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The map label for this gene is potD-A [H]

Identifier: 154246518

GI number: 154246518

Start: 2867877

End: 2868908

Strand: Direct

Name: potD-A [H]

Synonym: Xaut_2577

Alternate gene names: 154246518

Gene position: 2867877-2868908 (Clockwise)

Preceding gene: 154246517

Following gene: 154246519

Centisome position: 54.02

GC content: 63.47

Gene sequence:

>1032_bases
ATGAAAGGCAAGTTCCTTGCCCTCGGCCTCGCGCTGGCCGCCCTCGCGGCGGGTCCGGCAAGCGCCGCGGAAAAGCTGGT
GGTGAGCACGTGGGGCGGCAGCTTCCGTGACCTCATCGCCGACACCATCGCCAAGAAGTTCACCGCCGAAACCGGCGTGG
AGGTGGAATACATCACCGGCGGCACCATCGACCGGCTGAACAAGGCGAAGCTCGCCAAGGGGGCGCCCGAAAGCGACATC
ACTTTCACCACGGCGCACGTGGGCTGGCTCTATGCCACCGACGACCTGTTCGAGACGCTGGATCTGGCCAAGATCCCCAA
TGCGTCGAAGCTGGTGCCGCAGGCCAAGGTGAGCCCCTATCACATCGGCGCCTGGGCCTATGTCTACACCATCGGCTACC
GGCCCGACCTGACGCCGAAGAACATCACCTTCTCGTCCTGGGCCGACCTGTGGAACCCCGAGCTGAAGGGCATGATCGCG
GCCCCCGATTTCGACCCCAGCCACATCATCGCGGTGTCCGCGCTGCTGGCCGGCGGCTCGCCTGCCGACTGGCAGAAGGG
CGAGCAGAAGCTCTTGGCGCTGAAGCCGAATTTCAAGGCCTTCTACACCAACGACGCCAATTCCCAGCAGCTCATCGCCA
CCGGTGAAACGCCGGTCCAGATCATGCTGTCGATGAATGCCTATTACATGATCGCGCAGGGCGTTCCGATCAAGCTGGTG
ATCCCGAAGGAAGGCGGCGTTCTCGGCATCGACACCATGGCCATCATGAAGGGCTCCAAGAAGCAGGAGCTGGCGCAGAA
ATTCATCAACACGGCGCTTGATCCCGAGGTGCAGGGCCAGATCGCCGAGCTGAAGAAGGGCTCGCCCACCGTGCTGGGGG
CCAAGGTGAGCGCCGAGACGGCCAAGCTTCCCGGCGTCTTCACCACCGCCGAGCAGTGGGAGAAGGAGACCATCATCATC
GACCACAAGCTGCGCGCGGAGAAGACCGCCGAGTGGCGCAAGTGGTTCTCCGAGAACATGATCGCCAAGTAG

Upstream 100 bases:

>100_bases
CCTCTATCTACGTACATTTACGTAAATGTCTCGCGCCGGATGGCGCGGGGCAGGTTCCTAAAATCAACCGCCGCAAAGGC
GGATCCAGAGGAGAACACGG

Downstream 100 bases:

>100_bases
AGCGGGGTCACCAAGTCGTCGTCGACATTTTCCGGTCATGGCGGGCGCGGTTCGGCGTCCGCCCGCGATGTGAGGCGGAC
GCCTGGCCCCCAGACTGCCC

Product: extracellular solute-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MKGKFLALGLALAALAAGPASAAEKLVVSTWGGSFRDLIADTIAKKFTAETGVEVEYITGGTIDRLNKAKLAKGAPESDI
TFTTAHVGWLYATDDLFETLDLAKIPNASKLVPQAKVSPYHIGAWAYVYTIGYRPDLTPKNITFSSWADLWNPELKGMIA
APDFDPSHIIAVSALLAGGSPADWQKGEQKLLALKPNFKAFYTNDANSQQLIATGETPVQIMLSMNAYYMIAQGVPIKLV
IPKEGGVLGIDTMAIMKGSKKQELAQKFINTALDPEVQGQIAELKKGSPTVLGAKVSAETAKLPGVFTTAEQWEKETIII
DHKLRAEKTAEWRKWFSENMIAK

Sequences:

