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The map label for this gene is potH [H]

Identifier: 154246519

GI number: 154246519

Start: 2869119

End: 2869970

Strand: Direct

Name: potH [H]

Synonym: Xaut_2578

Alternate gene names: 154246519

Gene position: 2869119-2869970 (Clockwise)

Preceding gene: 154246518

Following gene: 154246520

Centisome position: 54.04

GC content: 65.61

Gene sequence:

>852_bases
ATGAACAAGCGCCCTTTCCTTGCCCTGTTCGTCACCCCCGCCGTGCTGGCGGCCCTCGCCTCGGCGGCGGCGCTGGCGGC
GATCCTGCAGTACGCGTTCCGCGCCTATGTGCCGGGATCGCTCGATCCGGGCGGCTTCTCGCTGGCGAATTTCGAGGCGC
TGCTGCGCCCGCTCTATGCGCAGGTGTTCGTGCAGACCGTGTGGATCTGCCTGCTCACTGCCTTCATCACCCTTGTGGTG
GCCTATCCGCTGGCCTTCGCGCTGGTGCGCACGCGCAACGTGGCGCTGAAGTCGTTCCTGCTGATCACGGCGGTGACGCC
ATTGTTCCTCGGCGAAGTGGTGCGGACCTATTCCTGGATCATCGTTCTGGGGAACACCGGCTTCATCAATTCCATCCTCA
TGGGCCTCGGCCTCGTAGAACGGCCGGTGCAGATGATGTTCACCACCTTTGGCGTGGTGGTGGCGCTGGTGCATGTGACC
CTGCCGGTGATGGTCATCATGCTCGCCGCCGCGCTCTCCCACATCGACGTGGACTACGAGAAGGCGGCCACCTCGCTCGG
CGCCGGGCCGGTGAAGGCGTTCCTCACGGTGACGCTGCCGCTCTCCATGCCGGGCGTGGTGGCGGGCGTCTCCACCGCCT
TCGCCTGGACCTTTTCCGCCTTCGCGACTCCCCAGATGATCGGCGGCGGACGGGTGAGCATGATCTCGACCCTCGTCTAT
CAGCTGGGCTTTGCCTCCTTCAATTTCCCGTTCGCGGCGGCGCTGTCCATCACCGGCATCGCGCTCACCTTCGCGGTGCT
GGCCATGGCGAAGCAGGCCATCCGCCCGCTGGAAAAGATCGGAGCCCACTGA

Upstream 100 bases:

>100_bases
TCGGGCATCGCATCACCGCGGAGGGGAGGGTGGGTCCCCTGCCCACTCTCCCCTCCGCCCCGGCCGGGACGCCGGGACGG
CTCAAGAATGGTCGAATGGC

Downstream 100 bases:

>100_bases
TGCGCCGCCGTCTGATCCCCGCGCGCGTGGGCGAGCGCATCCTGAAGGTCGCCGGCTGGAGCGTGGTGGCGCTCATCATC
GCCTTCATCATCCTGCCGGC

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MNKRPFLALFVTPAVLAALASAAALAAILQYAFRAYVPGSLDPGGFSLANFEALLRPLYAQVFVQTVWICLLTAFITLVV
AYPLAFALVRTRNVALKSFLLITAVTPLFLGEVVRTYSWIIVLGNTGFINSILMGLGLVERPVQMMFTTFGVVVALVHVT
LPVMVIMLAAALSHIDVDYEKAATSLGAGPVKAFLTVTLPLSMPGVVAGVSTAFAWTFSAFATPQMIGGGRVSMISTLVY
QLGFASFNFPFAAALSITGIALTFAVLAMAKQAIRPLEKIGAH

Sequences:

>Translated_283_residues
MNKRPFLALFVTPAVLAALASAAALAAILQYAFRAYVPGSLDPGGFSLANFEALLRPLYAQVFVQTVWICLLTAFITLVV
AYPLAFALVRTRNVALKSFLLITAVTPLFLGEVVRTYSWIIVLGNTGFINSILMGLGLVERPVQMMFTTFGVVVALVHVT
LPVMVIMLAAALSHIDVDYEKAATSLGAGPVKAFLTVTLPLSMPGVVAGVSTAFAWTFSAFATPQMIGGGRVSMISTLVY
QLGFASFNFPFAAALSITGIALTFAVLAMAKQAIRPLEKIGAH
>Mature_283_residues
MNKRPFLALFVTPAVLAALASAAALAAILQYAFRAYVPGSLDPGGFSLANFEALLRPLYAQVFVQTVWICLLTAFITLVV
AYPLAFALVRTRNVALKSFLLITAVTPLFLGEVVRTYSWIIVLGNTGFINSILMGLGLVERPVQMMFTTFGVVVALVHVT
LPVMVIMLAAALSHIDVDYEKAATSLGAGPVKAFLTVTLPLSMPGVVAGVSTAFAWTFSAFATPQMIGGGRVSMISTLVY
QLGFASFNFPFAAALSITGIALTFAVLAMAKQAIRPLEKIGAH

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=213, Percent_Identity=31.4553990610329, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1787369, Length=234, Percent_Identity=29.9145299145299, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1787713, Length=239, Percent_Identity=28.4518828451883, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1786980, Length=162, Percent_Identity=32.0987654320988, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30024; Mature: 30024

Theoretical pI: Translated: 10.18; Mature: 10.18

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKRPFLALFVTPAVLAALASAAALAAILQYAFRAYVPGSLDPGGFSLANFEALLRPLYA
CCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
QVFVQTVWICLLTAFITLVVAYPLAFALVRTRNVALKSFLLITAVTPLFLGEVVRTYSWI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
IVLGNTGFINSILMGLGLVERPVQMMFTTFGVVVALVHVTLPVMVIMLAAALSHIDVDYE
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
KAATSLGAGPVKAFLTVTLPLSMPGVVAGVSTAFAWTFSAFATPQMIGGGRVSMISTLVY
HHHHHCCCCHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHHHHHHH
QLGFASFNFPFAAALSITGIALTFAVLAMAKQAIRPLEKIGAH
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNKRPFLALFVTPAVLAALASAAALAAILQYAFRAYVPGSLDPGGFSLANFEALLRPLYA
CCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
QVFVQTVWICLLTAFITLVVAYPLAFALVRTRNVALKSFLLITAVTPLFLGEVVRTYSWI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
IVLGNTGFINSILMGLGLVERPVQMMFTTFGVVVALVHVTLPVMVIMLAAALSHIDVDYE
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
KAATSLGAGPVKAFLTVTLPLSMPGVVAGVSTAFAWTFSAFATPQMIGGGRVSMISTLVY
HHHHHCCCCHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHHHHHHH
QLGFASFNFPFAAALSITGIALTFAVLAMAKQAIRPLEKIGAH
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]