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The map label for this gene is pckA [H]

Identifier: 154246782

GI number: 154246782

Start: 3171641

End: 3173317

Strand: Reverse

Name: pckA [H]

Synonym: Xaut_2843

Alternate gene names: 154246782

Gene position: 3173317-3171641 (Counterclockwise)

Preceding gene: 154246783

Following gene: 154246779

Centisome position: 59.77

GC content: 66.61

Gene sequence:

>1677_bases
ATGGCGTTCATGCGCCGCGCGGGGCCGGCTTCAGGCCGGCGGGAACATCAGGAGGACGAGTCCTTGCTCGAAACCGGTCA
CCGGAACAGCGCCTGCGGCGCCGCCAGCTTCGGTCTGAAACACCTCACCGCGGTTCATTGGAACCTGCTGGAACCCGCGC
TCTACGAGCACGCCCTGGCGAACCGGGAAGCCCGCCTGTCCTCCGGCGGCGCGCTGGTGGCGGAAACCGGCGCCCATACC
GGCCGCAGCCCCAAGGACAAGTTCGTGGTCCGCGACGCCGACACCGAGGCCGAGGTGTGGTGGGACAACAACGGCGCCAT
CTCGCCGGAGCAGTTCGAGACCCTCTACCAGGACTTCCTCGCGGCGGCCGAGGACAAGACCCTCTACGCCCAGGACCTCT
ACGGCGGCGCCGACCCTTCCTGCCGCATCAAGGTGCGCGTCTATACCGAGTTCGCCTGGCACTCGCTGTTCATCCGCACC
ATGCTGCGCCGGCCGGAGCAGGCGGAGCTGGACAGCTTCGTGCCGGAACTCACCATAATCGACCTGCCGTCCTTCAAGGC
GGATCCGGAACGCCACGGGGTCCGCTCGGAAACGGTGATCGCGGTGAACTTCACCCGCGGCATCGTGCTGATCGGCAATT
CGTCCTATGCGGGCGAGATGAAGAAGTCGGTGTTCACGGCGCTGAACTACATCCTGCCGAAAAAGGGCATCATGCCCATG
CACTGCTCGGCGAACGTAGGCGCCGAGGGCGACGTCTGCCTGTTCTTCGGCCTGTCCGGCACCGGCAAGACCACGCTCTC
CGCCGATCCCAACCGCACCCTTCTGGGCGATGACGAGCACGGTTGGAGCCAGAGCGGCGTCTTCAATTTCGAGGGCGGCT
GCTATGCCAAGACCATCCGCCTCTCGGCCGAGGCCGAGCCGGAGATCTTCGCCGCCTCCAACCGCTTCGGCACGGTGCTG
GAGAACGTGGTGCTGGACAAGGTCACCCACGTCCCCGACTTCGACGACGGCAGCCTGACCGAGAACACCCGCTCCTGCTA
TCCCCTGTCGTTCATTCCCAATGCCAGCGCCACCAGCGTCGCGGGCATGCCGAAGAACGTGGTGATGCTCACCTGCGACG
CCTTCGGCGTGCTGCCGCCCATCGCCCGGCTGACCCCGGCGCAGGCCATGTATCACTTCCTGTCCGGCTATACCGCCAAG
GTGGCCGGCACCGAGAAGGGGGTGAAGGACCCGGAAGCCACCTTCTCCACCTGCTTCGGCGCCCCCTTCATGCCGCGCCA
TCCCTCGGTGTACGGCAATCTGCTGCGCGACCTCATCGCCGAGCACCAGGTGGATTGCTGGCTGGTGAACACCGGCTGGA
CCGGCGGCAAGTTCGGCACCGGGCGCCGCATGCCCATCAAGGTCACGCGCACCCTGCTTACGGCGGTGCTGGATGGCTCT
CTGAAAGGCGCCTCCTTCCGCACCGACCCCTATTTCGGCTTCGAGGTGCCAACCTCCGTGCCGGGCATCGAGCCGCACGT
GCTCTATCCCTCCAAGACCTGGGCGGACAAGGCGGAATTCGACGCCACGGCGCGCCGGCTGGTGGAGATGTTCCGGGAGA
ATTTCACACGGTTCGAGAGCCATGTGGACGCCGCCGTCCGCGACGCCCAGCCCCACGTCCGCATCGCCGCCGAGTAG

Upstream 100 bases:

>100_bases
ATCCCGGCAGATCGCGCCGTGAAGGTACACGCTTGGCCTTGTTTCTTTTGGCAACGTCTCCTTATACGCTAAGAGGGCGC
GGCATCATCAAGGACGCAGG

Downstream 100 bases:

