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The map label for this gene is trpI [H]

Identifier: 154246862

GI number: 154246862

Start: 3251386

End: 3252312

Strand: Direct

Name: trpI [H]

Synonym: Xaut_2927

Alternate gene names: 154246862

Gene position: 3251386-3252312 (Clockwise)

Preceding gene: 154246856

Following gene: 154246865

Centisome position: 61.24

GC content: 69.58

Gene sequence:

>927_bases
ATGTCGCAGATCGGCAACCAGCCCTTGCCCTCACTGGTGGCGTTGCGGGCGTTCGAGGCCACGGCCCGCCATCGCAGCAT
GACCCGCGCGGCCGAGGAATTGTGCGTCACCCACGGCGCCGTGAGCCGCCACATCAAGGCGCTGGAGGCGACGCTGGGCG
TGCCTTTGGTCGCGCGCGGCGGTGGCGCCAGCGAGCCGACGCCCGAGGGGCAGCGGCTCGCCGAGGGCCTGTCCACCGCC
TTCGGCCTCATCCACGCCACCCTCGACCGGCTGCGGCCCGGCCCGCTCACGCTCTCCTGCTCCGCCTCCATCATGCTGTG
CTGGCTGATCCCGCGCATGGCGGGCTTCCATCGCCGCAATCCGGACGTGCAGGTGGAGCTGAACATGAATTACGACAAGG
TGGATTTCAGCCGCGACAACATCTCCGTCGCCATCCGCTCCAGCACCATCGTGCCGCCACGGGATGCCATCATCCGCGAG
CTGGGAACGGAATGGATCGGGCCGGTGTGTTCGCCCGATTATCTTGCCGGCCGGGACCTCGCCGCACCGGAAGATCTTGG
AAGTGCGCATCTTCTGTCCACGAATACGCGTCCCGAGGCGTGGCGCGACTGGGCGCAGGCCACTGGCAATGGCGACCTCG
GCCTGAAGGCGCAGCGCAGCTTCGAGCACTTCTACCTGCTGATCCAGGCCGCCGCCTGCGGCCTCGGGGTGGCGGTGGTG
CCGCACATGCTGGCCCTGAGCGAGTTGCAGTCCGGCCGCCTCGTCGCGCCCTTCGGCTTCGTGCCCGGCGGGCGGCACCT
CAGGATGTGGATCGCGCCCCAGGTGGCCTTCCGATCCGATCTCAAGGCGCTGGAATCCTGGCTCCTGGGGGAAATGCGCA
GCTTCGGCCGGCCGCCGGACCCGGCGCTCGCGTCAGTCCGGCCGTGA

Upstream 100 bases:

>100_bases
GTCCGAACAATCGATTTGTTGTCCTTGAGTTGTGAGCTAAACTCACATCATCGATGCTGCGACGCAGCAATAAAGAACGT
GAACGCAGGACCTTGGCTCC

Downstream 100 bases:

>100_bases
TGGCGCGGCGTCTCTGGTCACGCCCGCGCGGCGATTGAGCGTCGTCAAGGCCTATCGGGTGCCGATCCTGCTTTTGCGCA
GGCGGGTGGGCGGCGCCGAG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: TrpBA operon transcriptional activator [H]

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MSQIGNQPLPSLVALRAFEATARHRSMTRAAEELCVTHGAVSRHIKALEATLGVPLVARGGGASEPTPEGQRLAEGLSTA
FGLIHATLDRLRPGPLTLSCSASIMLCWLIPRMAGFHRRNPDVQVELNMNYDKVDFSRDNISVAIRSSTIVPPRDAIIRE
LGTEWIGPVCSPDYLAGRDLAAPEDLGSAHLLSTNTRPEAWRDWAQATGNGDLGLKAQRSFEHFYLLIQAAACGLGVAVV
PHMLALSELQSGRLVAPFGFVPGGRHLRMWIAPQVAFRSDLKALESWLLGEMRSFGRPPDPALASVRP

Sequences:

