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The map label for this gene is dme [H]

Identifier: 154247072

GI number: 154247072

Start: 3491812

End: 3494094

Strand: Direct

Name: dme [H]

Synonym: Xaut_3143

Alternate gene names: 154247072

Gene position: 3491812-3494094 (Clockwise)

Preceding gene: 154247070

Following gene: 154247074

Centisome position: 65.77

GC content: 67.89

Gene sequence:

>2283_bases
ATGGCGTCCAACATTTCCGAGGATCTGCGTTCCGGGGCGCTCGTCTATCATCGCCTGCCGAAGCCCGGGAAACTGGAGAT
CCAGGCCACCAAGCCCCTCGGCAACCAGCGCGACCTCGCGCTCGCCTATTCCCCCGGCGTCGCCGCCGCCTGTGAAGCCA
TCGCCGCCGATCCGCTGCTGGCGGCGGAACTGACCATCCGCGCCAATCTCGTCGCCGTCGTCTCCAACGGAACCGCCGTG
CTCGGCCTCGGCGACATCGGCCCGCTCGCCGGCAAGCCGGTGATGGAGGGCAAGGCCGTCCTGTTCAAGAAGTTCTCCGG
CATCGACGTGTTCGACATCGAAATCGACGCCAAGACCGTCGAGCGCATGGTCGACGTGGTCAGCGCGCTGGAGCCCACCT
TCGGTGGCATCAACCTGGAAGACATCAAGGCTCCCGAGTGCTTCGAGGTGGAAGCCCAGCTGCGCGAGCGCATGAAGATC
CCGGTCTTCCATGACGACCAGCACGGCACCGCCATCATCGTCGGCGCGGCGGTGCGCAACGCCATGGAGGTGGGCAAGCG
CAAGATCGAGGAGCTGAAGATCGTCGCCTCGGGCGCCGGCGCGGCGGCCCTCGCCTCCCTCAACCTGCTGCTCACGCTGG
GTGCCAAGAAGGAGAACATCTGGGTCTGCGACATCGAGGGCGTGGTCTATGACGGCCGCAACACCCTGATGGATCCCTAC
AAGGAGAAGTTCGCCCAGAAGACCGACAAGCGGACCCTGGACGAGGTGATCGAGGGCGCCCACATCTTCCTCGGCCTCTC
CGCCGGCGGCGTGCTGAAGCCGGAGATGGTGAAGAAGATGGCGGACCACCCGCTCATCCTCGCCCTCGCCAATCCGGTGC
CGGAGATCATGCCCGAGGTCGCCCGCGAGGCGCGCCCCGATGCCATGATCTGCACCGGCCGCTCGGACTTCCCCAACCAG
GTCAACAATGTCCTGTGCTTCCCCTACATCTTCCGCGGGGCGCTGGACGTGGGGGCGACCGACATCAACCCCGAGATGAA
GATGGCGGCGGTGGAGGCCATTGCGGCGCTGGCCCGCGAGACCCCCTCCGACGTGGTGGCCCGCGCCTATGGCGGCGAGA
CCCGCGCCTTCGGCGCCGATTCGCTCATTCCCTCGCCGTTCGACCCGCGCCTCATCCTGCGCATCGCGCCGGCGGTGGCG
AAGGCGGCCATGGACACCGGCATCGCCACCCGGCCCATCGCTGACATGGACGCCTATATCGAGCGGCTGGACCATTTCGT
GTTCCGCTCCGGCTTCATCATGCGGCCGCTGTTTGCCCAGGCGAAGCGCAACATCAAGCGCGTGGTCTATGCCGAAGGCG
AGGACGAGCGGGTGCTGCGCGCGGTGCAGGCGGTGGTGGAGGAGGGCATCGCCCGTCCCATCATCGTCGCCCGTCCCAAT
GTGCTGGAGACCCGCCTGAAGCGCTTCGGCCTTTCGGTGCGGCCGGGCCATGACTTCGATCTCATCAACCCGGAAGACGA
TCCGCGTTATCGTGACTATGTGCGCGACTATGTGGAGATCGCCGGCCGCCGCGGCGTGACGCCGGACGCTGCCCGCACCC
TGGTGCGCACCCATCCCACCGTGATCGCGGCGCTGGCCGTGAAGCGCGGCGAGGCGGATGCCATGCTGTGCGGCATCGAG
GGCCGCTTCACCCGCCACCTGCGGCAGGTGCGCGACATCATCGGCCTCGCGCCGGGGGTGAAGGAGCTGGCGGCGCTGTC
GCTGCTCATCACCTCCCACGGCAACTTCTTCATCTGCGACACCCAGATCCAGACCGAGCCCACCGCCGCCGACCTCGCCG
AGATGACCGTGCTGGCCGCCGCCCATGTCCGTCGCTTCGGCCTGGAGCCGCGGGTGGCGCTGCTGTCGCACTCCAATTTC
GGCAGCCACGAGACGCTGTCGGCCAAAAGGGTGCGCTCGGCGCTCTCCATCATCCGCGAGCGTGAGCCGAACCTGGAGGT
GGATGGCGAGATGCAGGCGGATTCCGCCCTGCTGGAGGACATCCGCAAGCGGGTGCTGCCCAACTCGACCCTCACCGGCG
CGGCCAACGTACTGGTGATGCCCGACCTCGACGCCGCCGACATCGCCTTCAACATGCTGAAGGTGCTGGGCGACGCGCTG
CCGGTGGGTCCGATCCTGATGGGCACGGCCAAGCCCGCCCACATCCTCGGCCCGTCGGTCACCGCCCGTGGCATCGTCAA
CATGACCGCCGTCGCGGTGGCCGAGGCCCAGACCGAGGCCTGA

