISDVERSGLYGLNDGQKVSFDIVADNRGKS
CCCCEEEEEECCCCCEEEEECCCCCEEEEEEECCCCCCCEECCCCCEEEEEEEECCCCCC
KASNLAAL
CCCCCCCC
>Mature Secondary Structure 
ATGTVKFFNSQKGFGFIQQDEGGPDVFVHISDVERSGLYGLNDGQKVSFDIVADNRGKS
CCCEEEEEECCCCCEEEEECCCCCEEEEEEECCCCCCCEECCCCCEEEEEEEECCCCCC
KASNLAAL
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]