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The map label for this gene is cueO [H]

Identifier: 154246834

GI number: 154246834

Start: 3219856

End: 3221376

Strand: Direct

Name: cueO [H]

Synonym: Xaut_2898

Alternate gene names: 154246834

Gene position: 3219856-3221376 (Clockwise)

Preceding gene: 154246833

Following gene: 154246837

Centisome position: 60.65

GC content: 61.14

Gene sequence:

>1521_bases
ATGCTTCTTTCACGTCGAACGGTTCTGGCGGGAGCTGGCGTTCTTGGATTAGGCATTGCGGGCTTTGCTGCTCGCCCTAT
CCTGTGGCCTAGGAGCGGAACTGACACACAGGCGCTCAAGATCCCACCCCTACTCGACCCGCGGGCGAGCGGGGGATCTG
CCTCGCTTCGCGTGCAGACTGGAACAACCGAGTTCTACCCTGGACGGTTCAGCGAGACGCTCGGATACAATGGGTCTTAC
CTCGGCCCGACCATCCGGGTCAGGCGGGGAGATTCCGTCCAGGTCAACGTCAAGAATGAGCTTGCTGAGACCACCACTGT
GCACTGGCACGGCCTACTCGTTCCAGGCCCGCTGGATGGGGGGCCGCACCAGCCTATCAGTCCAGGTCAGACGTGGCGTC
CGCTGCTCCCGATCGACCAACCGGCTGCCACCTTGATGTATCACTCGCACGTTCACGGGCTCACCGCGGAACAGGTTTAT
CGGGGCCTGGCAGGCGTCTTCATCATTCAGGACGACGAGGAGCAGCGTCTCGGGCTTCCGAACGACTATGGAGTGGACGA
CCTGCCGCTTGTCCTACAGGATCGTCAGTTTGAGGACGGCCTGCTCGTAATGCCAAACGGCATGATGACGCTCATGCAGG
GCAGGCGCGGCGATATCGTTCTGGTAAACGGCACTCCTAACCCCGTGGCTCAGGTGCCTCCACGCCTCGTGCGGCTACGC
ATGGTGAACGCGTCAAACGCGCGCGTCTACGATCTTTCATTCGAGGACAAGAGGGCCTTCCACTGGATCGCGACCGAGGG
CGGCCTTCTTGAACGACCGGTGCAGTTGCAATCGATACGCCTTGCTCCCGGGCAGCGCGCCGAACTGCTGGTGGACTTCT
CGGATCGAAGCCCCGTGGGGCTGCTAACTACCGCTGATTCCAACTCGAGAATAATGGGCATGATGCCTATGATGCAAGAC
TTCGGCCGAGACCGTGTTGCGGGTGGAGCGTTCATCAGGTTTGAGCCGGGCGCGGCTGCTTCGGGAGCCGCATCCGTGCC
TAAGCTGCTCATCGCACAGGAGCGGGTTCAGCCTTCGGCAGCGATACGGCGGCGCCGGCTGGTCCTCAACATGGGTATGG
GCGGAATGATGGGCGGCGGCATGATGGAAGAACAGATGTCGGGCGACACTAGAACGGAAGCCGGTCCCATGGCTGCCCCG
TTCAGCATCAATGGCCGCCCCTTCGACATGCGGCGGATCGATGAGAGCGTGACCCTTGGCAATACGGAGATTTGGGAAGT
CTCGGGCGAGATGATGGCTCACCCGCTCCATATCCACGGCGTCCACTTCGAGGTGCTGAGCAGGGCGGGAAGGAAGCCAG
ACGTCCTGGATCAGGGACTCAGAGATACAGTTCTGGTGCGCGAACCCGCGGAGCTGCTCGTCAAGTTTAGCAAGGCCTCG
CAGGGCTACCCCTTCATGTATCACTGCCACATTCTGGAGCACGAGGACAACGGAATGATGGGCCAATTCTCCGTCGAATA
G

Upstream 100 bases:

>100_bases
GGATGAGGCCGAGATCATGAGAGCTGAACCGGAAGGACCATCGATACAAGGCTAGCTCGCCCTGCTGCATCTTCCGCCGA
TCTGATCAAGGGGTTCCCTC

Downstream 100 bases:

>100_bases
CTATCGGCAATGTTTGGCCCTGTCCGGCTCATTACGTGAATGCTTCTGCGAAACAACGACGCGACGCCCGTCGACAGCTC
GCCCCGAAGAAGGAGTGCAT

Product: Bilirubin oxidase

Products: NA

Alternate protein names: Copper efflux oxidase [H]

Number of amino acids: Translated: 506; Mature: 506

Protein sequence:

>506_residues
MLLSRRTVLAGAGVLGLGIAGFAARPILWPRSGTDTQALKIPPLLDPRASGGSASLRVQTGTTEFYPGRFSETLGYNGSY
LGPTIRVRRGDSVQVNVKNELAETTTVHWHGLLVPGPLDGGPHQPISPGQTWRPLLPIDQPAATLMYHSHVHGLTAEQVY
RGLAGVFIIQDDEEQRLGLPNDYGVDDLPLVLQDRQFEDGLLVMPNGMMTLMQGRRGDIVLVNGTPNPVAQVPPRLVRLR
MVNASNARVYDLSFEDKRAFHWIATEGGLLERPVQLQSIRLAPGQRAELLVDFSDRSPVGLLTTADSNSRIMGMMPMMQD
FGRDRVAGGAFIRFEPGAAASGAASVPKLLIAQERVQPSAAIRRRRLVLNMGMGGMMGGGMMEEQMSGDTRTEAGPMAAP
FSINGRPFDMRRIDESVTLGNTEIWEVSGEMMAHPLHIHGVHFEVLSRAGRKPDVLDQGLRDTVLVREPAELLVKFSKAS
QGYPFMYHCHILEHEDNGMMGQFSVE

Sequences:

>Translated_506_residues
MLLSRRTVLAGAGVLGLGIAGFAARPILWPRSGTDTQALKIPPLLDPRASGGSASLRVQTGTTEFYPGRFSETLGYNGSY
LGPTIRVRRGDSVQVNVKNELAETTTVHWHGLLVPGPLDGGPHQPISPGQTWRPLLPIDQPAATLMYHSHVHGLTAEQVY
RGLAGVFIIQDDEEQRLGLPNDYGVDDLPLVLQDRQFEDGLLVMPNGMMTLMQGRRGDIVLVNGTPNPVAQVPPRLVRLR
MVNASNARVYDLSFEDKRAFHWIATEGGLLERPVQLQSIRLAPGQRAELLVDFSDRSPVGLLTTADSNSRIMGMMPMMQD
FGRDRVAGGAFIRFEPGAAASGAASVPKLLIAQERVQPSAAIRRRRLVLNMGMGGMMGGGMMEEQMSGDTRTEAGPMAAP
FSINGRPFDMRRIDESVTLGNTEIWEVSGEMMAHPLHIHGVHFEVLSRAGRKPDVLDQGLRDTVLVREPAELLVKFSKAS
QGYPFMYHCHILEHEDNGMMGQFSVE
>Mature_506_residues
MLLSRRTVLAGAGVLGLGIAGFAARPILWPRSGTDTQALKIPPLLDPRASGGSASLRVQTGTTEFYPGRFSETLGYNGSY
LGPTIRVRRGDSVQVNVKNELAETTTVHWHGLLVPGPLDGGPHQPISPGQTWRPLLPIDQPAATLMYHSHVHGLTAEQVY
RGLAGVFIIQDDEEQRLGLPNDYGVDDLPLVLQDRQFEDGLLVMPNGMMTLMQGRRGDIVLVNGTPNPVAQVPPRLVRLR
MVNASNARVYDLSFEDKRAFHWIATEGGLLERPVQLQSIRLAPGQRAELLVDFSDRSPVGLLTTADSNSRIMGMMPMMQD
FGRDRVAGGAFIRFEPGAAASGAASVPKLLIAQERVQPSAAIRRRRLVLNMGMGGMMGGGMMEEQMSGDTRTEAGPMAAP
FSINGRPFDMRRIDESVTLGNTEIWEVSGEMMAHPLHIHGVHFEVLSRAGRKPDVLDQGLRDTVLVREPAELLVKFSKAS
QGYPFMYHCHILEHEDNGMMGQFSVE

Specific function: Probably involved in periplasmic detoxification of copper by oxidizing Cu(+) to Cu(2+) and thus preventing its uptake into the cytoplasm. Possesses phenoloxidase and ferroxidase activities and might be involved in the production of polyphenolic compounds

COG id: COG2132

COG function: function code Q; Putative multicopper oxidases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 plastocyanin-like domains [H]

Homologues:

Organism=Escherichia coli, GI1786314, Length=533, Percent_Identity=36.9606003752345, Blast_Score=288, Evalue=8e-79,
Organism=Escherichia coli, GI1789394, Length=508, Percent_Identity=35.6299212598425, Blast_Score=255, Evalue=4e-69,
Organism=Saccharomyces cerevisiae, GI6323703, Length=470, Percent_Identity=23.4042553191489, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6321067, Length=498, Percent_Identity=22.6907630522088, Blast_Score=67, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24650186, Length=234, Percent_Identity=29.0598290598291, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001117
- InterPro:   IPR011706
- InterPro:   IPR011707
- InterPro:   IPR002355
- InterPro:   IPR008972
- InterPro:   IPR006311 [H]

Pfam domain/function: PF00394 Cu-oxidase; PF07731 Cu-oxidase_2; PF07732 Cu-oxidase_3 [H]

EC number: NA

Molecular weight: Translated: 55348; Mature: 55348

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: PS00080 MULTICOPPER_OXIDASE2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLSRRTVLAGAGVLGLGIAGFAARPILWPRSGTDTQALKIPPLLDPRASGGSASLRVQT
CCCCCCEEEECCCHHHHHHHHHHCCCEECCCCCCCCCEEECCCCCCCCCCCCCEEEEEEE
GTTEFYPGRFSETLGYNGSYLGPTIRVRRGDSVQVNVKNELAETTTVHWHGLLVPGPLDG
CCCEECCCCCHHHCCCCCCCCCCEEEEECCCEEEEEEHHHCCCEEEEEEEEEEECCCCCC
GPHQPISPGQTWRPLLPIDQPAATLMYHSHVHGLTAEQVYRGLAGVFIIQDDEEQRLGLP
CCCCCCCCCCCCCCCCCCCCCHHHHEEHHHHCCCCHHHHHHCCCEEEEEECCCHHHCCCC
NDYGVDDLPLVLQDRQFEDGLLVMPNGMMTLMQGRRGDIVLVNGTPNPVAQVPPRLVRLR
CCCCCCCCCEEEECCCCCCCEEECCCCCEEECCCCCCCEEEECCCCCCHHHCCCEEEEEE
MVNASNARVYDLSFEDKRAFHWIATEGGLLERPVQLQSIRLAPGQRAELLVDFSDRSPVG
EECCCCCEEEEECCCCCCEEEEEECCCCCCCCCCEEEEEEECCCCCEEEEEEECCCCCEE
LLTTADSNSRIMGMMPMMQDFGRDRVAGGAFIRFEPGAAASGAASVPKLLIAQERVQPSA
EEEECCCCCEEEEECHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHCCCHH
AIRRRRLVLNMGMGGMMGGGMMEEQMSGDTRTEAGPMAAPFSINGRPFDMRRIDESVTLG
HHHHEEEEEECCCCCCCCCCCCHHHHCCCCCCCCCCCCCCEECCCCCCHHHHCCCEEECC
NTEIWEVSGEMMAHPLHIHGVHFEVLSRAGRKPDVLDQGLRDTVLVREPAELLVKFSKAS
CCEEEEECCCHHCCEEEEEEEEHHHHHHCCCCCCHHHCCCCCEEEEECCHHHHEEHHCCC
QGYPFMYHCHILEHEDNGMMGQFSVE
CCCCEEEEEEEEEECCCCCEEEEECC
>Mature Secondary Structure
MLLSRRTVLAGAGVLGLGIAGFAARPILWPRSGTDTQALKIPPLLDPRASGGSASLRVQT
CCCCCCEEEECCCHHHHHHHHHHCCCEECCCCCCCCCEEECCCCCCCCCCCCCEEEEEEE
GTTEFYPGRFSETLGYNGSYLGPTIRVRRGDSVQVNVKNELAETTTVHWHGLLVPGPLDG
CCCEECCCCCHHHCCCCCCCCCCEEEEECCCEEEEEEHHHCCCEEEEEEEEEEECCCCCC
GPHQPISPGQTWRPLLPIDQPAATLMYHSHVHGLTAEQVYRGLAGVFIIQDDEEQRLGLP
CCCCCCCCCCCCCCCCCCCCCHHHHEEHHHHCCCCHHHHHHCCCEEEEEECCCHHHCCCC
NDYGVDDLPLVLQDRQFEDGLLVMPNGMMTLMQGRRGDIVLVNGTPNPVAQVPPRLVRLR
CCCCCCCCCEEEECCCCCCCEEECCCCCEEECCCCCCCEEEECCCCCCHHHCCCEEEEEE
MVNASNARVYDLSFEDKRAFHWIATEGGLLERPVQLQSIRLAPGQRAELLVDFSDRSPVG
EECCCCCEEEEECCCCCCEEEEEECCCCCCCCCCEEEEEEECCCCCEEEEEEECCCCCEE
LLTTADSNSRIMGMMPMMQDFGRDRVAGGAFIRFEPGAAASGAASVPKLLIAQERVQPSA
EEEECCCCCEEEEECHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHCCCHH
AIRRRRLVLNMGMGGMMGGGMMEEQMSGDTRTEAGPMAAPFSINGRPFDMRRIDESVTLG
HHHHEEEEEECCCCCCCCCCCCHHHHCCCCCCCCCCCCCCEECCCCCCHHHHCCCEEECC
NTEIWEVSGEMMAHPLHIHGVHFEVLSRAGRKPDVLDQGLRDTVLVREPAELLVKFSKAS
CCEEEEECCCHHCCEEEEEEEEHHHHHHCCCCCCHHHCCCCCEEEEECCHHHHEEHHCCC
QGYPFMYHCHILEHEDNGMMGQFSVE
CCCCEEEEEEEEEECCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]