Upstream 100 bases:

>100_bases
CATACCTCAATTTTCATCCCGGCCCGTGACATGAGAGAGCGCGGCGGCTAGGTTGCAGCCCGTTTTGCCGAGCCGGCGGG
CTCGAGGAGGGGGAGGGGAT

Downstream 100 bases:

>100_bases
GCGGGGGCTGCGCTGCTGAAGGCGAGCCTTCCGGAGCGTTGCACCTCCTGATCCCGTCGCCCTCCGGCTTCACCGGAGGG
CCCATCTTGCCGCTTTCCAA

Product: malic enzyme

Products: NA

Alternate protein names: NAD-ME [H]

Number of amino acids: Translated: 760; Mature: 759

Protein sequence:

>760_residues
MASNISEDLRSGALVYHRLPKPGKLEIQATKPLGNQRDLALAYSPGVAAACEAIAADPLLAAELTIRANLVAVVSNGTAV
LGLGDIGPLAGKPVMEGKAVLFKKFSGIDVFDIEIDAKTVERMVDVVSALEPTFGGINLEDIKAPECFEVEAQLRERMKI
PVFHDDQHGTAIIVGAAVRNAMEVGKRKIEELKIVASGAGAAALASLNLLLTLGAKKENIWVCDIEGVVYDGRNTLMDPY
KEKFAQKTDKRTLDEVIEGAHIFLGLSAGGVLKPEMVKKMADHPLILALANPVPEIMPEVAREARPDAMICTGRSDFPNQ
VNNVLCFPYIFRGALDVGATDINPEMKMAAVEAIAALARETPSDVVARAYGGETRAFGADSLIPSPFDPRLILRIAPAVA
KAAMDTGIATRPIADMDAYIERLDHFVFRSGFIMRPLFAQAKRNIKRVVYAEGEDERVLRAVQAVVEEGIARPIIVARPN
VLETRLKRFGLSVRPGHDFDLINPEDDPRYRDYVRDYVEIAGRRGVTPDAARTLVRTHPTVIAALAVKRGEADAMLCGIE
GRFTRHLRQVRDIIGLAPGVKELAALSLLITSHGNFFICDTQIQTEPTAADLAEMTVLAAAHVRRFGLEPRVALLSHSNF
GSHETLSAKRVRSALSIIREREPNLEVDGEMQADSALLEDIRKRVLPNSTLTGAANVLVMPDLDAADIAFNMLKVLGDAL
PVGPILMGTAKPAHILGPSVTARGIVNMTAVAVAEAQTEA

Sequences:

