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The map label for this gene is rpoE [H]

Identifier: 154245964

GI number: 154245964

Start: 2279217

End: 2279762

Strand: Reverse

Name: rpoE [H]

Synonym: Xaut_2021

Alternate gene names: 154245964

Gene position: 2279762-2279217 (Counterclockwise)

Preceding gene: 154245965

Following gene: 154245961

Centisome position: 42.94

GC content: 69.05

Gene sequence:

>546_bases
ATGGATGCCGCCGCCCTGCGCACGCAGGTGCTCGAATTCCTCCCGGCGCTGCGCGCCTTCGCGCGGTCGCTGACACGCAA
CCGCACGGAAGCCGACGACCTGGTGCAGGAAACCCTGCTCAAGGCCTTGTCCAACATGGACAAGTTCGATCCGGGCACGA
ACCTGCGCGCATGGCTCTTCACGATCCTGCGCAACACCTATTACACCGAGATCCGCAAGCGCCGCCGCGAGAACGACGGC
ATGAGCGCCCTGGCCCAGCAGGACACCAACATCGGTCCGAGCCAGGAATGGTCGGCGACCCTGTCCTCCCTCAAGGAGGC
CCTGGCCCGGCTGCCCGACGACCAGCGCGAGGCGCTGGTGCTGGTGGGCGCGGCGGGCCTGTCCTATGAGGAAGCGGCGG
AGGTGTGCGGCTGCGCGCTGGGCACCATCAAGAGCCGGGTGAACCGCGCCCGCGCCAAGCTGCTGGTGCTCATGGGCGCC
GAGAACACCGCCGACGTGGTGGAAGGTGACGCGGTCGCCTTGTCGGCCGCCCGCAGCGTCGGCTGA

Upstream 100 bases:

>100_bases
ACACCCTGCGTTGCCCCACCGTTTCGCCCGAAAGATAGGTCACCGAATGGACTTGCTCGCGGATCGCGAAGGGGATCTCC
CGGAAAGGGGTCGTCGAGAC

Downstream 100 bases:

>100_bases
TCGCGCTCCATCGCCGCCCGGGACGCTGACTTCGCTCAGTCGCCGCGCGGGCTTGGCGCTCCCCTCCTTCTCAGGCTCTG
ACCGCCGTCACGGCCGGGCT

Product: ECF subfamily RNA polymerase sigma-24 factor

Products: NA

Alternate protein names: Sigma-24 [H]

Number of amino acids: Translated: 181; Mature: 181

Protein sequence:

>181_residues
MDAAALRTQVLEFLPALRAFARSLTRNRTEADDLVQETLLKALSNMDKFDPGTNLRAWLFTILRNTYYTEIRKRRRENDG
MSALAQQDTNIGPSQEWSATLSSLKEALARLPDDQREALVLVGAAGLSYEEAAEVCGCALGTIKSRVNRARAKLLVLMGA
ENTADVVEGDAVALSAARSVG

Sequences:

>Translated_181_residues
MDAAALRTQVLEFLPALRAFARSLTRNRTEADDLVQETLLKALSNMDKFDPGTNLRAWLFTILRNTYYTEIRKRRRENDG
MSALAQQDTNIGPSQEWSATLSSLKEALARLPDDQREALVLVGAAGLSYEEAAEVCGCALGTIKSRVNRARAKLLVLMGA
ENTADVVEGDAVALSAARSVG
>Mature_181_residues
MDAAALRTQVLEFLPALRAFARSLTRNRTEADDLVQETLLKALSNMDKFDPGTNLRAWLFTILRNTYYTEIRKRRRENDG
MSALAQQDTNIGPSQEWSATLSSLKEALARLPDDQREALVLVGAAGLSYEEAAEVCGCALGTIKSRVNRARAKLLVLMGA
ENTADVVEGDAVALSAARSVG

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in heat shock and oxidative stress response; it is believed to control protein processing

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=131, Percent_Identity=33.587786259542, Blast_Score=60, Evalue=8e-11,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014293
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 19743; Mature: 19743

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS01063 SIGMA70_ECF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAAALRTQVLEFLPALRAFARSLTRNRTEADDLVQETLLKALSNMDKFDPGTNLRAWLF
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
TILRNTYYTEIRKRRRENDGMSALAQQDTNIGPSQEWSATLSSLKEALARLPDDQREALV
HHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHEEE
LVGAAGLSYEEAAEVCGCALGTIKSRVNRARAKLLVLMGAENTADVVEGDAVALSAARSV
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHCCHHHHHHHHHHC
G
C
>Mature Secondary Structure
MDAAALRTQVLEFLPALRAFARSLTRNRTEADDLVQETLLKALSNMDKFDPGTNLRAWLF
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
TILRNTYYTEIRKRRRENDGMSALAQQDTNIGPSQEWSATLSSLKEALARLPDDQREALV
HHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHEEE
LVGAAGLSYEEAAEVCGCALGTIKSRVNRARAKLLVLMGAENTADVVEGDAVALSAARSV
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHCCHHHHHHHHHHC
G
C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]