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The map label for this gene is pheS [H]

Identifier: 154245875

GI number: 154245875

Start: 2186648

End: 2187769

Strand: Reverse

Name: pheS [H]

Synonym: Xaut_1931

Alternate gene names: 154245875

Gene position: 2187769-2186648 (Counterclockwise)

Preceding gene: 154245876

Following gene: 154245874

Centisome position: 41.21

GC content: 66.76

Gene sequence:

>1122_bases
ATGAGCATCGACATGTCCGACATCGACGTGACCGACCAGGGCGCCATCACGGACCTGGAGCAAGACATCGCGAGCGCCAT
CCTGGGCGCCGAGGATGAAGCCGGCCTCGAAGAGGTGCGCATCGCCGCCCTCGGCAAGAAGGGCTCGGTCTCGGAGTTGC
TCAAGTCGCTGGGCACCATGAGCCCGGACCAGCGCAAGGTCATGGGCCCGGCCATCAACGGGCTGCGCGACCGGGTGACC
GGCCTCATCACCCATCGCCGCTCGGCGCTGAAGACCAAGGCCCTGGAAGCCCGCCTCGCCACCGAAAAGGTGGATGTGAC
CCTGCCGGTCCGCGAGGCCGCCGCCGAGACCGGCCGCGTCCATCCCATCGCCCAGGTGATGGAGGAGCTGACCGCCATCT
TCGCCGACATGGGCTTCTCGGTGGCGGAAGGCCCGGACATCGAGGACGACTTCCACAATTTCACCGCGTTGAACTTCCCC
CCCGGCCATCCGGCGCGGGAGATGCACGACACCTTCTTCCTGCCCCCCGGCGCCGACGGGCAGCGCAAGGTGCTGCGCAC
CCACACCTCTCCGGTGCAGGTGCGCACCATGCAGACCAAGACGCCGCCCATCCGCGTCATCTGCCCCGGTCGCACCTATC
GCTGCGACAGTGACCAGACCCACACCCCCATGTTCCACCAGGTGGAGGGGCTGGTGATCGACAAGGGCGCGCACCTCGGC
CACCTGAAGTGGATCCTGGAAGAATTCTGCAAGTCTTTCTTCGAGGTGGAGGGGGTGAAGATGCGCTTCCGCCCGTCCTT
CTTCCCCTTCACCGAGCCATCCATGGAAGTGGACATCCAGTGCGACCGCTCCCGCCCCGGCGAGATCCGCTTCGGCGAGG
GCTCGGACTGGCTGGAGATCCTCGGCTGCGGCATGGTGCACCCCAACGTGCTACGCAATTGCGGGCTCGACCCGGACGAA
TACCAGGGCTTTGCCTGGGGCATGGGCATCGACCGCATCGCCATGCTGAAATACGGCATGAGCGACCTGCGCGCCTTCTT
CGAGGCCGACGTGCGCTGGCTCTCCCACTACGGCTTCCGCCCGCTGGATTTCCCGACCCTCGCCGGCGGCCTGAGCGCGT
AA

Upstream 100 bases:

>100_bases
CATCCGTCCGGGGCTTTTGCGTTTGGGCGGCCCCCGTCTATAAGCGGCGCAACCGTCCAGGACCCGCGCCCGCCCGACCC
CGCGGGTGTTCCAGAACTTC

Downstream 100 bases:

>100_bases
GGAGCAGGACCCATGAAATTCACCTTCTCCTGGCTCCAGGACCACCTTGAGCCCACCGCCTCCTTCGATGCCATCGTCGA
GCGCCTGTCCCTCATCGGCC

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS [H]

Number of amino acids: Translated: 373; Mature: 372

Protein sequence:

>373_residues
MSIDMSDIDVTDQGAITDLEQDIASAILGAEDEAGLEEVRIAALGKKGSVSELLKSLGTMSPDQRKVMGPAINGLRDRVT
GLITHRRSALKTKALEARLATEKVDVTLPVREAAAETGRVHPIAQVMEELTAIFADMGFSVAEGPDIEDDFHNFTALNFP
PGHPAREMHDTFFLPPGADGQRKVLRTHTSPVQVRTMQTKTPPIRVICPGRTYRCDSDQTHTPMFHQVEGLVIDKGAHLG
HLKWILEEFCKSFFEVEGVKMRFRPSFFPFTEPSMEVDIQCDRSRPGEIRFGEGSDWLEILGCGMVHPNVLRNCGLDPDE
YQGFAWGMGIDRIAMLKYGMSDLRAFFEADVRWLSHYGFRPLDFPTLAGGLSA

Sequences:

>Translated_373_residues
MSIDMSDIDVTDQGAITDLEQDIASAILGAEDEAGLEEVRIAALGKKGSVSELLKSLGTMSPDQRKVMGPAINGLRDRVT
GLITHRRSALKTKALEARLATEKVDVTLPVREAAAETGRVHPIAQVMEELTAIFADMGFSVAEGPDIEDDFHNFTALNFP
PGHPAREMHDTFFLPPGADGQRKVLRTHTSPVQVRTMQTKTPPIRVICPGRTYRCDSDQTHTPMFHQVEGLVIDKGAHLG
HLKWILEEFCKSFFEVEGVKMRFRPSFFPFTEPSMEVDIQCDRSRPGEIRFGEGSDWLEILGCGMVHPNVLRNCGLDPDE
YQGFAWGMGIDRIAMLKYGMSDLRAFFEADVRWLSHYGFRPLDFPTLAGGLSA
>Mature_372_residues
SIDMSDIDVTDQGAITDLEQDIASAILGAEDEAGLEEVRIAALGKKGSVSELLKSLGTMSPDQRKVMGPAINGLRDRVTG
LITHRRSALKTKALEARLATEKVDVTLPVREAAAETGRVHPIAQVMEELTAIFADMGFSVAEGPDIEDDFHNFTALNFPP
GHPAREMHDTFFLPPGADGQRKVLRTHTSPVQVRTMQTKTPPIRVICPGRTYRCDSDQTHTPMFHQVEGLVIDKGAHLGH
LKWILEEFCKSFFEVEGVKMRFRPSFFPFTEPSMEVDIQCDRSRPGEIRFGEGSDWLEILGCGMVHPNVLRNCGLDPDEY
QGFAWGMGIDRIAMLKYGMSDLRAFFEADVRWLSHYGFRPLDFPTLAGGLSA

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758340, Length=273, Percent_Identity=32.6007326007326, Blast_Score=137, Evalue=2e-32,
Organism=Escherichia coli, GI1788007, Length=342, Percent_Identity=47.953216374269, Blast_Score=345, Evalue=2e-96,
Organism=Caenorhabditis elegans, GI32563657, Length=277, Percent_Identity=32.129963898917, Blast_Score=136, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17508957, Length=277, Percent_Identity=32.129963898917, Blast_Score=136, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI32566635, Length=263, Percent_Identity=30.0380228136882, Blast_Score=105, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6321087, Length=273, Percent_Identity=32.967032967033, Blast_Score=134, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6325304, Length=100, Percent_Identity=34, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI18858079, Length=269, Percent_Identity=31.5985130111524, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI17137424, Length=258, Percent_Identity=25.5813953488372, Blast_Score=83, Evalue=3e-16,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978 [H]

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 41391; Mature: 41260

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIDMSDIDVTDQGAITDLEQDIASAILGAEDEAGLEEVRIAALGKKGSVSELLKSLGTM
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCHHHHHHHHCCC
SPDQRKVMGPAINGLRDRVTGLITHRRSALKTKALEARLATEKVDVTLPVREAAAETGRV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECHHHHHHHCCCC
HPIAQVMEELTAIFADMGFSVAEGPDIEDDFHNFTALNFPPGHPAREMHDTFFLPPGADG
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHCCCEEECCCCCCCHHHHCCEEECCCCCCC
QRKVLRTHTSPVQVRTMQTKTPPIRVICPGRTYRCDSDQTHTPMFHQVEGLVIDKGAHLG
CHHHHHHCCCCEEEEEEECCCCCEEEEECCCCEECCCCCCCCCHHHHHCCEEEECCCCHH
HLKWILEEFCKSFFEVEGVKMRFRPSFFPFTEPSMEVDIQCDRSRPGEIRFGEGSDWLEI
HHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEEEEECCCCCCCEEECCCCCHHHH
LGCGMVHPNVLRNCGLDPDEYQGFAWGMGIDRIAMLKYGMSDLRAFFEADVRWLSHYGFR
HHCCCCCHHHHHHCCCCHHHCCCEEECCCHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCC
PLDFPTLAGGLSA
CCCCHHHCCCCCC
>Mature Secondary Structure 
SIDMSDIDVTDQGAITDLEQDIASAILGAEDEAGLEEVRIAALGKKGSVSELLKSLGTM
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCHHHHHHHHCCC
SPDQRKVMGPAINGLRDRVTGLITHRRSALKTKALEARLATEKVDVTLPVREAAAETGRV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECHHHHHHHCCCC
HPIAQVMEELTAIFADMGFSVAEGPDIEDDFHNFTALNFPPGHPAREMHDTFFLPPGADG
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHCCCEEECCCCCCCHHHHCCEEECCCCCCC
QRKVLRTHTSPVQVRTMQTKTPPIRVICPGRTYRCDSDQTHTPMFHQVEGLVIDKGAHLG
CHHHHHHCCCCEEEEEEECCCCCEEEEECCCCEECCCCCCCCCHHHHHCCEEEECCCCHH
HLKWILEEFCKSFFEVEGVKMRFRPSFFPFTEPSMEVDIQCDRSRPGEIRFGEGSDWLEI
HHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEEEEECCCCCCCEEECCCCCHHHH
LGCGMVHPNVLRNCGLDPDEYQGFAWGMGIDRIAMLKYGMSDLRAFFEADVRWLSHYGFR
HHCCCCCHHHHHHCCCCHHHCCCEEECCCHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCC
PLDFPTLAGGLSA
CCCCHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]