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The map label for this gene is pleD [H]

Identifier: 154247074

GI number: 154247074

Start: 3495161

End: 3496210

Strand: Direct

Name: pleD [H]

Synonym: Xaut_3145

Alternate gene names: 154247074

Gene position: 3495161-3496210 (Clockwise)

Preceding gene: 154247072

Following gene: 154247075

Centisome position: 65.84

GC content: 69.24

Gene sequence:

>1050_bases
ATGGCGCCCGTGCACGTGCCGCGCCCTGCGGGCCGCGACATGCCGCCCGATCGGCCCTCGGACCGGCTCCGGCGCGAAAA
CAGCACTCCGCAACCGGCGACTCGCGCTATATCATTGGGACAAATGAACGATATTCTTGAGCGCCTGCGTCAGCTGGAGC
AGAGCACGCCAGTGCTGGTGGCGCTCTTCGATGCTTCCGATCGCCTGCGCTATGCCAATGCCGCCTTCCGCACCACCATG
TTCATCGCCGAGGGCGAGGACATCACCTGGGAAGAGATGGTGCGGCGCAATTACCACGCCGGGCGCGGCACCGTCATCAA
TACCTCGGACATCGACGAGTGGATCATCGTTCACCGCTCGCGGCGCGGCAAGCAGCGCCACAAGGTGTTCGAGGTCGATT
TTCACGACGGCCGCTGGCTGCTGGTGACCCAGGTGGTGTCGGACGACGGCTGGATGCTGGCCCACGCCGTGGATGTCACC
GCCTTCCGCCCCGACGAGCGGGCCATGCGGCTGGCCCGGGACCGCGCCCTCTATGCCTCCTACACGGACGACCTGACCGG
CGTCGCCAACCGCCGGTTCGTCGGCGCCCGGGTGGCCGACATGCTGGAGCCGGACGGACCGGGCGGCGCCTTCTGCCTCA
TCGACCTCGACCAGTTCAAGGCCATCAACGACCGCTTCGGCCACCAGGCCGGCGACCTGATCCTGCGCGATTTCGCCGCC
CGGATGGTGCGCGAGGTGCGCCGCTCCGACTGCTTCGGCCGGGTGGGCGGGGAAGAGTTCGTGCTGGTGCTGCCGGCGAC
CTCCGCCCGCGCCGCGCGGGGCATCGTGGAGCAGGCGCTGGACGTGGTGCGGCAGGCCCGAGCGCTGACGGCGCAGCCGG
ACTTTGCTTATTCCTTCTCCGCCGGGATCGCGGAAAGCCGGCCGGGCGATACCTTTGCCGACCTCTACAGCCGTGCCGAC
CGCGCGCTCTACGCCGCCAAAATGGCCGGCCGCAGCCGGGTGGAGGTGGAACAGGACCGTCCCCCGGCCGCGGTGGCCTC
GGCGGAATAA

Upstream 100 bases:

>100_bases
AGCGGCAGCATCACCGGCAGCAGGAACATGGTGGCATCAGGCCCACGGCAGCGCCGGCGTGCCGGCCTGTCTTCCATAGC
GCAGGCGGTCGGCCCTTGCC

Downstream 100 bases:

>100_bases
TCGGCTTTTTGTCCTCTATGCGGATTTTAATCCTTGACAGCGTGACGCTGCCCGGCTAGGTTTTGATCATGTTCGAGACG
TGTGCCTGACGGGCCTTCAC

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 349; Mature: 348

Protein sequence:

>349_residues
MAPVHVPRPAGRDMPPDRPSDRLRRENSTPQPATRAISLGQMNDILERLRQLEQSTPVLVALFDASDRLRYANAAFRTTM
FIAEGEDITWEEMVRRNYHAGRGTVINTSDIDEWIIVHRSRRGKQRHKVFEVDFHDGRWLLVTQVVSDDGWMLAHAVDVT
AFRPDERAMRLARDRALYASYTDDLTGVANRRFVGARVADMLEPDGPGGAFCLIDLDQFKAINDRFGHQAGDLILRDFAA
RMVREVRRSDCFGRVGGEEFVLVLPATSARAARGIVEQALDVVRQARALTAQPDFAYSFSAGIAESRPGDTFADLYSRAD
RALYAAKMAGRSRVEVEQDRPPAAVASAE

Sequences:

>Translated_349_residues
MAPVHVPRPAGRDMPPDRPSDRLRRENSTPQPATRAISLGQMNDILERLRQLEQSTPVLVALFDASDRLRYANAAFRTTM
FIAEGEDITWEEMVRRNYHAGRGTVINTSDIDEWIIVHRSRRGKQRHKVFEVDFHDGRWLLVTQVVSDDGWMLAHAVDVT
AFRPDERAMRLARDRALYASYTDDLTGVANRRFVGARVADMLEPDGPGGAFCLIDLDQFKAINDRFGHQAGDLILRDFAA
RMVREVRRSDCFGRVGGEEFVLVLPATSARAARGIVEQALDVVRQARALTAQPDFAYSFSAGIAESRPGDTFADLYSRAD
RALYAAKMAGRSRVEVEQDRPPAAVASAE
>Mature_348_residues
APVHVPRPAGRDMPPDRPSDRLRRENSTPQPATRAISLGQMNDILERLRQLEQSTPVLVALFDASDRLRYANAAFRTTMF
IAEGEDITWEEMVRRNYHAGRGTVINTSDIDEWIIVHRSRRGKQRHKVFEVDFHDGRWLLVTQVVSDDGWMLAHAVDVTA
FRPDERAMRLARDRALYASYTDDLTGVANRRFVGARVADMLEPDGPGGAFCLIDLDQFKAINDRFGHQAGDLILRDFAAR
MVREVRRSDCFGRVGGEEFVLVLPATSARAARGIVEQALDVVRQARALTAQPDFAYSFSAGIAESRPGDTFADLYSRADR
ALYAAKMAGRSRVEVEQDRPPAAVASAE

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87082007, Length=172, Percent_Identity=38.953488372093, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1788381, Length=171, Percent_Identity=35.672514619883, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI87081881, Length=196, Percent_Identity=32.1428571428571, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI145693134, Length=158, Percent_Identity=36.7088607594937, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI87081974, Length=155, Percent_Identity=31.6129032258064, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1786584, Length=180, Percent_Identity=33.8888888888889, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1787816, Length=156, Percent_Identity=29.4871794871795, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI87081977, Length=179, Percent_Identity=31.2849162011173, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787541, Length=169, Percent_Identity=29.585798816568, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787262, Length=177, Percent_Identity=27.683615819209, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1787802, Length=126, Percent_Identity=31.7460317460317, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 39072; Mature: 38940

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPVHVPRPAGRDMPPDRPSDRLRRENSTPQPATRAISLGQMNDILERLRQLEQSTPVLV
CCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
ALFDASDRLRYANAAFRTTMFIAEGEDITWEEMVRRNYHAGRGTVINTSDIDEWIIVHRS
EEECCCCCHHHHHHHHEEEEEEECCCCCCHHHHHHHCCCCCCCCEEECCCCCHHHEEECH
RRGKQRHKVFEVDFHDGRWLLVTQVVSDDGWMLAHAVDVTAFRPDERAMRLARDRALYAS
HCCCCCCCEEEEEECCCCEEEEEEEECCCCEEEEEEEEEEEECCHHHHHHHHHHHHHEEE
YTDDLTGVANRRFVGARVADMLEPDGPGGAFCLIDLDQFKAINDRFGHQAGDLILRDFAA
CCCCHHHHHCCCCHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHCCHHHHHHHHHHHH
RMVREVRRSDCFGRVGGEEFVLVLPATSARAARGIVEQALDVVRQARALTAQPDFAYSFS
HHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECC
AGIAESRPGDTFADLYSRADRALYAAKMAGRSRVEVEQDRPPAAVASAE
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCC
>Mature Secondary Structure 
APVHVPRPAGRDMPPDRPSDRLRRENSTPQPATRAISLGQMNDILERLRQLEQSTPVLV
CCCCCCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
ALFDASDRLRYANAAFRTTMFIAEGEDITWEEMVRRNYHAGRGTVINTSDIDEWIIVHRS
EEECCCCCHHHHHHHHEEEEEEECCCCCCHHHHHHHCCCCCCCCEEECCCCCHHHEEECH
RRGKQRHKVFEVDFHDGRWLLVTQVVSDDGWMLAHAVDVTAFRPDERAMRLARDRALYAS
HCCCCCCCEEEEEECCCCEEEEEEEECCCCEEEEEEEEEEEECCHHHHHHHHHHHHHEEE
YTDDLTGVANRRFVGARVADMLEPDGPGGAFCLIDLDQFKAINDRFGHQAGDLILRDFAA
CCCCHHHHHCCCCHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHCCHHHHHHHHHHHH
RMVREVRRSDCFGRVGGEEFVLVLPATSARAARGIVEQALDVVRQARALTAQPDFAYSFS
HHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECC
AGIAESRPGDTFADLYSRADRALYAAKMAGRSRVEVEQDRPPAAVASAE
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA