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The map label for this gene is yegE [H]

Identifier: 154246903

GI number: 154246903

Start: 3294465

End: 3295340

Strand: Reverse

Name: yegE [H]

Synonym: Xaut_2972

Alternate gene names: 154246903

Gene position: 3295340-3294465 (Counterclockwise)

Preceding gene: 154246904

Following gene: 154246901

Centisome position: 62.07

GC content: 56.05

Gene sequence:

>876_bases
ATGACATTTGGAGGGACTGCCGTGCGCTCGCTGTTCGAGCGGACTTTGGATGCGCTTCCGGACGGCGTCCTACTAACCGA
CTCCGAGCGTCGCGTAGTCTATGCGAATCGCTCTTTTGCTCAGCTCTGGAAGATTCCAGACGAATTGGTGAGCACGAAGA
ACGAGCTCGGCATGCTCACTTTCGTGACGGACCAACTGCTGGACCCGGAGGGTTTCCTTCGCGAGGTGGATCGGCTGCAG
CTGGCCCAGGAGACCTCCGAGGACGAAATTCTGCTCAAGGACGGCCGCATTTTTTCGCGGCGCAGTGTGCCGTTCGATGA
AGGTGGAAGGTTGGCTGCGCGAATCTGGATTTTTACGGATATCACCGAAGCACGCAGCGCCAGTATCGACGCGTTGACGG
GAATACCGAACCGGCATGCCTACTCACGACAATTTCCAGAATATCTCAACACGGCCAACGATGGCCTATTCCGATCCGTC
GCCATTCTCGATATCGACAATTTCAAAAGCTACAATGACAGGCATGGCCATGCGGCTGGAGACGACGTTCTGCGCCAAAT
AGGCACGGTTCTGCGCACGCATTTAACAAATGCCGACGATCTTTTTTTCCGGATCGGCGGCGAGGAGTTTCTCATGGCGT
GTCGTACACGCGCCGCTCTGGACGCACACACTTTTTTTGAGGCATTGCGGAATCGAGTCGGTGCGTTGGCAATTACACAC
CCCGGGAACCAACCCTACGGTGTGGTGACGGTTTCGATGGGGCTTGGGATTTTTGAAGGGCCACAATCGCCGGCCGACGT
CTTTCACCAGGTTGATGCGGCGTTGTATCAATCCAAGCATCTGGGCCGGAACAGGGTAACTATCTGGCGCCTCTAG

Upstream 100 bases:

>100_bases
CATCCTCGATCTATTGGGCAAGGGCGTAAGTGACGAAACTACAACCATTCCCGGATACAATGACCGAGCGCAATTGGGGC
GGACTAGAGGATTGAAGCGC

Downstream 100 bases:

>100_bases
TGCCAGTGTTATCATGGATGGACGACGCCCCGACACGCTATAGTTTCGCGGCAGAGGCGGCCGCGAGCGCGGACTTATCC
TTCACAAGTTCATGGAGGAG

Product: PAS/PAC sensor-containing diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MTFGGTAVRSLFERTLDALPDGVLLTDSERRVVYANRSFAQLWKIPDELVSTKNELGMLTFVTDQLLDPEGFLREVDRLQ
LAQETSEDEILLKDGRIFSRRSVPFDEGGRLAARIWIFTDITEARSASIDALTGIPNRHAYSRQFPEYLNTANDGLFRSV
AILDIDNFKSYNDRHGHAAGDDVLRQIGTVLRTHLTNADDLFFRIGGEEFLMACRTRAALDAHTFFEALRNRVGALAITH
PGNQPYGVVTVSMGLGIFEGPQSPADVFHQVDAALYQSKHLGRNRVTIWRL

Sequences:

>Translated_291_residues
MTFGGTAVRSLFERTLDALPDGVLLTDSERRVVYANRSFAQLWKIPDELVSTKNELGMLTFVTDQLLDPEGFLREVDRLQ
LAQETSEDEILLKDGRIFSRRSVPFDEGGRLAARIWIFTDITEARSASIDALTGIPNRHAYSRQFPEYLNTANDGLFRSV
AILDIDNFKSYNDRHGHAAGDDVLRQIGTVLRTHLTNADDLFFRIGGEEFLMACRTRAALDAHTFFEALRNRVGALAITH
PGNQPYGVVTVSMGLGIFEGPQSPADVFHQVDAALYQSKHLGRNRVTIWRL
>Mature_290_residues
TFGGTAVRSLFERTLDALPDGVLLTDSERRVVYANRSFAQLWKIPDELVSTKNELGMLTFVTDQLLDPEGFLREVDRLQL
AQETSEDEILLKDGRIFSRRSVPFDEGGRLAARIWIFTDITEARSASIDALTGIPNRHAYSRQFPEYLNTANDGLFRSVA
ILDIDNFKSYNDRHGHAAGDDVLRQIGTVLRTHLTNADDLFFRIGGEEFLMACRTRAALDAHTFFEALRNRVGALAITHP
GNQPYGVVTVSMGLGIFEGPQSPADVFHQVDAALYQSKHLGRNRVTIWRL

Specific function: Cyclic-di-GMP is a second messenger which controls cell surface-associated traits in bacteria [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1788381, Length=196, Percent_Identity=32.6530612244898, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI87081881, Length=191, Percent_Identity=36.1256544502618, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI145693134, Length=163, Percent_Identity=30.6748466257669, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787816, Length=164, Percent_Identity=34.7560975609756, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787262, Length=160, Percent_Identity=31.875, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1787541, Length=182, Percent_Identity=30.7692307692308, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI87081977, Length=273, Percent_Identity=27.4725274725275, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787802, Length=140, Percent_Identity=35, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR007895
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013656
- InterPro:   IPR013655 [H]

Pfam domain/function: PF00563 EAL; PF00990 GGDEF; PF05231 MASE1; PF08447 PAS_3; PF08448 PAS_4 [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 32656; Mature: 32525

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS50112 PAS ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFGGTAVRSLFERTLDALPDGVLLTDSERRVVYANRSFAQLWKIPDELVSTKNELGMLT
CCCCHHHHHHHHHHHHHHCCCCEEEECCCCEEEEECCCHHHHHCCHHHHHCCHHHCCCCE
FVTDQLLDPEGFLREVDRLQLAQETSEDEILLKDGRIFSRRSVPFDEGGRLAARIWIFTD
EHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEECCCEEECCCCCCCCCCCEEEEEEEEEE
ITEARSASIDALTGIPNRHAYSRQFPEYLNTANDGLFRSVAILDIDNFKSYNDRHGHAAG
CHHHHCCCCHHHCCCCCCCHHHHHHHHHHHCCCCCHHHEEEEEEECCCCCCCCCCCCCCH
DDVLRQIGTVLRTHLTNADDLFFRIGGEEFLMACRTRAALDAHTFFEALRNRVGALAITH
HHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEC
PGNQPYGVVTVSMGLGIFEGPQSPADVFHQVDAALYQSKHLGRNRVTIWRL
CCCCCEEEEEEEECCCEECCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEC
>Mature Secondary Structure 
TFGGTAVRSLFERTLDALPDGVLLTDSERRVVYANRSFAQLWKIPDELVSTKNELGMLT
CCCHHHHHHHHHHHHHHCCCCEEEECCCCEEEEECCCHHHHHCCHHHHHCCHHHCCCCE
FVTDQLLDPEGFLREVDRLQLAQETSEDEILLKDGRIFSRRSVPFDEGGRLAARIWIFTD
EHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEECCCEEECCCCCCCCCCCEEEEEEEEEE
ITEARSASIDALTGIPNRHAYSRQFPEYLNTANDGLFRSVAILDIDNFKSYNDRHGHAAG
CHHHHCCCCHHHCCCCCCCHHHHHHHHHHHCCCCCHHHEEEEEEECCCCCCCCCCCCCCH
DDVLRQIGTVLRTHLTNADDLFFRIGGEEFLMACRTRAALDAHTFFEALRNRVGALAITH
HHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEC
PGNQPYGVVTVSMGLGIFEGPQSPADVFHQVDAALYQSKHLGRNRVTIWRL
CCCCCEEEEEEEECCCEECCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 6094528; 7984428 [H]