>Translated_760_residues
MASNISEDLRSGALVYHRLPKPGKLEIQATKPLGNQRDLALAYSPGVAAACEAIAADPLLAAELTIRANLVAVVSNGTAV
LGLGDIGPLAGKPVMEGKAVLFKKFSGIDVFDIEIDAKTVERMVDVVSALEPTFGGINLEDIKAPECFEVEAQLRERMKI
PVFHDDQHGTAIIVGAAVRNAMEVGKRKIEELKIVASGAGAAALASLNLLLTLGAKKENIWVCDIEGVVYDGRNTLMDPY
KEKFAQKTDKRTLDEVIEGAHIFLGLSAGGVLKPEMVKKMADHPLILALANPVPEIMPEVAREARPDAMICTGRSDFPNQ
VNNVLCFPYIFRGALDVGATDINPEMKMAAVEAIAALARETPSDVVARAYGGETRAFGADSLIPSPFDPRLILRIAPAVA
KAAMDTGIATRPIADMDAYIERLDHFVFRSGFIMRPLFAQAKRNIKRVVYAEGEDERVLRAVQAVVEEGIARPIIVARPN
VLETRLKRFGLSVRPGHDFDLINPEDDPRYRDYVRDYVEIAGRRGVTPDAARTLVRTHPTVIAALAVKRGEADAMLCGIE
GRFTRHLRQVRDIIGLAPGVKELAALSLLITSHGNFFICDTQIQTEPTAADLAEMTVLAAAHVRRFGLEPRVALLSHSNF
GSHETLSAKRVRSALSIIREREPNLEVDGEMQADSALLEDIRKRVLPNSTLTGAANVLVMPDLDAADIAFNMLKVLGDAL
PVGPILMGTAKPAHILGPSVTARGIVNMTAVAVAEAQTEA
>Mature_759_residues
ASNISEDLRSGALVYHRLPKPGKLEIQATKPLGNQRDLALAYSPGVAAACEAIAADPLLAAELTIRANLVAVVSNGTAVL
GLGDIGPLAGKPVMEGKAVLFKKFSGIDVFDIEIDAKTVERMVDVVSALEPTFGGINLEDIKAPECFEVEAQLRERMKIP
VFHDDQHGTAIIVGAAVRNAMEVGKRKIEELKIVASGAGAAALASLNLLLTLGAKKENIWVCDIEGVVYDGRNTLMDPYK
EKFAQKTDKRTLDEVIEGAHIFLGLSAGGVLKPEMVKKMADHPLILALANPVPEIMPEVAREARPDAMICTGRSDFPNQV
NNVLCFPYIFRGALDVGATDINPEMKMAAVEAIAALARETPSDVVARAYGGETRAFGADSLIPSPFDPRLILRIAPAVAK
AAMDTGIATRPIADMDAYIERLDHFVFRSGFIMRPLFAQAKRNIKRVVYAEGEDERVLRAVQAVVEEGIARPIIVARPNV
LETRLKRFGLSVRPGHDFDLINPEDDPRYRDYVRDYVEIAGRRGVTPDAARTLVRTHPTVIAALAVKRGEADAMLCGIEG
RFTRHLRQVRDIIGLAPGVKELAALSLLITSHGNFFICDTQIQTEPTAADLAEMTVLAAAHVRRFGLEPRVALLSHSNFG
SHETLSAKRVRSALSIIREREPNLEVDGEMQADSALLEDIRKRVLPNSTLTGAANVLVMPDLDAADIAFNMLKVLGDALP
VGPILMGTAKPAHILGPSVTARGIVNMTAVAVAEAQTEA

Specific function: Required for symbiotic nitrogen fixation. Plays a key role in the conversion of malate to acetyl-CoA for efficient tricarboxylic acid cycle function in nitrogen-fixating bacteria [H]

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family [H]

Homologues:

Organism=Homo sapiens, GI4505143, Length=342, Percent_Identity=24.8538011695906, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI62420882, Length=349, Percent_Identity=25.7879656160458, Blast_Score=77, Evalue=6e-14,
Organism=Homo sapiens, GI62420880, Length=349, Percent_Identity=25.7879656160458, Blast_Score=77, Evalue=6e-14,
Organism=Homo sapiens, GI239049447, Length=349, Percent_Identity=25.7879656160458, Blast_Score=77, Evalue=6e-14,
Organism=Homo sapiens, GI4505145, Length=345, Percent_Identity=26.0869565217391, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI270265879, Length=317, Percent_Identity=27.4447949526814, Blast_Score=74, Evalue=6e-13,
Organism=Escherichia coli, GI1788806, Length=757, Percent_Identity=58.2562747688243, Blast_Score=909, Evalue=0.0,
Organism=Escherichia coli, GI1788800, Length=324, Percent_Identity=35.4938271604938, Blast_Score=166, Evalue=4e-42,
Organism=Escherichia coli, GI1788635, Length=349, Percent_Identity=28.6532951289398, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI87081919, Length=299, Percent_Identity=26.4214046822742, Blast_Score=67, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17537199, Length=411, Percent_Identity=24.5742092457421, Blast_Score=67, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6322823, Length=346, Percent_Identity=24.8554913294798, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24646388, Length=345, Percent_Identity=25.7971014492754, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24646386, Length=345, Percent_Identity=25.7971014492754, Blast_Score=77, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR012188
- InterPro:   IPR016040
- InterPro:   IPR002505 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M; PF01515 PTA_PTB [H]

EC number: =1.1.1.39 [H]