>Translated_343_residues
MKGKFLALGLALAALAAGPASAAEKLVVSTWGGSFRDLIADTIAKKFTAETGVEVEYITGGTIDRLNKAKLAKGAPESDI
TFTTAHVGWLYATDDLFETLDLAKIPNASKLVPQAKVSPYHIGAWAYVYTIGYRPDLTPKNITFSSWADLWNPELKGMIA
APDFDPSHIIAVSALLAGGSPADWQKGEQKLLALKPNFKAFYTNDANSQQLIATGETPVQIMLSMNAYYMIAQGVPIKLV
IPKEGGVLGIDTMAIMKGSKKQELAQKFINTALDPEVQGQIAELKKGSPTVLGAKVSAETAKLPGVFTTAEQWEKETIII
DHKLRAEKTAEWRKWFSENMIAK
>Mature_343_residues
MKGKFLALGLALAALAAGPASAAEKLVVSTWGGSFRDLIADTIAKKFTAETGVEVEYITGGTIDRLNKAKLAKGAPESDI
TFTTAHVGWLYATDDLFETLDLAKIPNASKLVPQAKVSPYHIGAWAYVYTIGYRPDLTPKNITFSSWADLWNPELKGMIA
APDFDPSHIIAVSALLAGGSPADWQKGEQKLLALKPNFKAFYTNDANSQQLIATGETPVQIMLSMNAYYMIAQGVPIKLV
IPKEGGVLGIDTMAIMKGSKKQELAQKFINTALDPEVQGQIAELKKGSPTVLGAKVSAETAKLPGVFTTAEQWEKETIII
DHKLRAEKTAEWRKWFSENMIAK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=312, Percent_Identity=28.2051282051282, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1787078, Length=356, Percent_Identity=27.5280898876405, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 37156; Mature: 37156

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGKFLALGLALAALAAGPASAAEKLVVSTWGGSFRDLIADTIAKKFTAETGVEVEYITG
CCCCCHHHHHHHHHHHCCCHHHHCEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEECC
GTIDRLNKAKLAKGAPESDITFTTAHVGWLYATDDLFETLDLAKIPNASKLVPQAKVSPY
CCHHHHCHHHHHCCCCCCCEEEEEEEEEEEEECHHHHHHHHHHHCCCHHHCCCCCCCCCE
HIGAWAYVYTIGYRPDLTPKNITFSSWADLWNPELKGMIAAPDFDPSHIIAVSALLAGGS
EECEEEEEEEECCCCCCCCCCCCHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHCCCC
PADWQKGEQKLLALKPNFKAFYTNDANSQQLIATGETPVQIMLSMNAYYMIAQGVPIKLV
CCCCCCCCCEEEEECCCCEEEEECCCCCCEEEEECCCCEEEEEECCEEEEEECCCEEEEE
IPKEGGVLGIDTMAIMKGSKKQELAQKFINTALDPEVQGQIAELKKGSPTVLGAKVSAET
EECCCCEEEEEHHHHHCCCCHHHHHHHHHHHCCCHHHCCHHHHHCCCCCEEEEEEECCCH
AKLPGVFTTAEQWEKETIIIDHKLRAEKTAEWRKWFSENMIAK
HHCCCEEECHHHCCCCEEEEECCCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKGKFLALGLALAALAAGPASAAEKLVVSTWGGSFRDLIADTIAKKFTAETGVEVEYITG
CCCCCHHHHHHHHHHHCCCHHHHCEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEECC
GTIDRLNKAKLAKGAPESDITFTTAHVGWLYATDDLFETLDLAKIPNASKLVPQAKVSPY
CCHHHHCHHHHHCCCCCCCEEEEEEEEEEEEECHHHHHHHHHHHCCCHHHCCCCCCCCCE
HIGAWAYVYTIGYRPDLTPKNITFSSWADLWNPELKGMIAAPDFDPSHIIAVSALLAGGS
EECEEEEEEEECCCCCCCCCCCCHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHCCCC
PADWQKGEQKLLALKPNFKAFYTNDANSQQLIATGETPVQIMLSMNAYYMIAQGVPIKLV
CCCCCCCCCEEEEECCCCEEEEECCCCCCEEEEECCCCEEEEEECCEEEEEECCCEEEEE
IPKEGGVLGIDTMAIMKGSKKQELAQKFINTALDPEVQGQIAELKKGSPTVLGAKVSAET
EECCCCEEEEEHHHHHCCCCHHHHHHHHHHHCCCHHHCCHHHHHCCCCCEEEEEEECCCH
AKLPGVFTTAEQWEKETIIIDHKLRAEKTAEWRKWFSENMIAK
HHCCCEEECHHHCCCCEEEEECCCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]