>100_bases
GCCCTGAAGCGGGACGGCAGCGTCCCGCCGCCGCATTCTCAAAATGCAAAGGCCCGGCGCCATGGCGCCGGGCCTTGCTG
TTCCAGGGGAAGCTCTGGAT

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 558; Mature: 557

Protein sequence:

>558_residues
MAFMRRAGPASGRREHQEDESLLETGHRNSACGAASFGLKHLTAVHWNLLEPALYEHALANREARLSSGGALVAETGAHT
GRSPKDKFVVRDADTEAEVWWDNNGAISPEQFETLYQDFLAAAEDKTLYAQDLYGGADPSCRIKVRVYTEFAWHSLFIRT
MLRRPEQAELDSFVPELTIIDLPSFKADPERHGVRSETVIAVNFTRGIVLIGNSSYAGEMKKSVFTALNYILPKKGIMPM
HCSANVGAEGDVCLFFGLSGTGKTTLSADPNRTLLGDDEHGWSQSGVFNFEGGCYAKTIRLSAEAEPEIFAASNRFGTVL
ENVVLDKVTHVPDFDDGSLTENTRSCYPLSFIPNASATSVAGMPKNVVMLTCDAFGVLPPIARLTPAQAMYHFLSGYTAK
VAGTEKGVKDPEATFSTCFGAPFMPRHPSVYGNLLRDLIAEHQVDCWLVNTGWTGGKFGTGRRMPIKVTRTLLTAVLDGS
LKGASFRTDPYFGFEVPTSVPGIEPHVLYPSKTWADKAEFDATARRLVEMFRENFTRFESHVDAAVRDAQPHVRIAAE

Sequences:

>Translated_558_residues
MAFMRRAGPASGRREHQEDESLLETGHRNSACGAASFGLKHLTAVHWNLLEPALYEHALANREARLSSGGALVAETGAHT
GRSPKDKFVVRDADTEAEVWWDNNGAISPEQFETLYQDFLAAAEDKTLYAQDLYGGADPSCRIKVRVYTEFAWHSLFIRT
MLRRPEQAELDSFVPELTIIDLPSFKADPERHGVRSETVIAVNFTRGIVLIGNSSYAGEMKKSVFTALNYILPKKGIMPM
HCSANVGAEGDVCLFFGLSGTGKTTLSADPNRTLLGDDEHGWSQSGVFNFEGGCYAKTIRLSAEAEPEIFAASNRFGTVL
ENVVLDKVTHVPDFDDGSLTENTRSCYPLSFIPNASATSVAGMPKNVVMLTCDAFGVLPPIARLTPAQAMYHFLSGYTAK
VAGTEKGVKDPEATFSTCFGAPFMPRHPSVYGNLLRDLIAEHQVDCWLVNTGWTGGKFGTGRRMPIKVTRTLLTAVLDGS
LKGASFRTDPYFGFEVPTSVPGIEPHVLYPSKTWADKAEFDATARRLVEMFRENFTRFESHVDAAVRDAQPHVRIAAE
>Mature_557_residues
AFMRRAGPASGRREHQEDESLLETGHRNSACGAASFGLKHLTAVHWNLLEPALYEHALANREARLSSGGALVAETGAHTG
RSPKDKFVVRDADTEAEVWWDNNGAISPEQFETLYQDFLAAAEDKTLYAQDLYGGADPSCRIKVRVYTEFAWHSLFIRTM
LRRPEQAELDSFVPELTIIDLPSFKADPERHGVRSETVIAVNFTRGIVLIGNSSYAGEMKKSVFTALNYILPKKGIMPMH
CSANVGAEGDVCLFFGLSGTGKTTLSADPNRTLLGDDEHGWSQSGVFNFEGGCYAKTIRLSAEAEPEIFAASNRFGTVLE
NVVLDKVTHVPDFDDGSLTENTRSCYPLSFIPNASATSVAGMPKNVVMLTCDAFGVLPPIARLTPAQAMYHFLSGYTAKV
AGTEKGVKDPEATFSTCFGAPFMPRHPSVYGNLLRDLIAEHQVDCWLVNTGWTGGKFGTGRRMPIKVTRTLLTAVLDGSL
KGASFRTDPYFGFEVPTSVPGIEPHVLYPSKTWADKAEFDATARRLVEMFRENFTRFESHVDAAVRDAQPHVRIAAE

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family [H]

Homologues:

Organism=Escherichia coli, GI1789807, Length=537, Percent_Identity=44.3202979515829, Blast_Score=446, Evalue=1e-126,
Organism=Saccharomyces cerevisiae, GI6322950, Length=521, Percent_Identity=45.10556621881, Blast_Score=465, Evalue=1e-132,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210 [H]

Pfam domain/function: PF01293 PEPCK_ATP [H]

EC number: =4.1.1.49 [H]

Molecular weight: Translated: 61206; Mature: 61074

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFMRRAGPASGRREHQEDESLLETGHRNSACGAASFGLKHLTAVHWNLLEPALYEHALA
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
NREARLSSGGALVAETGAHTGRSPKDKFVVRDADTEAEVWWDNNGAISPEQFETLYQDFL
CCHHHCCCCCEEEEECCCCCCCCCCCCEEEECCCCCEEEEECCCCCCCHHHHHHHHHHHH
AAAEDKTLYAQDLYGGADPSCRIKVRVYTEFAWHSLFIRTMLRRPEQAELDSFVPELTII
HHCCCCEEEHHHHCCCCCCCEEEEEEEEEHHHHHHHHHHHHHCCCCHHHHHHHCCCEEEE
DLPSFKADPERHGVRSETVIAVNFTRGIVLIGNSSYAGEMKKSVFTALNYILPKKGIMPM
ECCCCCCCHHHCCCCCCEEEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCEE
HCSANVGAEGDVCLFFGLSGTGKTTLSADPNRTLLGDDEHGWSQSGVFNFEGGCYAKTIR
ECCCCCCCCCCEEEEEECCCCCCEEECCCCCCCEECCCCCCCCCCCEEECCCCCEEEEEE
LSAEAEPEIFAASNRFGTVLENVVLDKVTHVPDFDDGSLTENTRSCYPLSFIPNASATSV
ECCCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCEE
AGMPKNVVMLTCDAFGVLPPIARLTPAQAMYHFLSGYTAKVAGTEKGVKDPEATFSTCFG
CCCCCCEEEEEECCCCCCCCHHHCCHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHCC
APFMPRHPSVYGNLLRDLIAEHQVDCWLVNTGWTGGKFGTGRRMPIKVTRTLLTAVLDGS
CCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
LKGASFRTDPYFGFEVPTSVPGIEPHVLYPSKTWADKAEFDATARRLVEMFRENFTRFES
CCCCCCCCCCCCCEECCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
HVDAAVRDAQPHVRIAAE
HHHHHHHCCCCCEEEECC
>Mature Secondary Structure 
AFMRRAGPASGRREHQEDESLLETGHRNSACGAASFGLKHLTAVHWNLLEPALYEHALA
CCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
NREARLSSGGALVAETGAHTGRSPKDKFVVRDADTEAEVWWDNNGAISPEQFETLYQDFL
CCHHHCCCCCEEEEECCCCCCCCCCCCEEEECCCCCEEEEECCCCCCCHHHHHHHHHHHH
AAAEDKTLYAQDLYGGADPSCRIKVRVYTEFAWHSLFIRTMLRRPEQAELDSFVPELTII
HHCCCCEEEHHHHCCCCCCCEEEEEEEEEHHHHHHHHHHHHHCCCCHHHHHHHCCCEEEE
DLPSFKADPERHGVRSETVIAVNFTRGIVLIGNSSYAGEMKKSVFTALNYILPKKGIMPM
ECCCCCCCHHHCCCCCCEEEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCEE
HCSANVGAEGDVCLFFGLSGTGKTTLSADPNRTLLGDDEHGWSQSGVFNFEGGCYAKTIR
ECCCCCCCCCCEEEEEECCCCCCEEECCCCCCCEECCCCCCCCCCCEEECCCCCEEEEEE
LSAEAEPEIFAASNRFGTVLENVVLDKVTHVPDFDDGSLTENTRSCYPLSFIPNASATSV
ECCCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCEE
AGMPKNVVMLTCDAFGVLPPIARLTPAQAMYHFLSGYTAKVAGTEKGVKDPEATFSTCFG
CCCCCCEEEEEECCCCCCCCHHHCCHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHCC
APFMPRHPSVYGNLLRDLIAEHQVDCWLVNTGWTGGKFGTGRRMPIKVTRTLLTAVLDGS
CCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
LKGASFRTDPYFGFEVPTSVPGIEPHVLYPSKTWADKAEFDATARRLVEMFRENFTRFES
CCCCCCCCCCCCCEECCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
HVDAAVRDAQPHVRIAAE
HHHHHHHCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA