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The map label for this gene is yjbJ [H]

Identifier: 154247085

GI number: 154247085

Start: 3504095

End: 3505060

Strand: Reverse

Name: yjbJ [H]

Synonym: Xaut_3156

Alternate gene names: 154247085

Gene position: 3505060-3504095 (Counterclockwise)

Preceding gene: 154247086

Following gene: 154247077

Centisome position: 66.02

GC content: 73.71

Gene sequence:

>966_bases
ATGGCACCGGCCGCATCGCCCCGTTTTAGGCGCGCCGGCCGGATCATCGCTGCTGCCGTGCTCGCCCTTCTCGGCGCCGG
GCCGGCCTTCGGCCAGAGCACGGGCGAAGCCGCCCCGGAGAAGCCGGCCGCAGCATCGCCCACCCCGCCCGATCCCACGC
CCCGCGCGGGGGGCGAAACGGTGGACCAGGCCATCTGCCGCCTCATCGACGCGGCGGCGGCCAAAGAGGGCCTGCCGGCC
GCCTTTCTCACCCGCCTCATCTGGCGCGAAAGCTCGTTCCGGCCCCACGTGGTGAGCAACAAGGGGGCGCAGGGCATCGC
CCAGTTCATGCCCGGCACGGCGAAGGAGCGCGGGCTTCAAAATCCGTTCGATCCGGAGGCGGCGATCCCGGCCTCAGCGG
CGCTGCTCGCCGACCTCAACCGCCGCTTCGGCAATCTGGGGCTGGCGGCGGCGGCCTACAATGCCGGTCCCGGGCGCGTC
TCCGGCTTCCTCAACGGCGGCGGACTGCCGGAAGAGACGCGCGCCTATGTGCGCATCATCACCGGCCGCCCGGCGGAGGA
CTGGGCGGCGCTGCGGCGGCAGGGTGCGGACAAGGGAGCCGACAAGGCAAGCGAGGCCCCGGCGCTGCCGCAGGAGGCCT
GCCTTGCCACCGTCGCCGCCCTGCGCAAGGGCGCGCCGGCGCCGCTGGCGGGCGAGCCGGCCAACCCACTGTTCGCCCCC
TGGGGGGTGCAGATCTCCGGCAATTACTCACGCGACATCGCCCTCGCCTCATTCCGCCGGGAGGCGACCCGGCATCCCGC
CGAATTCGGCGACCTGACGCCGGTGATCGTGGCGACGCGGGTGGGCGGGCGCGGCCCGCGCACCTTCTATCGCGTGCGCC
TGCCGGCCCAGACCAAGGCCGAGGGCATGAAAATCTGCGACCGCCTGCGCAAGGCCGGCGGCGCCTGCCTGGTGCTGCCG
AACTGA

Upstream 100 bases:

>100_bases
GCGGCGGCGTCACCGGCGTGACCCTGGTGTCGCTCCATCGCGACTGGATCGAGGCCACCGCGAAGAGCCTCGGCAGCCCG
CTCGGCAGCGGGAGATAGGG

Downstream 100 bases:

>100_bases
GGAGGCGGGCGGGGATATCCCTCGACCGGACGTAATGCGGCTCCCACCCGACCCTCACCCGAACGGGGAGGGATCACCGC
CGCCATCGCCCCTCCCACCG

Product: lytic transglycosylase catalytic

Products: 1,6-Anhydrobond [C]

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MAPAASPRFRRAGRIIAAAVLALLGAGPAFGQSTGEAAPEKPAAASPTPPDPTPRAGGETVDQAICRLIDAAAAKEGLPA
AFLTRLIWRESSFRPHVVSNKGAQGIAQFMPGTAKERGLQNPFDPEAAIPASAALLADLNRRFGNLGLAAAAYNAGPGRV
SGFLNGGGLPEETRAYVRIITGRPAEDWAALRRQGADKGADKASEAPALPQEACLATVAALRKGAPAPLAGEPANPLFAP
WGVQISGNYSRDIALASFRREATRHPAEFGDLTPVIVATRVGGRGPRTFYRVRLPAQTKAEGMKICDRLRKAGGACLVLP
N

Sequences:

>Translated_321_residues
MAPAASPRFRRAGRIIAAAVLALLGAGPAFGQSTGEAAPEKPAAASPTPPDPTPRAGGETVDQAICRLIDAAAAKEGLPA
AFLTRLIWRESSFRPHVVSNKGAQGIAQFMPGTAKERGLQNPFDPEAAIPASAALLADLNRRFGNLGLAAAAYNAGPGRV
SGFLNGGGLPEETRAYVRIITGRPAEDWAALRRQGADKGADKASEAPALPQEACLATVAALRKGAPAPLAGEPANPLFAP
WGVQISGNYSRDIALASFRREATRHPAEFGDLTPVIVATRVGGRGPRTFYRVRLPAQTKAEGMKICDRLRKAGGACLVLP
N
>Mature_320_residues
APAASPRFRRAGRIIAAAVLALLGAGPAFGQSTGEAAPEKPAAASPTPPDPTPRAGGETVDQAICRLIDAAAAKEGLPAA
FLTRLIWRESSFRPHVVSNKGAQGIAQFMPGTAKERGLQNPFDPEAAIPASAALLADLNRRFGNLGLAAAAYNAGPGRVS
GFLNGGGLPEETRAYVRIITGRPAEDWAALRRQGADKGADKASEAPALPQEACLATVAALRKGAPAPLAGEPANPLFAPW
GVQISGNYSRDIALASFRREATRHPAEFGDLTPVIVATRVGGRGPRTFYRVRLPAQTKAEGMKICDRLRKAGGACLVLPN

Specific function: Murein-Degrading Enzyme. Catalyzes The Cleavage Of The Glycosidic Bonds Between N-Acetylmuramic Acid And N- Acetylglucosamine Residues In Peptidoglycan. May Play A Role In Recycling Of Muropeptides During Cell Elongation And/Or Cell Division. [C]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasmic Protein. Tightly Associated With The Murein Sacculus [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 33330; Mature: 33199

Theoretical pI: Translated: 10.69; Mature: 10.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPAASPRFRRAGRIIAAAVLALLGAGPAFGQSTGEAAPEKPAAASPTPPDPTPRAGGET
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
VDQAICRLIDAAAAKEGLPAAFLTRLIWRESSFRPHVVSNKGAQGIAQFMPGTAKERGLQ
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCHHHHCCC
NPFDPEAAIPASAALLADLNRRFGNLGLAAAAYNAGPGRVSGFLNGGGLPEETRAYVRII
CCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHCCCCCCCEEEECCCCCCCHHHHEEEEEE
TGRPAEDWAALRRQGADKGADKASEAPALPQEACLATVAALRKGAPAPLAGEPANPLFAP
CCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
WGVQISGNYSRDIALASFRREATRHPAEFGDLTPVIVATRVGGRGPRTFYRVRLPAQTKA
CCEEECCCCCHHHHHHHHHHHHHCCCHHHCCCCCEEEEECCCCCCCCEEEEEECCCHHHH
EGMKICDRLRKAGGACLVLPN
HHHHHHHHHHHCCCCEEEECC
>Mature Secondary Structure 
APAASPRFRRAGRIIAAAVLALLGAGPAFGQSTGEAAPEKPAAASPTPPDPTPRAGGET
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
VDQAICRLIDAAAAKEGLPAAFLTRLIWRESSFRPHVVSNKGAQGIAQFMPGTAKERGLQ
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCHHHHCCC
NPFDPEAAIPASAALLADLNRRFGNLGLAAAAYNAGPGRVSGFLNGGGLPEETRAYVRII
CCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHCCCCCCCEEEECCCCCCCHHHHEEEEEE
TGRPAEDWAALRRQGADKGADKASEAPALPQEACLATVAALRKGAPAPLAGEPANPLFAP
CCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
WGVQISGNYSRDIALASFRREATRHPAEFGDLTPVIVATRVGGRGPRTFYRVRLPAQTKA
CCEEECCCCCHHHHHHHHHHHHHCCCHHHCCCCCEEEEECCCCCCCCEEEEEECCCHHHH
EGMKICDRLRKAGGACLVLPN
HHHHHHHHHHHCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]