Molecular weight: Translated: 81962; Mature: 81831

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASNISEDLRSGALVYHRLPKPGKLEIQATKPLGNQRDLALAYSPGVAAACEAIAADPLL
CCCCCHHHHHCCCEEEEECCCCCCEEEEEECCCCCCCCEEEEECCCHHHHHHHHHCCCHH
AAELTIRANLVAVVSNGTAVLGLGDIGPLAGKPVMEGKAVLFKKFSGIDVFDIEIDAKTV
EEEEEEEEEEEEEEECCEEEEEECCCCCCCCCCCCCCCCEEHHHCCCCEEEEEEECHHHH
ERMVDVVSALEPTFGGINLEDIKAPECFEVEAQLRERMKIPVFHDDQHGTAIIVGAAVRN
HHHHHHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHCCCCCEEECCCCCCEEEEHHHHHH
AMEVGKRKIEELKIVASGAGAAALASLNLLLTLGAKKENIWVCDIEGVVYDGRNTLMDPY
HHHHHHHHHHHHEEEECCCCHHHHHHEEEEEEECCCCCCEEEEEECCEEECCCHHHCCHH
KEKFAQKTDKRTLDEVIEGAHIFLGLSAGGVLKPEMVKKMADHPLILALANPVPEIMPEV
HHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHCCCCEEEEECCCHHHHHHHH
AREARPDAMICTGRSDFPNQVNNVLCFPYIFRGALDVGATDINPEMKMAAVEAIAALARE
HHHCCCCEEEEECCCCCCHHHHCEEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHC
TPSDVVARAYGGETRAFGADSLIPSPFDPRLILRIAPAVAKAAMDTGIATRPIADMDAYI
CHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHCCCCCCCCHHHHHHH
ERLDHFVFRSGFIMRPLFAQAKRNIKRVVYAEGEDERVLRAVQAVVEEGIARPIIVARPN
HHHHHHHHHCCCHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHCCCCCCEEEECCC
VLETRLKRFGLSVRPGHDFDLINPEDDPRYRDYVRDYVEIAGRRGVTPDAARTLVRTHPT
HHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHH
VIAALAVKRGEADAMLCGIEGRFTRHLRQVRDIIGLAPGVKELAALSLLITSHGNFFICD
HHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHEECCCCEEEEE
TQIQTEPTAADLAEMTVLAAAHVRRFGLEPRVALLSHSNFGSHETLSAKRVRSALSIIRE
CEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHH
REPNLEVDGEMQADSALLEDIRKRVLPNSTLTGAANVLVMPDLDAADIAFNMLKVLGDAL
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHC
PVGPILMGTAKPAHILGPSVTARGIVNMTAVAVAEAQTEA
CCCCEEECCCCCCHHCCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ASNISEDLRSGALVYHRLPKPGKLEIQATKPLGNQRDLALAYSPGVAAACEAIAADPLL
CCCCHHHHHCCCEEEEECCCCCCEEEEEECCCCCCCCEEEEECCCHHHHHHHHHCCCHH
AAELTIRANLVAVVSNGTAVLGLGDIGPLAGKPVMEGKAVLFKKFSGIDVFDIEIDAKTV
EEEEEEEEEEEEEEECCEEEEEECCCCCCCCCCCCCCCCEEHHHCCCCEEEEEEECHHHH
ERMVDVVSALEPTFGGINLEDIKAPECFEVEAQLRERMKIPVFHDDQHGTAIIVGAAVRN
HHHHHHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHCCCCCEEECCCCCCEEEEHHHHHH
AMEVGKRKIEELKIVASGAGAAALASLNLLLTLGAKKENIWVCDIEGVVYDGRNTLMDPY
HHHHHHHHHHHHEEEECCCCHHHHHHEEEEEEECCCCCCEEEEEECCEEECCCHHHCCHH
KEKFAQKTDKRTLDEVIEGAHIFLGLSAGGVLKPEMVKKMADHPLILALANPVPEIMPEV
HHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHCCCCEEEEECCCHHHHHHHH
AREARPDAMICTGRSDFPNQVNNVLCFPYIFRGALDVGATDINPEMKMAAVEAIAALARE
HHHCCCCEEEEECCCCCCHHHHCEEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHC
TPSDVVARAYGGETRAFGADSLIPSPFDPRLILRIAPAVAKAAMDTGIATRPIADMDAYI
CHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHCCCCCCCCHHHHHHH
ERLDHFVFRSGFIMRPLFAQAKRNIKRVVYAEGEDERVLRAVQAVVEEGIARPIIVARPN
HHHHHHHHHCCCHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHCCCCCCEEEECCC
VLETRLKRFGLSVRPGHDFDLINPEDDPRYRDYVRDYVEIAGRRGVTPDAARTLVRTHPT
HHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHH
VIAALAVKRGEADAMLCGIEGRFTRHLRQVRDIIGLAPGVKELAALSLLITSHGNFFICD
HHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHEECCCCEEEEE
TQIQTEPTAADLAEMTVLAAAHVRRFGLEPRVALLSHSNFGSHETLSAKRVRSALSIIRE
CEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHH
REPNLEVDGEMQADSALLEDIRKRVLPNSTLTGAANVLVMPDLDAADIAFNMLKVLGDAL
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHC
PVGPILMGTAKPAHILGPSVTARGIVNMTAVAVAEAQTEA
CCCCEEECCCCCCHHCCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9535928; 11481430; 10407149 [H]