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The map label for this gene is cca [H]

Identifier: 154246501

GI number: 154246501

Start: 2853742

End: 2854653

Strand: Direct

Name: cca [H]

Synonym: Xaut_2560

Alternate gene names: 154246501

Gene position: 2853742-2854653 (Clockwise)

Preceding gene: 154246500

Following gene: 154246502

Centisome position: 53.75

GC content: 70.72

Gene sequence:

>912_bases
ATGGGCTTGCCATCGCGCCGGGGGTGGTGGTCCTATTCCCGGATGGACGAGACGCCCCCCTTCCGCATCGAGGACATGAT
CGAGACCCATCCCGGTCTCGCGCTGCTGCGCCTCGCCGCCCTTATCGCCGAGAGCAGCCCGAAGGACGCGCGCGCTGACG
AGGACACCCTCGACCTCATGTTCGAGCAGATGGAGGTGGGCGCGCTCGACGCTCTCGACCCCGTCCGCATGTGGGCGGAG
CTGGAGCGCGGGCTGATGGCCCCGGCCCCCGCCGCCATGGTGAAGGCCCTGCGTGAATGCGGGGTGCTGGAGATCGTGCT
GCCGGAGGTGGCCTCCCTGTTCGGCGTGCCGCACATCACCGATACGGCGGACGAGATCGACCTCGGCGTGCTGATCCTCA
ATTCCCTCACCGAGGCGGCCCGGCGCAACGCGCCGCTGGAGGTGCGGTTCGCGCTTCTGGTGATGCATGCGGGCAAGTCG
GATTCCCCGCGCGAGCACCTGCCGGTGCATTACCGCCACATGGAGCGCGGCCTGCCCCGCATCCACGCCATCTGCGACCG
CTTCGCCCTGCCGGACGTGTGCCGGGACCTCGCTTTGCTGGCGCTTCAGGAGTGCGAGCGGGTAGGGCGCGTGTCGCCCA
TCCGCGCCGGCCCGGTGGCGGCCATGCTGGAGCGCACCGGCGCCTTCCGCGATCCGGACCTGTTCGCCAAGCTGTTGCTG
GTCTCCACCTGCGACTACTTCGGCTACGGCGACAAGGAAGGCGACACCCACCCCAAGGTCGACCTCCTGCTCGCCGCCCG
CACCGCCTGCGCCGCGGTGGACGGCGAGGGGCTTGAGGGCGACGAGTTGGCCACCGCGCGGGCGCAGGCGATTGCGCTGG
CGTTCCGGTCGGAGCGGTGGGCGGAGGCTTGA

Upstream 100 bases:

>100_bases
CCTCGCCGACAGTCCCATGGGCGGGCTGCGGGCCACTGTGCGCATCCCGGTGTGAGGCCGGACCGGCCTCTATCGCTTAA
GTAATAGGCATGCCTTATTC

Downstream 100 bases:

>100_bases
GGGGATGCCTTGCCGGATGCGCGACGTTTCTTACATGGTAAGGTAAGAAATCCGGAGGCAGCCATGCGCATCACATCCAA
AGGGCAGGTGACCATTCCCG

Product: tRNA nucleotidyltransferase/poly(A) polymerase family protein

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase [H]

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MGLPSRRGWWSYSRMDETPPFRIEDMIETHPGLALLRLAALIAESSPKDARADEDTLDLMFEQMEVGALDALDPVRMWAE
LERGLMAPAPAAMVKALRECGVLEIVLPEVASLFGVPHITDTADEIDLGVLILNSLTEAARRNAPLEVRFALLVMHAGKS
DSPREHLPVHYRHMERGLPRIHAICDRFALPDVCRDLALLALQECERVGRVSPIRAGPVAAMLERTGAFRDPDLFAKLLL
VSTCDYFGYGDKEGDTHPKVDLLLAARTACAAVDGEGLEGDELATARAQAIALAFRSERWAEA

Sequences:

>Translated_303_residues
MGLPSRRGWWSYSRMDETPPFRIEDMIETHPGLALLRLAALIAESSPKDARADEDTLDLMFEQMEVGALDALDPVRMWAE
LERGLMAPAPAAMVKALRECGVLEIVLPEVASLFGVPHITDTADEIDLGVLILNSLTEAARRNAPLEVRFALLVMHAGKS
DSPREHLPVHYRHMERGLPRIHAICDRFALPDVCRDLALLALQECERVGRVSPIRAGPVAAMLERTGAFRDPDLFAKLLL
VSTCDYFGYGDKEGDTHPKVDLLLAARTACAAVDGEGLEGDELATARAQAIALAFRSERWAEA
>Mature_302_residues
GLPSRRGWWSYSRMDETPPFRIEDMIETHPGLALLRLAALIAESSPKDARADEDTLDLMFEQMEVGALDALDPVRMWAEL
ERGLMAPAPAAMVKALRECGVLEIVLPEVASLFGVPHITDTADEIDLGVLILNSLTEAARRNAPLEVRFALLVMHAGKSD
SPREHLPVHYRHMERGLPRIHAICDRFALPDVCRDLALLALQECERVGRVSPIRAGPVAAMLERTGAFRDPDLFAKLLLV
STCDYFGYGDKEGDTHPKVDLLLAARTACAAVDGEGLEGDELATARAQAIALAFRSERWAEA

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789436, Length=276, Percent_Identity=30.4347826086957, Blast_Score=119, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 33314; Mature: 33183

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLPSRRGWWSYSRMDETPPFRIEDMIETHPGLALLRLAALIAESSPKDARADEDTLDLM
CCCCCCCCCCCHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHH
FEQMEVGALDALDPVRMWAELERGLMAPAPAAMVKALRECGVLEIVLPEVASLFGVPHIT
HHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCC
DTADEIDLGVLILNSLTEAARRNAPLEVRFALLVMHAGKSDSPREHLPVHYRHMERGLPR
CCHHHHHHHHHHHHHHHHHHHCCCCHHEEHHHHHEECCCCCCCHHHCCHHHHHHHCCCHH
IHAICDRFALPDVCRDLALLALQECERVGRVSPIRAGPVAAMLERTGAFRDPDLFAKLLL
HHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHH
VSTCDYFGYGDKEGDTHPKVDLLLAARTACAAVDGEGLEGDELATARAQAIALAFRSERW
HHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
AEA
CCC
>Mature Secondary Structure 
GLPSRRGWWSYSRMDETPPFRIEDMIETHPGLALLRLAALIAESSPKDARADEDTLDLM
CCCCCCCCCCHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHH
FEQMEVGALDALDPVRMWAELERGLMAPAPAAMVKALRECGVLEIVLPEVASLFGVPHIT
HHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCC
DTADEIDLGVLILNSLTEAARRNAPLEVRFALLVMHAGKSDSPREHLPVHYRHMERGLPR
CCHHHHHHHHHHHHHHHHHHHCCCCHHEEHHHHHEECCCCCCCHHHCCHHHHHHHCCCHH
IHAICDRFALPDVCRDLALLALQECERVGRVSPIRAGPVAAMLERTGAFRDPDLFAKLLL
HHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHH
VSTCDYFGYGDKEGDTHPKVDLLLAARTACAAVDGEGLEGDELATARAQAIALAFRSERW
HHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
AEA
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA