BacMap

Definition	Xanthobacter autotrophicus Py2 chromosome, complete genome.
Accession	NC_009720
Length	5,308,934

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The map label for this gene is ydfG [H]

Identifier: 154246751

GI number: 154246751

Start: 3132901

End: 3133674

Strand: Direct

Name: ydfG [H]

Synonym: Xaut_2812

Alternate gene names: 154246751

Gene position: 3132901-3133674 (Clockwise)

Preceding gene: 154246749

Following gene: 154246752

Centisome position: 59.01

GC content: 67.96

Gene sequence:

>774_bases
ATGTCCATGCTCGATCCCAGCACCCTGACCCTTCTTGTCACCGGCGCCACCTCCGGCATCGGCGCGGCGACGGTGCGCCG
CTTCGCCAGCGCCGGCGCGCGGGTGGTGGCGGTGGGCCGCCGGGCGGAGCGGCTGGAGGCGCTGAAGGCCGAGTTCGGTG
ACCTCATCCATCCCCTGGTGCTGGACGTGCGCGACGAGAAGGCCACCTTCGAGGCCTTCGCCAGCCTGCCGGCACCGTTC
AACGCCTACAACGTGGTGTTTGCCAATGCCGGCCTCGCCCTCGGCCTCGAGCCCGCCTATGCGGCGGATCTGGAGGAGTG
GAAGACCATGGTGGACACCAACATCACCGGCCTTCTGGTGACCGTGCGGGCAAGCCTGCCGGCCATGATCGAGCGCAAGC
AGGGCCACCTGGTGTTCACCGGCTCCATCGCCGGCGACTATTCCTATCCCGGCGCCAACGTCTACGGCGCCACCAAGGCC
TTCGTGAAGCAGTTCTCGCTGAACGTGTGGGCGGATGTGGCCGGCACCGGCGTGCGCGTCACCAACATCGAGCCCGGCCT
CACCGAGACCGAGTTCTCCATGGTCCGCTTCGGCGGCGACAAGGAGCGGGCCGACAAGGTCTATGCCGGCGCCACCCCCA
TGACCGGCGAGGACATCGCCGAGCAGGTGTTCTTCGCCTGCACCCTGCCGCGGCATGTGAACATCAACCGCATCCAGTCC
ATGTCCTCCCAGCAGTCCTTCGGTCCGCTGGCGATCAAGCGCGACCCGGTGTGA

Upstream 100 bases:

>100_bases
TAGCGGGTGCCAGATGGACGCGATGGTTAAGGCGGGTTAATCAGCAGGCCCCTACCTTTTTGCCAGATTCTTCTCCCCCT
TCGCTTGCCGGAGCCTGCCC

Downstream 100 bases:

>100_bases
TAGGTGGGCCGGGGTCCGGCCCGGGGCGGTGAAATCGGGTGGGCGCGGCTTGCCGGCTCTGGTCAGATGGGCCACGCTCG
ACCTGAAGGAGATGCCCCGT

Product: short-chain dehydrogenase/reductase SDR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MSMLDPSTLTLLVTGATSGIGAATVRRFASAGARVVAVGRRAERLEALKAEFGDLIHPLVLDVRDEKATFEAFASLPAPF
NAYNVVFANAGLALGLEPAYAADLEEWKTMVDTNITGLLVTVRASLPAMIERKQGHLVFTGSIAGDYSYPGANVYGATKA
FVKQFSLNVWADVAGTGVRVTNIEPGLTETEFSMVRFGGDKERADKVYAGATPMTGEDIAEQVFFACTLPRHVNINRIQS
MSSQQSFGPLAIKRDPV

Sequences:

>Translated_257_residues
MSMLDPSTLTLLVTGATSGIGAATVRRFASAGARVVAVGRRAERLEALKAEFGDLIHPLVLDVRDEKATFEAFASLPAPF
NAYNVVFANAGLALGLEPAYAADLEEWKTMVDTNITGLLVTVRASLPAMIERKQGHLVFTGSIAGDYSYPGANVYGATKA
FVKQFSLNVWADVAGTGVRVTNIEPGLTETEFSMVRFGGDKERADKVYAGATPMTGEDIAEQVFFACTLPRHVNINRIQS
MSSQQSFGPLAIKRDPV
>Mature_256_residues
SMLDPSTLTLLVTGATSGIGAATVRRFASAGARVVAVGRRAERLEALKAEFGDLIHPLVLDVRDEKATFEAFASLPAPFN
AYNVVFANAGLALGLEPAYAADLEEWKTMVDTNITGLLVTVRASLPAMIERKQGHLVFTGSIAGDYSYPGANVYGATKAF
VKQFSLNVWADVAGTGVRVTNIEPGLTETEFSMVRFGGDKERADKVYAGATPMTGEDIAEQVFFACTLPRHVNINRIQSM
SSQQSFGPLAIKRDPV

Specific function: Catalyzes the NADP-dependent oxidation of L-allo- threonine and L-serine [H]

COG id: COG4221

COG function: function code R; Short-chain alcohol dehydrogenase of unknown specificity

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI109715829, Length=246, Percent_Identity=30.4878048780488, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI20149619, Length=203, Percent_Identity=30.5418719211823, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI157739938, Length=184, Percent_Identity=27.1739130434783, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI7706318, Length=188, Percent_Identity=30.3191489361702, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI223718074, Length=206, Percent_Identity=30.5825242718447, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI40254992, Length=182, Percent_Identity=28.021978021978, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI7657478, Length=190, Percent_Identity=28.9473684210526, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI7705855, Length=190, Percent_Identity=24.2105263157895, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI40548397, Length=196, Percent_Identity=27.0408163265306, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI214010158, Length=196, Percent_Identity=27.0408163265306, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI214010156, Length=196, Percent_Identity=27.0408163265306, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI22758144, Length=196, Percent_Identity=27.0408163265306, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI32483357, Length=237, Percent_Identity=26.5822784810127, Blast_Score=65, Evalue=8e-11,
Organism=Escherichia coli, GI1787820, Length=247, Percent_Identity=51.8218623481781, Blast_Score=261, Evalue=3e-71,
Organism=Escherichia coli, GI1787335, Length=193, Percent_Identity=29.5336787564767, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI87082100, Length=189, Percent_Identity=31.7460317460317, Blast_Score=77, Evalue=8e-16,
Organism=Escherichia coli, GI1786812, Length=249, Percent_Identity=24.8995983935743, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1787526, Length=212, Percent_Identity=27.8301886792453, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1786701, Length=194, Percent_Identity=27.319587628866, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI2367175, Length=221, Percent_Identity=28.0542986425339, Blast_Score=61, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI17559104, Length=234, Percent_Identity=27.7777777777778, Blast_Score=84, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17531453, Length=214, Percent_Identity=28.9719626168224, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17563726, Length=235, Percent_Identity=28.0851063829787, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17560220, Length=194, Percent_Identity=27.8350515463918, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17562908, Length=191, Percent_Identity=28.7958115183246, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17565030, Length=219, Percent_Identity=29.6803652968037, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17562904, Length=199, Percent_Identity=28.643216080402, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17560332, Length=208, Percent_Identity=26.9230769230769, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71994604, Length=231, Percent_Identity=28.1385281385281, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17562906, Length=191, Percent_Identity=28.2722513089005, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17562990, Length=208, Percent_Identity=27.8846153846154, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17560676, Length=201, Percent_Identity=26.3681592039801, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71994600, Length=235, Percent_Identity=25.9574468085106, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17560150, Length=208, Percent_Identity=26.9230769230769, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17538486, Length=207, Percent_Identity=27.536231884058, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI72000259, Length=192, Percent_Identity=29.1666666666667, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17538182, Length=193, Percent_Identity=26.9430051813472, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17562910, Length=195, Percent_Identity=27.6923076923077, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI193204405, Length=192, Percent_Identity=26.5625, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6323882, Length=234, Percent_Identity=34.1880341880342, Blast_Score=136, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6322067, Length=188, Percent_Identity=31.3829787234043, Blast_Score=80, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6322227, Length=195, Percent_Identity=26.1538461538462, Blast_Score=69, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6322226, Length=187, Percent_Identity=24.0641711229947, Blast_Score=63, Evalue=6e-11,
Organism=Drosophila melanogaster, GI116007236, Length=241, Percent_Identity=29.045643153527, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI21358495, Length=234, Percent_Identity=28.6324786324786, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24641232, Length=246, Percent_Identity=30.8943089430894, Blast_Score=95, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24663911, Length=246, Percent_Identity=28.8617886178862, Blast_Score=91, Evalue=8e-19,
Organism=Drosophila melanogaster, GI23397609, Length=188, Percent_Identity=35.6382978723404, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24644339, Length=200, Percent_Identity=31, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24641388, Length=247, Percent_Identity=28.7449392712551, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI28571387, Length=242, Percent_Identity=27.6859504132231, Blast_Score=85, Evalue=4e-17,
Organism=Drosophila melanogaster, GI281364494, Length=246, Percent_Identity=29.2682926829268, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21355319, Length=221, Percent_Identity=26.6968325791855, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24648944, Length=238, Percent_Identity=29.4117647058824, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24643142, Length=182, Percent_Identity=32.4175824175824, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI116007234, Length=242, Percent_Identity=28.5123966942149, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI21357041, Length=196, Percent_Identity=32.1428571428571, Blast_Score=81, Evalue=7e-16,
Organism=Drosophila melanogaster, GI28571526, Length=200, Percent_Identity=31.5, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24640442, Length=193, Percent_Identity=29.5336787564767, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI116007238, Length=160, Percent_Identity=32.5, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24648693, Length=243, Percent_Identity=27.1604938271605, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21355889, Length=243, Percent_Identity=27.1604938271605, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI161076933, Length=195, Percent_Identity=28.7179487179487, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24584748, Length=195, Percent_Identity=28.7179487179487, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI17737361, Length=190, Percent_Identity=28.4210526315789, Blast_Score=67, Evalue=9e-12,
Organism=Drosophila melanogaster, GI116007232, Length=162, Percent_Identity=30.8641975308642, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: 1300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 27522; Mature: 27391

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMLDPSTLTLLVTGATSGIGAATVRRFASAGARVVAVGRRAERLEALKAEFGDLIHPLV
CCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHH
LDVRDEKATFEAFASLPAPFNAYNVVFANAGLALGLEPAYAADLEEWKTMVDTNITGLLV
EECCCCHHHHHHHHCCCCCCCCEEEEEECCCEEECCCCHHHHHHHHHHHHHHCCCCEEEE
TVRASLPAMIERKQGHLVFTGSIAGDYSYPGANVYGATKAFVKQFSLNVWADVAGTGVRV
EEECCCCHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCEEEECCCCCEEE
TNIEPGLTETEFSMVRFGGDKERADKVYAGATPMTGEDIAEQVFFACTLPRHVNINRIQS
EECCCCCCCCCEEHEEECCCHHHHCEEEECCCCCCHHHHHHHHHHHCCCCCCCCHHHHHH
MSSQQSFGPLAIKRDPV
HHHHCCCCCEEEECCCC
>Mature Secondary Structure 
SMLDPSTLTLLVTGATSGIGAATVRRFASAGARVVAVGRRAERLEALKAEFGDLIHPLV
CCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHH
LDVRDEKATFEAFASLPAPFNAYNVVFANAGLALGLEPAYAADLEEWKTMVDTNITGLLV
EECCCCHHHHHHHHCCCCCCCCEEEEEECCCEEECCCCHHHHHHHHHHHHHHCCCCEEEE
TVRASLPAMIERKQGHLVFTGSIAGDYSYPGANVYGATKAFVKQFSLNVWADVAGTGVRV
EEECCCCHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCEEEECCCCCEEE
TNIEPGLTETEFSMVRFGGDKERADKVYAGATPMTGEDIAEQVFFACTLPRHVNINRIQS
EECCCCCCCCCEEHEEECCCHHHHCEEEECCCCCCHHHHHHHHHHHCCCCCCCCHHHHHH
MSSQQSFGPLAIKRDPV
HHHHCCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]