>Translated_308_residues
MSQIGNQPLPSLVALRAFEATARHRSMTRAAEELCVTHGAVSRHIKALEATLGVPLVARGGGASEPTPEGQRLAEGLSTA
FGLIHATLDRLRPGPLTLSCSASIMLCWLIPRMAGFHRRNPDVQVELNMNYDKVDFSRDNISVAIRSSTIVPPRDAIIRE
LGTEWIGPVCSPDYLAGRDLAAPEDLGSAHLLSTNTRPEAWRDWAQATGNGDLGLKAQRSFEHFYLLIQAAACGLGVAVV
PHMLALSELQSGRLVAPFGFVPGGRHLRMWIAPQVAFRSDLKALESWLLGEMRSFGRPPDPALASVRP
>Mature_307_residues
SQIGNQPLPSLVALRAFEATARHRSMTRAAEELCVTHGAVSRHIKALEATLGVPLVARGGGASEPTPEGQRLAEGLSTAF
GLIHATLDRLRPGPLTLSCSASIMLCWLIPRMAGFHRRNPDVQVELNMNYDKVDFSRDNISVAIRSSTIVPPRDAIIREL
GTEWIGPVCSPDYLAGRDLAAPEDLGSAHLLSTNTRPEAWRDWAQATGNGDLGLKAQRSFEHFYLLIQAAACGLGVAVVP
HMLALSELQSGRLVAPFGFVPGGRHLRMWIAPQVAFRSDLKALESWLLGEMRSFGRPPDPALASVRP

Specific function: Activates the expression of the trpBA genes encoding the two tryptophan synthase subunits. In the absence of the inducer (indoleglycerol phosphate), trpI binds upstream of the trpAB operon, overlapping its own promoter region [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=258, Percent_Identity=36.4341085271318, Blast_Score=138, Evalue=5e-34,
Organism=Escherichia coli, GI1786448, Length=290, Percent_Identity=33.7931034482759, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1788706, Length=272, Percent_Identity=31.9852941176471, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI145693193, Length=306, Percent_Identity=26.797385620915, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1786401, Length=251, Percent_Identity=27.4900398406374, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787128, Length=172, Percent_Identity=31.3953488372093, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1790262, Length=255, Percent_Identity=26.2745098039216, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787589, Length=268, Percent_Identity=24.6268656716418, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33360; Mature: 33229

Theoretical pI: Translated: 8.25; Mature: 8.25

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQIGNQPLPSLVALRAFEATARHRSMTRAAEELCVTHGAVSRHIKALEATLGVPLVARG
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECC
GGASEPTPEGQRLAEGLSTAFGLIHATLDRLRPGPLTLSCSASIMLCWLIPRMAGFHRRN
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHCCC
PDVQVELNMNYDKVDFSRDNISVAIRSSTIVPPRDAIIRELGTEWIGPVCSPDYLAGRDL
CCEEEEEECCCCEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCC
AAPEDLGSAHLLSTNTRPEAWRDWAQATGNGDLGLKAQRSFEHFYLLIQAAACGLGVAVV
CCCCCCCCCEEEECCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHH
PHMLALSELQSGRLVAPFGFVPGGRHLRMWIAPQVAFRSDLKALESWLLGEMRSFGRPPD
HHHHHHHHHCCCCEEECCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCC
PALASVRP
CCCCCCCC
>Mature Secondary Structure 
SQIGNQPLPSLVALRAFEATARHRSMTRAAEELCVTHGAVSRHIKALEATLGVPLVARG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECC
GGASEPTPEGQRLAEGLSTAFGLIHATLDRLRPGPLTLSCSASIMLCWLIPRMAGFHRRN
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHCCC
PDVQVELNMNYDKVDFSRDNISVAIRSSTIVPPRDAIIRELGTEWIGPVCSPDYLAGRDL
CCEEEEEECCCCEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCC
AAPEDLGSAHLLSTNTRPEAWRDWAQATGNGDLGLKAQRSFEHFYLLIQAAACGLGVAVV
CCCCCCCCCEEEECCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHH
PHMLALSELQSGRLVAPFGFVPGGRHLRMWIAPQVAFRSDLKALESWLLGEMRSFGRPPD
HHHHHHHHHCCCCEEECCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCC
PALASVRP
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8423001 [H]